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Citations for Promega :
3551 - 3600 of 3826 citations for Mouse IL 13 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2024Quote: ... The amplicon target was amplified from WNV cDNA using the qPCR primers with the forward primer flanked by T7 sequence (5’-TAATACGACTCACTATAGGGATTCGGGAGGAGACGTGGTA-3’) and transcribed using T7 RiboMAX Express Large Scale RNA Production System kit (Promega, France). RNA was purified by ethanol precipitation ...
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bioRxiv - Biochemistry 2024Quote: ... eloB and eloC) were purified on an agarose gel using the Wizard ® SV gel and PCR clean-up system purification kit (Promega). After restriction with the corresponding restriction enzymes (New England Biolabs ...
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Sphingosine induction of the Pseudomonas aeruginosa hemolytic phospholipase C/sphingomyelinase, PlcHbioRxiv - Microbiology 2023Quote: We observed induction of the chromosomal plcH-nLuc-plcR reporter in nanoluciferase reporter assays using the Nano-Glo® Luciferase Assay kit (Promega). The assay was conducted as described by the manufacturer with some modifications reported here ...
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bioRxiv - Microbiology 2023Quote: ... Nucleic Acid Purification with Proteinase K digestion was performed using Maxwell ® RSC Total Viral Nucleic Acid Purification Kit according to the manufacturer’s protocols (Promega, USA). For preparation of bacterial genomic DNA ...
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bioRxiv - Genomics 2023Quote: ... cells were washed once with cold 1× PBS and the luciferase activities were measured with Perkin Elmer Envision using Promega Dual Luciferase Assay kit (Promega, E1960). The firefly luciferase activity was normalized to renilla luciferase activity for each well ...
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bioRxiv - Genomics 2023Quote: ... The lysates were generated by adding 100 µL 1X Passive Lysis Buffer included in the Dual-luciferases assay kit (Promega E1960) to the protoplasts and vigorously shaking at room temperature for 15 minutes ...
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bioRxiv - Microbiology 2023Quote: ... The amplified PCR fragments were checked for uniformity and size using 0.8% agarose gel electrophoresis and purified using a PCR fragment recovery kit (Promega, WI, USA). The recovered DNA fragments were sequenced using the MiSeq 500PE v.2 kit (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: Genomic DNA was prepared from single cell suspensions of thymic leukemias using the Wizard Genomic DNA Purification Kit (Promega, Madison WI). PCR amplification of the Jak3 pseudokinase domain was performed using MyTaq HS Red Master Mix (FroggaBio ...
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bioRxiv - Cancer Biology 2023Quote: ... cytotoxicity and apoptosis were measured 48h and 7 days after the knock-down using ApoTox-Glo triplex assay kit (Promega,G6320) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... Amplified ORFs were then used as templates in a PCR reaction to amplify a full-length mutant amplicon followed by their purification using Wizard SV Gel and PCR Clean-Up kit (Promega, USA). These ORFs were digested with KpnI and BamHI enzymes and ligated into similarly digested pDsRed vector ...
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bioRxiv - Bioengineering 2023Quote: The viability of EBs and organoids were measured with a luminescence-based CellTiter-Glo 3D® cell viability assay kit (G9681, Promega) by following the manufacturers’ recommended protocol ...
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bioRxiv - Immunology 2024Quote: ... supernatant was collected from the replicon-transfected cells and assayed for Renilla luciferase activity according to kit instructions (Promega, cat. E2810).
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bioRxiv - Microbiology 2023Quote: ... Cell viability was assessed continuously based on ATP production with the RealTime-GloTM MT Cell Viability Assay kit (Promega, cat# G9711) through bioluminescence output using the TECAN Spark plate reader.
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bioRxiv - Genomics 2023Quote: ... We added 200 µL of PBS to each cell pool and performed the 22 DNA extractions using the Buccal Swab protocol of the ReliaPrep™ gDNA Tissue Miniprep System kit (Promega). We adjusted the lysis time to two hours before performing the RNAse treatment ...
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bioRxiv - Physiology 2024Quote: Total RNA was isolated from snap frozen LV wall or kidney after mechanical grinding (Precellys Evolution, Bertin Technologies, France) using the Maxwell RSC microRNA Tissue kit (Promega, USA). RNA was quantified via NanoDrop (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: ... Larvae from progenies with low homing rates of dsxFGFP-null were pooled to perform genomic DNA extraction (Wizard® Genomic DNA Purification Kit, Promega) and subsequently do amplicon sequencing on the dsx target to check for the presence of mutations that could have prevented the homing ...
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bioRxiv - Immunology 2024Quote: ... Total RNA was extracted from 10 ⨯ 10 µm FFPE tissue sections using the Maxwell RSC FFPE RNA kit in accordance with the manufacturer’s manual (Promega, United States). RNA concentration was determined using a Qubit 3.0 Fluorometer using the Qubit RNA BR Assay Kit (Thermo Fischer Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... The BSP amplification product was purified by Vazyme Biotech (China) amplification product purification kit and cloned into pGEM-Tasy-vector system (Promega, USA). and the percentages of methylated cytosines over total cytosines were calculated.
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bioRxiv - Molecular Biology 2024Quote: ... ATRX 5ʹ-TGAAACTTCATTTTCAACCAAATGCTC-3ʹ and 5ʹ-ATCAAGGGGATGGCAGCAG-3ʹ All PCR reactions were performed using GoTaq® G2 DNA polymerase kit from Promega following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The homogenate was centrifuged at 16,000 x g for 10 min and the supernatant was collected and diluted to 1:200 with deiodinized water for ATP measurement using the Cell Titer-Glo luminescent cell viability assay kit (Promega, G7571) and 1:10 for determining the protein concentration ...
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bioRxiv - Synthetic Biology 2024Quote: ... Luciferase activity was assayed in an Omega luminescence plate-reader (Fluostar) with four different gains following the instructions of the Nano Dual-Luciferase® Reporter kit (Promega). A further correction for luminescence bleed-through and background signal was applied using the software developed by Mauriet al.58 Then NLuc signal was divided by the FLuc signal to normalize for the transformation efficiency.
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bioRxiv - Microbiology 2024Quote: ... Cells were processed at different times post inoculation (pi) according to manufacturer’s instructions (Caspase-Glo® 3/7 Assay kit, Promega, USA) to determine caspase 3/7 activity ...
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bioRxiv - Microbiology 2024Quote: ... and tryptone (Becton Dickinson DifcoTM,)) overnight at 35°C and genomic DNA was extracted using the Wizard® Genomic DNA purification kit (Promega) as previously described [35] ...
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bioRxiv - Microbiology 2024Quote: DNA extraction of the bacterial strains isolated from oysters and cultivated on agar plates was carried with the Wizard® Genomic DNA Purification Kit (Promega) according to the manufacturer instructions ...
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bioRxiv - Plant Biology 2020Quote: ... the band of interest excised from the gel and cleaned using Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit and cloned into pGEM-T (Promega) for amplification before sequencing.
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bioRxiv - Cancer Biology 2021Quote: ... genomic DNA was extracted from cells at the indicated time points using the Maxwell® RSC Cultured Cells DNA Kit (Promega AS1620). DNA was amplified using Q5® High-Fidelity 2X Master Mix (New England BioLabs® M0492L ...
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bioRxiv - Cell Biology 2020Quote: Genomic GNA (gDNA) was extracted from each of the GFP+ and GFP-cell types using the Wizard Genomic DNA Purification Kit (Promega, Southampton, UK) and quantified using a Nanodrop 1000 Spectrophotometer (Thermo Fischer Scientific ...
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bioRxiv - Microbiology 2021Quote: ... the total genomic DNA from single colonies of isolated bacteria from the custom designed selective medium described in the section 2.3 was conducted using a Wizard™ genomic DNA extraction kit (Promega Corporation, USA), as per the manufacturer instructions ...
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bioRxiv - Immunology 2021Quote: ... and 500 ng/ml ionomycin for 6h then lysed in passive lysis buffer commercially provided in the dual luciferase reporter assay kit (Promega, no. E1910). Assay was conducted according to manufacturer’s instructions and luminescence measurements were done using CLARIOstar (BMG ...
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA extraction was performed as described for whole-genomic DNA purification using the Promega™ Wizard™ Genomic DNA Kit (A1125, Promega). Genome quality and quantity were checked in 1% agarose gels stained with %0.0001 SYBR Safe and measured in a NanoDrop (ACTGene ASP-3700 ...
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bioRxiv - Immunology 2022Quote: The ATP levels were measured according to the manufacturer’s instructions of the CellTiter-Glo® 2.0 Assay kit (Promega, Cat. No. G9241). Briefly ...
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bioRxiv - Immunology 2021Quote: ... vRNA was isolated from plasma samples using the Maxwell Viral Total Nucleic Acid Purification kit on the Maxwell 48RSC instrument (Promega, Madison WI). vRNA was then quantified using a highly sensitive qRT-PCR assay based on the one developed by Cline et al.99 ...
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bioRxiv - Immunology 2019Quote: ... was added to each well and cells were incubated for 72 hr before proliferation was measured using CellTiter 96 Aqueous One cell proliferation assay kit from Promega (Madison, WI). The data represent the average of 5 different mice immunized and treated with IFNα–C ...
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bioRxiv - Microbiology 2019Quote: ... quantified with Qubit dsDNA HS Assay kit and then cloned using the pGEM®-T Easy Vector Systems (Promega, Madison, Wisconsin, US). Plasmids were isolated using the NucleoSpin Plasmid kit (Macherey-Nagel ...
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bioRxiv - Genomics 2019Quote: ... Chile) and Drosophila americana (strain H5 from Mississipi, United States of America) with the Wizard® Genomic DNA Purification Kit (Promega Corporation). We used the consensus sequences from each satDNA identified by RepeatExplorer/TAREAN for primer’s design ...
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bioRxiv - Genetics 2019Quote: ... The longer band containing exon14b was then excised from the gel and purified using Zymoclean gel DNA recovery kit (Zymoresearch, Irvine, CA) and cloned using pGEM-T Easy Vector System (Promega, Madison, WI). The plasmid was used in a transformation into Subcloning Efficiency DH5α Competent Cells (Invitrogen ...
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bioRxiv - Synthetic Biology 2019Quote: ... The RNA was treated with Promega RQ1 DNase and reverse transcribed into cDNA using the Improm-II Reverse Transcription kit (Promega; Fitchberg, WI). Steady-state transcript levels were then measured using the cDNA template in quantitative PCR (qPCR ...
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bioRxiv - Physiology 2019Quote: ... Luciferase activity in BMDMs was determined by the tissue luciferase assay using the Bradford Luciferase Reporter Assay kit (Promega, Madison, WI, USA) following the manufacturer’s instructions as described in our previous report (25) ...
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bioRxiv - Cell Biology 2019Quote: ... gondii gDNA was prepared from freshly egressed parasites and extracted using the Wizard Genomic DNA Purification kit (Cat# A1120, Promega, Madison, WI).
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bioRxiv - Cell Biology 2019Quote: ... Ann Arbor, MI) (Fox et al., 2009; Huynh and Carruthers, 2009) using the Wizard Genomic DNA Purification Kit (Cat# A1120, Promega, Madison, WI) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: Total RNA was extracted using goat tissues from gastrointestinal tract (including abomasum, duodenum, jejunum, ileum, and caecum) using Eastep Super Total RNA Extraction Kit (Promega, Shanghai, China). cDNA was generated via PrimerScriptTM RT Reagent Kit with genomic DNA Eraser (Perfect Real Time ...
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bioRxiv - Immunology 2019Quote: ... The identity of the Huh7 cells in this study were verified using the GenePrint STR Kit (Promega, Duke University DNA Analysis Facility). The 293T and Vero (CCL-3216 and CCL-81 ...
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bioRxiv - Cell Biology 2019Quote: Viability of expanded clonal pulp cells and the parental pulp cells was measured using a CellTiter 96®Aqueous Non-Radioactive Cell Proliferation Assay Kit (MTS assay, Promega, USA) and differentiation of the cells were carried out as following.
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bioRxiv - Evolutionary Biology 2019Quote: ... Raw PCR products were then used to generate digoxigenin-labeled RNA probes using a T7 RNA in vitro transcription kit (Promega / Life Technologies). RNA was ethanol precipitated and resuspended in water to analyze on a Nanodrop ...
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bioRxiv - Evolutionary Biology 2021Quote: Genomic DNA was extracted from about 20 mg of muscle tissue using the Wizard® Genomic DNA Purification kit (Promega, WI, USA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted from concentrated phage samples using a modified protocol with the Wizard DNA Clean-Up Kit (Product #A7280; Promega, Madison, WI). Briefly ...
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bioRxiv - Neuroscience 2020Quote: The toxicity of PX was investigated on monolayers of BLEC seeded on 96 well plates or on inserts luminal side in co-culture experiments using the commercially available Cytotoxicity Detection Kit (Promega, United States). An aliquot of 25 μl medium was taken to quantify the lactate dehydrogenase (LDH ...
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bioRxiv - Cancer Biology 2020Quote: ... the full-length fragment of human PFKFB3-5 was amplified with primers PFKFB3-5 reverse and PFKFB3-5 forward and subcloned into the plasmid pGEM-T using the T/A Cloning Kit (Promega, Mannheim, Germany). Subsequently ...
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bioRxiv - Cell Biology 2021Quote: ... Cell death was determined according to the activity of lactate dehydrogenase (LDH) in the culture supernatants using a CytoTox® Kit (Promega, USA) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2021Quote: ... total RNA was extracted from ∼25 mg of powdered right brain hemisphere tissue using Maxwell 16 Tissue LEV Total RNA Purification Kit (AS1220, Promega, Madison, WI). UNC HTSF core performed RNA concentration and quality check using fluorometry (Qubit 2.0 Fluorometer ...