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Citations for Promega :
351 - 400 of 1011 citations for Neuronal acetylcholine receptor subunit alpha 5 NACHRA5 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 5 µg ml-1 Sequencing Grade Modified Trypsin (Promega). Peptides were eluted with 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... at 5 µM in Tracer Dilution Buffer (Promega N291B) was added to all wells ...
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bioRxiv - Cell Biology 2023Quote: ... 5% glycerol) with protease inhibitor mixture without EDTA (Promega), followed by the addition of 0.3% CHAPS ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 10 µL coelenterazine h (Promega, 5 µM final concentration) was added ...
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bioRxiv - Genetics 2024Quote: ... 5 µL of GoTaq G2 Green Master Mix (Promega), and 200 µM of primers ...
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bioRxiv - Neuroscience 2024Quote: ... 5 µl of 5X Green GoTaq Buffer (Promega, M791A), 0.2 µl of GoTaq DNA polymerase (Promega ...
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bioRxiv - Cancer Biology 2021Quote: ... lentiviral packaging vectors into human HEK-293T cells via calciumphosphate transfection (Promega; Madison, WI, USA), according to manufacturer’s directions ...
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bioRxiv - Cancer Biology 2021Quote: The 3’UTR of human PAQR3 was cloned into pmirGLO vector (Promega, Madison, WI, USA), and then validated by sequencing ...
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bioRxiv - Molecular Biology 2021Quote: ... Human SMARCB1 was translated in vitro using TNT Quick Coupled Transcription/Translation System (L1170, Promega). 1 µg of pcDNA3.1-FLAG-SMARCB1 was incubated at 30°C for 90 minutes with 20 µM methionine and TNT T7 Quick Master Mix ...
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bioRxiv - Cancer Biology 2020Quote: ... Human cell lines were DNA fingerprinted for provenance using the Power-Plex 1.2 kit (Promega) and confirmed to be the same as the DNA fingerprint library maintained by ATCC ...
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bioRxiv - Bioengineering 2021Quote: The open reading frame for human SOX17 was PCR amplified using GoTaq Master Mix (Promega) from the PB-TRE3G-SOX17 plasmid (Table S5) ...
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bioRxiv - Molecular Biology 2020Quote: ... 2.5ng ml-1 recombinant human fibroblast growth factor (rhFGF) (Promega, Cat#G5071, Madison, WI, USA), and 1% chicken embryonic extract (Seralab ...
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bioRxiv - Genetics 2022Quote: ... human POPDC1 and POPDC2 cDNA sequences were cloned into the pFC14K or pFC32K plasmids (Promega), which contain C-terminus sequences for HaloTag and NanoLuc tags ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 μL of GoTaq® Probe qPCR Master Mix (Promega) and 0.5 μL of the appropriate TaqMan Assay (20X ...
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bioRxiv - Molecular Biology 2021Quote: ... and 5 μl of RNasin Plus RNase Inhibitor (Promega, N2611) and incubated for 2 hours at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... Trypsin (Promega; 5 ng/mL in 20 mM ammonium bicarbonate) was added to the gel pieces and proteolysis was allowed to proceed overnight at 37 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... RNase inhibitor at 5% of the reaction volume (RNasin, Promega), and T4 DNA ligase (4,000 U per 50 µL reaction ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 5 U/μl GoTaq Flexi DNA Polymerase (Promega, WI, USA), and 1 μl of DNA template ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and 5 μL 2x buffer for T4 DNA Ligase (Promega) were mixed and incubated at 55°C for 10 min ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.05 μl GoTaq DNA Polymerase (concentration 5 u/μl) (Promega), 2 μl 5X colorless GoTaq reaction buffer (containing 7.5 mM MgCl2 ...
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bioRxiv - Microbiology 2020Quote: ... 20 μl of 5 ng/μl sequencing grade trypsin (Promega), 50mM AHC ...
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bioRxiv - Biochemistry 2020Quote: ... 5 mM MgCl2 with 0.3 µg/ul Acetylated BSA (Promega). All RNAs used were labelled with 5’ FAM (IDT ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 5 μL/well of CellTiter-Glo 2.0 reagent (Promega G9241) was added to the wells of the 384-well plate and allowed to equilibrate for 10 minutes before luminescence intensity was recorded on a Tecan SPARK plate reader (luminescence module ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM DTT and 5 μg/μl of trypsin (Promega)) for 3 min at 30°C ...
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bioRxiv - Genetics 2022Quote: ... 5-mins after the addition of furimazine NanoLuc substrate (Promega). A range of expression ratios of the NanoLuc and HaloTag containing constructs within the cells was achieved by varying the proportion of each plasmid during transfection ...
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bioRxiv - Plant Biology 2022Quote: ... and 5 μL of RNasin Plus RNase Inhibitor (Promega, N2611) and incubated for 2 h at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... The Nano-Glo® 5× reagent (furimazine substrate + buffer; Promega) was prepared and added to each sample ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 μL 5 ng/μL sequencing grade trypsin (Promega; V5111) prepared in 50 mM ABC supplemented with 1 mM CaCl2 were added to the gel pieces and incubated 30 min at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... A solution containing 5 μg of sequencing grade trypsin (Promega) in 100 mM triethylammonium bicarbonate (TEAB ...
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bioRxiv - Cell Biology 2023Quote: ... a mix was prepared consisting of 5 % PEG-8000 (Promega), 100 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Microbiology 2023Quote: ... 5 μL GoTaq Colorless Master Mix (Promega Corporation, Madison, Wisconsin) including 200 μM of each dNTP and 1 unit Taq DNA polymerase ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were then treated with 5 units of DNase (Promega) and incubated for an additional 30 min at 37°C before being loaded on a 15% urea-polyacrylamide gel ...
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bioRxiv - Cell Biology 2024Quote: ... 5 μl of M-MLV Reverse Transcriptase Buffer (Promega #M531A), 10 mM NTPs mixture and 200 U of MMLV (Promega #M170A ...
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bioRxiv - Biochemistry 2024Quote: ... 5 µl/well NanoBRET™ Nano-Glo® Substrate (Promega) and Extracellular NanoLuc® Inhibitor (Promega ...
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bioRxiv - Plant Biology 2023Quote: ... 10 µL of 5× Green GoTaq® Reaction Buffer (Promega), 1 µL of dNTP Mix (10 mM each of dTTP ...
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bioRxiv - Plant Biology 2023Quote: ... 10 µL of 5× Colorless GoTaq® Reaction Buffer (Promega), 1 µL of dNTP Mix (10 mM each of dTTP ...
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bioRxiv - Molecular Biology 2020Quote: Human Genomic DNA was extracted from the blood using the Wizard Genomic DNA Purification kit (Promega) as per the instructions.
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bioRxiv - Immunology 2021Quote: ... 25 μl of 7.5×104 Jurkat effector cells expressing either human FcγRIIIa or murine FcγRIV (Promega) resuspended in assay buffer were added to each well ...
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bioRxiv - Molecular Biology 2021Quote: Full-length human RNU6 sequence (110 nucleotides) was amplified by PCR and cloned into psiCHECK2 (Promega), downstream of Renilla luciferase ...
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bioRxiv - Neuroscience 2021Quote: Genomic DNA (gDNA) was extracted from human frontal cortex using Wizard Genomic DNA Purification Kit (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Standards for quantification were created by serial dilution of pre-quantified BSC human male DNA (Promega). Amplification reactions were performed in duplicate using 96 well-plates using a CFX96 Touch Real-time PCR Detection System (Bio-Rad).
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bioRxiv - Cancer Biology 2020Quote: The region of interest (750-850 bp) was PCR amplified from pooled male human DNA (Promega) and cloned into a STARRseq luciferase validation vector_ORI_empty plasmid (Addgene plasmid #99298 ...
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bioRxiv - Molecular Biology 2020Quote: ... WT or mutant promoter of human Rpl28 gene was cloned into pGL-3-basic (Promega, E1751). The number is relative to transcriptional start site ...
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bioRxiv - Bioengineering 2020Quote: ... and detected with anti-rabbit or anti-human HRP-conjugated secondary antibodies (Promega W4011 and W4031). Blots were developed using ECL (ThermoScientific 32106 ...
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bioRxiv - Immunology 2023Quote: ... 100 μl of 5,000-fold diluted Peroxidase AffiniPure goat anti-human IgG (H+L) antibody (Promega) was added into each well and incubated for 1 h at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Human and murine NLRC5 and CXCL10 promoters were cloned into pGL3 basic luciferase reporter vector (Promega). All mutated plasmids were constructed from wild type plasmids by Fast Mutagenesis System (TransGen Biotech) ...
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bioRxiv - Cell Biology 2023Quote: ... 10mM Ultrapure ATP and 0.7 μg of recombinant full-length human CDC7/DBF4 kinase (Promega, V5088). Reactions were incubated at 37 °C for 120 min and then terminated by freezing on dry ice before mass spectrometry.
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bioRxiv - Microbiology 2021Quote: ... and the near full-length 16S rRNA gene was amplified using primers 8F (5’-AGAGTTTGATCCTGGCTCAG-3’) and 1492R (5’-TACGGTTACCTTGTTACGA-3’) with the GoTaq® Hot Start Colorless Master Mix (Promega Corp., Madison, WI, USA). PCR was performed using Eppendorf Vapo Protect Mastercycler Pro S 6325 (Hamburg ...
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bioRxiv - Genomics 2021Quote: ... 1 μL DNA and 5 μL of Master Mix 2x (Promega) (0.3 units of Taq Polymerase ...
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bioRxiv - Neuroscience 2019Quote: ... and 5 μL of CellTiter-Fluor Cell Viability Assay reagent (Promega) was added to each well ...