Labshake search
Citations for Promega :
3351 - 3400 of 3802 citations for Mouse VEGFR3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... Cell viability was measured using a commercially available kit [CellTiter 96 Aqueous One Solution Cell Proliferation Assay (Promega, Madison, USA)] ...
-
bioRxiv - Microbiology 2024Quote: Genomic DNA was extracted from bacterial cells grown overnight in MOKA-rich medium using the Wizard genomic DNA purification kit (Promega) or as described (62 ...
-
bioRxiv - Microbiology 2024Quote: SAM-dependant N6mA DNA methylation activity of PglX was probed in vitro using an MTase-Glo Methytransferase Assay kit (Promega). The kit allows indirect measurement of SAM-dependent methyltransferase activity via production of the SAH reaction product ...
-
bioRxiv - Molecular Biology 2024Quote: ... Macroscopic dissection of tumor areas was performed with sterile scalpels and tissues were extracted using the Maxwell RSC FFPE Plus DNA Purification Kit (Promega) according to the manufacturer’s instructions.
-
bioRxiv - Molecular Biology 2024Quote: ... In a 96 well plate (white bottom) 20 µL lysate was mixed with 50 µL Luciferase Assay Reagent (#E1501 kit, Promega) (as per the manufacturer’s instructions) ...
-
bioRxiv - Immunology 2024Quote: ... The supernatants from cells stimulated with inflammasome activators were collected and subjected to lactate dehydrogenase (LDH) assay to assess cell death using a CytoTox 96 non-radioactive cytotoxicity assay kit (Promega). Both the supernatants and cell pellets were collected for immunoblotting analyses.
-
bioRxiv - Immunology 2024Quote: Cell pellets from cells stimulated with inflammasome activators were used to measure cell viability through checking cellular ATP levels with a CellTiter-Glo luminescent cell viability assay kit (Promega). The supernatants from cells stimulated with inflammasome activators were collected and subjected to lactate dehydrogenase (LDH ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Bacterial genomic DNA was extracted from static overnight cultures of WS clones using the Wizard Genomic DNA purification kit (A1125, Promega) following the manufacturer’s instructions ...
-
bioRxiv - Genomics 2024Quote: Stereocaulon virgatum RNA was extracted from the fungal culture using the Maxwell® RSC Plant RNA Kit (Promega, Madison, WI). Tissue was homogenized in 30 second intervals for 1.5 minutes at 30hz ...
-
bioRxiv - Microbiology 2024Quote: ... Total DNA from feather pulps and tracheas were extracted using the MagaZorb® DNA mini-prep kit (Promega, Madison, WI), following the manufacturer’s recommendations with a few modiications ...
-
bioRxiv - Systems Biology 2024Quote: ... Extracts were used for quantification of luciferase as proxy for ATP content using the ENLITEN ATP Assay System Bioluminescence Detection Kit (Promega) according to the manufacturer instructions ...
-
bioRxiv - Microbiology 2024Quote: ... The bacteria were pelleted by centrifugation and DNA extraction was performed with the Wizard® genomic DNA purification kit (Promega).
-
bioRxiv - Neuroscience 2021Quote: ... Culture media was then replaced with 50 µL of qNSC/aNSC media containing 1 mM 2DG (2-deoxy-D-Glucose, provided in Glucose Uptake-Glo kit from Promega (J1342)) reagent for 10 minutes in incubator (humidified ...
-
bioRxiv - Cancer Biology 2021Quote: ... the production of IFN-γ in the supernatant was quantitated using an ELISA-ready-go! Kit (eBioscience) and cell pellets were used to measure organoid viability using a CellTiter-Glo® Luminescent Cell Viability Assay (Promega).
-
bioRxiv - Cell Biology 2020Quote: ... and SERBP1 were amplified from human cDNA and cloned into the appropriate vectors (pACT or pBIND) provided by the CheckMate Mammalian Two-Hybrid kit (Promega E2440). The appropriate combination of edited pACT and pBIND vectors ...
-
bioRxiv - Immunology 2021Quote: ... Cell supernatants were analyzed for LDH activity using the CytoTox 96® Non-Radioactive Cytotoxicity Assay Kit (Promega, Cat. no: G1780).
-
bioRxiv - Genetics 2021Quote: ... Approximately 1 μg RNA per sample was converted to cDNA using the ImProm-II Reverse Transcription System kit (Promega, Madison, WI) using the Oligo(dT)15 primer supplied with the kit and with a final concentration of 3 mM MgCl2 according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... the fluorescence derived from the incorporation of BRYT Green® Dye into the double-stranded PCR products was measured at the end of each cycle using the GoTaq® qPCR Master Mix 2X Kit (Promega). The results were analysed using Bio-Rad CFX Maestro software ...
-
bioRxiv - Microbiology 2019Quote: ... from 25 randomly selected CM samples was extracted by an automated Maxwell 16 DNA extraction platform using blood DNA purification kits (Promega, UK) following previously described protocols2 ...
-
bioRxiv - Microbiology 2019Quote: ... Viral RNA was isolated from homogenized tissues using the Maxwell 48 RSC Viral Total Nucleic Acid Purification Kit (Promega, Madison, WI) on a Maxwell 48 RSC instrument ...
-
bioRxiv - Neuroscience 2021Quote: RNA was extracted from 10 flies for each experiment condition and the process followed the protocol of Promega ReliaPrepTM RNA Tissue Miniprep System kit (Promega, Z6110). Extracted RNA was stored in a −70°C refrigerator ...
-
bioRxiv - Molecular Biology 2019Quote: ... a TUNEL assay was performed on some sections according to the protocol for the DeadEnd Fluorometric TUNEL Kit (Promega, Fitchburg, WI). Stained sections were imaged using fluorescent microscopy and TUNEL-positive cells in the outer-nuclear layer (ONL ...
-
bioRxiv - Molecular Biology 2021Quote: ... The release of lactate dehydrogenase (LDH) into the cell culture medium was quantified by bioluminescence using the LDH-Glo™ Cytotoxicity Assay kit (Promega). 10% Triton X-100 was added to untreated cells for 15 minutes to determine the maximum LDH release ...
-
bioRxiv - Biophysics 2022Quote: ... The resulting 2.6-kb fragment was separated from the 1.4-kb fragment and uncut plasmid by agarose gel purification (Promega Gel Wizzard Kit). A set of oligonucleotides that form a biotinylated and primed fork was ligated to one end of the fragment and the final product purified on a Sepharose-4B column as previously described [42] ...
-
bioRxiv - Microbiology 2022Quote: ... A total of 56 Fusobacterium isolates were recovered and subjected to DNA extraction using the Wizard genomic extraction kit (Promega, USA). For each isolate ...
-
bioRxiv - Plant Biology 2019Quote: ... 1 g of fresh leaves were collected from each genotype and DNA was extracted using the Wizard Genomic DNA Purification Kit (Promega, USA) following manufacturer instructions ...
-
bioRxiv - Plant Biology 2019Quote: Total of RNA was extracted from various tissue of plants by using the Eastep®Super Total RNA Extraction Kit (Promega). The cDNA was synthesized from Dnase I treated total RNA using M-MLV 1st Strand Kit (Invitrogen) ...
-
bioRxiv - Synthetic Biology 2019Quote: Liquid cultures of the transformed cells were used for plasmid extraction and purification using the Wizard® Plus SV Minipreps DNA Purification System kit (Promega). The plasmids quality and concentration were quantified using the Synergy HTX plate reader (BioTek ...
-
bioRxiv - Microbiology 2019Quote: ... quantified with Qubit dsDNA HS Assay kit and cloned using the pGEM®-T Easy Vector Systems (Promega, Madison, Wisconsin, US). Plasmids were isolated using the NucleoSpin Plasmid kit (Macherey-Nagel ...
-
bioRxiv - Plant Biology 2020Quote: A luciferase-based kinase activity assay was performed according to the manufacturer’s instructions of ADP-Glo™ Kinase assay kit (Promega, Germany). 1nmol kinase (cytoplasmic domain ...
-
bioRxiv - Developmental Biology 2021Quote: ... pCDNA-Cnot3 Δ701-751 and pCDNA-Cnot3Δ651-751 were in vitro transcribed/translated using a TNT® Quick Coupled Transcription/Translation kit (Promega, USA) according to manufacturer’s instructions using 10μCi of 35S-Methionine to radiolabel the proteins ...
-
bioRxiv - Genetics 2021Quote: ... of G1 and G2 mosquitoes fluorescently expressing the actin5c::eCFP construct was extracted as individual samples using the Wizard® Genomic DNA Purification Kit (Promega). Regions of gDNA were then amplified according to the three primer pairs as indicated in Fig ...
-
bioRxiv - Systems Biology 2021Quote: ... 2 ul were used as templates for PCR with the srrm3 or srrm4 primers designed to amplify the genomic region of interest (listed in Table S12) and GoTaq® Flexi DNA Polymerase kit (Promega).
-
bioRxiv - Biochemistry 2021Quote: ... liquid cultures were pelleted at 3000 g for 10 minutes and miniprepped using the Tecan Fluent robotic liquid handler with Promega Wizard SV 96 Plasmid DNA Purification Kit (Promega; A2250).
-
bioRxiv - Bioengineering 2020Quote: ... Cells were incubated with nanoparticles encapsulating Fluc mRNA and analyzed for cell viability and luciferase activity with the ONE-Glo™+Tox luciferase reporter and cell viability assay kit (Promega) using a multimode microplate reader.
-
bioRxiv - Immunology 2021Quote: Around 1×105 cells were treated as indicated prior to lysis and RNA isolation using the ReliaPrep™ RNA Miniprep kit (Promega). Approximately 1μg of total RNA was reverse transcribed into cDNA using SuperScript III (Invitrogen ...
-
bioRxiv - Biophysics 2021Quote: ... We detected the cytotoxicity by a lactate dehydrogenase (LDH) assay using the Cytotox 96 non-radioactive cytotoxicity assay kit (Promega, USA) according to the kit instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... at 3500 cells/well and cell proliferation over 3-5 day periods was determined by measuring luminescence with CellTiter-Glo® assay kit (G7570, Promega), according to manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2021Quote: We performed the apoptosis analysis of the MPro stable cells and cells transiently transfected with the pLVX-MPro-eGFP-2 plasmid using the RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay kit from Promega. The cells were maintained in high glucose DMEM medium supplemented with 10% FBS ...
-
bioRxiv - Biochemistry 2020Quote: miRNAs were isolated from ultracentifuged EVs resuspended in 25 ul of phosphate buffered saline (PBS) using the Maxwell® RSC miRNA Plasma and Serum Kit (AS1680, Promega) following the manufacturer’s instruction ...
-
bioRxiv - Microbiology 2020Quote: ... The duplicate amplification products of each sample were pooled and purified with the SV Wizard PCR Purification kit (Promega, Madison, WI) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: The cytotoxicity of compounds on HEK293T and Vero cells was evaluated using the CytoTox-Glo™ Cytotoxicity Assay kit (Promega, #G9290), according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2020Quote: ... hLamp1-mCherry (this study) were prepared using the Ambion mMessage Machine kit using Sp6 (#AM1340) supplemented with RNAse Inhibitor (Promega #N251B), and diluted to 30–80ng/μl for injection into embryos ...
-
bioRxiv - Developmental Biology 2020Quote: ... The α-tub∷GFP (Chung et al., 2014) plasmid was injected at 5-10ng/μl after purification using the PureYield Midiprep kit (Promega, #A2492).
-
bioRxiv - Zoology 2021Quote: ... The PCR products were detected through 2% agarose gel electrophoresis and purified with an AxyPrep DNA gel recovery kit (Axygen Biosciences, Union City, CA, USA) and then quantified with a QuantiFluor(tm)-ST blue fluorescence quantitative system (Promega, USA). Purified amplicons were pooled in equimolar amounts ...
-
bioRxiv - Cell Biology 2021Quote: In vitro translated (IVT) proteins were generated in rabbit reticulocyte lysate using the SP6 TNT Quick-Coupled Transcription/Translation kit (Promega, L2080) according to the manufacturer’s instructions.
-
bioRxiv - Cell Biology 2021Quote: ... PCR fragments amplified from genomic DNA extracted from the different founder mice using the Wizard SV genomic DNA extraction kit (Promega, A2360), were cloned into a vector using the TOPO TA cloning kit (Thermo Fisher Scientific).
-
bioRxiv - Cell Biology 2021Quote: ... was synthesized from 1 μg of total RNA, using oligo-dT primers (USB Corporation, Cleveland, USA) and a reverse transcriptase kit (ImPROm-II, Promega, USA), according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2022Quote: Intracellular glutamate was measured in chondrocytes in 96 well plate format utilizing Glutamate-Glo assay kit (J7021, Promega, Madison, WI USA). Luminescence was measured on microplate reader ...
-
bioRxiv - Molecular Biology 2022Quote: Total RNA was extracted from lung tissue using the Maxwell automated instrument and the Maxwell® RSC Viral Total Nucleic Acid Purification Kit (Promega). The concentration of RNA and quality were measured with HS Total RNA 15nt.methods (Agilent ...