Labshake search
Citations for Promega :
3101 - 3150 of 3643 citations for Rat Interleukin 8 Receptor Beta IL8RB CXCR2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: Approximately 5x104 HEK293 cells were seeded on poly-L-lysine coated glass coverslips and transfected with expression constructs for SORL1-WT or SORL1-R953C using Fugene 6 Transfection Reagent kit (Promega). 24h post-transfection ...
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bioRxiv - Biochemistry 2023Quote: ... Authentication of this cell line was performed by the Johns Hopkins Genetics Resources Core Facility by short tandem repeat analysis using the PowerPlex 16 HS kit (Promega) within a year of study ...
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bioRxiv - Systems Biology 2023Quote: The concentration of glutamine and glutamate in the media were measured using the luminescence-based approach Glutamine/Glutamate-Glo TM Assay kit (Promega). The method is based on two steps ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product was extracted from 2% agarose gel and purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) according to the manufacturer’s instructions and quantified using a Quantus Fluorometer (Promega, USA). Normalization was performed the counts of individual OTUs in a sample by dividing the total counts of all OTUs within that sample followed by a multiplication by resulting in relative abundance expressed.
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bioRxiv - Microbiology 2023Quote: ... The inoculum was added to HEK293T cells and incubated at 37 ° C with 5% CO2 and the luciferase signal was with analyzed using luciferase assay kit (Promega).
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bioRxiv - Cell Biology 2023Quote: ... Supernatant was transferred into a fresh tube and used to measure luciferase activity with Dual-Luciferase Reporter Assay kit (Promega) on a Lumat LB9507 luminometer (Berthold Technologies) ...
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bioRxiv - Genomics 2024Quote: ... Transfected cells were cultured under standard conditions for 72 hours before being washed with PBS and lysed and processed using materials provided with the Dual-Luciferase Reporter Assay System Kit (Promega).
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bioRxiv - Developmental Biology 2024Quote: ... Digoxigenin-labelled or Fluorescein-labelled mRNA probes were either prepared as PCR probes with primers listed in Table S4 using the Kit GoTaq green master mix (Promega) or as described (Orgeur et al. ...
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bioRxiv - Developmental Biology 2024Quote: ... For RT-qPCR four micrograms of the extracted RNA from each sample were reverse transcribed with gene specific primers using Goscript reverse transcription kit (Promega). Quantitative PCR was performed using Power SYBR Green (Life Technologies) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 1 uL of the genomic DNA extracted using a Wizard Genomic DNA Purification Kit according to the manufacturer’s instructions (A1120, Promega USA), 1 uL Q5 high-fidelity DNA polymerase ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We isolated and purified genomic DNA from 1 mL of the overnight cultures using the Wizard DNA purification kit (Promega). Short-read sequencing (2 x 150bp paired-end reads ...
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bioRxiv - Microbiology 2024Quote: ... difficile strains carrying sLucopt reporter constructs was mixed with 20 µL of reconstituted Nanoglo substrate (50 fold diluted Nanoglo substrate in kit buffer, Promega) in a white ...
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bioRxiv - Systems Biology 2023Quote: ... Their identity was confirmed through cell line authentication carried out by BMR Genomics (Italy) by amplification of 23 STR loci (PowerPlex Fusion System kit, Promega). Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Bioengineering 2024Quote: ... the 1X Renilla-luciferase substrate mixed with a Stop & Glo buffer (100 µL/well, Promega Dual Glo kit, cat # E1960) was added ...
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bioRxiv - Bioengineering 2024Quote: ... Cell viability and in vitro luciferase expression was evaluated via ONE-Glo + Tox luciferase reporter and cell viability assay kit (Promega) and a multimode microplate reader.
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bioRxiv - Bioengineering 2024Quote: The viability of Exp-HLOs was analyzed by using the CellTiter-Glo® 3D cell viability assay kit (Promega; G9681) following the manufacturer’s recommended protocol ...
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bioRxiv - Biochemistry 2024Quote: Kinase activity was measured in a generic kinase reaction buffer (20 mM Tris pH 7.4, 50 mM KCl, 10 mM MgCl2) using the ADP-Glo assay kit (Promega, V6930) and on a commercial CK2 substrate peptide (EMD Millipore ...
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bioRxiv - Cell Biology 2024Quote: ... caspase-3/7 activities in cells were measured using a luminometric assay kit Caspase-Glo 3/7 (Promega, Madison, WI). The amount of luminescence was measured on the Victor3™ multilabel reader (PerkinElmer Inc.).
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bioRxiv - Cell Biology 2023Quote: ... Necrosis was quantified by measuring release of lactate dehydrogenase in 30 µl samples of the medium and apoptosis by determining cell-associated Caspase3/7 activity using the respective kits from Promega Corp. ...
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bioRxiv - Biochemistry 2024Quote: The total RNAs from each cell line (LO2 and Nrf1-/- LO2 cells) were isolated and purified with an RNA extraction kit (Promega) and subsequently all samples were sequenced at the Beijing Genomics Institute (Shenzhen ...
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bioRxiv - Immunology 2023Quote: ... Luciferase and Renilla signals were measured 48 h after transfection by a Dual-Luciferase Reporter Assay Kit (Promega, No. E1980). Data were analyzed and normalized by the division of firefly luciferase activity with that of Renilla luciferase to eliminate transfection efficiency difference.
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bioRxiv - Immunology 2023Quote: ... viral RNA (vRNA) was isolated from plasma samples using the Maxwell Viral Total Nucleic Acid Purification kit (Promega, Madison WI). Next ...
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bioRxiv - Microbiology 2023Quote: ... Caspase 3/7 enzymatic activity in raw cell lysates was measured using a Caspase Glo 3/7 assay kit (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... Cells were pelleted (2000 g x 5 min) and subjected to genomic DNA (gDNA) extraction using the Wizard Genomic DNA Purification kit (Promega), according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... higginsianum genomic DNA used as PCR templates were isolated by Maxwell RSC Plant DNA Kit with Maxwell RSC 48 instrument (Promega) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: 5,000 indicated cells were seeded in each well of 96-well plates followed by treatment with indicated doses of compounds for 72 hrs before cell proliferation analysis by Cell Titer-Glo Kit (G9241, Promega) according to manufacture instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The mixture was incubated at 25°C for 30 minutes and the production of ADP was measured using the ADP-Glo kinase assay kit (Promega). Vmax was calculated after fitting the kinase activity data to the Michaelis–Menten equation ...
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bioRxiv - Plant Biology 2023Quote: ... Infiltrated leaves were harvested 48 hrs after infiltration and luciferase activity was measured immediately using the Dual-Glo Luciferase Assay kit (Promega). Leaf disks 4 mm in diameter were cut and inserted into white 96 well plates with 75 µl of 1x PBS and 75 µl of luciferase assay reagent ...
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bioRxiv - Plant Biology 2023Quote: ... Five micrograms of total RNA were ligated to the RNA GeneRacer oligo adapter and subjected to reverse transcription utilizing Improm-II Reverse Transcriptase kit (Promega). The cDNA was used for amplification of cleaved PYL6 fragments ...
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bioRxiv - Genetics 2023Quote: ... After a second purification using a Zymo DNA Clean and Concentrator kit the PCR product was used as a template in a T7 RiboMAX express RNAi System (Promega) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Cells were immediately stimulated with cytokine for 24 hr and nanoluciferase activity was measured relative to control activity using the NanoGlo Dual Luciferase kit (Promega). The primers for cloning enhancer fragments into pNL3.1 are in Table S3.
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bioRxiv - Immunology 2023Quote: ... total RNA was purified from 3-4 tissues of female flies using a ReliaPrep RNA Tissue Miniprep kit (Promega, z6112). Quantitative RT-PCR was performed using a OneTaq RT‒PCR kit (Promega ...
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bioRxiv - Molecular Biology 2022Quote: ... the luminescence of 50 µl of cell lysate was measured using the Nano-Glo Luciferase Assay kit (Promega, Madison, WI) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... All VLP volumes were calculated and normalized to 1.4 x 107 RLU using the Nano-Glo® HiBiT Lytic Detection System kit (Promega).
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bioRxiv - Microbiology 2022Quote: ... 48 h post transfection cells were harvested for western blotting or luminescence detection using a Firefly Luciferase Assay kit (Promega) and a Glomax luminometer (Promega) ...
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bioRxiv - Microbiology 2023Quote: ... The bacterial cells were collected from 2mL of the culture and the genomic DNA was extracted using the Wizard Genomic DNA Purification Kit (Promega). Samples were then sent to Novogene Co. ...
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bioRxiv - Cell Biology 2023Quote: ... Other parameters of ER Stress induced cell death were measured through immunoblotting or with the Caspase 3/7 Glo Assay Kit (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Phenol-chloroform phase separation and chloroform phase separation steps were repeated to remove the RNase and then DNA was purified from the aqueous phase by using Promega kit protocol (Promega Wizard SV Gel and PCR Clean-Up System ...
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bioRxiv - Zoology 2023Quote: Total RNA was extracted using the Maxwell 16 LEV Plant RNA Kit with the Maxwell 16 Research Instrument (Promega, USA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Genomic extraction for the WT C6706:str2 strain and each of the resistant mutants was performed using Wizard Genomic DNA Purification Kit (Promega). The concentration of DNA was quantified using Nanodrop spectrophotometer (ND-1000) ...
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bioRxiv - Molecular Biology 2023Quote: ... The ratios of luciferase activity for the renilla and firefly luciferases were measured 48 hrs (hours) after transfection using the dual- Luciferase reporter assay kit from Promega and a luminometer ...
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bioRxiv - Microbiology 2023Quote: RT-qPCRs were performed with MLV SU or 2-LTR primers (Table 1) using a Power SYBR green PCR kit (Promega) and the QuantStudio 5 real-time PCR system (Applied Biosystems) ...
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bioRxiv - Microbiology 2023Quote: ... and genomic DNA was isolated from overnight cultures (5 mL) using the Wizard Genomic DNA Purification Kit (Promega GmbH, Walldorf) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was isolated with the Maxwell RSC viral total nucleic acid purification kit on a Maxwell RSC 48 instrument (Promega). Isolated ZIKV RNA was titrated by qRT-PCR using TaqMan Fast virus 1-step master mix (ThermoFisher ...
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bioRxiv - Biochemistry 2023Quote: ... HiBit and firefly luciferase activity were measured simultaneously using a Nano-Glo HiBiT Dual-Luciferase Reporter System kit (Promega, N1630) with purified LgBiT Protein (Promega) ...
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bioRxiv - Biochemistry 2023Quote: ... PCR reaction mixtures were solved in 1% (w/v) agarose gel and purified using Wizard SV gel & PCR Clean-Up kits (Promega). Then ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with or without 50 μM adenosine 5′-triphosphate (ATP) at 30 °C for 30 minutes followed by kinase assays using ADP-Glo Kinase Assay kit (Promega). Data were analyzed using GraphPad Prism 9 ...
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bioRxiv - Cancer Biology 2022Quote: ... for 72 hours and cell proliferation was assayed using the CellTiter 96 Non-Radioactive Cell Proliferation kit and a GloMax Discovery plate reader (Promega). For proteomic mass spectrometry ...
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bioRxiv - Cancer Biology 2023Quote: ... All cell lines were validated by DNA fingerprinting through the University of Colorado Molecular Biology Service Center utilizing the STR DNA Profiling PowerPlex-16 HS Kit (DC2101, Promega)(Extended Data Figure 11).
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bioRxiv - Genomics 2023Quote: DNA was extracted from the agar/cell mixture from the output and control pools using a Wizard Genomic DNA Purification Kit (Promega). The manufacturer’s protocol was followed with two modifications ...