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Citations for Promega :
3301 - 3350 of 3879 citations for 6 Bromo 2 trifluoromethylimidazo 1 2 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... CFPS reactions were run as described above with the addition of 1 μl of FluoroTect™ reagent (Promega L5001) per 50 μl CFPS reaction ...
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bioRxiv - Genetics 2022Quote: ... membranes were washed and incubated with the appropriate HRP conjugated secondary antibody (1:3,000 anti-rabbit W401B antibody from Promega), and the reaction was finally visualized with the Western Blotting Luminol Reagent (Santa Cruz Biotechnology) ...
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bioRxiv - Molecular Biology 2023Quote: ... 1/20th of the resulting cDNA was used then analyzed by qRT-PCR using GoTaq qPCR master mix (Promega). The following primer pairs were used:
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bioRxiv - Systems Biology 2023Quote: ... Digestion was performed overnight in 300 μL of 50 mM 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid (HEPES) buffer at pH 7.5 supplemented with endoproteinase Lys-C (Wako) and trypsin (Promega) at room temperature ...
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bioRxiv - Systems Biology 2023Quote: ... Samples were diluted 1:3 with 50 mM HEPES pH 8.5 and then digested by a 1:50 (trypsin to protein) ratio of sequencing grade modified trypsin (Promega) for 16 hours at 600 rpm and 25°C ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins captured on the columns were then digested by adding 20 μL of digestion buffer (50 mM TEAB, pH 8.5) containing 1 μg of sequencing grade trypsin (Promega) 1 hour at 47
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bioRxiv - Evolutionary Biology 2023Quote: ... 3 nM of either XeARPP19 or various forms of ClyARPP19 (stock solutions at 1 μg/μL) were incubated in the presence of 62.5 units of recombinant bovine PKA (Promega) and 1 mM γS-ATP in a final volume of 30 μL of PKA Buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... 100 ng of purified genomic DNA was spiked with 1 ng of unmethylated lambda DNA (Promega, Madison, WI, USA) and subjected to bisulfite treatment using the EZ DNA methylation gold kit (Zymo Research ...
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bioRxiv - Neuroscience 2023Quote: ... centrifuged and resuspended in EZ lysis buffer, followed by three washes in wash/resuspension buffer (1x PBS, 1% BSA, 0.2U/μL RNase Inhibitor (Promega)) and labeled with propidium iodide (PI ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed 3 times for 10 min in TBS-T and subsequently incubated with species-specific HRP-conjugated secondary antibodies (1:10,000; Promega) in 5% non-fat dry milk -TBS-T for 1 h at room temp ...
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bioRxiv - Genetics 2023Quote: ... samples were suspended in homogenization buffer (50mM Tris, pH7.5, 100mM KCl, 12mM MgCl2, 1%NP40-substititue, 1mM DTT, 200U/mL Promega RNasin ...
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bioRxiv - Microbiology 2023Quote: ... and 400-800 ng of end repaired gDNA using a 1:20 dCTP:ddCTP mixture (9.5 mM dCTP Millipore Sigma #3732738001, 0.5 mM ddCTP Promega #U1225) to generate ∼20 bp C-tails.
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bioRxiv - Immunology 2023Quote: ... and digested for 4 hrs with 2 mg/ml LysC (Wako Chemicals GmbH) in a ratio of 1:50 and O/N with 0.1 µg/µl trypsin (Promega) in a ratio of 1:50 ...
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bioRxiv - Biochemistry 2023Quote: ... 10 µL of 1:100 (diluted in phenyl red-free DMEM with 4% FBS) Nano-Glo substrate (Promega N1572) was added in each well ...
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bioRxiv - Developmental Biology 2023Quote: ... with 1 ug of RNA and RT-qPCR reactions were performed using GoTaq® qPCR Master Mix (A6002 - Promega). Analyses were carried out in the QuantStudio™ 5 Real-Time PCR System ...
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bioRxiv - Cancer Biology 2023Quote: ... 170 µL medium was removed and 30 µL caspase 3/7 reaction (1:100 Z-DEVD-R110 fluorogenic substrate:Homogenous buffer; ApoONE, Promega) was added to each well ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were diluted 3 fold with 20mM HEPES pH 8.0 and digested in 10 @g ml-1 trypsin-TPCK (Promega) overnight at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... The milk solution was removed and the membrane was incubated with a 1:1,000 dilution of streptavidin-alkaline phosphatase antibody (Promega) in TBS-T for 1 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were proteolysed for 2 h at room temperature using 0.5 μg Lys-C (Wako) and subsequently for 16 h at 37 °C using 1 μg trypsin (Promega). Peptides were collected by centrifugation and acidified with 0.5 % trifluoroacetic acid.
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bioRxiv - Biochemistry 2023Quote: ... Samples were initially digested for 1 hour at 47°C with sequencing grade trypsin (Promega, San Luis Obispo, CA) dissolved in 50 mM TEAB (pH 7 ...
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bioRxiv - Biophysics 2023Quote: ... In the first round of selection 400 nM of Xl-HAS-1-loaded nanodiscs diluted in the selection buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 1% BSA) was immobilized on streptavidin paramagnetic beads (Promega). Beads were washed three times in the selection buffer ...
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bioRxiv - Immunology 2023Quote: ... the cells plus supernatants were combined with an equal volume of the respective caspase-Glo reagent for 1 h according to the manufacturer’s instructions (Promega). The mixture was then transferred to a Lumitrac 600 plate and luminescence was measured using a SpectraMax i3 instrument and the SoftMax Pro 6.3 Software (Molecular Devices) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... CP4 medium was exchanged with Hanks’Balanced Salt Solution (with added glucose 1 g/l, NaHCO3 0.35 g/l) containing GloSensor cAMP Reagent (3 %, Promega) and plates were incubated for 60 min ...
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bioRxiv - Developmental Biology 2023Quote: Digoxigenin-labelled antisense riboprobes were synthesized from cloned cDNA fragments (Supplementary file 1) using T7 or SP6 polymerases (Promega) and digoxigenin-labelled dUTPs (Roche) ...
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bioRxiv - Cell Biology 2023Quote: ... urea was diluted to 1 M and proteins digested overnight with modified sequencing grade trypsin (Promega, Madison, WI, USA) at a 1:50 enzyme:protein ratio ...
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bioRxiv - Plant Biology 2023Quote: ... Two µl of cDNA (1:4 dilution) was added to a 20µl qPCR reaction using the GoTaq qPCR Master Mix (Promega) and ran on a BioRad CFX Opus96 thermocycler ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.05% (v/v) NP-40 and 0.5% (v/v) Triton X-100) containing 20 U ml−1 of RNasin (Promega), 1 mM DTT ...
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bioRxiv - Microbiology 2023Quote: ... in TBS-T followed by a washing step for 30 min with TBS-T and a final 60 min incubation with with 1:10.000 horseradish peroxide-conjugated anti-rabbit IgG (Promega) in TBS-T ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 ng pCas9 mCherry Frame +1 and 100ng pCRISPaint HaloTag-Puro donor plasmids were transfected using ViaFect (Promega E498A). Three days post-transfection cells were sub-cultured into media supplemented with puromycin (0.8 µg/mL ...
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bioRxiv - Biochemistry 2023Quote: ... Trypsin digestion was performed using a trypsin solution with a concentration of 1 μg /μL (Trypsin Gold, V528A, Promega), and 0.6 μL of the trypsin solution was added to achieve a final ratio of 1:50 ...
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bioRxiv - Biochemistry 2023Quote: Cells stably expressing epitope-tagged NK1R were transiently transfected (Lipofectamine™ 3000 Transfection Reagent) with 1 μg of pGL4.29[luc2P/CRE/Hygro] or pGL4.30[luc2P/NFAT-RE/Hygro] plasmids (Promega Corp.), seeded in a white-walled 96-well flat clear bottom plate (Corning #3610) ...
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bioRxiv - Immunology 2023Quote: ... RH5ΔNC-specific B cells were identified as live CD19+ IgG+ RH5ΔNC-APC+ RH5ΔNC-PE+ cells and single cell sorted into 96-well plates containing 10µL/well lysis buffer (10mM Tris [T3038, Merck], 1 unit/mL RNasin Ribonuclease Inhibitor [N2515, Promega]) and frozen at -80 °C.
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bioRxiv - Molecular Biology 2023Quote: ... protoplasts were transferred onto BCD-growth medium containing ammonium tartrate and geneticin G418 antibiotic (50 µg ml−1; Promega) followed by two weeks growth period without the antibiotic and second selection period for two weeks with antibiotic again.
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bioRxiv - Molecular Biology 2023Quote: RNA samples from two biological replicates were pooled and total RNA was treated with DNase (1 U/µl, Promega). For cDNA synthesis ...
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bioRxiv - Developmental Biology 2024Quote: ... containing the promoter region of spia-1 (1.23 kb upstream from the start ATG) and the entire genomic region into the pGEMT vector (Promega). A transcriptional construct (pSO22) ...
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bioRxiv - Plant Biology 2024Quote: ... The alkylation was stopped by the addition of 1 µl of 200 mM cysteine and the samples digested overnight with 1 µl of 0.5 µg/μl trypsin (MS Gold, Promega). The resulting peptides were desalted and subsequently analysed on UltiMate™ 3000 RSLCnano System connected to LTQ Orbitrap Velos mass spectrometer (Thermo Fisher Scientific).
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bioRxiv - Immunology 2024Quote: ... plates were washed 5 times with wash buffer (PBS with 1% BSA (Capricorn Scientific) and 0.05% Tween-20 (Promega)) ...
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bioRxiv - Cell Biology 2024Quote: ... reduced (6.5mM DTT, 1 hour at 60°C), alkylated (54mM Iodoacetamide, 30min, RT) and digested with 1µg of Trypsin (Promega) (96) ...
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bioRxiv - Genetics 2024Quote: ... YFP-negative first instar larvae of the progeny of gwnull- 1/PBac(681.P.FSVS-1)PMCACPTI001995 or gwnul1-2/PBac(681.P.FSVS-1)PMCACPTI001995 were collected 38 hr after egg laying and were lysed in Passive lysis buffer (Promega). After centrifugation ...
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bioRxiv - Microbiology 2024Quote: ... At 8 and 24 hpt monolayers were lysed with 100 μl 1 x passive lysis buffer according to the manufacturer’s instructions (Promega), stored at −80°C and analysed using Dual-luciferase substrate (Promega ...
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bioRxiv - Cell Biology 2024Quote: ... cells were stained with 1 nM Halo Tag Ligand (HTL) tagged with Janelia Fluor 646 (HTL-JF646, Promega, #GA1110) for 30 min at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... The sample was processed according to the SP3 protocol36 and digested overnight with trypsin (Promega, enzyme/protein 1:50). Peptides were desalted using SBD-RPS tips as previously described37 ...
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bioRxiv - Cancer Biology 2024Quote: Purified rRNA (1 to 3 µg) was digested overnight at 37 °C with 270 units of Nuclease S1 (Promega) using the supplied buffer ...
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bioRxiv - Biochemistry 2020Quote: 2.5 μg of bacmid DNA per construct obtained from amplification in DH10BacY cells were used to transfect 1 Mil Sf9 cells/per construct using Fugene transfection reagent (FuGENE HD, Promega). Virus at P0 was harvested and used to produce a stock Virus P1 solution to further infect 1 lt culture of Sf9 cells at 0.8-1 Mil/ml in SF921 medium containing Pen/Strep ...
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bioRxiv - Biochemistry 2021Quote: ... Genomic DNA was eliminated by incubating 10 μL of isolated RNA with 1 U of RQ1 RNase-free DNase I (Promega) for 30 min at 30°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were digested overnight at 37 °C with 5 μL of trypsin (1 μg dissolved in 50 mM HEPES pH 8.0, Promega V5111). The trypsin digestion was quenched by adding 4 μL of 1× EDTA-free protease inhibitor cocktail (Roche 11873580001) ...
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bioRxiv - Cell Biology 2020Quote: ... Homogenates were diluted 1/100 with the extraction buffer and mixed with the luminescent solution (CellTiter-Glo Luminescent Cell Viability Assay, Promega). Luminescence was measured with a SpectraMax Gemini XPS luminometer (Molecular Devices) ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was synthesized by reverse transcription from 1 μg total RNA using a ImProm-II™ Reverse Transcription System (Promega). Primers for NMNAT1 ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were then cleared by centrifugation and the supernatant was diluted (1/50) with extraction buffer and mixed with luminescent solution (CellTiter-Glo Luminescent Cell Viability Assay, Promega). The luminescence was measured on a Victor 3 plate reader (Perkin Elmer) ...
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bioRxiv - Developmental Biology 2021Quote: ... HEK-293T cells were co-transfected with corresponding pLKO.1 plamids and 3rd generation packaging lentiviral plasmids (pLP1, pLP2, pLP-VSVG) using FuGENE HD (Promega), according to the manufacturer’s recommendations ...