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Citations for Promega :
351 - 400 of 1343 citations for Dnase Free and Rnase Free Distilled Water since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Genomic DNA was eliminated by incubating 2 μg of RNA with 2 U of RQ1 RNase-free DNase I (Promega) for 30 min at 30°C ...
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bioRxiv - Molecular Biology 2021Quote: Total RNAs from 15 wild type and 15 mutant zebrafish embryos were extracted using TRI reagent (MRC) and treated with RQ1-RNase free DNase I (Promega) and Proteinase K (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 µg of this RNA was treated for 10 minutes at 65 °C with a unit of RQ1 RNAse-free DNase (Promega) in a total reaction volume of 10 µl ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 ug (for cellular RNA) and 20 ng (for EV RNA) was treated with 1 U of DNAse I RNAse-free (#EN0521 PROMEGA) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... using the TRIzol method (as described by the manufacturer) An aliquot of total RNA was treated with RQ1 RNase-free DNAse (Promega), to avoid genomic contamination and 1μl of total RNA was quantified by spectrophotometer using a Nanodrop 1000 (Thermo Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... 1 μg of total RNA was incubated with RNase-free DNase I (1 unit/μl) following the protocol provided by the manufacturer (Promega), followed by reverse-transcription into first-strand cDNA using ImProm II reverse transcriptase (Promega) ...
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bioRxiv - Molecular Biology 2023Quote: ... Trace amounts of contaminating genomic DNA were removed by treating all RNA samples with RNAse-free DNase I prior to use for cDNA synthesis according to the manufacturer’s instructions (Promega, WI).
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bioRxiv - Plant Biology 2024Quote: ... 5.5 μg of the same total RNA used for the RNA-seq libraries were first treated with RQ1 RNase-free DNase (Promega) to confirm (via negative controls ...
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bioRxiv - Plant Biology 2024Quote: ... First-strand cDNA was synthesized from half a volume of total RNA (1 μg) treated for 30 min at 37°C with RQ1 RNase-free DNase (Promega) using a SuperScript III First-Strand Synthesis System (Invitrogen) ...
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bioRxiv - Pathology 2021Quote: ... The cDNA was diluted in nuclease-free water (Promega, USA) and used for real-time PCR with either SARS-CoV-2 or GAPDH specific primers ...
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bioRxiv - Pathology 2022Quote: ... The cDNA was diluted in nuclease-free water (Promega, USA) and used for real-time PCR with either SARS CoV-2 or 18S rRNA specific primers ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 uL of nuclease free water or RNasin (Promega, N2611). qRT-PCR reactions were set up in 10 μL volume containing 4 μL cDNA ...
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bioRxiv - Microbiology 2024Quote: ... A non-template control consisting of nuclease free-water (Promega Corporation ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was diluted 1:1 with nuclease-free water (Promega). Quantitative real-time PCR reactions were carried out using the SYBR Green PCR master mix and ROX Passive Reference Dye (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... 22 μl of nuclease-free water) (#20034197, Illumina; #G9441, Promega) for incubattion at 37°C with shaking at 300 rpm for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... 3 µl of nuclease free water (Promega, Cat No. P119E), and 5 µl of cDNA using the following program ...
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Synchronous 3D patterning of diverse CNS progenitors generates motor neurons of broad axial identitybioRxiv - Neuroscience 2024Quote: ... Double elution with 20- 30µl of Nuclease-free Water (Promega) was performed ...
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bioRxiv - Developmental Biology 2020Quote: ... Total RNA was eluted with 25 μl of RNase-free water and was quantified using the Quantifluor RNA System (Promega, #E3310).
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bioRxiv - Neuroscience 2024Quote: ... Isolated RNA was treated with RQ1 RNA-free DNAse (Promega) to ensure removal of contaminating genomic DNA prior to amplification ...
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bioRxiv - Genomics 2021Quote: ... Total RNA was extracted from cultures in mid-log phase using a standard hot phenol method and treated with RNase-free DNase RQ1 (Promega, M6101) to remove any DNA contamination ...
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bioRxiv - Microbiology 2024Quote: ... Contaminating DNA in the samples were removed through incubation at 37°C for 2 h using RNase-free DNase I (Promega, USA). All RNAs in the samples were converted into cDNA using a cDNA EcoDry Premix (Clontech ...
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bioRxiv - Microbiology 2023Quote: ... 1% RNAse-free BSA (Gemini) and 1:400 RNasin Plus (Promega) before storage at -80°C ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was diluted in DNase-free water and used for qRT-PCR with Go Taq® qPCR Master Mix reagent (PROMEGA) and analyzed in a Step-OnePlus real-time PCR system (Applied Biosystems) ...
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bioRxiv - Physiology 2020Quote: ... and eluted into 20 μl of nuclease- free water (P1195; Promega). All libraries were prepared from 100 to 200 pg of cDNA using the Nextera XT Kit (Illumina ...
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bioRxiv - Cancer Biology 2021Quote: ... 19.5µl of nuclease-free water) (FC-121-1030, Illumina; G9441, Promega). Transposition reactions were incubated at 37°C for 30 minutes in an Eppendorf ThermoMixer with agitation at 1000 RPM ...
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bioRxiv - Cell Biology 2024Quote: ... 22 μl of nuclease-free water) (Illumina, #20034197 and Promega, #G9441) at 37°C for 30 minutes with 300 rpm shaking ...
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bioRxiv - Genetics 2021Quote: ... and 50 million nuclei per liver were incubated in a volume of 950 ul with 32 U of RQ1 RNase-free DNase I (Promega, cat. # M6101) for 2 min at 37°C ...
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bioRxiv - Genomics 2023Quote: ... traces of genomic DNA were removed by treating 600 ng of total RNA with 1 µl RQ1 RNase-Free DNase (Promega, cat# M6101) in 9 µl reaction additionally containing RQ1 DNase buffer and 1 µl of murine RNase inhibitor at 37°C for 45 minutes ...
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bioRxiv - Microbiology 2024Quote: ... The RNA samples were treated with DNAse (RQ1 Rnasa-Free Dnase, Promega, Madison, WS, USA) and retrotranscribed with FireScript KIT Reverse Transcriptase (Solis BioDyne ...
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bioRxiv - Microbiology 2020Quote: ... All RNA samples were treated with RQ1 RNase-free DNaseI (Promega, USA), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... residual DNA was removed by RNase-free RQ1 DNaseI (Promega, Madison, WI) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Residual DNA was removed by RNase-free RQ1 DNaseI (Promega, Madison, WI) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and the RNA was precipitated with an equivalent volume of isopropanol and incubated at 55°C for 10 minutes before being resuspended in 50 μL of RNase-free water (Promega, Madison, WI, USA). We determined the concentrations of RNA using a Smart-Spec plus spectrophotometer (Bio-Rad) ...
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bioRxiv - Plant Biology 2024Quote: ... DNA digestion (RQ1 RNAse-free DNAse) and reverse transcription (GoScript™ Reverse Transcription) were performed as described by the manufacturer (Promega, Madison, USA). qPCR was performed (qPCR Master Mix plus CXR ...
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bioRxiv - Molecular Biology 2021Quote: ... Nuclease-free water was used for non-template control (Promega, Madison, WI). The reaction was performed in a Rotor-Gene 600 thermocycler (Corbett Research ...
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bioRxiv - Microbiology 2022Quote: ... vacuum-dried and dissolved in nuclease-free water (Promega, Madison, WI, USA). DNA solutions were stored at –20°C until use.
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bioRxiv - Microbiology 2022Quote: ... Two μg RNA was subsequently treated with 2U RNA-free DNAse (Promega) following the manufacturers’ instructions ...
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bioRxiv - Plant Biology 2024Quote: ... DNA-free total RNA was prepared by incubation with RQ1 DNase (Promega) and RNAsin for 15 min ...
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bioRxiv - Microbiology 2020Quote: ... and RNA was resuspended in 50 μl of nuclease free water (Promega, P119A). Illumina 150 bp PE libraries were prepared using the TruSeq Stranded Total RNA-seq Kit in combination with the Illumina Ribozero Yeast Kit ...
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bioRxiv - Cancer Biology 2024Quote: ... The RNA was dissolved in 20-40 µl nuclease-free water (Promega, P119E), quantified using NanoDrop® Spectrophotometer ND-1000 (Isogen Life Science ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were washed twice (3000 x g for 5 min at 4°C) and resuspended in RNAse-free PBS with 1% BSA and 0.5 U/ul RNase inhibitor (Cat. N2615, Promega). Cells were then used for the 10x Genomics Chromium Single Cell System (10x Genomics ...
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bioRxiv - Neuroscience 2021Quote: ... cells were washed twice (3000 x g for 5 min at 4°C) and resuspended in RNAse-free PBS with 1% BSA and 0.5 U/ul RNase inhibitor (Cat. N2615, Promega). Cells were then used for the 10x Genomics Chromium Single Cell System (10x Genomics ...
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bioRxiv - Molecular Biology 2021Quote: ... all RNA samples were treated with DNaseI enzyme (RQ1 RNase-free DNaseI, Promega, USA) for 60 min at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... 30 µL of nuclease-free water with RNAsin (100 U/mL) (Promega, Madison, WI) was added and incubated at 95°C for 1 min ...
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bioRxiv - Microbiology 2020Quote: ... and 7.4 µL nuclease-free water according to the GoTaq qPCR Master Mix (Promega) protocol ...
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bioRxiv - Genomics 2021Quote: ... 930 µL of nuclease-free water and supplemented with 0.2 U/ul RNaseIN Plus (Promega) and 0.1U/ul Superasin (ThermoFisher Scientific) ...
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bioRxiv - Plant Biology 2020Quote: ... Pooled RNA samples derived from each conditions were subjected to DNaseI digest (RNase-free DNaseI, Promega) in the presence of RNase inhibitor (RNasin Plus ...
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bioRxiv - Cancer Biology 2024Quote: ... distilled water) containing 0.1% NP40 and 0.1% Tween-20 and 0.01% Digitonin (Promega, G9441) and incubated for 3 min to allow cells permeabilization ...
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bioRxiv - Biochemistry 2022Quote: ... eluted in 12 µl of nuclease free water and quantify using the dsDNA QuantiFluor Dye System (Promega, #E2670).
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bioRxiv - Cancer Biology 2022Quote: ... 20,000 to 50,000 cells were lysed in the transposase reaction mix (12.5 μl 2xTD buffer, 2 μl TDE1 [Illumina], 10.25 μl nuclease-free water, and 0.25 μl 1% digitonin [Promega]) for 30 min at 37 °C ...