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Citations for Promega :
301 - 350 of 1313 citations for Recombinant HBV X Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The proteins were digested overnight with trypsin (Promega) at 37°C ...
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bioRxiv - Plant Biology 2021Quote: ... The pSPUTK promoter allowed in vitro protein synthesis using the TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... 20µg of chromatin was subjected to buffer exchange through SP3 protein clean up protocol (87) and resuspended in AmBic-10mM DTT before proteins digestion with 1:50 trypsin (Promega V5280) for 18h ...
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bioRxiv - Systems Biology 2024Quote: ... and an aliquot of approximately 25 µg of protein was digested with Trypsin (Promega, Trypsin to protein ratio of 1:50) at 37°C overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... PCR amplification was performed in 25 μl reactions using 5 μl X Green GoTaq Flexi Buffer (Promega), 1.5 μl MgCl2 (25mM ...
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bioRxiv - Cell Biology 2020Quote: ... 20 flies per group were homogenized on ice in a specialised buffer (1.5% Triton X-100 (Promega), complete mini EDTA-free proteinase inhibitor (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... in DEPC-treated phosphate buffer (PB) with 0.5% Triton X-100 supplemented with RNasin (Promega, Madison, WI). Sections were rinsed 3 × 10 min with DEPC-treated PB ...
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bioRxiv - Genomics 2020Quote: ... each of the wells containing 0.5 μl of lysis solution (0.2% Triton X-100 [Roche], 0.8 units of RNasin Plus [Promega] ...
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bioRxiv - Developmental Biology 2024Quote: ... and transferred into 0.2 ml PCR tubes containing 2μl of lysis buffer (0.7% Triton X-100, 2U/μl RNasin (Promega), 1μl of oligo-dT30VN primer (10 μM 5’-aagcagtggttatcaacgcagagtact30vn-3’ ...
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bioRxiv - Microbiology 2024Quote: ... diluted 10 x into sterile water and processed with the Nano-Glo® Luciferase Assay System (Promega). The bioluminescence was measured using a Spark multimode microplate reader (Tecan).
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bioRxiv - Cancer Biology 2022Quote: ... Each sample was then diluted 10-fold with 50 mM ammonium bicarbonate for digestion with 0.375 µg (for 15 µg protein samples) or 0.125 µg (for 5 µg protein samples) sequencing grade modified porcine trypsin (Promega, Madison, WI, USA). The samples were incubated at 45°C for 1 hour in a chilled microwave (CEM ...
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bioRxiv - Biochemistry 2021Quote: ... the sample was diluted 1:1 with 50 mM ammonium bicarbonate and proteins were digested with trypsin (protein to enzyme ratio 1:50, Promega, Mannheim, Germany) overnight at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... In-solution digestion was performed with LysC-Trypsin mix (1:100 enzyme: protein ratio) and trypsin (Promega; 1:50 enzyme: protein ratio). Peptides were desalted on C18 stage tips ...
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bioRxiv - Cancer Biology 2021Quote: ... The proteins were digested with trypsin (Promega Corporation, USA) at an enzyme-to-substrate ratio of 1:50 (w/w ...
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bioRxiv - Molecular Biology 2021Quote: ... 30 μg of protein were digested with trypsin (Promega) in two steps (2 h at 37 ºC with 1:40 trypsin:protein ratio ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was then digested with Trypsin Gold (Promega) 1:100 at 37 °C overnight in 10 mM ammonium bicarbonate pH 8.0 and the resultant peptides were cleaned up with POROS 20 R2 (Applied Biosystens) ...
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bioRxiv - Immunology 2019Quote: ... Protein material was digested with 600 ng Trypsin (Promega) overnight at 37°C at 300 rpm ...
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bioRxiv - Developmental Biology 2019Quote: ... Precipitated proteins were subjected to on-bead trypsin (Promega) digestion ...
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bioRxiv - Bioengineering 2021Quote: ... Proteins were digested with sequencing grade modified trypsin (Promega) at an enzyme to substrate ratio of 1:50 overnight at RT ...
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bioRxiv - Bioengineering 2020Quote: ... Proteins were digested with sequencing grade modified trypsin (Promega) at an enzyme to substrate ratio of 1:50 overnight at RT ...
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bioRxiv - Cell Biology 2021Quote: ... with that of the Halo 7-tag protein (Promega), with the insertion of a 63-base linker (21 amino acids ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were digested overnight with LysC/Trypsin mix (Promega) and sequencing-grade modified Trypsin (Promega ...
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bioRxiv - Neuroscience 2019Quote: ... Protein was digested with sequencing grade modified trypsin (Promega) using the FASP (Filter Aided Sample Preparation ...
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bioRxiv - Microbiology 2019Quote: ... proteins were predigested with 1.5 µg of rLysC (Promega) for 3 h at room temperature and samples diluted with 50 mM ammonium bicarbonate ...
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bioRxiv - Molecular Biology 2021Quote: ... with Broad Range Protein Molecular Weight Markers (V8491, Promega) used as molecular markers ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were harvested in passive lysis buffer 1x (Promega) at indicated times and
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bioRxiv - Plant Biology 2021Quote: Eluted proteins were digested with sequencing-grade trypsin (Promega) and analyzed by nano LCMS/MS on a QExactive + mass spectrometer coupled to an EASY-nanoLC-1000 (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were digested with trypsin (Promega, Madison, Wisconsin, USA) in 40 mM AmBic at 37°C for 18 h (enzyme to protein ratio (v/w ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were digested first by Lys- C (Promega, USA) at RT for 2 hours ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were digested using 0.08 μg Trypsin Gold (Promega) per gel slice at 37°C for 16 h and peptides were extracted from the band pieces using acetonitrile ...
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bioRxiv - Biochemistry 2020Quote: ... proteins were trypsinized overnight (Sequencing Grade Modified Trypsin, Promega, cleaves C-terminal to lysine and arginine residues ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were digested on beads with Trypsin/LysC (Promega) overnight followed by zip-tip cleanup using C-18 Omix tips (Agilent) ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were digested on beads with Trypsin/LysC (Promega) overnight followed by zip-tip cleanup using C-18 Omix tips (Agilent) ...
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bioRxiv - Synthetic Biology 2021Quote: ... A standard curve of HiBiT Control Protein N3010 (Promega) was diluted in PLB+PI at concentration ranging from 0.01 to 2 nM ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were detected with ECL Western Blotting Substrate (Promega) or SuperSignal™ West Femto Maximum Sensitivity Substrate (ThermoFisher ...
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bioRxiv - Plant Biology 2020Quote: ... GST-Halo was used as a protein standard (Promega). Incubate 1 μL of reticulocyte extract before binding with beads and 2 μL of extract after the binding was used for each western on a precast gel (4–20% Criterion™ TGX™ Precast Midi Protein Gel ...
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bioRxiv - Physiology 2019Quote: ... Proteins were co-precipitated with 1 µg trypsin (Promega) overnight at −20 °C in 1 ml methanol ...
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bioRxiv - Plant Biology 2020Quote: Eluted proteins were digested with sequencing-grade trypsin (Promega) and analysed by nanoLC-MS/MS on a QExactive+ mass spectrometer coupled to an EASY-nanoLC-1000 (Thermo-Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: Eluted proteins were digested with sequencing-grade trypsin (Promega) and analyzed by nanoLC-MS/MS on a QExactive+ mass spectrometer coupled to an EASY-nanoLC-1000 (Thermo-Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were digested with 300ng trypsin (Trypsin Gold, Promega) in 3µl 50mM ammonium bicarbonate followed by incubation at 37°C overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins were digested with 0.25µg of Trypsin/LysC (Promega) at a 1:100 enzyme/substrate ratio overnight at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: Eluted proteins were digested with sequencing-grade trypsin (Promega) and analyzed by nanoLC-MS/MS ...
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bioRxiv - Cell Biology 2022Quote: ... Complexed proteins were eluted in SDS Elution Buffer (Promega) for 30 minutes at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... proteins were digested with 1) chymotrypsin (Promega, Madison, USA), 2 ...
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bioRxiv - Molecular Biology 2022Quote: ... and proteins were in-gel digested with trypsin (Promega), five fractions per sample ...
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bioRxiv - Microbiology 2023Quote: ... enriched proteins were eluted by adding 1µg trypsin (Promega) and incubation for 30min at 30°C ...
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bioRxiv - Neuroscience 2023Quote: ... then with trypsin (Promega, 1:50 (protease to protein)) for 6 hours on a 37 °C shaker.
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were digested first by Lys-C (Promega, USA) at RT for 2 hours ...
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bioRxiv - Synthetic Biology 2023Quote: ... using the HiBiT Control Protein (20 µM, N3010, Promega) as a standard.
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bioRxiv - Microbiology 2023Quote: ... Proteins were digested using sequencing grade modified trypsin (Promega) with a ratio of 1:50 (enzyme:protein ...