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Citations for Promega :
3201 - 3250 of 4685 citations for 6 Isoquinolinol 1 2 3 4 tetrahydro 1 4 methylphenyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: The 3’UTR of the genes of interest was cloned in the pSI-Check2 vector (Promega) using the primers listed in Supplementary Table S6 ...
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bioRxiv - Immunology 2024Quote: ... 3×106 ADCC and ADCP Bioassay Effector Cells expressing the human FcγRIIIa or FcγRIIa receptors (Promega) were added per well and the cells were incubated in at 37°C with 5% CO2 for 6 hours ...
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bioRxiv - Neuroscience 2024Quote: ... Caspase activity was measured with the Caspase-Glo® 3/7 (Promega Corp., Fitchburg, WI, USA) assay kit ...
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bioRxiv - Microbiology 2023Quote: ... following tryptic digest at 37 °C for 3 h in the same buffer with trypsin (Promega) in a 1:30 ratio (w/w ...
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bioRxiv - Molecular Biology 2023Quote: ... Tryptic digestion was performed for 3 h with 0.1 μg trypsin at 37°C (Promega, V5113) per sample ...
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bioRxiv - Cancer Biology 2023Quote: ... Caspase-3 and −7 activity was measured following three days of treatment (Caspase-Glo #G8093, Promega), while cell viability was obtained after six days of treatment (CellTiter-Glo 3D #G9681) ...
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bioRxiv - Cell Biology 2023Quote: NRK cells were transfected with IP3R-3 siRNA (see below) using Transfast (Promega Corp.; Madison WI) using manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: RNAs including 5’ and 3’ UTRs were synthesized in vitro using T7 RNA polymerase kit (Promega). cDNA from A ...
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Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2024Quote: Genomic fragment containing the murine Rorc 3’UTR was cloned into psiCHECK2 luciferase reporter plasmid (Promega). This construct was also used to generate the let-7 ʻseed’ deletion mutant derivative using the QuikChange Multi Site Mutagenesis Kit (catalog 200514-5 ...
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bioRxiv - Cell Biology 2020Quote: ... The AIO plasmid and the template plasmid were transfected into HAP1 STG-p62 (for mScarlet-AID-NBR1) or HAP1 WT (for GFP-AID-NBR1) cells using FuGene 6 transfection reagent (Promega, cat# e2691). 48h later the cells were sorted for mScarlet fluorescence in bulk ...
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bioRxiv - Microbiology 2020Quote: ... The env gene clones together with the env-deficient HIV-1 plasmid DNA (SG3Δenv) were contransfected into HEK293T cells (ATCC, Manassas, VA, USA) using the FuGene 6 transfection reagent (Promega, Madison, WI, USA). Two days after transfection ...
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bioRxiv - Biochemistry 2022Quote: ... (complete media) were co-transfected with our library of retinopathy variants and the Bxb1 recombinase expression vector using Fugene 6 (Promega, Madison, WI). Doxycycline (2 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... L981F. Pseudovirions were produced in HEK 293T/17 cells (ATCC cat. no. CRL-11268) by transfection using Fugene 6 (Promega, Catalog #E2692). Pseudovirions for 293T/ACE2 infection were produced by co-transfection with a lentiviral backbone (pCMV ΔR8.2 ...
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bioRxiv - Biochemistry 2020Quote: ... The mutation was confirmed by full-length Spike gene sequencing. Pseudovirions were produced in HEK 293T/17 cells (ATCC cat. no. CRL-11268) by transfection using Fugene 6 (Promega Cat#E2692) and a combination of Spike plasmid ...
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bioRxiv - Cancer Biology 2021Quote: ... Plasmids were packaged with the third-generation lentiviral packaging system (VSV-G, pMDLg, and pRSV-REV) and expressed in HEK293T cells via transient transfection with FuGENE 6 (Promega, Madison, WI). Virus-containing supernatants were harvested after 24 and 48 hours ...
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bioRxiv - Immunology 2021Quote: ... The mutation was confirmed by full-length spike gene sequencing. Pseudovirions were produced in HEK 293T/17 cells (ATCC cat. no. CRL-11268) by transfection using Fugene 6 (Promega, Catalog #E2692). Pseudovirions for 293T/ACE2 infection were produced by co-transfection with a lentiviral backbone (pCMV ΔR8.2 ...
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bioRxiv - Immunology 2021Quote: ... All mutations were confirmed by full-length Spike gene sequencing. Pseudovirions were produced in HEK 293T/17 cells (ATCC cat. no. CRL-11268) by transfection using Fugene 6 (Promega Cat#E2692) and a combination of Spike plasmid ...
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bioRxiv - Cancer Biology 2022Quote: ... 1000 and 5000 µM) or remained untreated for 6 days and viability was analyzed using the CellTiter-Glo Luminescent Cell Viability Assay (Promega, Dübendorf, Switzerland) to determine the adenosine triphosphate (ATP ...
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bioRxiv - Biochemistry 2022Quote: ... cells grown in 10 cm dishes in complete media were co-transfected with our library of cystic fibrosis variants and the Bxb1 recombinase expression vectors using Fugene 6 (Promega, Madison, WI). Doxycycline (2 μg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... of Dusp27 from EDL muscle DNA of C57BL/6 mice was inserted into a pGL4.10 promoter-less luciferase vector (Promega, Madison, WI, USA) digested with XhoI ...
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bioRxiv - Immunology 2024Quote: ... 6 μg of each vector was transfected into tumor cells plated on a 6-well plate using FuGENE6 transfection reagent (Promega, cat. E2691) and OPTI-MEM (GIBCO ...
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bioRxiv - Biochemistry 2023Quote: CFTR function measurements were carried out in recombinant stable cells were made from genetically modified HEK293T cells grown in 10 cm dishes in complete media as was previously described.37,38 Briefly these HEK293T cells contain a Tet-Bxb1-BFP “landing pad” where recombination results in the integration of a plasmid into the gDNA of the cell.37 These cells were co-transfected with plasmid and a Bxb1 recombinase expression vector using Fugene 6 (Promega, Madison, WI). Doxycycline (2µg/mL ...
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bioRxiv - Biochemistry 2024Quote: Total RNA was isolated from the cells of 6-well plates treated as above using the Maxwell RSC simplyRNA kit and the Maxwell RSC Instrument (both Promega, Southampton, UK) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription was performed on 1 μg of RNA with random hexamer primers using MMLV-RT enzyme (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 μg of DNA-free RNA was then used for reverse transcription using M-MLV Reverse Transcriptase (Promega) in the presence of RiboLock ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Sperms were released from antheridiophores by applying ddH2O supplemented with RNasin® Ribonuclease Inhibitor (1 u/μl, Promega), collected in a 1.5 mL tube ...
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bioRxiv - Pathology 2022Quote: ... A total of 1 µg of extracted RNA was transcribed into cDNA using GoScript Reverse Transcription Kit (Promega). Quantitative real-time PCR was performed using FastStart SYBR Green Master mix (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... The sample solutions were diluted to 1 M urea with 50 mM ammonium hydrogen carbonate and trypsin (Promega) was added at an enzyme-to-substrate ratio of 1:50 ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA synthesis was subsequently performed using 1 μg total RNA and oligo-dT primers (C110A, Promega, Madison, WI). Transcript levels were assessed via real-time quantitative polymerase chain reaction (qPCR ...
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bioRxiv - Molecular Biology 2020Quote: Transfection of 1 µg of each individual DNA was performed in six-well plates using FuGENE HD (Promega) for HeLa S3 cells according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: Cells were washed twice with PBS then lysed directly in wells using 1× cell culture lysis reagent (Promega). Cell lysates were clarified by centrifugation ...
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bioRxiv - Microbiology 2020Quote: ... Samples were diluted with three volumes digestion buffer (100 mM Tris/HCl pH 8.5, 1 mM CaCl2) and 0.25 μg Trypsin (Sequencing Grade, Promega) were added and incubated o/n at 37 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... beads were washed two extra times in TBS and resuspended in digestion buffer (2M urea, 50Mm Tris-HCL pH7.5, 1 mM DTT) with 1µg of porcine trypsin MS-grade (Promega) for digestion ...
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bioRxiv - Developmental Biology 2019Quote: ... HRP coupled secondary antibodies (1/2,000, sigma) were used and blots were developed using an ECL kit (Promega).
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bioRxiv - Cancer Biology 2019Quote: ... washed two times in lysis buffer and three times in TBS and resuspended in digestion buffer (2M urea, 50Mm Tris-HCL pH7.5, 1 mM DTT) with 1µg of porcine trypsin MS-grade (Promega) for digestion ...
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Vasohibin1, a new IRES trans-acting factor for sequential induction of angiogenic factors in hypoxiabioRxiv - Cell Biology 2019Quote: Eluted protein samples from BIA experiment were digested in gel with 1 µg of trypsin (sequence grade, Promega) at 37°C OVN ...
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bioRxiv - Cell Biology 2019Quote: ... Tryptic digestion was performed by incubating the beads with 1 µg MS-grade TPCK trypsin (Promega, Madison, WI) dissolved in 200 μl of 50 mM ammonium bicarbonate (pH 8.3 ...
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bioRxiv - Bioengineering 2020Quote: ... Total RNA was reverse transcribed using 0.5–1 µg of total RNA and ImProm-II Reverse Transcriptase (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: The Round 1 eluted pool was PCR amplified for 10 cycles with GoTaq Hot Start Polymerase (Promega, M5001) (1X Colorless GoTaq Flexi Buffer ...
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bioRxiv - Neuroscience 2020Quote: ... the following primary antibodies were used at the indicated concentrations: 1:10’000 mouse anti-β3-Tubulin (Promega, G712A), rabbit anti-p-ERK T202/Y204 (CST ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was diluted 1:5 before quantitative analysis by qPCR was done using GoTaq qPCR Master Mix (Promega) following manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2022Quote: ... The eluent was equally divided into three for digestion with either: 33:1 (w/w) trypsin gold (Promega), 25:1 (w/w ...
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bioRxiv - Molecular Biology 2022Quote: ... The urea concentration was then diluted with 17 μl of 50 mM HEPES pH 8.5 and after adding 1 μl of 0.2 mg/ml trypsin (Promega) in 0.2 mM HCl the digestion continued overnight at 37°C with interval mixing at 2000 RPM for 30 s every 15 minutes ...
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bioRxiv - Microbiology 2021Quote: ... sample-filters were incubated in a wet-cell adding 1:50 w/w trypsin: protein ratio (Promega, V5111) solubilized in 120 µl 50 mM TEAB ...
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bioRxiv - Plant Biology 2021Quote: ... filled with 175 μL 0.5x MS-agar medium 1% sucrose and 35μL (50 μg/mL) of Beetle Luciferin (Promega) dissolved in DMSO ...
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bioRxiv - Molecular Biology 2020Quote: ... 293T cells were transfected in 12 well plates with 1 ug of pcDNA3.4 POLGARF or pcDNA3.4 Timer (as a negative control) with Fugene transfection reagent (Promega) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies were either goat anti-mouse IgG HRP or goat anti-mouse IgG HRP (Promega; 1:10,000) respectively.
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested by incubation with either sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin), chymotrypsin sequencing grade (1/50 ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was four-fold diluted and the protein digested in presence of 0.2 M Tris-HCl buffer pH 8.0 and 1 μg of sequencing grade trypsin (Promega) previously dissolved in 20 mM acetic acid ...
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bioRxiv - Biochemistry 2020Quote: ... and added to 50-100 µL ribosome-coupled anti-Flag M2 agarose beads and 1 µL RNasin (Promega) per reaction ...