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Citations for Promega :
3301 - 3350 of 3973 citations for Indeno 1 2 3 Cd Pyrene D12 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... Complementary DNA (cDNA) was synthesized from 1 μg of total RNA using random primers and Reverse Transcriptase System (Promega). The cDNA was used as a template for the real-time PCR amplification using PowerUp SYBR® Green Master Mix and a StepOne Plus system (Applied Biosystem ...
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bioRxiv - Immunology 2019Quote: ... 0.4 μg envelope vector (pMD2.G) and 1.6 μg hairpin-pLKO.1 vector (SHC016 control or gene specific shRNA. Fugene-6 (Promega) was used as transfection reagent ...
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bioRxiv - Plant Biology 2019Quote: ... Secondary goat anti-rabbit IgG conjugated with HRP was used at a dilution of 1:10,000 (Promega, Madison, WI).
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bioRxiv - Cancer Biology 2019Quote: ... The sample was then diluted 50% with PBS before sequencing grade trypsin (1 μg per sample, Promega Corp, V5111) was added for an overnight incubation at 37 °C ...
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bioRxiv - Synthetic Biology 2019Quote: ... The samples were diluted to 1% SDC using 100mM ammonium bicarbonate and mass spectrometry grade Trypsin (Promega, WI, USA) was added at a ratio of 1:50 (µg Trypsin:µg Protein ...
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bioRxiv - Microbiology 2021Quote: ... 1 μL of forward and reverse primer (5 μM, see Supplementary Table 1) and 5.5 μL of Nuclease-free Water (Promega). The PCR I was performed as follows ...
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bioRxiv - Immunology 2021Quote: Total RNA was extracted from sorted cells with RiboEx (GeneAll) and 0.2–1 μg was subjected to cDNA synthesis using the GoScript Reverse Transcription system (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The 1/10 of the sample was kept for injection and the 9/10 were digested with trypsin (Promega) (12.5 ng/µl ...
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bioRxiv - Plant Biology 2020Quote: ... 10 mg/L BSA and 1 mM PMSF) and assayed using the Dual-Luciferase® Reporter Assay System (Promega). Experiments were normalized to negative control set at 1.
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bioRxiv - Synthetic Biology 2021Quote: ... 1 µl of this crude lysate was used for PCR in a 10 µl GoTaq Green reaction (Promega M7123) with 0.25 µM of primers ...
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bioRxiv - Developmental Biology 2020Quote: ... Postnatal testes chemically synchronized for undifferentiated spermatogonia were homogenized at 2% w/v in polysome buffer (50 mM Tris, pH 7.4, 100 mM KCl, 12 mM MgCl2, 1% Nonidet P-40, 1mM DTT, 200 U/mL Promega RNasin ...
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bioRxiv - Biochemistry 2021Quote: ... The next day the sample was further diluted to 1 M urea and 750 ng Glu-C (Promega, V1651) were added and incubated for another 8 hours at 37°C.
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bioRxiv - Neuroscience 2021Quote: ... the supernatant was removed and the nuclei pellet was resuspended into 500ul nuclei storage buffer (NSB: 1x PBS; Invitrogen, 0.5% RNase free BSA;Serva, 1:200 RNaseIN plus inhibitor; Promega, 1x EDTA-free protease inhibitor ...
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bioRxiv - Cell Biology 2021Quote: ... membranes were subjected to three wash periods for 5 minutes in 1x TBS with 0.5% Tween and then probed with horseradish peroxidase–conjugated goat anti-mouse or goat anti-rabbit secondary antibodies at 1:2,500 (W4021, W4011, Promega) in 1x TBS with 5% nonfat dry milk and 0.5% Tween at room temperature for 1 hour ...
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ARL3 Mediates BBSome Ciliary Turnover by Promoting Its Outward Diffusion through the Transition ZonebioRxiv - Cell Biology 2021Quote: ... Five micrograms of RNA were reverse transcribed at 42 °C for 1 h using M-MLV Reverse Transcriptase (Promega) and oligo(T)18 primers (Takara) ...
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bioRxiv - Cell Biology 2021Quote: ... RPE1 cells were co-transfected with 1 µg KIF1C-mCherry-FRB and 0.5 µg FKBP-GFP-Myc-MAO using Fugene 6 (Promega) in fibronectin-coated glass-bottom dishes and imaged 24 hours later on an Olympus Deltavision microscope (Applied Precision ...
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bioRxiv - Systems Biology 2021Quote: ... Then urea concentration was diluted to 1.5 M with HPLC water and 1:50 (w/w) trypsin (Promega GmbH) was added for 12 h at 37 °C with mixing at 700 rpm ...
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bioRxiv - Plant Biology 2020Quote: ... and incubated with an anti-rabbit immunoglobulin horseradish peroxidase-conjugated secondary antibody (1:4000 dilution, lot no. 187662; Promega) to detect SOG1-Myc ...
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m7G cap-eIF4E interaction stimulates polysome formation by enhancing first-round initiation kineticsbioRxiv - Biophysics 2021Quote: ... The translation reaction mixture for single-molecule detection was assembled according to the manufacturer’s instructions with the following modifications: 1) the RNase inhibitor RNaseIn (Promega) was used at a final concentration of 0.8 U/ul ...
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bioRxiv - Microbiology 2021Quote: ... Protein was reduced with 5 mM DTT and alkylated with 14 mM iodoacetamide followed by digestion with trypsin (1:100, w:w) (Promega) as described in [80] ...
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bioRxiv - Microbiology 2021Quote: ... 0.025% deoxycholate and 200 µg.mL−1 RNase A at 37°C for 20 min to lyse the cells and Protein Precipitation Solution (Promega) was added ...
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bioRxiv - Microbiology 2021Quote: ... 0.1 % Tween 20 and incubated for 1 h with the secondary IgG (HRP-conjugated donkey anti-rabbit antibodies, Promega) diluted 1:20,000 in TBS ...
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bioRxiv - Systems Biology 2021Quote: ... Protein digestion was performed for 16 h at 37 °C with 0.1 µg of Trypsin/Lys-C mix (1 µL of stock; Promega) and stopped by lowering pH to 3 by the addition of 5 µL of 10% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: ... and the sample was digested at 37 °C with sequencing-grade trypsin (Promega; 1:50 protease:protein w/w ratio) or at 25°C with Glu-C (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... For Halo-tag labeling cells were allowed to re-attach for 1 hour under hypoxic conditions and then incubated with 0.5 uM of Janelia Fluor 549 (Promega) for 15 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... at a protein:Lys-C ratio of 100:1 (w/w) for 4 h at 37°C followed by trypsin (Promega) digestion at a ratio of 50:1 (w/w ...
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bioRxiv - Biophysics 2020Quote: ... Samples were then diluted to 1M urea by 50mM AB and digested by 1:50 (m/m) trypsin (Promega) overnight shaking at 600rpm ...
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bioRxiv - Cell Biology 2021Quote: cDNA synthesis was performed using 1-5 μg of total RNA with random hexamers and reverse transcriptase (Promega, GoScript). Quantitative RT-QPCR was performed in triplicate for each samples using the KAPA SYBR PCR kit (Sigma Aldrich KK4600 ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were then diluted with water (1:5) and incubated overnight at 37°C with 0.5 µg of sequencing grade modified trypsin (Promega). Samples were acidified using 1% TFA and peptides separated into 3 fractions on a 200 µL StageTips packed with three Empore™ SPE Disks SDB-RPS (3M ...
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bioRxiv - Cell Biology 2021Quote: ... then samples were digested overnight at 37°C on a rotator with modified trypsin (1:20 trypsin:sample ratio) (Promega). Peptides were desalted using SepPak C18 columns (Waters ...
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bioRxiv - Biophysics 2022Quote: ... and resuspended in assay buffer (HBSS with 20 mM HEPES pH 7.45) with 1 μg/mL freshly prepared coelenterazine h (Promega). Cells were plated in white-walled ...
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bioRxiv - Cancer Biology 2022Quote: The 4T1-mScarlet and 67NR-GFP cell lines were generated by transfection using a 1:4 ratio of plasmid DNA:FugeneHD reagent (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... RT) and sequentially digested with Lys-C (Wako) (protein:enzyme ratio 1:50, o/n at RT) and trypsin (Promega) (protein:enzyme ratio 1:100 ...
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bioRxiv - Biochemistry 2022Quote: ... The pellet was resuspended in 0.1M Triethylammonium bicarbonate (TEAB) and overnight (∼16 h) digestion was performed with trypsin/Lys-C mix (1:25 protease:protein; Promega) at 37° C with constant mixing using a thermomixer ...
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bioRxiv - Bioengineering 2022Quote: ... various concentrations of the compounds were mixed with 1 nM sensor and 1,000-fold diluted furimazine (Nano-Glo Luciferase Assay Substrate, Promega) in 100 μL of buffer (50 mM HEPES ...
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines were cultured in Dulbecco’s modified Eagle medium (DMEM) (HyClone) with 10% fetal bovine serum FBS (HyClone) and 1% penicillin-streptomycin (P.S.) (Promega) at 37 °C in a 5% CO2 atmosphere.
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bioRxiv - Immunology 2021Quote: ... plates were washed 5 times with wash buffer (PBS with 1% v/v bovine serum albumin (Capricorn Scientific) and 0.05% v/v Tween-20 (Promega)) ...
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bioRxiv - Developmental Biology 2020Quote: ... The cDNA was synthesized from 1 μg of the total RNA using the ImProm-II Reverse Transcription System (Promega) and oligo (dT)23 primers.
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bioRxiv - Molecular Biology 2020Quote: ... RNA-dependent interactions were assessed by treating nuclear extracts with 1 U/μl RNasin® Ribonuclease Inhibitors (Promega, # N2615) as control or 1 mg/ml of RNase A (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... Viral RNA was detected using the CDC 2019-Novel Coronavirus Real-Time RT-PCR Diagnostic Panel as per Centre for Disease and Control’s optimised protocol (Integrated DNA Technologies) for use with Go-Taq 1-step RT-qPCR Master Mix (Promega). Gene expression profile and SARS-CoV-2 N subgenomic RNA expression were determined with a standard qPCR cycle (50°C for 2 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... was used in a 1:1000 dilution and horseradish peroxidase (HRP)-conjugated goat anti-rabbit secondary IgG (W401B, Promega) was used in a 1:20,000 dilution ...
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bioRxiv - Microbiology 2021Quote: ... Samples were diluted with 0.1M ammonium bicarbonate solution to a final concentration of 1% sodium deoxycholate before digestion with trypsin (Promega) at 37°C overnight (protein to trypsin ratio ...
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bioRxiv - Biochemistry 2021Quote: ... Phospho-Tyrosine specific antibodies (anti P-Tyr674/675 from Cell Signalling 1:3000) and anti-p75 intracellular antibody (Promega) were used ...
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bioRxiv - Bioengineering 2020Quote: ... a 632 bp fragment of the 121-promoter [15] was amplified by PCR (Suppl. Table 1) and inserted between the BglII and HindIII sites of pGL4.10 (Promega) using NEBuilder HiFi DNA Assembly Master Mix.
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bioRxiv - Neuroscience 2022Quote: ... The mixture was then incubated with 1 μg of AKT substrate II peptide (CKRPRAASFAE) as the Pak4 substrate (Promega) or 1 μg of GST-Raf13 as the Pak1 substrate with a total volume of 5 μL in a 384-well plate (PerkinElmer ...
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Herpes simplex virus 1 entry glycoproteins form stable complexes prior to and during membrane fusionbioRxiv - Microbiology 2022Quote: ... the media of the effector cells was replaced with 40 µl per well of fusion medium (Ham’s F12 with 10% FBS, Penicillin/Streptomycin, 50 mM HEPES) with 1:50 Endurazine luciferase substrate (Promega) added ...
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bioRxiv - Cancer Biology 2022Quote: ... 20 minutes in the dark, RT) and sequentially digested with Lys-C (Wako) (protein:enzyme ratio 1:50, overnight at RT) and trypsin (Promega) (protein:enzyme ratio 1:100 ...
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bioRxiv - Cancer Biology 2019Quote: ... washed thrice with TBS-T and incubated for 1 hour with HRP-conjugated secondary antibody (Promega, Madison, WI, USA). After four additional washes ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Synthesis of cDNA was performed with 1 μg of total RNA by using the GoScriptTM Reverse Transcription System (Promega), according to the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2019Quote: ... and 1 μg of RNA was reverse-transcribed by the oligo (dT) primer using a reverse transcription kit (Promega) after digestion with RNase-free DNaseI (Fermentas) ...