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Citations for Promega :
301 - 350 of 1782 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... primers were used to amplify the PCR product (fwd 5’-GCTGTFATAGGGTGGAGGTG-3’, rev 5’GCTATCAACGCCATTGTGAA-3’) using 1X GoTaq Green (Promega, Madison, WI) with a final primer concentration of 0.2uM ...
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bioRxiv - Cancer Biology 2020Quote: ... the full-length fragment of human PFKFB3-5 was amplified with primers PFKFB3-5 reverse and PFKFB3-5 forward and subcloned into the plasmid pGEM-T using the T/A Cloning Kit (Promega, Mannheim, Germany). Subsequently ...
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bioRxiv - Neuroscience 2023Quote: ... A fragment of the rat Piezo 1 cDNA was amplified with Taq Polymerase and the oligonucleotide primers 5’-GAGGAAGAGGACTACCTT and 5’-TTTACTTAGAAAACCCTACAG from bladder total RNA and cloned into the pGEM-T Easy vector (Promega, Madison, WI). Sequence was confirmed by Sanger capillary sequencing and sense and antisense RNA probes were synthesized with T7 and SP6 RNA polymerases (Roche-Sigma-Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... 5 µL of 5X GoTaq Green Master Mix (Promega), 0.125 µL of 5u/µL GoTaq G2 polymerase (Promega) ...
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bioRxiv - Microbiology 2021Quote: ... containing 5 x 104 Jurkat effector cells (Promega # G701A) and serial dilutions of indicated human mAbs ...
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bioRxiv - Systems Biology 2020Quote: 100 mM TEAB and 5 ug trypsin (Promega V5113)
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bioRxiv - Neuroscience 2021Quote: ... + 5% RNAse inhibitor (40U/ul, Promega RNAsin inhibitor N2511). Samples were then transferred to a tube for processing by our Genome Technology Access Center (GTAC ...
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bioRxiv - Neuroscience 2021Quote: ... we used 5 μL of 5X RT buffer (Promega), 5 μL of dNTPs ...
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bioRxiv - Cancer Biology 2021Quote: ... then digested with 5 ng / μl Trypsin Gold (Promega) in 9% Acetonitrile and 40 mM Ambic ...
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bioRxiv - Immunology 2021Quote: ... 5 ng of Renilla luciferase plasmid (Promega pRL-CMV), and 50 ng of Gal4-DNA binding domain-human RORγt-ligand-binding domain fusion protein plasmid per each well ...
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bioRxiv - Cell Biology 2023Quote: ... Then 5 μl of reconstituted 1xGTPase-Glo reagent (Promega) was added ...
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bioRxiv - Neuroscience 2023Quote: ... 5 μM Bright-Glo (Fluc Luciferase Assay System, Promega) was then added to the wells ...
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bioRxiv - Molecular Biology 2022Quote: ... Constructs were labeled with 5 μM Halo-TMR (Promega) in the column for 10 minutes at room temperature and unbound dyes were washed with TEV buffer at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... at 5 µM in Tracer Dilution Buffer (Promega N291B) was added to all wells ...
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bioRxiv - Genetics 2024Quote: ... 5 µL of GoTaq G2 Green Master Mix (Promega), and 200 µM of primers ...
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bioRxiv - Genomics 2023Quote: ... 5 ug sheared salmon sperm DNA (Promega, Madison, WI), 500 ng of RNA bait ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µL of HaloTag TMR ligand (Promega, 5 mM) was added to 100 µL of JetABC (2.5 µM d-o-p ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 10 µL coelenterazine h (Promega, 5 µM final concentration) was added ...
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bioRxiv - Cell Biology 2023Quote: ... 5% glycerol) with protease inhibitor mixture without EDTA (Promega), followed by the addition of 0.3% CHAPS ...
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bioRxiv - Neuroscience 2022Quote: ... 5 µl of 5X Green GoTaq Buffer (Promega, M791A), 0.2 µl of GoTaq DNA polymerase (Promega ...
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bioRxiv - Microbiology 2023Quote: ... containing 5 x 104 Jurkat effector cells (Promega # G701A) and 10 μl of pooled mouse sera from naïve or immunized mice with HCoV-OC43 N-His ...
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bioRxiv - Molecular Biology 2023Quote: ... ∼ 5 × 105 U2OS cells were transfected using FuGene6 (Promega) with 1μg of gRNA/Cas9 (px330 ...
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bioRxiv - Microbiology 2023Quote: ... A total of 5 U of recombinant RNasin (Promega) and 10 U of RQ1 RNase-free DNase (Promega ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 μL of 5X M-MLV reaction buffer (Promega), 5 μL of a mixture of 10 mM dNTPs and 200 U of M-MLV reverse-transcriptase (Promega ...
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bioRxiv - Immunology 2020Quote: ... The following day cells were transfected with plasmids encoding for FLAG-tagged NLRP1 (2 μg) or the indicated DPP9 construct (2 µg) with FuGENE HD according to manufacturer’s instructions (Promega). Cells were harvested ...
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bioRxiv - Molecular Biology 2023Quote: ... precipitated proteins were eluted by incubation of beads with Elution Buffer (2 M Urea, 50 mM Tris pH 7.5, 2 mM DTT, 20 µg/ml Trypsin Gold (Promega)) for 30 min at 37°C shaking at 1,400 rpm in the dark ...
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bioRxiv - Cell Biology 2024Quote: ... each 5 nL of injection volume contained: 70 pg of Anillin-Halo and 5 µM Janelia Fluor® 646 HaloTag® Ligand (Promega, #GA1120), or 70 pg of Anillin-3xGFP ...
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bioRxiv - Cancer Biology 2022Quote: ... Metabolic activity was measured at day 4 with CellTiter 96 (Promega) following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2022Quote: ... Then 4 µL/well of Bright-Glo Luciferase detection reagent (Promega) was added to assay plates and incubated for 5 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Then 4 μL/well of Bright-Glo luciferase detection reagent (Promega) was added to assay plates and incubated for 5 min at room temperature ...
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bioRxiv - Physiology 2021Quote: ... and overnight protein digestion using 4 μg MS-grade trypsin (Promega) at 37°C ...
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bioRxiv - Immunology 2020Quote: ... by using 4 μL of FuGENE HD transfection reagent (Promega #E2311) according manufactured protocol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Then 4 μL/well of Bright-Glo Luciferase detection reagent (Promega) was added to assay plates and incubated for 5 min at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... 100 nM dT-primer and 4 U RNase Inhibitor (Promega, N2611). Reverse transcription was performed at 42°C for 90 min after filling up to 10 μl with RT buffer mix for a final concentration of 1x superscript II buffer (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: ... Resin was then washed 4 times with Resin Wash Buffer (Promega). Complexed proteins were eluted in SDS Elution Buffer (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... and overnight ligation at 4°C (T4 DNA ligase, Promega, USA). Ligated chimeras were transformed into DH5α (ThermoScientific ...
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bioRxiv - Biochemistry 2021Quote: ... Genomic DNA was eliminated by incubating 2 μg of RNA with 2 U of RQ1 RNase-free DNase I (Promega) for 30 min at 30°C ...
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bioRxiv - Molecular Biology 2021Quote: ... FASP was carried out in two 10 kDa MWCO filters with a 50 mM iodoacetamide alkylation step and proteins were digested in 2M urea with 2% wt/wt Lys-C (Wako) for 6 h and 2% modified trypsin (Promega) for 12 h at 37°C ...
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bioRxiv - Genetics 2022Quote: ... Vectors coding for repair template DNA of roughly 2 kb were generated from Drosophila Schneider 2 cell genomic DNA by PCR using GoTaq G2 Polymerase (Promega) and primer pairs appropriate for the desired region (Supplementary Table S1) ...
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bioRxiv - Systems Biology 2020Quote: ... Reduced proteins were alkylated and trypsin-digested for 1-2 hours in trypsin solution (2 mg/ml sequencing grade porcine trypsin (Promega)/100 mM Tris/10 mM iodoacetamide ...
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bioRxiv - Microbiology 2022Quote: 293T cells were co-transfected with pEGFP-N1 and plasmids expressing SARS-CoV-2 Spike (FL) or SARS-CoV-2 Spike-Δ19 for 24 h using FugeneHD (Promega). The cells were treated with DMSO or 10 µM 6-TG at 4 h post- transfection ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were diluted to a concentration of urea of 2 mol L-1 and digested overnight with the addition of 2 µl sequencing-grade trypsin (Promega). After digestion ...
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bioRxiv - Molecular Biology 2023Quote: Proteins bound to beads were reduced with 2 mM DTT in 2 M urea buffer and sequencing grade trypsin (Promega) was added to a final concentration of 5 ng/μl ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were then digested overnight with 2 µg of lysyl endopeptidase (Wako) at room temperature and further digested overnight with 2 µg trypsin (Promega) at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... 3.5 mM MgCl2, and 2 mM ATP (for nucleic acid substrates containing RNA, 2 U/μl of RNasin Ribonuclease Inhibitor (Promega)) for 30 min at 37 °C ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... The pellet was washed with 2 ml nuclei storage buffer and resuspended in 0.2 ml PBS (calcium and magnesium-free) with 2% BSA and 0.2 U/μl RNasin® Plus RNase inhibitor (Promega). Buffer composition was described before72 ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 µl RNasin Plus (Promega, catalog no. N2615), 0.8 µl 100 mM ATP (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... using 2 µg of sequencing grade trypsin (Promega) per fraction 55 ...
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bioRxiv - Biochemistry 2022Quote: ... either 2 µg pGL4.47[luc2P/SIE/Hygro] (Promega) or pGL4.52[luc2P/STAT5RE/Hygro] (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... t-ERK1/2 (Promega V114A, 1/1000 dilution), p-AKT (Cell Signaling Technology ...