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Citations for Promega :
3251 - 3300 of 4181 citations for 4' Trifluoromethoxy 5 trifluoromethyl 1 1' biphenyl 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... 1 μg of total RNA was incubated with RNase-free DNase I (1 unit/μl) following the protocol provided by the manufacturer (Promega), followed by reverse-transcription into first-strand cDNA using ImProm II reverse transcriptase (Promega) ...
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bioRxiv - Immunology 2023Quote: ... prepared by adding NanoDLR™ Stop & Glo® Substrate 1:100 into NanoDLR™ Stop & Glo® Buffer (Promega, USA), were added to the samples ...
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bioRxiv - Microbiology 2023Quote: ... The reactions were subjected to primer extension at 42°C for 45 min using 1 unit of MMLV-RT (Promega) and 0.5 mM of dNTPs ...
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bioRxiv - Molecular Biology 2023Quote: ... Culture aliquots (200 µL) were taken to measure OD620 and luminescence by adding 10 µL of 1 mg/mL beetle luciferin (Promega) in a BioTek Cytation 3 Microplate Reader ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then washed with 100 μL PBS and lysed by addition of 20 μL 1 x Passive Lysis Buffer (Promega) to each well and nutated for 15 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells were transfected with pCI-neo-hsSART1 full-length and ideletion mutants with 1 ng/μl using ViaFect Transfection Reagent (Promega). After 4 h incubation ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested with 1 μg of lysyl endopeptidase (Wako) at room temperature overnight and were further digested overnight at room temperature with 1 μg trypsin (Promega). The resulting peptides were desalted with an HLB column (Waters ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with 49 ng of a TP1-Luciferase (Kurooka et al., 1998; Minoguchi et al., 1997) and 1 ng Renilla-Luciferase (pRL-TK, Promega) using Lipofectamine™ 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting supernatant was diluted at 1:50 with PBS and used for luminescence measurement with the Nano-Glo Luciferase Assay System (cat. #N1110, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... N- glycan release was achieved after digestion with PNGase F (1 U/10 µg protein at 37 °C overnight; Promega). Released N-glycans were hydrolysed with 25 µl of 100 mM ammonium acetate at pH 5 (1 h at RT) ...
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bioRxiv - Biochemistry 2023Quote: ... The captured proteins were washed twice in 200μl 95% MeCN and transferred to 200μl 25mM ammonium bicarbonate containing 1 μg sequencing grade modified trypsin (Promega, Madison, USA) and mixed for 4 h at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... lysyl endopeptidase (Wako) at 25°C for 2 hours followed by another overnight digestion with 1:50 (w/w) trypsin (Promega) at 25°C ...
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bioRxiv - Microbiology 2023Quote: ... core and modified histones and transcription factors at the HIV-1 LTR was assessed by quantitative PCR using primers spanning the full promoter (Table 1) with GoTaq qPCR Master mix kit (Promega) in a CFX Connect Real-Time PCR thermocycler (BioRad) ...
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bioRxiv - Neuroscience 2023Quote: ... lysyl endopeptidase (Wako) at 25°C for 2 hours and further digested overnight with 1:50 (w/w) trypsin (Promega) at 25°C ...
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bioRxiv - Neuroscience 2024Quote: ... then transfected with pRK5-based constructs of iGluR subunits and Neto variants (1 µg total DNA/ ml cells) using ViaFect transfection reagent (Promega). The cells were incubated at 37°C for 3 hours ...
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bioRxiv - Plant Biology 2024Quote: ... in presence of 40 U of RNase OUT (both from Invitrogen, using ∼1 μg of total RNA and 100 ng of a mixture of random hexanucleotides (Promega) and incubated for 50 min at 50 °C ...
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bioRxiv - Microbiology 2024Quote: ... aliquots of cell lysates were diluted 1:30 and assessed for firefly and Renilla luciferase activity using a Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Quantification of cell-cell-cell fusion was performed by measuring luciferase expression as relative luminescence units (RLU) 1 min by mixing cell lysates with the Steady-Glo luciferase substrate (E2520, Promega) on a Synergy H1 plate reader (Biotek) ...
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bioRxiv - Biochemistry 2024Quote: ... 1 μg of total RNA was then reverse transcribed by extension of random primers using M-MLV (Promega, Madison,WI). Relative expression levels were determined by real-time PCR (qPCR ...
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bioRxiv - Immunology 2024Quote: ... Blots were incubated with primary antibodies overnight at 4°C followed by 2-hour incubation with HRP-conjugated secondary anti-rabbit IgG-HRP conjugate antibodies (1:10,000; Promega W401B) at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... 30 min) and diluted to 1M urea with 200 mM ammonium bicarbonate for trypsin digestion (1 µg, 37°C, 8h, Promega). After digestion ...
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bioRxiv - Microbiology 2024Quote: ... proteins were alkylated in 5 mM iodoacetamide for 1 hour in the darkness at RT and digested overnight at 37°C with 1 µg trypsin (Trypsin Gold Mass Spectrometry Grade, Promega). Peptides were recovered by centrifugation ...
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Adaptation of CD4 in gorillas and chimpanzees conveyed resistance to simian immunodeficiency virusesbioRxiv - Microbiology 2024Quote: ... Secondary antibodies were diluted in blocking buffer and incubated with the membrane for 60 min at room temperature (1:10,000 anti-rabbit-HRP, Promega #W401B). After secondary antibody incubation ...
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bioRxiv - Cancer Biology 2024Quote: ... and digested overnight at room temperature with 1 µg/µL endoproteinase Lys-C (Wako) followed by a digestion with sequencing-grade trypsin (Promega) at a final concentration of 1 ng/μL 6 h at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... For cDNA synthesis up to 1 µg of total RNA was reverse transcribed using random hexamer primers and M-MLV Reverse Transcriptase (Promega). Gene expression was measured using the primaQUANT CYBR qPCR mastermix (Steinbrenner Laborsysteme GmbH ...
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bioRxiv - Plant Biology 2024Quote: ... The complementary DNA (cDNA) synthesis was performed with 1 μg of total RNA using ImProm-II™ Reverse Transcriptase (Promega). cDNA was diluted in DNase-free water and used for qRT-PCR with Go Taq® qPCR Master Mix reagent (PROMEGA ...
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bioRxiv - Microbiology 2024Quote: ... The lysate was clarified and combined with an equal volume of a 1:50 Nano-Glo Luciferase Assay Substrate: Nano-Glo Luciferase Assay BuKer (Promega). Luminescence was read at 200 gain on the BioTek Cytation5 (Agilent Technologies).
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bioRxiv - Neuroscience 2024Quote: ... The samples were then diluted with 50mM NH4HCO3 to a final concentration of less than 2M urea and then and further digested overnight with 1:50 (w/w) trypsin (Promega) at 25°C ...
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bioRxiv - Microbiology 2024Quote: ... The reactions were subjected to primer extension at 42[°C for 45[min using 1 unit of MMLV-RT (Promega) and 0.5[mM of dNTPs ...
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bioRxiv - Physiology 2024Quote: ... membranes were incubated at room temperature in anti-rabbit or anti-mouse IgG (Promega, Cat# W401B and W402B, 1:10,000) for 1 hour ...
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bioRxiv - Plant Biology 2024Quote: ... First-strand cDNA was synthesized from half a volume of total RNA (1 μg) treated for 30 min at 37°C with RQ1 RNase-free DNase (Promega) using a SuperScript III First-Strand Synthesis System (Invitrogen) ...
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bioRxiv - Developmental Biology 2024Quote: ... Lysates were diluted 1:10 with Milli-Q water and processed for PCR using GoTaq G2 Flexi DNA Polymerase kit (Promega). Reactions were placed in a thermocycler on a touchdown program ...
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bioRxiv - Plant Biology 2024Quote: ... Reverse transcription was performed on 1 μg total RNA using an oligo (dT)18 primer and MMLV reverse transcriptase (Promega). For qPCR ...
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bioRxiv - Molecular Biology 2024Quote: ... We harvest 1 OD600 unit of yeast in mid-exponential growth phase and resuspended it in 100 μl Passive Lysis Buffer (Promega) with mixing for 2 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... the precipitated proteins were dissolved by 100 mM NH4HCO3 and digested overnight by trypsin (trypsin: protein ratio,1: 50, Promega) at 37°C ...
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bioRxiv - Microbiology 2024Quote: Enzymatic deglycosylation of denatured protein samples was performed with PNGase F (1 Unit/μL) obtained from Promega (Madison, WI, USA). First ...
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bioRxiv - Neuroscience 2024Quote: The HaloTag-fused proteins expressed in neurons (10 DIV) placed on coverslips were fluorescently labeled with 1 µM AF660 (Promega) in medium for 15 min at 37°C ...
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bioRxiv - Systems Biology 2024Quote: ... The transfected cells were treated with 5 ng/ml TGFβ1 or vehicle for 15 min and Luciferase activities were measured using the Dual-Luciferase Reporter Assay Kit (E1910; Promega).
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bioRxiv - Synthetic Biology 2024Quote: ... encoding each aaRS was incubated at 30 °C for 8 h in a customized PURE system (version 1) containing fluorescently labeled lysyl-tRNA (FluoroTect GreenLys, Promega). After expression ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 µL of an aliquot of the reaction mixture was added to 30 µL of the luciferase assay reagent (Promega), and luminescence was measured as translational activity using a luminometer (GloMax ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 µL of an aliquot of the reaction mixture was added to 30 µL of the Luciferase Assay Reagent (Promega), and luminescence was measured as translational activity using a luminometer (GloMax ...
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bioRxiv - Biochemistry 2024Quote: ... 99 ng of RRE reporter plasmid (pCMV gagpol-IRES FFL-RRE) and 1 ng of the reference plasmid pNL1.1.TK [Nluc/TK] (Promega, N1501) were co-transfected with Lipo-fectamine™ 3000 (Invitrogen™ ...
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bioRxiv - Biochemistry 2024Quote: ... The samples were then digested with 1 μg of Lysyl-endopeptidase (Wako) at room temperature for 2 hours and further digested overnight with 1:50 (w/w) trypsin (Promega) at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... 2 μL of the reaction product was combined with 30 μL of a 1:50 dilution of Nano-Glo substrate (Promega) into a 364-well microplate ...
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bioRxiv - Cell Biology 2024Quote: ... followed by a 4-fold dilution with 50 mM HEPES buffer pH 8.5 and overnight trypsin digestion (50:1 protein : enzyme by weight, Promega V5280) at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were digested at room temperature overnight using 1:100 (m/m) sequencing grade trypsin (cat. no. V5111, Promega, USA).
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bioRxiv - Cancer Biology 2024Quote: ... Thermolysin proteolysis was performed in 100uL of 50mM ABC/10mM KCl (pH=8) by adding Thermolysin (Promega, 1:50 ratio) to proteins and incubating at 70°C for 1 hour at 900 rpm prior to acidification to 1% FA ...
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bioRxiv - Cell Biology 2024Quote: ... A 250 ug was alkylated and digested by incubating overnight at 37oC in trypsin at a ratio of 1:50 (Promega). Digestion was acidified by adding formic acid (FA ...
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bioRxiv - Cell Biology 2024Quote: ... Beads were washed with 100 µL ABC and supernatant was transferred to the digestion and the mixture was digested with 1 µg Trypsin (Promega) overnight at RT ...
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bioRxiv - Cell Biology 2020Quote: ... the artificially synthesized PlGF 3’ untranslated regions (UTR) gene fragment was constructed into pMIR-reporter (Promega, Madison, WI, USA). A complementary sequence with mutation of the seed sequence was designed based on the wild type (WT ...