Labshake search
Citations for Promega :
3551 - 3600 of 3802 citations for Mouse CXCL9 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2020Quote: ... the band of interest excised from the gel and cleaned using Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit and cloned into pGEM-T (Promega) for amplification before sequencing.
-
bioRxiv - Cancer Biology 2021Quote: ... genomic DNA was extracted from cells at the indicated time points using the Maxwell® RSC Cultured Cells DNA Kit (Promega AS1620). DNA was amplified using Q5® High-Fidelity 2X Master Mix (New England BioLabs® M0492L ...
-
bioRxiv - Cell Biology 2020Quote: Genomic GNA (gDNA) was extracted from each of the GFP+ and GFP-cell types using the Wizard Genomic DNA Purification Kit (Promega, Southampton, UK) and quantified using a Nanodrop 1000 Spectrophotometer (Thermo Fischer Scientific ...
-
bioRxiv - Microbiology 2021Quote: ... the total genomic DNA from single colonies of isolated bacteria from the custom designed selective medium described in the section 2.3 was conducted using a Wizard™ genomic DNA extraction kit (Promega Corporation, USA), as per the manufacturer instructions ...
-
bioRxiv - Immunology 2021Quote: ... and 500 ng/ml ionomycin for 6h then lysed in passive lysis buffer commercially provided in the dual luciferase reporter assay kit (Promega, no. E1910). Assay was conducted according to manufacturer’s instructions and luminescence measurements were done using CLARIOstar (BMG ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... DNA extraction was performed as described for whole-genomic DNA purification using the Promega™ Wizard™ Genomic DNA Kit (A1125, Promega). Genome quality and quantity were checked in 1% agarose gels stained with %0.0001 SYBR Safe and measured in a NanoDrop (ACTGene ASP-3700 ...
-
bioRxiv - Immunology 2022Quote: The ATP levels were measured according to the manufacturer’s instructions of the CellTiter-Glo® 2.0 Assay kit (Promega, Cat. No. G9241). Briefly ...
-
bioRxiv - Immunology 2021Quote: ... vRNA was isolated from plasma samples using the Maxwell Viral Total Nucleic Acid Purification kit on the Maxwell 48RSC instrument (Promega, Madison WI). vRNA was then quantified using a highly sensitive qRT-PCR assay based on the one developed by Cline et al.99 ...
-
bioRxiv - Immunology 2019Quote: ... was added to each well and cells were incubated for 72 hr before proliferation was measured using CellTiter 96 Aqueous One cell proliferation assay kit from Promega (Madison, WI). The data represent the average of 5 different mice immunized and treated with IFNα–C ...
-
bioRxiv - Microbiology 2019Quote: ... quantified with Qubit dsDNA HS Assay kit and then cloned using the pGEM®-T Easy Vector Systems (Promega, Madison, Wisconsin, US). Plasmids were isolated using the NucleoSpin Plasmid kit (Macherey-Nagel ...
-
bioRxiv - Genomics 2019Quote: ... Chile) and Drosophila americana (strain H5 from Mississipi, United States of America) with the Wizard® Genomic DNA Purification Kit (Promega Corporation). We used the consensus sequences from each satDNA identified by RepeatExplorer/TAREAN for primer’s design ...
-
bioRxiv - Genetics 2019Quote: ... The longer band containing exon14b was then excised from the gel and purified using Zymoclean gel DNA recovery kit (Zymoresearch, Irvine, CA) and cloned using pGEM-T Easy Vector System (Promega, Madison, WI). The plasmid was used in a transformation into Subcloning Efficiency DH5α Competent Cells (Invitrogen ...
-
bioRxiv - Synthetic Biology 2019Quote: ... The RNA was treated with Promega RQ1 DNase and reverse transcribed into cDNA using the Improm-II Reverse Transcription kit (Promega; Fitchberg, WI). Steady-state transcript levels were then measured using the cDNA template in quantitative PCR (qPCR ...
-
bioRxiv - Physiology 2019Quote: ... Luciferase activity in BMDMs was determined by the tissue luciferase assay using the Bradford Luciferase Reporter Assay kit (Promega, Madison, WI, USA) following the manufacturer’s instructions as described in our previous report (25) ...
-
bioRxiv - Cell Biology 2019Quote: ... gondii gDNA was prepared from freshly egressed parasites and extracted using the Wizard Genomic DNA Purification kit (Cat# A1120, Promega, Madison, WI).
-
bioRxiv - Cell Biology 2019Quote: ... Ann Arbor, MI) (Fox et al., 2009; Huynh and Carruthers, 2009) using the Wizard Genomic DNA Purification Kit (Cat# A1120, Promega, Madison, WI) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2020Quote: Total RNA was extracted using goat tissues from gastrointestinal tract (including abomasum, duodenum, jejunum, ileum, and caecum) using Eastep Super Total RNA Extraction Kit (Promega, Shanghai, China). cDNA was generated via PrimerScriptTM RT Reagent Kit with genomic DNA Eraser (Perfect Real Time ...
-
bioRxiv - Immunology 2019Quote: ... The identity of the Huh7 cells in this study were verified using the GenePrint STR Kit (Promega, Duke University DNA Analysis Facility). The 293T and Vero (CCL-3216 and CCL-81 ...
-
bioRxiv - Cell Biology 2019Quote: Viability of expanded clonal pulp cells and the parental pulp cells was measured using a CellTiter 96®Aqueous Non-Radioactive Cell Proliferation Assay Kit (MTS assay, Promega, USA) and differentiation of the cells were carried out as following.
-
bioRxiv - Evolutionary Biology 2019Quote: ... Raw PCR products were then used to generate digoxigenin-labeled RNA probes using a T7 RNA in vitro transcription kit (Promega / Life Technologies). RNA was ethanol precipitated and resuspended in water to analyze on a Nanodrop ...
-
bioRxiv - Evolutionary Biology 2021Quote: Genomic DNA was extracted from about 20 mg of muscle tissue using the Wizard® Genomic DNA Purification kit (Promega, WI, USA) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: DNA was extracted from concentrated phage samples using a modified protocol with the Wizard DNA Clean-Up Kit (Product #A7280; Promega, Madison, WI). Briefly ...
-
bioRxiv - Neuroscience 2020Quote: The toxicity of PX was investigated on monolayers of BLEC seeded on 96 well plates or on inserts luminal side in co-culture experiments using the commercially available Cytotoxicity Detection Kit (Promega, United States). An aliquot of 25 μl medium was taken to quantify the lactate dehydrogenase (LDH ...
-
bioRxiv - Cancer Biology 2020Quote: ... the full-length fragment of human PFKFB3-5 was amplified with primers PFKFB3-5 reverse and PFKFB3-5 forward and subcloned into the plasmid pGEM-T using the T/A Cloning Kit (Promega, Mannheim, Germany). Subsequently ...
-
bioRxiv - Cell Biology 2021Quote: ... Cell death was determined according to the activity of lactate dehydrogenase (LDH) in the culture supernatants using a CytoTox® Kit (Promega, USA) according to the manufacturer’s instructions.
-
bioRxiv - Genetics 2021Quote: ... total RNA was extracted from ∼25 mg of powdered right brain hemisphere tissue using Maxwell 16 Tissue LEV Total RNA Purification Kit (AS1220, Promega, Madison, WI). UNC HTSF core performed RNA concentration and quality check using fluorometry (Qubit 2.0 Fluorometer ...
-
bioRxiv - Microbiology 2020Quote: Total RNA was extracted from 100 μl culture supernatants or apical washes using Monarch® Total RNA Miniprep Kit (Promega, Cat#: T2010S). For viral copy number quantification droplet digital PCR technology was applied (Bio-Rad Laboratories Inc ...
-
bioRxiv - Plant Biology 2021Quote: ... The PCR product was run on 2% agarose gel and the expected bands were eluted using Wizard® SV Gel and PCR cleanup kit (Promega, USA),cloned in pGEM-T easy vector (Promega ...
-
bioRxiv - Microbiology 2020Quote: ... DNA was extracted from faecal samples not stored in RNAlater® using the Maxwell 16 LEV Blood DNA kit (AS1290, Promega, UK) and automated DNA extraction system ...
-
bioRxiv - Microbiology 2021Quote: ... genomic DNA was extracted from an overnight culture of PS1793 using a Promega Maxwell Cell DNA Purification Kit (Promega Corp., Madison, WI). For short-read sequencing ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... plantarum genomic DNA was isolated via lysozyme-based cell lysis (47) followed by purification using the Wizard® Genomic DNA Purification Kit according to the manufacturer’s instructions (Promega, Dübendorf, Switzerland). The genome of the reference strain NZ3400* was sequenced at the Functional Genomics Center Zurich (Zürich ...
-
bioRxiv - Microbiology 2021Quote: DNA extraction of samples was performed according to an adapted version of the “DNA purification from human feces using the Maxwell® RSC instrument and the Maxwell® RSC PureFood GMO and Authentication Kit” developed by Promega (Promega Corporation ...
-
bioRxiv - Microbiology 2021Quote: RNA was extracted from 50–100 mg of EDTA blood or 20 mg of liver using the SimplyRNA Tissue kit and Maxwell RSC16 instrument (Promega, Wisconsin, USA), as per the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... 5% CO2 after transfections and after 48 hours were lysed using passive lysis buffer and assayed for Fluc and Rluc activity using a dual luciferase assay kit (Promega, Madison, USA) (13).
-
bioRxiv - Microbiology 2020Quote: ... High quality whole genome was extracted from a pure culture of 16h growth in Luria-Bertani broth (Oxoid, Basingstoke, UK) by using the Wizard Genomic DNA Purification Kit (Promega, Madison, USA) according to the instructions of the manufacturer ...
-
bioRxiv - Cell Biology 2021Quote: ... Cell death of infected cardiomyocytes was determined according to the activity of lactate dehydrogenase (LDH) in the culture supernatants using a CytoTox® Kit (Promega, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... PAO1-WT and few colonies that grew in 3 mM 22D-chelated condition were isolated and gDNA extracted using Wizard® Genomic DNA Purification Kit (Promega, USA) for use in whole genome sequencing ...
-
bioRxiv - Neuroscience 2022Quote: ... The firefly and Renilla luciferase activity of the cells was quantified by a Dual Luciferase Reporter System Kit (E1910, Promega, WI, USA) following the manufacturer’s instructions.
-
bioRxiv - Genomics 2022Quote: ... All growth was collected using sterile plastic loops and placed directly into Wizard Genomic DNA purification kit Nuclei Lysis solution (Promega, reference A1120). The Wizard kit extraction protocol was then followed ...
-
bioRxiv - Microbiology 2020Quote: ... Filters were transferred to 2 mL microfuge tubes for further processing using the Maxwell® RSC PureFood GMO and Authentication Kit (Promega Corporation) with a modified version of the kit protocol ...
-
bioRxiv - Immunology 2020Quote: MTS assays of HCoV-229E experiments were performed using the The CellTiter 96® AQueous One Solution Cell Proliferation Assay kit (Promega, #G3582). In brief ...
-
bioRxiv - Microbiology 2020Quote: ... Genomic DNA was extracted from 1.5□mL of that culture by using a Wizard genomic DNA purification kit (Promega, Madison, WI, USA), prepped using the Nextera XT kit ...
-
bioRxiv - Microbiology 2019Quote: ... The entire volume was extracted using a Promega Maxwell RSC Whole Blood DNA Kit on the Promega Maxwell RSC 48 (Promega, Madison, WI). Accurate DNA concentration was measured by Qubit fluorometer double-stranded DNA high sensitivity (dsDNA HS ...
-
bioRxiv - Molecular Biology 2021Quote: ... and cleaned-up PCR product concentrations were measured using a QuantiFluor® dsDNA System assay kit on a Quantus™ Fluorometer (Promega). DNA library preparation was performed using the Ligation sequencing kit SQK-LSK309 (Oxford Nanopore Technologies ...
-
bioRxiv - Microbiology 2021Quote: ... PA) and AccuMap™ low pH protein digestion kit (with trypsin and lysC) and chymotrypsin (sequencing grade) were from Promega (Madison, WI). PreScisson protease was from GenScript (Piscataway ...
-
bioRxiv - Neuroscience 2021Quote: ... Cytotoxicity was determined according to the activity of lactate dehydrogenase (LDH) in the culture supernatants using a CytoTox® Kit (Promega, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: TdT-mediated dUTP nick-end labeling (TUNEL) or EdU assays were performed by using in situ cell death detection kit (TUNEL, Promega Corporation, G3250) or EdU Click-iT® Assay Kit obtained from Thermo Fisher (C10639) ...
-
bioRxiv - Microbiology 2020Quote: ... Positive clones were cultured in liquid LB medium supplemented with kanamycin (50 μg mL−1) for extraction of plasmid DNA with the Wizard Plus SV Minipreps DNA Purification System Kit (Promega - Madison, USA). Plasmids with unique EcoRI/HindIII restriction patterns were subjected to re-transformation and phenotypic confirmation by streaking the clones in solid LB medium supplemented with kanamycin (50 μg mL−1) ...
-
bioRxiv - Microbiology 2021Quote: ... baumannii strain AYE (ATCC BAA-1710) was then similarly amplified from genomic DNA prepared with the Wizard Genomic DNA Extraction Kit (Promega, Madison, WI) using primers “F AdeC” and “R AdeC” and cloned downstream from the vector pLac site and Shine-Delgarno sequence in pBMTL-2NTC using vector primers ...
-
bioRxiv - Pathology 2022Quote: ... or 300 uL urine+DMSO using the Maxwell RSC Viral Total Nucleic Acid Purification Kit on the Maxwell 48 RSC instrument (Promega, Madison, WI).