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Citations for Promega :
3251 - 3300 of 4270 citations for 7 Bromo 1 2 3 4 tetrahydronaphthalen 1 ol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... Lysates were diluted 1:10 with Milli-Q water and processed for PCR using GoTaq G2 Flexi DNA Polymerase kit (Promega). Reactions were placed in a thermocycler on a touchdown program ...
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bioRxiv - Biochemistry 2024Quote: ... then resuspended in 1 gel volume of 25 mM ABC containing 5 ng/µL of digestion grade trypsin (Promega, V5111) and incubated at 37°C for 16 hours for a full digestion or for 15 minutes for a partial digestion ...
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bioRxiv - Neuroscience 2024Quote: ... then transfected with pRK5-based constructs of iGluR subunits and Neto variants (1 µg total DNA/ ml cells) using ViaFect transfection reagent (Promega). The cells were incubated at 37°C for 3 hours ...
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bioRxiv - Plant Biology 2024Quote: ... in presence of 40 U of RNase OUT (both from Invitrogen, using ∼1 μg of total RNA and 100 ng of a mixture of random hexanucleotides (Promega) and incubated for 50 min at 50 °C ...
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bioRxiv - Biochemistry 2024Quote: ... 1 μg of total RNA was then reverse transcribed by extension of random primers using M-MLV (Promega, Madison,WI). Relative expression levels were determined by real-time PCR (qPCR ...
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bioRxiv - Immunology 2024Quote: ... Trypsin was reconstituted in 50 mM acetic acid in water at a concentration of 1 μg/μL (Trypsin Gold, Promega). Reconstituted trypsin was added to 50 mM NH4HCO3 in a 1:50 ratio (v/v) ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 min) and diluted to 1M urea with 200 mM ammonium bicarbonate for trypsin digestion (1 µg, 37°C, 8h, Promega). After digestion ...
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bioRxiv - Immunology 2024Quote: ... Quantification of cell-cell-cell fusion was performed by measuring luciferase expression as relative luminescence units (RLU) 1 min by mixing cell lysates with the Steady-Glo luciferase substrate (E2520, Promega) on a Synergy H1 plate reader (Biotek) ...
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bioRxiv - Microbiology 2024Quote: ... aliquots of cell lysates were diluted 1:30 and assessed for firefly and Renilla luciferase activity using a Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... proteins were alkylated in 5 mM iodoacetamide for 1 hour in the darkness at RT and digested overnight at 37°C with 1 µg trypsin (Trypsin Gold Mass Spectrometry Grade, Promega). Peptides were recovered by centrifugation ...
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bioRxiv - Cell Biology 2020Quote: ... the artificially synthesized PlGF 3’ untranslated regions (UTR) gene fragment was constructed into pMIR-reporter (Promega, Madison, WI, USA). A complementary sequence with mutation of the seed sequence was designed based on the wild type (WT ...
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bioRxiv - Molecular Biology 2020Quote: ... FLuc levels were measured 3-days post-AAV administration using a Luciferase 1000 Assay System kit (Promega Cat#E4550) per manufacturer’s instructions and read on a Veritas luminometer with settings ...
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bioRxiv - Genomics 2020Quote: ... 3 ug of pCAG-NLS-Bxb1 was diluted in 250 uL of OptiMEM and 6 uL of Fugene (Promega). On day 2 ...
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bioRxiv - Cell Biology 2019Quote: ... nuclei from HepG2 cells were isolated by incubating cell pellets in 500 μl of a hypotonic buffer for 15 min on ice (20 mM Tris-HCL, pH 7.4, 10 mM NaCl, 3 mM MgCL2, with protease and phosphatase inhibitor cocktail from Promega). Triton X-100 detergent was added to a 0.5% v:v final concentration to lyse the cells followed by centrifugation at 1200 × g for 10 min to pellet nuclei ...
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bioRxiv - Cell Biology 2021Quote: ... fumigatus pyrGAf gene and 870 nucleotides of the myoE 3’-UTR region was cloned in pGEM-T easy (Promega). This plasmid was used as template for site-directed mutagenesis (QuickChange kit ...
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bioRxiv - Molecular Biology 2020Quote: MC Stem-HalV and GUS-(HalV IGR) mRNAs (3 pmol) were translated in 20μl reaction volume of Flexi rabbit reticulocyte lysate (Promega) in the presence of [35S]methionine (>37.0 TBq/mmol ...
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bioRxiv - Microbiology 2020Quote: ... Cells were incubated at 37°C/5% CO2 for 3 days before performing CellTiter-Glo (CTG) assays as per the manufacturer’s instruction (Promega). Luminescence was read using a Molecular Devices Spectramax L plate reader ...
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bioRxiv - Cancer Biology 2021Quote: ... wild-type and mutant MMP2 3′-UTRs were synthesized and recombined into pmirGLO Luciferase vectors (Promega, Madison, WI, USA). Then pmirGLO-Wt/pmirGLO-Mt or miR-1299 mimics/miR-NCs were co-transfected into EC9706/KYSE30 cells ...
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bioRxiv - Genomics 2022Quote: ADGRG6_3 and ADGRG_4 sequences were PCR amplified from human genomic DNA and cloned into the pGL4.23 plasmid (Promega, E8411). Human preadipocytes ...
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bioRxiv - Immunology 2020Quote: ... pH 8.0 using Vivaspin columns (3 kDa) and digested separately O/N using trypsin or chymotrypsin (Mass Spectrometry Grade, Promega) at a ratio of 1:30 (w/w) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were incubated at 37°C and 5% CO2 for 3days before CellTiter-Glo (CTG) assays were performed as per the manufacturer’s instructions (Promega). Luminescence was read using a Molecular Devices SpectraMax L plate reader.
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bioRxiv - Molecular Biology 2019Quote: ... 200 µg lysate was mixed with 0.1 µl RNase A (~3 mg/ml) and increasing concentrations of RNasin (Promega), as indicated ...
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bioRxiv - Pathology 2021Quote: ... Cytopathic effects of the virus were measured after 3 days using the CellTiter-Glo luminescent cell viability assay (Promega), according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... The RIP fraction was washed with Washing Buffer (0.3 M NaCl; 20 mM Tris-HCl pH7.5; 5 mM MgCl2; 5 mM DTT; protease inhibitor tablet; RNasin PROMEGA) three times ...
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bioRxiv - Cell Biology 2020Quote: ... The sample was first treated by 2.5 μg of LysC (Wako) for 3 h at 37 °C with shaking and then treated with 2.5 μg of Trypsin (Promega) for over-night at 37 °C with shaking ...
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bioRxiv - Bioengineering 2021Quote: ... on a 3.5 cm glass-based dish were transfected with 1.0 μg of EBFP (plasmid DNA) using 3 μL of FuGENE HD Transfection Reagent (Promega) in 10 μL of Opti-MEM (Life Technologies Corporation) ...
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bioRxiv - Microbiology 2021Quote: ... and NS1 (3′) sequences were determined from purified product cloned into the pGEM-T vector by TA-cloning (Promega) according to the manufacturer’s guidelines.
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bioRxiv - Microbiology 2020Quote: ... pH 8.0 using Vivaspin columns (3 kDa) and digested separately overnight using trypsin or chymotrypsin (Mass Spectrometry Grade, Promega) at a ratio of 1:30 (w/w) ...
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bioRxiv - Molecular Biology 2024Quote: ... RT-PCR was used to amplify the OGDH 3’UTR and sub clone it into pmirGLO vector (Promega, UK). 5 × 10^4 C2C12 cells were seeded into 12 well plates ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were diluted 10-fold with 25 mM ammonium bicarbonate and digested with 3 µg of trypsin (Promega, v5111) overnight at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... After 3 days the cells were washed with PBS and lysed with Cell Culture Lysis Buffer (Promega cat. #E1531) prior to firefly and Renilla luciferase activity measurements which were performed in triplicate using the Dual-GLO kit (Promega cat ...
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bioRxiv - Microbiology 2023Quote: ... Imaging was performed 3 min after intraperitoneal (i.p.) injection of 150 mg/kg D-Luciferin (Beetle Luciferin Potassium Salt, Promega) in the IVIS® Spectrum In Vivo Imaging System under 2.5% isoflurane inhalation anesthesia using 15 min exposure ...
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bioRxiv - Cell Biology 2023Quote: ... and 500 ng of lenti-viral transfer plasmids previously described along with 3 µl of Fugene 6 (E2692, Promega) transfection reagent ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Probe synthesis reactions were performed at 37 °C for 3 h and then were treated with DNase I (Promega) at 37 °C for 20 min ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were seeded onto acid-washed coverslips at a density of 3 × 104 cells per coverslip for 24 h before transfection with 1μg of plasmid DNA using 3 μl Fugene HD transfection reagent (Promega). HaloTagged AP-2 σ2 was visualized by adding Janelia Fluor 646-HaloTag ligand (Promega) ...
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bioRxiv - Molecular Biology 2024Quote: ... The coding sequence of NanoLuc was amplified by PCR using the primers 5-NanoL and 3-NanoL from pNL1.1.CMV plasmid (Promega). The resulting fragment was digested with BamHI and NotI enzymes and cloned into pEGFP-N1 digested with the same enzymes ...
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bioRxiv - Molecular Biology 2024Quote: ... and transfected with 0.5 μg of “driver” plasmid + 0.5 μg of SVA “reporter” plasmid (pAD26) using 3 μL FuGENE® HD (Promega); the following day ...
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bioRxiv - Molecular Biology 2024Quote: ... HeLa cells were co-transfected with 0.5 μg of “driver” plasmid (pTMO2F3) + 0.5 μg of pAluneoTet using 3 μL FuGENE® HD (Promega); the following day ...
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bioRxiv - Biochemistry 2020Quote: ... 2 μg of plasmid was transfected using Fugene HD (Promega, Madison WI, #E2311). Plasmid DNA was mixed with 8 µL Fugene and 100 µL media and incubated at room temperature for 15 minutes before being added to cells ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested by 2 h Trypsin/Lys-C mix incubation (V5073, Promega) and collected ...
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bioRxiv - Cancer Biology 2021Quote: ... and real-time PCR using GoTaq 2-Step RT-qPCR kit (Promega, A6110). All measurements were normalized against Actin as the internal control using the 2-ΔΔCt method (Figure 1—source data 3 and Figure 3—source data 2) ...
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bioRxiv - Immunology 2021Quote: ... Luciferase activity was measured after 2 days using Nano-Glo luciferase substrate (Promega) and plates were read in an Envision 2103 microplate luminometer (PerkinElmer) ...
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bioRxiv - Biochemistry 2020Quote: ... HALO-MultiDsk (2 mg) was incubated with 200 µl of HaloLink resin (Promega) in binding buffer (50 mM Tris–HCl pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... the proteins were digested with 2 μg of trypsin/Lys-C mix (Promega) for 16 h at 37°C ...
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bioRxiv - Plant Biology 2019Quote: ... 12.50 μl of 2× PCR Green master mix (GoTaq DNA polymerase; Promega, USA) and 8.50 μl nuclease free water ...
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bioRxiv - Biochemistry 2020Quote: ... Seven other trypsins were used and are denoted as Trypsin-2 (V5111; Promega), Trypsin-3 (786-254 ...
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bioRxiv - Neuroscience 2020Quote: ... 7.5 μl of 2× SYBR Green PCR Master Mix (Promega, Madison, WI, USA) and sterile milliQ water to a final volume of 15 μl per tube ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein samples were digested by 2 µg sequencing grade trypsin (Promega, Madison, WI) at 37°C with shaking overnight ...
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bioRxiv - Plant Biology 2021Quote: ... 2 mg of total RNA were treated with RQ1 RNase-Free DNase (Promega) and processed with the ImProm-II Reverse Transcription System (Promega ...
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bioRxiv - Microbiology 2021Quote: ... Luciferase activity was measured after 2 days using Nano-Glo luciferase substrate (Promega) and plates were read in an Envision 2103 microplate luminometer (PerkinElmer) ...