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Citations for Promega :
301 - 350 of 3874 citations for Mouse Chitinase 1 CHIT1 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: Pulldowns with cellular extracts were carried out as described for GFP-immunoprecipitations using 1-2 μg of GST-fused proteins bound to glutathione magnetic beads (Promega). For immunoprecipitations of recombinant proteins ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were diluted with ddH2O 1:5 and proteins were digested for 20 h at 37°C using trypsin (Trypsin Gold, Promega) at an enzyme/protein ratio of 1:50 ...
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bioRxiv - Cell Biology 2022Quote: ... The trapped proteins were washed four times with the methanol TEAB buffer and then digested using 1 µg Trypsin (Promega) overnight at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... The sample was diluted 1:7 with 0.1 M NH4HCO3 before overnight digestion of proteins with trypsin (Promega, cat#V5113) at 30°C ...
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bioRxiv - Cell Biology 2021Quote: ... v/v) and dehydration in ACN, proteins were digested overnight at 37 °C with trypsin (1:50, w/w) (V5280, Promega). Peptides were extracted from the gel in 50% ACN/0.1% formic acid ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were digested either with trypsin in a trypsin/protein ratio of 1/50 (w/w) (Promega Cat. No. V5111) and incubated overnight at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were diluted 8x in 50 mM ammonium bicarbonate to reduce the urea concentration to 1 M and protein digestion was performed overnight at 37 C by addition of 2 µg of trypsin (Promega) per sample.
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bioRxiv - Plant Biology 2022Quote: ... were reduced with DTT and then alkylated with iodoacetamide and digested overnight using Lys-C/Trypsin (1:50, enzyme to protein; Promega). After terminating the digestion with 1% trifluoroacetic acid ...
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bioRxiv - Microbiology 2023Quote: ... the samples were digested by trypsin with 1/80 (w/w) trypsin/total protein ratio (Sequencing Grade Modified Trypsin, Promega) according to the manufacturer recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... Alkylated proteins were then cleaved using a two-step digestion: first with Endoproteinase Lys-C (ratio 1:33 enzyme: lysate, Promega) for 1 hour at 37 °C then with Trypsin (ratio 1:33 enzyme ...
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bioRxiv - Biochemistry 2024Quote: ... at a 1:75 enzyme to protein ratio for 6 h at 30 °C and then by trypsin (V5111, Promega) (1:50 enzyme to protein ratio ...
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bioRxiv - Cancer Biology 2024Quote: ... Denatured proteins were enzymatically digested for 14 h at 37 °C with 1 µg of sequencing-grade Trypsin (V511A, Promega). After speed-vaccum drying ...
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bioRxiv - Cell Biology 2022Quote: Halo-3F-SOCS1 expression was induced with doxycycline overnight (1 μg/mL) and Halo-tagged protein detected by incubation with fluorescent Halo TMR ligand (10 nM; Promega) overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... Strap binding buffer was applied to precipitate proteins on quartz and proteolysis took place during 14 hrs at 37°C with 1 µg Trypsin sequencing grade (Promega). After speed-vacuum drying of eluted peptides ...
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bioRxiv - Cell Biology 2023Quote: ... packed column (now loaded with the protein sample) 20 µL of Trypsin digestion solution (1 µg MS-grade Trypsin (Promega) in 50 mM ammonium bicarbonate ...
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bioRxiv - Biochemistry 2023Quote: ... N- glycan release was achieved after digestion with PNGase F (1 U/10 µg protein at 37 °C overnight; Promega). Released N-glycans were hydrolysed with 25 µl of 100 mM ammonium acetate at pH 5 (1 h at RT) ...
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bioRxiv - Microbiology 2024Quote: Enzymatic deglycosylation of denatured protein samples was performed with PNGase F (1 Unit/μL) obtained from Promega (Madison, WI, USA). First ...
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bioRxiv - Neuroscience 2024Quote: The HaloTag-fused proteins expressed in neurons (10 DIV) placed on coverslips were fluorescently labeled with 1 µM AF660 (Promega) in medium for 15 min at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... protein samples were then incubated with chymotrypsin at a ratio of 1:80 (enzyme to protein) for 3-4 h at RT and then trypsin (Promega) at a ratio of 1:80 (enzyme to protein ...
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bioRxiv - Cell Biology 2024Quote: ... followed by a 4-fold dilution with 50 mM HEPES buffer pH 8.5 and overnight trypsin digestion (50:1 protein : enzyme by weight, Promega V5280) at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... The estimation of protein concentration was done using BCA protein assay (Promega #PI-23222, PI-23224). Samples were diluted using 1X sample buffer (4X stock ...
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bioRxiv - Cell Biology 2022Quote: ... The estimation of protein concentration was done using BCA protein assay (Promega #PI-23222, PI-23224). Samples were diluted using 1X sample buffer (4X stock ...
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bioRxiv - Plant Biology 2023Quote: ... proteins were co-expressed using TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega) as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The estimation of protein concentration was done using BCA protein assay (Promega #PI-23222, PI-23224). Samples were diluted using 1 X sample buffer (4 X stock ...
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bioRxiv - Cancer Biology 2024Quote: ... The estimation of protein concentration was done using BCA protein assay (Promega #PI-23222, PI-23224). Samples were diluted using 1 X sample buffer (4 X stock ...
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bioRxiv - Molecular Biology 2024Quote: ... and protein-protein interactions were analyzed with NanoBRET™ Nano-Glo® Detection System kit (Promega) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: LC-MS/MS-based mono-methylated/dimethylated-peptide analyses: NADK purified from HEK293T cells was digested with Glc-C and Lys-C mixture (sequencing grade, Promega, protein: Glc-C: Lys-C = 20:1:1) at 37°C for 16 h and desalted using C18 Zip-Tips (Millipore).
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bioRxiv - Biochemistry 2021Quote: ... Protein G magnetic beads (Promega; G7471) were washed with PBS buffer and then added to the supernatant in a 1:10 volumetric ratio ...
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bioRxiv - Microbiology 2021Quote: ... Protein was digested by trypsin (Promega) at a ratio of 1:50 (trypsin:protein ...
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bioRxiv - Biophysics 2020Quote: ... Fusion Protein were purchased from Promega. Nickel superflow resin was purchased from Qiagen (Hilden ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested with trypsin (Promega) in 50 mM ABC overnight at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested with trypsin (Promega) at 37°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were digested with trypsin (Promega) to be peptides and then processed according to the manufacturer’s protocol for TMT kit ...
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bioRxiv - Synthetic Biology 2024Quote: Purified NanoLuc protein (Promega REF G9711) was diluted to 10 µM in 0.1 mg/ml BSA solution ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were digested by trypsin (Promega) over night at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... and HiBiT Control Protein (N3010, Promega). We were unable to obtain soluble protein of AtARF9 ...
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bioRxiv - Zoology 2023Quote: ... Proteins were digested with trypsin (Promega Porcine trypsin ...
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bioRxiv - Microbiology 2024Quote: ... 250µl of Protein Precipitation Solution (Promega) was added to the lysate ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were digested with trypsin (Promega) to a final substrate ...
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bioRxiv - Microbiology 2022Quote: ... washed three times (10 minutes each) with PBST before incubation for 1 h with α-Mouse IgG HRP Conjugate (Promega) secondary antibody (1:10 000 in PBST) ...
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bioRxiv - Plant Biology 2021Quote: ... antibodies before probing with horseradish peroxidase (HRP)-conjugated anti-rabbit (for H3) or anti-mouse (for anti-GFP and anti-TUB) as the secondary antibody (1:5000; Promega, Madison ...
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bioRxiv - Microbiology 2020Quote: ... used at a 1:10,000 dilution and the secondary antibody was an Anti-Mouse IgG HRP conjugate antibody (Promega, #W4021) used at a 1:10,000 dilution.
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bioRxiv - Microbiology 2020Quote: ... the plates were washed with PBS-T five times and added with a 1:5000 dilution of HRP-conjugated goat anti-mouse IgG antibody (Promega) in blocking buffer at 37 °C for 45 min ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were washed in 1×PBST and then incubated with the secondary antibody (anti-rabbit or anti-mouse IgG conjugated with horse radish peroxidase at 1:10 000, Promega). The membranes were then revealed using an ECL kit (GE Healthcare ...
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bioRxiv - Genomics 2023Quote: ... Primary antibodies were revealed using species-appropriate HRP-conjugated secondary antibodies (anti-rabbit or anti-mouse IgG (1:5000, Promega), or anti-guinea pig IgG (1:5000 ...
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bioRxiv - Bioengineering 2024Quote: ... and incubated with the following primary antibodies diluted in TBS-T overnight at 4°C: mouse anti-HaloTag (1:5,000; Promega, G9211), mouse anti-Flag M2 (1:2000 ...
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bioRxiv - Physiology 2024Quote: ... membranes were incubated at room temperature in anti-rabbit or anti-mouse IgG (Promega, Cat# W401B and W402B, 1:10,000) for 1 hour ...
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bioRxiv - Molecular Biology 2024Quote: ... Plates were then incubated overnight at 4°C with primary antibodies (mouse anti-LgBIT antibody at 1:750 dilution [Promega N7100] ...
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bioRxiv - Plant Biology 2021Quote: ... The ANAC50 protein was purified with the HaloTag® Protein Purification System (Promega, Madison, Wisconsin, United States) using 20 μL Expression reaction for each EMSA reaction. ...
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bioRxiv - Cancer Biology 2021Quote: ... 1500 μg total protein lysate was 100ul slurry of pre-cleared with Protein G agarose beads (Promega), followed by overnight incubation with AXL antibody (2ug Ab/ 100 ul lysate) ...