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Citations for Promega :
3901 - 3950 of 4574 citations for 6 CHLORO 2 METHYLIMIDAZO 1 2 A PYRIDINE 3 CARBOXYLIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... Samples were diluted 1:4 with 50 mM Tris/HCl (pH 8.0) and sequencing grade modified trypsin (Promega) was added in an enzyme-to-substrate ratio of 1:50 ...
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bioRxiv - Cancer Biology 2023Quote: ... Luciferase gene expression in E0771 and RM-1 cells was validated using a luciferase activity assay kit (Promega). Luciferase-labeled E0771 and RM-1 cells were maintained in RPMI 1640 media supplemented with 10% FBS ...
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bioRxiv - Plant Biology 2023Quote: The substrate of the nano-luciferase diluted at 1:100 (v/v) (furimazine, Kit from Promega (ref 1110)) was spread on seedling that grew on Agar plates ...
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bioRxiv - Physiology 2023Quote: ... GLP-1 was detected by indirect sandwich amide chemiluminescence ELISA using a luminescence plate reader (GloMax Promega, USA). This total GLP-1 assay detects GLP-1 (7-36 ...
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bioRxiv - Systems Biology 2024Quote: ... RNA was refolded by adding 88.8 μL 300 mM sodium cacodylate and 1 μL RNasin Plus (Promega N2611) and 10 min incubation at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: 20×106 cells were collected in cold PBS by scraping and lysed in RIP buffer (251mM Tris-HCl pH 7.4, 1501mM KCl, 5mM EDTA, 0.5% Nonidet P-40, 201U1ml−1 RNAsin (Promega), 0,51mM dithiothreitol (DTT ...
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bioRxiv - Microbiology 2023Quote: ... and then incubated with anti-rabbit horseradish peroxidase (HRP) conjugate antibodies (1:20000 dilution) (Promega, Madison, WI, USA) for two hours at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... AePer50 reverse transcription (RT) and qPCR reactions were performed using GoTaq® 1-Step RT-qPCR System (Promega), with 10 µl of 2X qPCR Master Mix ...
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bioRxiv - Cancer Biology 2023Quote: ... The secondary antibodies used in this study were 1:2,500-diluted HRP-conjugated anti-mouse IgG (Promega, #W4021) and anti-rabbit IgG (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... A 90 μl volume of each culture and 10 μl of the furimazine (1 in 200 dilution; Promega) were incubated in a Cellstar non-transparent white 96-well microplate for one h and the bioluminescence intensity was monitored with a Tecan Infinite 200 PRO plate reader every 30 s ...
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bioRxiv - Systems Biology 2023Quote: ... and digested according to the manafacturer’s protocol for 1 hour at 50 °C by Trypsin Platinum (Promega, WV), digested material was dried in speedvac (Eppendorf ...
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bioRxiv - Biochemistry 2023Quote: ... Tryptic digestion was performed by incubating the beads with 1 µg MS-grade TPCK trypsin (Promega, Madison, WI) dissolved in 200 µL of 50 mM ammbic (pH 8.3 ...
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bioRxiv - Microbiology 2023Quote: ... Standard curves were generated using purified genomic stocks (HSV-1 bacterial artificial chromosome and human genome Promega #G1471). Absolute copy number of genomic stocks was determined using droplet digital PCR (Biorad QX600) ...
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bioRxiv - Biochemistry 2024Quote: ... were digested overnight at 37 °C using trypsin with a 1:20 trypsin to sample ratio (Promega, #V5117). The next day ...
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bioRxiv - Microbiology 2023Quote: Real-time PCR was carried out using the Promega GoTaq® Probe 1-Step RT-qPCR System (Promega) with Sarbecovirus-specific envelope gene primers from bat RNA samples as published by (Corman ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 μg of total RNA was used for first strand cDNA synthesis with M-MLV reverse transcriptase (Promega) and oligo (dT ...
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bioRxiv - Developmental Biology 2024Quote: ... From there on all buffers were supplemented with 1:100 RNasin Plus RNase inhibitor (Promega, Cat. No. N2615).
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bioRxiv - Cancer Biology 2024Quote: ... G17-1 cell proliferation was measured by MTS assay (Cell Titer 96 Aqueous One, Promega, Madison, WI, USA).
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bioRxiv - Bioengineering 2024Quote: The primary antibodies used in this study were the following: rb-α-Nanoluc (non-commercial, Promega, 1:1000); rb-α-CD63 (ab68418 ...
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bioRxiv - Molecular Biology 2024Quote: ... samples were incubated with 80 μL of 2 M urea in 50 mM Tris-HCl (pH 7.5) containing 1 mM DTT and 0.5 μg trypsin (Promega) at 25°C with shaking for pre-digestion ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was generated from 1 μg total RNA per sample using the M-MLV Reverse Transcriptase kit (Promega). To detect RNA transcript of targeted genes ...
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bioRxiv - Synthetic Biology 2024Quote: ... pre-ran reaction solutions were incubated with 1 µL RNase A (4 mg/mL RNAse A solution, Promega), and 1 µL RNase One (10 units/µL RNase ONE Ribonuclease solution ...
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bioRxiv - Plant Biology 2020Quote: ... First-strand cDNA was synthesized from 1 μg total RNA using the M-MLV Reverse Transcriptase (Promega, Cat. #M1705) (primers are listed in Supplementary Table 8) ...
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bioRxiv - Cancer Biology 2021Quote: ... The digested vector (200 ng) was ligated with 1 µL of the oligonucleotides (0.45nmol/μl) using T4 DNA ligase (Promega) for 3 hrs ...
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bioRxiv - Microbiology 2020Quote: ... ratio 50:1 (protein:rLysC) for 3h/37°C and then digested with Sequencing Grade Modified Trypsin (#V5111, Promega, France) ratio 50:1 (protein:trypsin ...
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bioRxiv - Microbiology 2020Quote: ... Cytosine tails (C-tails) were added to 1 µg sheared DNA using terminal deoxynucleotidyl transferase (TdT, Promega, Madison, WI). Transposon containing fragments were amplified in a PCR containing DNA from the TdT reaction as template and primers specific to the ColE1 site on the 5’ end of the transposon ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were washed twice (3000 x g for 5 min at 4°C) and resuspended in RNAse-free PBS with 1% BSA and 0.5 U/ul RNase inhibitor (Cat. N2615, Promega). Cells were then used for the 10x Genomics Chromium Single Cell System (10x Genomics ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.05% (v/v) NP-40 and 0.5% (v/v) Triton X-100) containing 20 U ml−1 of RNasin (Promega), 1 mM DTT ...
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bioRxiv - Neuroscience 2021Quote: ... cells were washed twice (3000 x g for 5 min at 4°C) and resuspended in RNAse-free PBS with 1% BSA and 0.5 U/ul RNase inhibitor (Cat. N2615, Promega). Cells were then used for the 10x Genomics Chromium Single Cell System (10x Genomics ...
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bioRxiv - Molecular Biology 2021Quote: ... Fujifilm) and then incubated for 8 h at 37 °C with sequence-grade trypsin (1□g; Promega, Madison, Wisconsin). Peptides were desalted on Ultra Micro Spin Columns C18 (The Nest Group Inc. ...
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bioRxiv - Bioengineering 2019Quote: ... Samples were then digested with mass-spectrometry grade trypsin (1:20, Trypsin:Substrate, Promega Gold Trypsin, San Luis Obispo, CA) overnight at 37°C [66] ...
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bioRxiv - Molecular Biology 2019Quote: ... Complementary DNA (cDNA) was synthesized from 1 μg of total RNA using random primers and Reverse Transcriptase System (Promega). The cDNA was used as a template for the real-time PCR amplification using PowerUp SYBR® Green Master Mix and a StepOne Plus system (Applied Biosystem ...
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bioRxiv - Plant Biology 2019Quote: ... Secondary goat anti-rabbit IgG conjugated with HRP was used at a dilution of 1:10,000 (Promega, Madison, WI).
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bioRxiv - Cancer Biology 2019Quote: ... The sample was then diluted 50% with PBS before sequencing grade trypsin (1 μg per sample, Promega Corp, V5111) was added for an overnight incubation at 37 °C ...
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bioRxiv - Synthetic Biology 2019Quote: ... The samples were diluted to 1% SDC using 100mM ammonium bicarbonate and mass spectrometry grade Trypsin (Promega, WI, USA) was added at a ratio of 1:50 (µg Trypsin:µg Protein ...
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bioRxiv - Microbiology 2021Quote: ... 1 μL of forward and reverse primer (5 μM, see Supplementary Table 1) and 5.5 μL of Nuclease-free Water (Promega). The PCR I was performed as follows ...
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bioRxiv - Immunology 2021Quote: Total RNA was extracted from sorted cells with RiboEx (GeneAll) and 0.2–1 μg was subjected to cDNA synthesis using the GoScript Reverse Transcription system (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The 1/10 of the sample was kept for injection and the 9/10 were digested with trypsin (Promega) (12.5 ng/µl ...
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bioRxiv - Plant Biology 2020Quote: ... 10 mg/L BSA and 1 mM PMSF) and assayed using the Dual-Luciferase® Reporter Assay System (Promega). Experiments were normalized to negative control set at 1.
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bioRxiv - Synthetic Biology 2021Quote: ... 1 µl of this crude lysate was used for PCR in a 10 µl GoTaq Green reaction (Promega M7123) with 0.25 µM of primers ...
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bioRxiv - Developmental Biology 2020Quote: ... Postnatal testes chemically synchronized for undifferentiated spermatogonia were homogenized at 2% w/v in polysome buffer (50 mM Tris, pH 7.4, 100 mM KCl, 12 mM MgCl2, 1% Nonidet P-40, 1mM DTT, 200 U/mL Promega RNasin ...
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bioRxiv - Biochemistry 2021Quote: ... The next day the sample was further diluted to 1 M urea and 750 ng Glu-C (Promega, V1651) were added and incubated for another 8 hours at 37°C.
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bioRxiv - Neuroscience 2021Quote: ... the supernatant was removed and the nuclei pellet was resuspended into 500ul nuclei storage buffer (NSB: 1x PBS; Invitrogen, 0.5% RNase free BSA;Serva, 1:200 RNaseIN plus inhibitor; Promega, 1x EDTA-free protease inhibitor ...
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bioRxiv - Cell Biology 2021Quote: ... membranes were subjected to three wash periods for 5 minutes in 1x TBS with 0.5% Tween and then probed with horseradish peroxidase–conjugated goat anti-mouse or goat anti-rabbit secondary antibodies at 1:2,500 (W4021, W4011, Promega) in 1x TBS with 5% nonfat dry milk and 0.5% Tween at room temperature for 1 hour ...
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ARL3 Mediates BBSome Ciliary Turnover by Promoting Its Outward Diffusion through the Transition ZonebioRxiv - Cell Biology 2021Quote: ... Five micrograms of RNA were reverse transcribed at 42 °C for 1 h using M-MLV Reverse Transcriptase (Promega) and oligo(T)18 primers (Takara) ...
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bioRxiv - Systems Biology 2021Quote: ... Then urea concentration was diluted to 1.5 M with HPLC water and 1:50 (w/w) trypsin (Promega GmbH) was added for 12 h at 37 °C with mixing at 700 rpm ...
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bioRxiv - Plant Biology 2020Quote: ... and incubated with an anti-rabbit immunoglobulin horseradish peroxidase-conjugated secondary antibody (1:4000 dilution, lot no. 187662; Promega) to detect SOG1-Myc ...
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m7G cap-eIF4E interaction stimulates polysome formation by enhancing first-round initiation kineticsbioRxiv - Biophysics 2021Quote: ... The translation reaction mixture for single-molecule detection was assembled according to the manufacturer’s instructions with the following modifications: 1) the RNase inhibitor RNaseIn (Promega) was used at a final concentration of 0.8 U/ul ...
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bioRxiv - Microbiology 2021Quote: ... Protein was reduced with 5 mM DTT and alkylated with 14 mM iodoacetamide followed by digestion with trypsin (1:100, w:w) (Promega) as described in [80] ...
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bioRxiv - Microbiology 2021Quote: ... 0.025% deoxycholate and 200 µg.mL−1 RNase A at 37°C for 20 min to lyse the cells and Protein Precipitation Solution (Promega) was added ...