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Citations for Promega :
351 - 400 of 4716 citations for PCR Genotyping kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2021Quote: ... 10 μl 5x PCR buffer (Gotaq flexi, Promega), 8 μl MgCl ...
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bioRxiv - Cell Biology 2021Quote: ... a PCR was done using Taq polymerase (Promega) and visualize on agarose gel to assess xbp1 ...
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bioRxiv - Neuroscience 2022Quote: ... PCR was performed using Go Green Taq (Promega) according to the vendor protocol ...
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bioRxiv - Neuroscience 2022Quote: ... PCR products were ligated to pGEM-T (Promega) or pBluescript SKII (− ...
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Construction of a constitutively active type III secretion system for heterologous protein secretionbioRxiv - Synthetic Biology 2022Quote: ... Colony PCR was performed using GoTaq polymerase (Promega). The ΔhilE hilD::mCherry was constructed by incorporating hilD::mCherry construct into the ΔhilE strain.
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bioRxiv - Biophysics 2022Quote: ... the two purified flanking PCR fragments (Promega, USA) were assembled with BamHI and HindIII-digested PK18 (Gmr ...
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bioRxiv - Microbiology 2022Quote: ... Diagnostic PCR was performed using GoTaq polymerase (Promega) or OneTaq DNA polymerase.
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bioRxiv - Cancer Biology 2022Quote: ... PCR was performed in GoTaq Master Mix (Promega) according to standard procedures ...
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bioRxiv - Cell Biology 2020Quote: ... PCR was performed with GoTaq DNA polymerase (Promega); primer sequences are shown in supplemental Table S1 ...
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bioRxiv - Molecular Biology 2020Quote: ... we ligated PCR amplicons to pGEM-T (Promega) vector system and confirmed that all picked clones encompassed TATA box deletion and guide6 reconstruction using Sanger sequencing.
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bioRxiv - Zoology 2020Quote: ... 10 μl 5x PCR buffer (Gotaq flexi, Promega), 8 ul MgCl ...
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bioRxiv - Zoology 2020Quote: ... 10 μL 5× PCR buffer (Gotaq flexi, Promega), 8 μL 25mM MgCl ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR products were cloned into pGEM-T (Promega) and sequenced ...
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bioRxiv - Microbiology 2020Quote: ... PCR was performed with GoTaq Master Mix (Promega) with 1ul cDNA template at 1:20 dilution ...
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bioRxiv - Bioengineering 2021Quote: ... and cleaned again using PCR cleanup (Promega, A6754). Plasmid DNA inputs were used in a set of concentrations ranging from 0.5 nM to 10 nM ...
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bioRxiv - Molecular Biology 2022Quote: ... with mutagenesis performed by PCR using PfU (Promega). All mutagenesis was confirmed by Sanger Sequencing (Source Bioscience).
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bioRxiv - Synthetic Biology 2022Quote: ... and 300 μM of PCR Nucleotide mix (Promega). The liposome suspensions were incubated at 30 °C in a thermocycler for the indicated time periods.
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bioRxiv - Plant Biology 2022Quote: ... PCR was performed using GoTaq DNA Polymerase (Promega) with a melting temperature of 55 °C for SpCas9 ...
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bioRxiv - Neuroscience 2022Quote: ... PCR was conducted using GoTaq DNA polymerase (Promega). Primer sequences were as follows ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR fragments were subcloned into pGEM-Teasy (Promega) and then moved as HindIII/NotI fragments into pCB4699 ...
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bioRxiv - Physiology 2022Quote: ... qRT-PCR was performed using SYBR Green (Promega). The primers used were (5’-3’ ...
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bioRxiv - Molecular Biology 2022Quote: ... PCRs were performed using GoTag DNA polymerase (Promega) and primers annealing either of the flanking exons to look at the pattern of splicing or exon-junction (EJ ...
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bioRxiv - Zoology 2022Quote: ... 10 μl 5x PCR buffer (Gotaq flexi, Promega), 8 μl MgCl ...
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bioRxiv - Zoology 2023Quote: ... 10 μl 5x PCR buffer (Gotaq flexi, Promega), 8 μl MgCl ...
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bioRxiv - Microbiology 2023Quote: ... Diagnostic PCR was performed using GoTaq polymerase (Promega).
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bioRxiv - Physiology 2023Quote: ... PCR amplification used GoTaq Hot Start Polymerase (Promega). PCR conditions were 95°C for 3 min ...
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bioRxiv - Zoology 2024Quote: ... 10 μl 5x PCR buffer (Gotaq flexi, Promega), 8 μl MgCl ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR fragments were cloned in pGEM-T (Promega), and the inserts were sequenced by Sanger sequencing (University of California Berkeley DNA Sequencing Facility) ...
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bioRxiv - Neuroscience 2024Quote: ... GoTaq quantitative PCR (qPCR) Master Mix (Promega; A6002) was used to conduct the qPCR ...
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bioRxiv - Microbiology 2024Quote: PCR was performed using GoTaq Master Mix (Promega) using 5 ng of DNA extracted from RS collected after growth on MHA containing 0 or 32 μg/mL cefiderocol ...
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bioRxiv - Genomics 2024Quote: ... PCR products were cloned into pCI-neo (Promega) downstream of the Luciferase2 gene under the control of a CMV promoter ...
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bioRxiv - Developmental Biology 2024Quote: ... and also with PCR Master Mix (M7502, Promega), Taq polymerase (EP0402 ...
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bioRxiv - Developmental Biology 2021Quote: ... The PCR reaction mixture was prepared for a 10 μL reaction volume using a GoTaq® DNA Polymerase PCR mix (Promega): 2 μL 5X Green GoTaq® Reaction Buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... The PCR reaction mixture was prepared for a 10 μL reaction volume using a GoTaq® DNA Polymerase PCR mix (Promega): 2 μL 5X Green GoTaq® Reaction Buffer ...
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bioRxiv - Microbiology 2020Quote: ... Duplicate PCR products from the samples were pooled and purified with the Wizard® Gel and PCR Clean-Up System (Promega), ligated and subsequently cloned into E ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR products were directly sequenced using PCR primers after purification with Gel Clean-up System (Promega Corporation, Madison, WI, USA). All sequencing reactions were done on ABI 3700 DNA automated sequencer with the BigDye chemistry (Applied Biosystems ...
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bioRxiv - Cell Biology 2021Quote: ... Real time quantitative PCR was performed in a CFX 96 BioRad CFX 96 Real-Time PCR detection instrument with GoTaq qRT-PCR master mix (Promega, A6002). The sequence of the primers used in this study are ...
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bioRxiv - Microbiology 2021Quote: ... The resulting PCR amplicon from the second-round PCR was gel-purified and ligated to the pGEMT- Easy vector (Promega, USA), as per the manufacturer-recommended protocol (Promega ...
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bioRxiv - Cell Biology 2019Quote: ... were generated using PCR amplification of two DNA fragments and subsequent assembly PCR (using Pfu DNA polymerase from Promega, Mannheim, Germany) with the artificially inserted exon 9 sequence as region for annealing ...
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bioRxiv - Microbiology 2023Quote: ... Each PCR reaction with a total volume of 25 μL contained 12.5 μL of PCR master mix (Promega; Madison, WI, USA), 8.0 μL of nuclease free water ...
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Phosphoproteomics of cellular mechanosensing reveals NFATC4 as a regulator of myofibroblast activitybioRxiv - Systems Biology 2023Quote: ... Quantitative PCR (qPCR) was then performed on LightCycler 480 Real-Time PCR System using the GoTaq qPCR Master Mix (Promega, #A6001), and the primers pairs are listed in Table S7 ...
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bioRxiv - Plant Biology 2023Quote: Stem-loop PCR and 5’-RLM-RACE PCR amplification products were purified using The Wizard® SV Gel and PCR Clean-Up System (Promega). Cloning of the different PCR products was performed as described in the pGEM®-T Vector system according to the instructoŕs instructions (Promega) ...
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bioRxiv - Microbiology 2023Quote: ... The three PCR fragments were ligated by PCR using primers P1/P3 and cloned into pGEM®-T easy vector (Promega). Plasmid was then digested with NotI ...
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bioRxiv - Biochemistry 2023Quote: ... The linearized pMRNAXP vector and the PCR fragments of DNA encoding spike were cleaned up by Wizard® SV Gel and PCR Clean-Up System (Promega). The PCR fragments of spike protein DNA were cloned into linearized pMRNAXP vector using In-Fusion® HD Cloning Kit (Clontech® Laboratories ...
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bioRxiv - Molecular Biology 2024Quote: ... and gene expression was determined by quantitative PCR (QuantStudio 6 Pro Real-Time PCR System) with GoTag qPCR System (Promega, A6002). Actb and Rplp0 were used as housekeeping genes ...
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bioRxiv - Cancer Biology 2020Quote: ... RT-PCRs were conducted in two-step reactions by using two-step RT-PCR: M-MLV reverse transcriptase in M-MLV RT-PCR system (Promega, Madison, WI) and Platinum Taq polymerase (GeneMark ...
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bioRxiv - Genetics 2019Quote: The target genomic region was amplified using PCR and purified using Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI). The DNA sequence of the PCR fragment was determined using cycle sequencing with BigDye v3.1 reagents (Applied Biosystems ...
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bioRxiv - Evolutionary Biology 2023Quote: ... one microliter volumes of Reverse Transcription reactions were used as template for amplification of internal cDNA fragments using in 20 μl volume PCR reactions (35 rounds of amplification using Promega 2X PCR Master Mix (Promega, Madison, WI). Gene-specific primers and annealing temperatures are listed in Table 1 ...
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bioRxiv - Plant Biology 2023Quote: ... The 16S rDNA PCR-amplified fragments were purified by Wizard® SV Gel and PCR Clean-Up System (Promega, New Delhi, India) and digested by HaeIII (SRL ...
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bioRxiv - Cell Biology 2020Quote: ... mCherry and CLIP were PCR amplified from pFN21A (Promega), pSNAPf (NEB) ...