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Citations for Promega :
3551 - 3600 of 6667 citations for Osteopontin Human OPN ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The luciferase activity of cell lysates was measured using a firefly luciferase assay kit (Promega, USA), according to manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... Plasmid and genomic DNA (gDNA miniprep kits were purchased from Promega. E. coli NEB 5α (NEB) was used as a host for plasmids ...
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bioRxiv - Plant Biology 2024Quote: ... the genomic DNA from Escherichia coli was purified following the Wizard Genomic DNA Purification Kit (Promega) protocol and served as a template for PCR amplification ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR products were purified using the Wizard SV Gel and PCR clean-up system kit (Promega) and used for RNA probe synthesis ...
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bioRxiv - Cell Biology 2024Quote: ... Cell viability was assessed using the CellTiter-Glo Luminescent Cell Viability Assay Kit (Promega, Cat. # G7570) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: Eluted viral DNA was quantified using Quantus Fluorometer with the QuantiFluor ds DNA system kit (Promega). Minimum concentration of DNA that was considered as sufficient was 1 ng/μl and samples with lower dsDNA concentration were excluded ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR products were digested with DpnI and further purified with a PCR clean-up kit (Promega). The R11 p2-p3 PCR product was then cloned into the pUC57 backbone with Gibson Assembly (50 ng pUC57 backbone and 50 ng p2-p3 insert) ...
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bioRxiv - Biochemistry 2021Quote: ... followed by a second dilution step to ~1 M urea with 50 mM NH4HCO3 and addition of trypsin (Promega, 1:50, 37 °C, overnight). After overnight incubation ...
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bioRxiv - Immunology 2022Quote: ... and digested over-night with Lys-C-Trypsin mix (1:100 enzyme to protein ratio) and trypsin (Promega; 1:50 enzyme to protein ratio). Following the digestion step ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit and mouse anti-βgal 1:500 (Cappel, Promega, Abcam), mouse anti acetylated tubulin 1:100 (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... and cleaned from excess DNA with DNAse 1 enzyme (Promega). RNA samples were reverse transcribed to cDNA and an anchor sequence at the variable part of the TCR was added using single strand ligation ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The reaction contained 1 unit of GoTaq DNA polymerase (Promega), 1X enzyme Flexi Buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... from Santa Cruz Biotechnology, mouse anti-β-Galactosidase (Z378A, 1:500) from Promega, mouse anti-MYC tag (#2276 ...
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bioRxiv - Microbiology 2020Quote: ... 1 μg total RNA and 0.5 μg random primers (Promega) were used with the GoScript™ Reverse transcriptase (Promega ...
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bioRxiv - Microbiology 2021Quote: ... 1 μg total RNA and 0.5 μg random primers (Promega) were used with the GoScript Reverse transcriptase (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... or goat anti-rabbit IgG at 1:3000 (Promega W4011). Washes were with high-salt TBST (50 mM Tris pH 7.5 400 mM NaCl 0.1% Tween-20).
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bioRxiv - Microbiology 2022Quote: ... 1 μg total RNA and 0.5 μg random primers (Promega) were used with the GoScript™ Reverse transcriptase (Promega ...
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bioRxiv - Neuroscience 2021Quote: ... 1 μL 3.5 mM Alexa Fluor 660 HaloTag ligand (Promega) was added to 200-300 μL cells and incubated at 30°C for 5 minutes ...
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bioRxiv - Microbiology 2019Quote: ... 3 μg of RNA was treated with DNase 1 (Promega) for 2 hours at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 μg of RNA was treated with DNase I (Promega) and reverse transcribed with Moloney murine leukemia virus reverse transcriptase RNase H(− ...
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bioRxiv - Genetics 2020Quote: ... and 1 ng/10ml rhFGF (Promega; Madison, WI; Cat# G5071). Cells were seeded at a density of 1.5×106 per 10 cm2 plate or 2-chamber slides (Corning Inc. ...
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bioRxiv - Microbiology 2021Quote: ... 1 μl RNasin Plus RNase inhibitor (40 U/μl, Promega) and 6.5 μl saturated trehalose] was added and incubated for 1 h ...
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bioRxiv - Systems Biology 2020Quote: ... followed by trypsin (Promega; enzyme-to-substrate ratio, 1:20)-mediated proteolysis using the same cycling scheme for 90 cycles ...
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bioRxiv - Biochemistry 2020Quote: ... and 1 μl GoScript™ reverse transcriptase (Promega cat. # A5003) was added ...
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bioRxiv - Biochemistry 2020Quote: ... T7 RNA polymerase and 1 μL RNasin RNase inhibitor (Promega) and incubated overnight at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... HRP-conjugated anti-mouse IgG (Promega, WI; 1:3000-5000), HRP-conjugated anti-rabbit IgG (Promega ...
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bioRxiv - Biochemistry 2020Quote: ... HRP-conjugated anti-rabbit IgG (Promega, WI; 1:3000-5000), HRP-conjugated anti-goat IgG (Santa Cruz ...
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bioRxiv - Cell Biology 2021Quote: ... and digested with 1 µg MS-grade trypsin (Gold, Promega) by overnight incubation at 37°C ...
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bioRxiv - Plant Biology 2021Quote: ... P40 pellets were treated with 1 μg/mL trypsin (Promega) in the presence or absence of 1% (v/v ...
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bioRxiv - Developmental Biology 2021Quote: ... and HRP-labelled secondary antibodies (RRID:AB_430833; RRID:AB_430834; Promega; 1:2,000) were used ...
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bioRxiv - Biochemistry 2021Quote: ... and 150 mM NaCl) containing protease inhibitor cocktail (1×) (Promega), followed by cell lysis by incubation on ice for 20 min ...
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bioRxiv - Cell Biology 2021Quote: ... HaloTag (1:1000 dilution in 0.5% milk, Promega, Cat#: G9211), GAPDH (1:100,000 dilution in 0.5% milk ...
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bioRxiv - Microbiology 2020Quote: ... 1 μl dNTP Mix (10 mM; Promega, Leiden, the Netherlands), 1 U of Phusion Green High-Fidelity DNA Polymerase (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... or anti-mouse IgG-HRP conjugate (1:10,000; Promega W402B) by incubating membranes for 2 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... 1 µg of pGL4.33[luc2P/SRE/Hygro] Vector DNA (Promega), and indicated amounts of human Scrib constructs (pCDNA3-eGFP-Scrib ...
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bioRxiv - Microbiology 2021Quote: ... in a 3:1 ratio of FuGENE®HD (Promega) transfection reagent to DNA ...
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bioRxiv - Neuroscience 2020Quote: ... β III tubulin (anti-mouse, dilution 1:20,000, Promega, Madison, WI), and NR2B (anti-rabbit ...
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bioRxiv - Cell Biology 2020Quote: ... and digested with 1 µg of sequencing-grade trypsin (Promega) for 2 h at 37°C followed by the addition of another 1 µg aliquot of the enzyme and incubation at 37°C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM EDTA) containing 10 µg trypsin (sequencing grade, Promega) and rotated end-over-end overnight (12 h ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were prepared in 1 × Reporter lysis buffer (Promega, E2510), and Luciferase assays were performed according to the manufacturer’s protocol (Promega ...
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bioRxiv - Neuroscience 2022Quote: ... using GoTaq® Probe 1-Step RT-qPCR System (Promega). The CDC SARS-CoV-2 nucleoprotein N2 primer set was used for amplification ...
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bioRxiv - Biophysics 2022Quote: ... and labelled with 1 µM TMR-HaloTag ligand (Promega, G8251) and 0.12 µM SiR-SNAP-Tag ligand (NEB ...
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bioRxiv - Microbiology 2022Quote: ... first with 1 µg r-LysC Mass Spec Grade (Promega) for 4 h at 30°C and then samples were diluted below 2 M urea with 100 mM Tris HCl pH 8.5 and 1 µg Sequencing Grade Modified Trypsin was added for the second digestion overnight at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... Sample B was treated with 1 µg/ml trypsin (Promega). Sample C was pre-treated with 1% Triton X-100 (EMD-Millipore ...
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bioRxiv - Immunology 2022Quote: ... Proteins were digested with 20:1 protein:trypsin (Trypsin Gold, Promega) in 50 mM ammonium bicarbonate for 3 hours at 47 °C before adding an additional 1 µg of trypsin and digesting for a further 1 hour at 47 °C ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM dithiothreitol and 1U/µl RNasin ribonuclease inhibitor (Promega) in nuclease-free water ...
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bioRxiv - Bioengineering 2022Quote: ... and then with trypsin (V5111, Promega, WI, USA; 1:100). The samples were then dried in a vacuum centrifuge for 1.5h at RT and then resuspended in running buffer ...
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bioRxiv - Immunology 2022Quote: ... Goat anti-rabbit IgG conjugated to HRP (1:5,000) (Promega) was used to detect the bound protein ...
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bioRxiv - Neuroscience 2021Quote: ... incubated with mouse anti–βIII-tubulin (1:2000; Promega, G7121) overnight at 4ºC followed by incubation with secondary antibody (donkey anti-mouse Alexa-Fluor 488 ...
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bioRxiv - Molecular Biology 2021Quote: ... Appropriate HRP-conjugated secondary antibodies were used (1:10000 Promega). Chemiluminescent signals were detected and digitally analyzed ...