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Citations for Promega :
301 - 350 of 6609 citations for Rat Segment polarity protein dishevelled homolog DVL 1 DVL1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were digested with trypsin (V5111, Promega). Peptide concentrations were determined using PierceTM BCA Protein Assay Kit ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were digested with LysC (Promega #VA1170) at 1:30 (enzyme:protein ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were then digested with trypsin (Promega) overnight at 37°C with an enzyme trypsin ratio of 1:50 ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were digested using trypsin (V5111, Promega) at a 1:20 (enzyme to protein ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were then digested with trypsin (Promega) - 1:100 enzyme-to- protein ratio at 37°C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... G protein chimera (Gsx) and Glo22F (Promega) using Lipofectamine 2000 (ThermoFisher ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were digested using Trypsin Gold (Promega) in 100 mM HEPES pH 7.6 at 37°C overnight ...
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bioRxiv - Systems Biology 2023Quote: ... Denatured proteins were digested with chymotrypsin (Promega) at 1:100 (enzyme:substrate(w:w) ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were digested with Trypsin (Promega, UK) overnight at 37 °C at an enzyme to protein ratio of 1:20 ...
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bioRxiv - Plant Biology 2023Quote: ... and tryptic digestion of proteins (V5111; Promega, Madison ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were pre-digested with LysC (Promega) for 2 h at 25 °C and digested with trypsin (Promega ...
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bioRxiv - Plant Biology 2024Quote: ... or the MagneHis Protein Purification System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Subsequently proteins were digested with trypsin (Promega) at 37°C for 4 h ...
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bioRxiv - Biochemistry 2023Quote: ... and the proteins were tryptic digested (Promega) at 37°C overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were digested with trypsin gold (Promega) at 1:50 enzyme to protein ratio in 50 mM TEAB ...
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bioRxiv - Cancer Biology 2023Quote: ... viable cells were quantified in a FLUOstar OPTIMA ELISA reader (550/590 nm) about 2 h after CellTiter-Blue staining (Promega, Cat No. G8081). Mean values +/-standard deviations (SD ...
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bioRxiv - Immunology 2020Quote: ... and cDNA synthesized from 1 µg total RNA (High Capacity cDNA Reverse Transcription Kit, Promega). Quantitative RT-PCR was performed using FluoCycle II SYBR Master Mix (EuroClone ...
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bioRxiv - Neuroscience 2024Quote: ... using BRYT Green Dye contained in the GoTaq 1-Step RT-qPCR kit (Promega: A6020) in the CFX96 Touch Real-Time PCR Detection system (Bio-Rad) ...
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bioRxiv - Neuroscience 2019Quote: ... The U6 reporter construct was assembled by ligating a 663bp rat U6 sequence into the HindIII and NheI sites of the pGL4.23 vector (Promega) upsteam of the minimal promoter in this vector ...
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bioRxiv - Cancer Biology 2019Quote: ... rat or cynomolgus ICOS as target cells were co-incubated with ADCC reporter cells expressing human FcγRIIIa (V158; Promega) at a 5:1 ratio ...
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bioRxiv - Microbiology 2020Quote: The interactions between host protein and RHDV nonstructural proteins were evaluated using a CheckMate Mammalian Two-Hybrid System (Promega). The proteins expressed from the pACT vector recombinant plasmid acted as prey proteins ...
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bioRxiv - Microbiology 2021Quote: ... Pull down of Halo-PfSR1 interacting proteins was performed using the HaloTag® Protein Purification System (Promega, cat # G1913) according to the manufacturer guidelines ...
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bioRxiv - Microbiology 2022Quote: ... the Halo-BysR protein was expressed using the TNT SP6 Wheat Germ Protein Expression System (Promega, Fitchburg, WI, USA). The Halo Tag-BysR protein was purified using magnetic HaloTag beads (Promega ...
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bioRxiv - Plant Biology 2023Quote: ... the PhDEF and PhGLO1 proteins were produced with the TnT SP6 High-Yield Wheat Germ Protein Expression System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were expressed using TNT® SP6 High-Yield Wheat Germ Protein Expression System (Promega, Madison, Wisconsin, United States) with 2 µg Plasmid DNA as input ...
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bioRxiv - Plant Biology 2020Quote: ... and RPM1 CC-HA (1-156aa) were expressed in vitro using the TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega; Madison, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... each sample was hydrolyzed to 100 µg of protein in a 50: 1 ratio using trypsin (trypsin Gold, MassSpec Grade, Promega, Fitchburg, WI, USA). After enzymolysis at 37 for 14 h ...
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bioRxiv - Microbiology 2021Quote: ... proteins were expressed by in vitro transcription/translation using the TNT SP6 High-Yield Wheat Germ Protein Expression System (Promega) prior to incubation with the affinity resins ...
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bioRxiv - Biochemistry 2020Quote: The two Munc13-Halo proteins were labeled by incubating the protein with Alexa 488 conjugated with HaloTag® from Promega, as described before (2) ...
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bioRxiv - Synthetic Biology 2021Quote: Absolute protein concentration of HiBiT-tagged proteins was measured using the Nano-Glo® HiBiT Extracellular Detection System N2420 (Promega). CFPS reactions were diluted 104-106 -fold using 1X PLB+PI buffer (generated by mixing 2 mL of 5x Passive Lysis Buffer E1941 (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... Antibodies were obtained in carrier/ protein-free buffer or were purified using the Magne Protein A or G Beads (Promega) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... yeast (Saccharomyces cerevisiae) cell protein extract (Cat# V7341, V7461), and human K562 cell protein extract (Cat# V6951, V6941) were purchased from Promega Inc (WI ...
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bioRxiv - Plant Biology 2022Quote: ... EMF2 and POT1a proteins) or a myc-tag (pGBKT7-DEST; TRB proteins) using a TNT Quick Coupled Transcription/Translation system (Promega) in 50 μl reaction volumes according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... These vectors were used for in vitro protein production using SP6 High-Yield Wheat Germ Protein Expression System (Promega L3260) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... EIL1-GST and GST proteins were synthesized using the TnT SP6 High-Yield Wheat Germ Protein Expression System (Promega, L3260), according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Total DNA was isolated from 1 ml culture using the Wizard Genomic DNA Purification Kit (Promega). Concentration and quality of the extracted DNA was assessed using the NanoDrop™ (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... 1 µg of RNA was used to create cDNA with the ImpromII Reverse Transcriptase Kit (Promega) following the manufactures standard protocol including oligodT oligos and 4.8 M MgCl2 in each 20 µl reaction (Promega) ...
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bioRxiv - Developmental Biology 2022Quote: ... first-strand cDNA was synthesized using 1 µg total RNA with MMLV Reverse Transcription Kit (Promega) and poly-T primer ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was quantified by real-time RT-qPCR (GoTaq 1 step RT-qPCR kit, Promega). Quantification was provided by serial dilutions of an appropriate T7-generated synthetic RNA standard ...
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bioRxiv - Microbiology 2022Quote: Viral RNA was quantified by real-time RT-qPCR (GoTaq 1 step RT-qPCR kit, Promega). Quantification was provided by serial dilutions of an appropriate T7-generated synthetic RNA standard ...
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bioRxiv - Microbiology 2023Quote: ... viral RNA was quantified by real-time RT-qPCR (GoTaq 1 step RT-qPCR kit, Promega). Quantification was provided by serial dilutions of an appropriate RNA standard ...
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bioRxiv - Microbiology 2023Quote: ... Complimentary DNA was prepared from 1 µg of RNA using a Reverse Transcription Systems Kit (Promega). Quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2023Quote: RT-qPCR was performed using the GoTaq 1-Step RT-qPCR kit (Promega Cat. No. A6020) using nifH and 16S rRNA (reference gene ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative Realtime PCR (qRT-PCR) was performed using GoTaq® 1-Step RT-qPCR kit (Promega) with primers specific for PEX1 (RE7039-RE7040 ...
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bioRxiv - Zoology 2024Quote: One-step RT-qPCR was conducted using GoTaq 1-Step RT-qPCR System kit (Promega, France) in total reaction volume of 20 µl containing 2 µl of RNA extracts and 300 nM of the same forward and reverse primers as above ...
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bioRxiv - Microbiology 2024Quote: ... Viral RNA was quantified by real-time RT-qPCR (GoTaq 1 step RT-qPCR kit, Promega). Quantification was provided by serial dilutions of an appropriate T7-generated synthetic RNA standard ...
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bioRxiv - Neuroscience 2021Quote: ... of miR-3594-5p was amplified from rat brain cDNA and cloned downstream of the Renilla luciferase reporter gene into the psiCHECK-2 vector (Promega).
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bioRxiv - Evolutionary Biology 2020Quote: ... and the orthologous 208 bp blind mole rat sequence (nanGal1 assembly; KL203377:255659-255866) into a luciferase pGL4.23[luc2/minP] vector (Promega, US). We cloned the insert in the same orientation with respect to the minP promoter and luciferase gene as the CNE and the Tdrd7 promoter are oriented in the genome ...
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bioRxiv - Biochemistry 2022Quote: ... The half site ARE reporter is derived from the minimal rat probasin sequence (Zhang et al., 2000) and was cloned into pGL3 via KpnI and NcoI restriction sites (Promega). pRL-TK (Promega ...
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bioRxiv - Neuroscience 2021Quote: The Cacnb2 3’UTR (Ensemble ID: ENSG00000165995) was amplified from a rat brain cDNA library and cloned into the pmirGLO dual-luciferase expression vector (Promega) using the PfuPlus! DNA Polymerase (Roboklon) ...