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Citations for Promega :
301 - 350 of 680 citations for Recombinant Human Interleukin 7 Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Caspase activity was quantified using the Caspase-Glo 3/7 Assay Kit (Promega).
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bioRxiv - Molecular Biology 2023Quote: ... Cell viability and caspase 3/7 activity was measured with CellTiter Flo (Promega) or CaspaseGlo (Promega ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability and caspase 3/7 activity was measured with CellTiter Glo (Promega) or Caspase Glo (Promega ...
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bioRxiv - Bioengineering 2023Quote: ... the Caspase-Glo 3/7® or Caspase-Glo 8® assay (Promega) was performed according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... and apoptosis was determined using the Caspase 3/7 Glo Assay (G8090, Promega) measuring luminescence with a BioTek Synergy 2 (BioTek/Agilent ...
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bioRxiv - Cancer Biology 2023Quote: ... or the Caspase Glo® 3/7 Assay (Promega Corporation, Madison, WI, USA) following the manufacturer’s protocol and plates were read on an EnVision Multilabel Plate Reader (Perkin Elmer ...
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bioRxiv - Cancer Biology 2024Quote: Apo-ONE® Homogeneous Caspase-3/7 Assay (Promega Corporation, Madison, WI, USA) was used to determine the caspase activity in the extracted tumors ...
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bioRxiv - Developmental Biology 2019Quote: ... we produced recombinant H3 mutant proteins from mRNAs using rabbit reticulocyte lysate (Promega L4600). After 3h of incubation at 4°C in interphase extracts followed by another 3h incubation with anti-HA beads ...
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bioRxiv - Developmental Biology 2020Quote: ... and 200 ng purified recombinant mouse DLX2 protein in Gel Shift Binding Buffer (Promega). Unlabeled probe was added for ‘cold’ competition ...
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bioRxiv - Cell Biology 2022Quote: ... Nuclei were resuspended in reaction buffer [RNase inhibitor (0.2U/μL Recombinant RNAsin (Promega, PAN2515), 1% Fatty-acid-free BSA in PBS (Proliant ...
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bioRxiv - Biochemistry 2024Quote: ... The HaloTag-JF503-SWSAP1 was quantified by using recombinant HALO-GFP protein standard (Promega) pre-incubated with JF503 at a 1:1 molar ratio and titrated into single molecule buffer (20 mM Tris pH7.5 ...
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bioRxiv - Microbiology 2024Quote: Recombinant FIKK kinase domains activity was measured using the ADP-Glo kinase assay (Promega), which quantifies the amount of ADP produced during the kinase reaction ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 ng/μl of mRNA and 6 ng/μl QuantiLum Recombinant Luciferase protein (Promega) were coinjected ...
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bioRxiv - Plant Biology 2019Quote: ... HA- and GST-tagged proteins were synthesized in vitro using the TNT® Coupled Wheat Germ Extract Systems (Promega). For the pull-down assays ...
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bioRxiv - Immunology 2020Quote: ... The following day cells were transfected with plasmids encoding for FLAG-tagged NLRP1 (2 μg) or the indicated DPP9 construct (2 µg) with FuGENE HD according to manufacturer’s instructions (Promega). Cells were harvested ...
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bioRxiv - Immunology 2020Quote: ... The following day the cells were transfected with plasmids encoding the indicated FLAG-tagged protein (2 μg) with FuGENE HD according to manufacturer’s instructions (Promega). After 48 h cells were harvested and washed 3 times with PBS ...
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bioRxiv - Immunology 2020Quote: ... The following day cells were transfected with plasmids encoding for FLAG-tagged NLRP1 (2 μg) with FuGENE HD according to manufacturer’s instructions (Promega). After 48 h ...
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bioRxiv - Molecular Biology 2023Quote: ... Ubiquitin enrichment was performed using Halo-tagged MultiDsk TUBE (Wilson et al., 2012) preincubated with HaloLinkTM resin (G1913, Promega) for 1 hour with rotation at RT in binding buffer (100mM Tris (pH 7.5) ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293T cells were transfected with 3.5μg of N-terminally FLAG-tagged CXCR2/CXCR3/CXCR7 and 3.5μg of F22 (Promega, Cat. no: E2301). 14-16h post-transfection ...
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bioRxiv - Molecular Biology 2022Quote: ... supernatant was collected and purification was done via His-tag using HisLink Protein Purification Resin (V8823, Promega) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Cell-free translated His-VqmA was produced using the S30 T7 High-Yield Protein Expression System (Promega) according to the manufacturer’s protocol with the following amendments ...
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bioRxiv - Plant Biology 2024Quote: ... The soluble GST and His fusion proteins were purified using the MagneGST Protein Purification System (Promega, USA) or the MagneHis Protein Purification System (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... These cells were transfected in 70% confluent 6-wells TC-treated plates with 2 µg TGFβ receptor-Nanoluciferase constructs (constructs were provided by Promega and Promega R&D) using a 1 µg DNA ...
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bioRxiv - Cell Biology 2020Quote: ... Caspase 3/7 activity was measured using an Apolive-Glo kit (Promega, Madison, WI) and measured using a SpectraMax M5 (Molecular Devices ...
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bioRxiv - Physiology 2020Quote: ... caspase 3/7 activity was measured using ApoLive-Glo Multiplex Assay (Promega, Madison, WI). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... Cell apoptosis was assessed using either the CaspaseGlo 3/7 assay (G8090, Promega, UK) according to the manufacturer’s instructions (N=4 replicates) ...
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bioRxiv - Cancer Biology 2022Quote: ... After 7 days of culturing 100 μl of CellTiter-Glo 3D (Promega Cat# G9681) was added to each well and incubated for 5 minutes on a shaker ...
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bioRxiv - Evolutionary Biology 2022Quote: Effector caspase activity was assessed using the Caspase-Glo 3/7 Assay System (Promega) following a previous protocol (Ronai et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... an equal volume of Caspase-Glo® 3/7 Assay System (Cat. No.G8090, Promega) was transferred into each well and incubated for 1 h and measured by a GloMax® 20/20 Luminometer ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and caspase-3/7 activity were measured using the ApoTox-Glo Triplex Assay (Promega) in a GloMax-Multi+ Reader (Promega) ...
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bioRxiv - Molecular Biology 2019Quote: ... gDNA was isolated after 7 days using Wizard® Genomic DNA Purification Kit (Promega) for preparation of 16S Metagenomic Sequencing Library.
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bioRxiv - Molecular Biology 2021Quote: ... probes listed in Supplementary Table 7 were cloned in pGEM®-T Easy (Promega) for T7 polymerase transcription.
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bioRxiv - Cancer Biology 2021Quote: ... Apoptotic activity was determined by the Caspase-Glo 3/7 luminescent assay (Promega, G8092), Annexin-V staining (Roche ...
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bioRxiv - Bioengineering 2020Quote: ... Cell apoptosis was measured using a Caspase-Glo 3/7 assay (Promega, Madison, WI).
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bioRxiv - Cancer Biology 2023Quote: ... survival was assessed using Cell Titer-Glo and Caspase 3/7 Glo assays (Promega) and analyzed at 24–48h post-treatments ...
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bioRxiv - Cell Biology 2023Quote: Caspase activity was assessed using Caspase-3/-7 Glo Luminescent Assay (Promega, Madison, WI) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Apoptosis was assessed using the luminescence-base Caspase-Glo 3/7 assay kit (Promega) and defined by at least a 1.5-fold increase in signal activation concerning controls ...
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bioRxiv - Neuroscience 2023Quote: ... the Caspase-Glo® 3/7 assay was performed according to manufacturer’s instructions (Promega) on HEK cells after 40 h treatment with cisplatin ...
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Class IIa HDACs inhibit cell death pathways and protect muscle integrity in response to lipotoxicitybioRxiv - Cell Biology 2023Quote: ... caspase 3 activity was measured using Caspase-Glo 3/7 assay (Promega, Alexandria, Australia). Quantification of viable cells was performed by staining with 7-aminoactinomycin D (7-AAD ...
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bioRxiv - Immunology 2023Quote: ... and an apoptosis assay using Caspase-Glo® 3/7 reagent (G8091, Promega, Canada), as per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... biological replicates (n=7) were digested with Lys-C (Alpha Laboratories) and trypsin (Promega) on beads as previously described (Hubner et al. ...
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bioRxiv - Biochemistry 2019Quote: ... Dimeric constructs are based on VY208 and were created by artificially dimerization through an N-terminal GST-tag (Reck-Peterson et al, 2006) and tagged with a HaloTag (Promega) at the C-terminus as well as a GFP at the very N-terminus ...
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bioRxiv - Biophysics 2019Quote: ... the same DNA was combined with a PCR fragment amplified from cZP1full/pGEM57 to generate a 6His-tagged full-length cZP1 ORF in pSI (Promega). The pHLsec3 construct expressing C-terminally 6His-tagged mZP1-N1M1-A141 was generated by PCR ...
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bioRxiv - Cell Biology 2021Quote: ... and Flag-tagged HGF WT and HGF 4Cys-4Ala were in-vitro translated (TNT quick coupled Transcription/Translation system, Promega) and were incubated with the bead bound c-MET for 4h at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Halo-tagged ubiquitin-binding domains (UBDs) of TUBE (tandem-repeated ubiquitin-binding entities) was incubated with HaloLink resin (200 μL, Promega) in binding buffer (50 mm Tris⍰HCl ...
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bioRxiv - Genomics 2020Quote: ... HCT116 Tet-OsTIR1 Scc1-mAID-Halo cells were fluorescently tagged by incubating with 50 nM HaloTag diAcFAM (Promega, cat# G8272) or 500 nM HaloTag JF488 for 30 min ...
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bioRxiv - Synthetic Biology 2021Quote: Absolute protein concentration of HiBiT-tagged proteins was measured using the Nano-Glo® HiBiT Extracellular Detection System N2420 (Promega). CFPS reactions were diluted 104-106 -fold using 1X PLB+PI buffer (generated by mixing 2 mL of 5x Passive Lysis Buffer E1941 (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... 200 ng of DNase treated RNA library was added to 100 nM of Halo-tagged proteins immobilized onto magnetic resin (Promega). The volume of each binding reaction was 100µl in SEQRS buffer containing 200 ng yeast tRNA competitor and 0.1 units of RNase inhibitor (Promega) ...
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bioRxiv - Biochemistry 2020Quote: ... cells were transfected with empty pRK5 vector or pRK5 expressing HA-UB (HA-tagged ubiquitin) by using Fugene 6 (Promega), as per the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: Myc- and FLAG-tagged versions of the different ALOG proteins were produced using the Quick Coupled Transcription/Translation System (TnT; Promega). 25 µL of TnT reactions producing indicated proteins were mixed with Buffer 1 to reach 150 µL and rotated at 4 °C during 1 h ...