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Citations for Promega :
3601 - 3650 of 4270 citations for 7 Bromo 1 2 3 4 tetrahydronaphthalen 1 ol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Luminescence was initiated by addition of a mixture of 2 μM coelenterazine-h (Promega) and 40 μM 3-isobutyl-1-methylxanthine (IBMX ...
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bioRxiv - Physiology 2021Quote: ... allowing measurement of 2-deoxyglucose-6-phosphate using the Glucose Uptake-Glo assay (Promega) and a FLUOstar Omega luminescence plate reader.
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bioRxiv - Microbiology 2020Quote: ... RNA (2 μg) was used for cDNA synthesis with M-MLV Reverse Transcriptase (Promega). Conventional PCR was used with the primer (Table 1 ...
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bioRxiv - Immunology 2021Quote: ... F(ab′)2 fragments were generated after cleavage of IgG with IdeS protease (Promega) and then purified using TALON metal affinity resin (Takara ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were transfected with 2 μg of the respective guideRNA plasmids using FuGene (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... with 2 uM Ponasterone A overnight and incubated with JF646 HaloTag ligand (Promega, GA1120) 15 minutes before imaging ...
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bioRxiv - Plant Biology 2023Quote: ... Two (2) μg of total RNA were used for cDNA synthesis with MMLV (Promega) and random hexamers (Invitrogen) ...
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bioRxiv - Plant Biology 2024Quote: ... 2 mg of the extracted RNA underwent treatment with RQ1 RNase-Free DNAse (Promega) before being used for cDNA synthesis with SuperScript IV Reverse Transcriptase (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... or a combination of 2 μg of trypsin and 0.2 μg of LysC (Promega). Samples were incubated at 37 °C overnight ...
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bioRxiv - Microbiology 2024Quote: ... for 2 h at 25 °C and digested with trypsin (Promega, 37 °C, overnight) in TEAB buffer (50 mM ...
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bioRxiv - Microbiology 2023Quote: ... and TCID50 equivalents determined using a SARS-CoV-2 E gene detection kit (Promega) based upon the Berlin primer/ probe set (70).
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bioRxiv - Molecular Biology 2023Quote: ... purified RNAs were additionally incubated with 2 units of RQ1-DNAse (Promega, cat# M6101) per 1 µg of RNA at 37°C for 30 min to eliminate plasmid DNA contamination ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA was generated from 2 µg isolated RNA using M-MLV reverse transcriptase (Promega) and diluted 1:15 for use in 12 µL qPCR reactions run on the StepOnePlus™ system (Applied Biosystem) ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were digested for 18 h at 37 °C with 2 μg trypsin (Promega). After digestion ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 µg total RNA served as a template using M-MLV Reverse Transcriptase (Promega) and random hexamer primers following manufactureŕs protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... including NCI were spiked with 2 ng of Luciferase Control RNA (LUC) (Promega, USA). These extracts were used for real-time quantitative PCR (RT-qPCR ...
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bioRxiv - Molecular Biology 2023Quote: ... and 24 hours later co-transfected with 500 ng psiCHECK™-2 Vector (Promega) containing the 3’UTR of human PLIN2 gene downstream to the Renilla luciferase gene ...
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bioRxiv - Cell Biology 2023Quote: ... and proteins were further digested by the addition of 2 μg trypsin (Promega #V5113) for 4 hours at room temperature on a shaker ...
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bioRxiv - Biochemistry 2023Quote: ... peptides were collected as described in ProteaseMax application note (Promega, TB373, Rev. 2/15), and purified using Pierce C18 Spin Tips (Thermo Fisher PI84850) ...
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bioRxiv - Cell Biology 2024Quote: ... for 20ms with 2 pulses or lipid-mediated delivery using FuGENE HD (Promega; TM328) according to the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2024Quote: ... HaloTagged AP-2 σ2 was visualized by adding Janelia Fluor 646-HaloTag ligand (Promega). Ligand (100 nM ...
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bioRxiv - Cancer Biology 2021Quote: MAGI2-AS3 or FOXN3 3’UTR containing miR-452-5p wild-type (WT) or mutant (MUT) binding sites were inserted into psiCHECK vector (Promega, USA). 293T cells were transfected with the constructed luciferase plasmid together with miR-NC or miR-452-5p mimics using Lipofectamine 2000 ...
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bioRxiv - Molecular Biology 2019Quote: ... the cells were transfected with 700 ng of plasmid construct (PIB/V5_Gr9) and 3 μl of FuGENE® HD transfection reagent (Promega, USA) in 100 μl of medium per well ...
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bioRxiv - Cell Biology 2020Quote: ... at 3500 cells/well and cell proliferation over 3-5 day periods was determined by measuring luminescence with CellTiter-Glo® assay kit (G7570, Promega), according to manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: A DNA fragment corresponding to partial f5 or f3a cDNAs was amplified from ovarian cDNAs by PCR using the gene-specific primers (see Supplemental Table 3 for detail) (28) and cloned into the pGEM-T Easy vector (Promega, USA). Correct sequence and orientations of inserts were validated by Sanger sequencing ...
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bioRxiv - Biochemistry 2022Quote: The crRNA (5’-AAUUUCUACUGUUGUAGAUGUGAUAAGUGGAAUGCCAUGUGGG-3’) was synthesized and purified using the T7 RiboMAX™ Express Large Scale RNA Production System (Promega). The following DNA templates were used for the in vitro transcription reaction ...
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bioRxiv - Microbiology 2021Quote: ... Remaining ATP was readout after 3 h incubation using Kinase-Glo® Luminescent Kinase kit following manufacturer’s instructions (Promega, WI, USA).
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bioRxiv - Cell Biology 2021Quote: ... of miR-148a putative binding sites reporter plasmids were constructed using the circMRPS35 and 3’-UTR of STX3 sequences in the psiCHECK2 vector (Promega, USA). For IRES activity analysis ...
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bioRxiv - Cell Biology 2020Quote: Transfections were carried out with 1 μg of total DNA (500 ng for each construct or with empty expression plasmid) and 3 μl of FuGENE ® HD Transfection Reagent (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... supernatant was harvested from Calu-3 cells and inactivated by addition of lysis buffer (Maxwell 16 viral total nucleic acid purification kit, Promega #AS1150) complemented with proteinase K ...
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bioRxiv - Microbiology 2021Quote: The cell viability of Vero E6 and Calu-3 cells was analyzed by quantification of ATP levels using the CellTiter-Glo™ Luminescent Cell Viability assay (Promega) according to the instructions of the manufacturer ...
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bioRxiv - Developmental Biology 2020Quote: ... with an added 5’-CACC-3’ at 5’-end and 5’-AAAC-3’ at 5’-end of the complementary strand were annealed in 10xT4 ligation buffer (Promega M1801) by continuous cooling from 95°C to 25°C ...
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bioRxiv - Plant Biology 2022Quote: ... the coding sequence of RE02 extended by BsaI cleavage sites (Supplementary Table 3) was cloned into the pGEM®-T vector (Promega). The cauliflower mosaic virus 35 promoter ...
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bioRxiv - Zoology 2024Quote: ... The amplicon target was amplified from WNV cDNA using the qPCR primers with the forward primer flanked by T7 sequence (5’-TAATACGACTCACTATAGGGATTCGGGAGGAGACGTGGTA-3’) and transcribed using T7 RiboMAX Express Large Scale RNA Production System kit (Promega, France). RNA was purified by ethanol precipitation ...
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bioRxiv - Neuroscience 2024Quote: The short TMEM106B 3’ UTR and the long TMEM106B 3’ UTR were amplified from human genomic DNA (H1) and then cloned into the pmirGLO Dual-Luciferase Vector (Promega, E1330) using Gibson assembly ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... drugs were added to plates in triplicate and incubated for 3 days before being developed by adding CellTiterGlo (Promega, Madison, WI) per manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: 10 μL in vitro nLuc mRNA translation reactions were performed in the dynamic linear range using 3 nM mRNA (31) in the Flexi Rabbit Reticulocyte Lysate (RRL) System (Promega # L4540) with final concentrations of reagents at 20% RRL ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were generated with pCS2-N-terminal 5×MYC vectors and pCS2-N-terminal 3×HA vectors described above using TnT® Coupled Reticulocyte Lysate System under the SP6 promoter (L4600, Promega) and by following manufacturer’s recommendations with few modifications ...
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bioRxiv - Molecular Biology 2023Quote: 10 μL in vitro translation reactions were performed in the linear range using 3 nM mRNA in the Flexi Rabbit Reticulocyte Lysate (RRL) System (Promega # L4540) with final concentrations of reagents at 30% RRL ...
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bioRxiv - Biophysics 2024Quote: ... labeling of TRPV1exCellHalo was completed in the imaging chamber by incubating cells with 3 μM Alexa660 Halo Ligand for 5 minutes (Promega, WI) in HBR followed by 3 minutes of continuous rinsing with HBR perfusion.
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... Infectivity was determined after 3 days by luminescence using a Promega GloMax plate reader following addition of BrightGlo reagent (Promega #E2620) to Calu-3 cells or FLuc Assay buffer (Nanolight #318 ...
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bioRxiv - Molecular Biology 2024Quote: ... and transfected with 1 μg of “driver” plasmid (pTMO2F3, p5UThORF2 or other “driver”) + 3 μg of “reporter” plasmid (pAluneoTet, or other “reporters”) using 12 μL FuGENE® 6 (Promega); the following day ...
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bioRxiv - Molecular Biology 2024Quote: ... approximately 1×106 cells were plated and transfected with 1 μg of “driver” plasmid (pTMO2F3, p5UThORF2 or other) + 3 μg of “reporter” plasmid (pJBM560) using 12 μL FuGENE® 6 (Promega). Transfection efficiency determination ...
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bioRxiv - Biochemistry 2024Quote: Each biological replicate was split into two 500 uL aliquots and incubated with either 3 µg of trypsin (Promega, sequencing grade) (“surface digest” ...
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bioRxiv - Cancer Biology 2020Quote: ... The mixture was added with 25 mM NH4HCO3 to dilute urea to 1 M followed by overnight digestion with sequencing-grade trypsin (Promega, Madison, WI, USA) at an enzyme/protein ratio of 1:40 (wt/wt ...
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bioRxiv - Immunology 2021Quote: ... the aqueous phase was added to 1 volume of 70% ethanol and loaded on ReliaPrep™ RNA Tissue Miniprep column (Promega, Cat #Z6111). Total RNA was isolated according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... and the first-strand cDNA was synthesized from 1 μg of total RNA with oligo dT or random primers using ImProm-II reverse transcriptase (Promega, Madison, WI, USA). The qPCR reactions were performed in a 20 μL reaction mixture using SYBR Green PCR Master Mix (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... were seeded into 24-well plate and cultured to 80% confluence when they were co-transfected with 4 μg of either TOP flash or FOP flash vector and 1 μg of pRL-TK (Renilla-TK-luciferase vector, Promega, Madison, WI, USA) using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 µl of the RT product (diluted 1:5) was used for semi-quantitative PCR or qPCR reactions with Promega PCR Mix (Promega, Madison, Wisconsin, USA) and SYBR Green PCR Mix (Applied Biosystems ...
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bioRxiv - Molecular Biology 2019Quote: The concentration and quality of proteins was assessed with a Bradford assay of the supernatant and confirmed with 12% SDS-PAGE.100 mM TEAB was used to dilute 100μg of protein solution with 8M urea that was subjected to digestion using Trypsin Gold (40:1: protein: enzyme, Promega, Madison, WI, USA) at 37°C overnight ...