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Citations for Promega :
351 - 400 of 4458 citations for Cow Protein L Isoaspartate PCMT1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... the protein suspension was digested with 4 μg trypsin (Promega) in 40 μL NH4HCO3 (50 mM ...
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bioRxiv - Neuroscience 2023Quote: ... the proteins were digested by trypsin (Promega, Madison, WI, USA), and peptides were extracted from the gel with an acetonitrile/formic acid solution ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were enzymatically digested with 0.5 ug Trypsin Gold (Promega) for 16 h ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were digested overnight with sequencing-grade modified trypsin (Promega). Then ...
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bioRxiv - Bioengineering 2023Quote: ... several concentrations of purified recombinant HaloTag protein standard (Promega, G4491) were labeled with ligand and run in duplicate to generate a calibration curve ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested overnight at 37°C with trypsin (Promega) and Lys-C (Wako,125-05061 ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were digested with 6 ng/µL trypsin (Promega, UK) overnight at 37 °C ...
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bioRxiv - Genetics 2023Quote: ... proteins in the plugs were digested overnight with trypsin (Promega) at 37°C in a 25-mM NH4HCO3 buffer (0.2 µg trypsin in 20 µL) ...
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bioRxiv - Plant Biology 2023Quote: ... alkylation (55 mM CAA) and protein digestion (trypsin-gold, Promega) the resulting peptides were purified using self-packed StageTips (C18 material ...
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bioRxiv - Immunology 2023Quote: ... Proteins were cleaved overnight with 1 µg of trypsin (Promega) at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were then incubated with Lys-C/trypsin mixture (Promega) (200 ng for pull-down samples and 1 ug for total lysate samples ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were digested in 50 mM TEAB containing trypsin (Promega) at a 1:20 enzyme-to-protein ratio overnight at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were digested with 1µg trypsin (Trypsin Gold, Promega Corporation) overnight at 37°C in a shaker at 1250 rpm ...
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bioRxiv - Cell Biology 2024Quote: ... the protein suspension was digested with 2 µg trypsin (Promega) overnight at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: Kit (Promega). For transposition with an additional plasmid pCas12k ...
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bioRxiv - Molecular Biology 2022Quote: ... The urea concentration was then diluted with 17 μl of 50 mM HEPES pH 8.5 and after adding 1 μl of 0.2 mg/ml trypsin (Promega) in 0.2 mM HCl the digestion continued overnight at 37°C with interval mixing at 2000 RPM for 30 s every 15 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed with PBST and then anti-mouse IgG (H&L) alkaline phosphatase-conjugated secondary antibody (Promega) was incubated with the membrane at a 1:5,000 dilution for 1 h at RT ...
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bioRxiv - Neuroscience 2020Quote: 80,000 HEK293T cells were plated in 500μl HEK Media and 24 hrs later 500ng total plasmid DNA was transfected with 1.5μl FuGene HD (Promega) as follows ...
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bioRxiv - Biophysics 2022Quote: ... The poly-L-lysine coated side of the cover slip was exposed to 5 mg/ml streptavidin (Promega) in this space for 15 min ...
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bioRxiv - Microbiology 2022Quote: ... mutations were generated in a fragment of the L ORF subcloned into a pGEM T easy vector (Promega). The mutated ORF fragment was sequenced and then substituted in the L ORF of pTM1 ...
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bioRxiv - Cancer Biology 2022Quote: ... 20,000 to 50,000 cells were lysed in the transposase reaction mix (12.5 μl 2xTD buffer, 2 μl TDE1 [Illumina], 10.25 μl nuclease-free water, and 0.25 μl 1% digitonin [Promega]) for 30 min at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... The samples were diluted with 17 µL of 50 mM HEPES (pH 8.5) and digested with 1 µL of 0.2 mg/mL trypsin (Promega) in 0.2 mM HCl at 37°C with interval mixing at 2000 RPM for 30 sec every 15 min.
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bioRxiv - Molecular Biology 2019Quote: ... for 30 minutes at room temperature and then digested by LysC (Lysyl endopeptidase, #125-05061, Wako, ratio LysC/protein 1:100) and trypsin (Trypsin Gold, V5280, Promega, ratio trypsin/protein 1:20) overnight ...
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bioRxiv - Biochemistry 2019Quote: ... 70 000-100 000 cells (in 200-450 μl DMEM) were seeded into each well containing a transfection cocktail of 0.4 μl of Fugene 6 (E2693, Promega), with 20 μl Opti-MEM (11058-021 ...
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bioRxiv - Cell Biology 2019Quote: ... were transfected with 1 µg of DNA and 4 µL FuGENE 6 in 100 µL Opti-MEM media for 24-36 hours according to the manufacturer’s instructions (Promega). Cells were fixed with 4% (v/v ...
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bioRxiv - Plant Biology 2020Quote: ... 10 mg/L BSA and 1 mM PMSF) and assayed using the Dual-Luciferase® Reporter Assay System (Promega). Experiments were normalized to negative control set at 1.
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bioRxiv - Pathology 2021Quote: ... and 20 µg/mL L-012) and recorded by a Promega GloMax Navigator microplate luminometer (Promega Biotech, Beijing, China). GraphPad Prism software was used to analyze data.
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bioRxiv - Molecular Biology 2022Quote: ... and purified on a 10g/L agarose gel using the Wizard SV Gel and PCR Clean-Up system (Promega). All mixtures were made in 1x NEBuffer 3.1 on ice with final concentrations of 80nM Cas9 ...
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bioRxiv - Plant Biology 2022Quote: ... Luciferase activity of cell extracts was measured with Sirius L Tube Luminometer (Berthold) and the luciferase assay system (Promega).
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bioRxiv - Genomics 2021Quote: The supernatant was magnetically removed and the beads were resuspended in 20 µl of L3 DNA linker ligation mixture (8 µl water, 5 µl 4X ligation buffer, 1 µl RNA ligase [New England Biolabs], 0.5 µl RNasin [N2615, Promega GmbH] ...
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bioRxiv - Cell Biology 2021Quote: ... the supernatant was removed and the nuclei pellet was resuspended into 500 µl nuclei storage buffer (NSB: 1x PBS; Invitrogen, 0.5% RNase free BSA;Serva, 1:200 RNaseIN plus inhibitor; Promega, 1x EDTA-free protease inhibitor ...
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bioRxiv - Cell Biology 2022Quote: ... were seeded onto 6-well plates coated with 0.1 mg ml−1 poly-L-lysine and transfected using FuGENE HD transfection reagent (Promega) with a mixture of third-generation lentiviral backbone vector pJHUMCS that encodes BDNF-pHluorin ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then diluted with 120 μL of PBS before 1.2 μL of ProteaseMax surfactant (0.1 mg/mL in 100 mM ammonium bicarbonate, Promega, V2071) and sequencing grade trypsin (10 μL ...
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bioRxiv - Neuroscience 2023Quote: ... Then the beads were incubated with 80 μL of 2 M urea in 50 mM Tris-HCL containing 1 mM DTT and 0.4 μg trypsin (Promega) at 25 °C for 1 hour while shaking at 1,000 rpm ...
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bioRxiv - Microbiology 2023Quote: ... 20 μL of MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) inner salt (Promega) was added to each well and the plate was further incubated for 1 h at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins in the excised band were digested with Trypsin (Promega, V5280). The resulting peptides were analyzed online by nano-flow HPLC-nanoelectrospray ionization using a Qexactive HFX mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: TNT® SP6 High-Yield Wheat Germ Protein Expression System (Promega) was used to perform in vitro translation assay ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were digested with 10ng/ul of sequencing grade trypsin (Promega) overnight at 37C ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were then digested using modified trypsin (Promega, P/N V5113) at a 1:20 (w/w ...
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bioRxiv - Immunology 2019Quote: ... Proteins were digested with sequencing grade trypsin (1:100; Promega, USA) overnight at 37°C and peptides concentration was checked by spectrophotometry (Nanodrop 2000 ...
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bioRxiv - Microbiology 2021Quote: ... Crosslinked proteins were digested by the addition of 1μg trypsin (Promega) and overnight incubation at 30°C ...
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bioRxiv - Neuroscience 2020Quote: ... The protein digestion was performed by adding 0.2 µg LysC (Promega) in 50 µL of 100 mM ABC for 3h at 1200 rpm at 37°C followed by overnight trypsin digestion using 0.2 µg of trypsin (Promega ...
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bioRxiv - Neuroscience 2020Quote: ... Fusion proteins were expressed by TnT® Quick Coupled system (Promega). 15 μL of HA tagged protein was then incubated with candidate GST-coated beads at 4°C for 2 hours ...
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bioRxiv - Cell Biology 2021Quote: ... Protein digestion was carried out with sequencing grade modified Trypsin (Promega) at 1/50 protease/protein (wt/wt ...
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bioRxiv - Biochemistry 2021Quote: ... the proteins were digested by addition of sequencing-grade trypsin (Promega) at an enzyme-to-substrate ratio of 1:25 ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were digested with 500 ng trypsin (Promega, Madison, USA) overnight at 37°C on a shaking incubator ...
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bioRxiv - Plant Biology 2021Quote: ... Expressed proteins were directly captured using Magne Halo Tag Beads (Promega). The protein-bound beads were incubated with adaptor-ligated gDNA fragments ...
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bioRxiv - Cell Biology 2021Quote: ... The proteins were on-bead digested with 1 µg trypsin (Promega) for 4 hours at 37 °C under agitation ...
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bioRxiv - Cell Biology 2021Quote: ... Then the protein was digested with sequencing grade modified trypsin (Promega) (1 ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were then digested with 12.5 ng/uL modified trypsin (Promega) in 50 μL 100 mM ammonium bicarbonate ...