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Citations for Promega :
3001 - 3050 of 3827 citations for Human Bcl2 Associated Death Promoter BAD ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... The anti-proliferative effects of these compounds were assessed using the Cell Titer Glo assay kit (Promega Cat. # G7570) following manufacturer protocol ...
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bioRxiv - Biophysics 2022Quote: ... Cells were stimulated on the next day and lysed after 24 hrs following manufacturer’s protocol in Dual Luciferase Assay kit (Promega). Luminance signal readings were performed using or SpectraMax i3x (Molecular Devices) ...
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bioRxiv - Microbiology 2022Quote: ... [32P]-dCTP labelled hmbA and 18S rRNA gene-specific DNA molecules were used as gene probes using the random hexanucleotide-primer kit following the supplier’s instructions (Promega). As a loading control ...
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bioRxiv - Microbiology 2022Quote: ... PPMP was assessed for cytotoxicity at the indicated concentrations with a CytoTox96 non-radioactive cytotoxicity colorimetric assay kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Total RNA was extracted from 293T cells using an Eastep Super Total RNA Extraction Kit (LS1040, Promega, WI, USA). Reverse transcription was conducted using GoScriptTM Reverse Transcription Mix and Random Primers (A2800 ...
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bioRxiv - Immunology 2022Quote: ... the samples were thawed and aliquoted in technical duplicates for detection of firefly and renilla luciferase activity using the Dual-Luciferase Reporter Assay kit using the GloMax luminometer to the manufacturer’s specifications (Promega). Promoter activity was determined by normalising firefly luciferase values to renilla luciferase values and expressed as relative light units (RLU).
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bioRxiv - Microbiology 2022Quote: PCR amplicons were gel extracted using the QIAquick gel extraction kit and ligated into pGEM-T Easy plasmid (Promega). Vectors containing the PCR products were then cloned into chemically competent E ...
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bioRxiv - Neuroscience 2023Quote: ... the luminescence detection assay was performed following all the manufacturer’s recommendations (Dual-Luciferase Reporter Assay Kit System E1910 (Promega).
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bioRxiv - Biochemistry 2023Quote: ... The number of viable cells was determined using CellTiter 96 AQueous One Solution Cell Proliferation Assay kit (Promega G3580) and absorbance at 490 nm was measured by a plate reader (CLARIOstar ...
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bioRxiv - Genomics 2023Quote: ... Protocol for cell lysis and measurement of the activities of the firefly luciferase (Dual-Luciferase Reporter Assay kit, Promega), with normalisation using the Renilla luciferase activity ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were collected for a luciferase activity assay using a dual-luciferase assay kit (Promega, Madison, WI, USA). The normalized luciferase activity was obtained by using the formula ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Total RNA was extracted using the Maxwell® RSC instrument together with the Maxwell® simplyRNA Tissue Kit (Promega) according to the manufacturer’s instructions with a final elution volume of 60 μl ...
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bioRxiv - Cell Biology 2023Quote: ... lactate dehydrogenase (LDH) release assay was performed using CytoTox 96 Non-Radioactive Cytotoxicity Assay kit (Promega, Madison, Wisconsin, USA) according to manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2023Quote: ... All mRNAs were prepared using the Ambion mMessage Machine kit using Sp6 (#AM1340) supplemented with RNAse Inhibitor (Promega #N251B).
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bioRxiv - Developmental Biology 2023Quote: ... Ghost Database KY21.Chr1.1715) we extracted genomic DNA from local Norwegian animals using the Wizard Genomic DNA Purification Kit (A1120, Promega). We then used the purified gDNA at a concentration of 100-150ng/μl ...
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bioRxiv - Immunology 2023Quote: ... Cells were lysed on the plate and incubated with luminescence glo regents per the luminescence glo assay kit’s instructions (Promega). Luminescence was recorded using a BioTek Synergy2 plate reader.
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bioRxiv - Cell Biology 2023Quote: DNA was prepared from bacterial cultures grown at 37°C using a Midiprep kit (PureYield Plasmid Midiprep System, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: The intracellular NAD+ in 3T3-L1 cells or ASCs was measured using the NAD+/NADH-Glo assay kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 400 ng of total RNA was transcribed to cDNA using the ImProm-II™ reverse transcription kit (Promega, A3500) with random primers ...
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bioRxiv - Molecular Biology 2023Quote: ... and Renilla and Firefly luciferase activities were measured on a luminometer using the Promega dual luciferase kit (Promega, E1910) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was determined using the CellTiter 96 Aqueous One Solution Cell Proliferation Assay (MTS) kit (Cat. #G3580, Promega) at 24 ...
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bioRxiv - Genetics 2023Quote: ... RNA (1000 ng input) was subsequently reverse-transcribed to cDNA using random primers and the GoScript RT kit (Promega). RT-qPCR was performed using FIREPoly qPCR Master Mix (Solis BioDyne ...
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bioRxiv - Genomics 2023Quote: ... Protein was precipitated by the addition of 200 μl Protein Precipitation Solution (Promega Wizard HMW DNA Extraction Kit A2920). Using a wide bore tip the samples were mixed by drawing up contents from the bottom of the tube and then expelled on the side of the tube 5 times ...
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bioRxiv - Genomics 2023Quote: ... The total cellular DNAs of the activated CD4+ T cells were isolated using a DNA extraction kit (Promega Wizard) for analysis.
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bioRxiv - Microbiology 2023Quote: ... Total genomic DNA was isolated from overnight cultures of Ag1 and Ag2 using the Genome Wizard kit (Promega, WI), following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... cells were collected to measure the firefly luciferase and renilla luciferase luminescent activities using the Dual-Luciferase Reporter Assay kit (Catalog:E1910, Promega) with 96-well plates (Nunc MaxiSorp ...
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bioRxiv - Microbiology 2023Quote: ... The sequence of the plasmid was verified by sequencing using a SupreDye v3.1 Cycle Sequencing Kit (M&S TechnoSystems, Osaka, Japan, Cat# 063001) with a Spectrum Compact CE System (Promega). To generate a pDON-5 Neo-vector expressing IFNAR1 with the W70C mutation ...
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bioRxiv - Synthetic Biology 2024Quote: ... Females from each progeny were then pooled to perform genomic DNA extraction (Wizard® Genomic DNA Purification Kit, Promega) and subsequently do amplicon sequencing on the dsx target to check the frequency of the dsxFΔ11 allele in the offspring ...
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bioRxiv - Microbiology 2024Quote: The oxidized glutathione (GSSG) and reduced glutathione (GSH) were individually measured by employing the Glutathione Assay Kit (Promega, USA) as described by Nakagawa et al ...
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bioRxiv - Cell Biology 2024Quote: ... we isolated individual clones and screened for expression using the C-terminal nanoluc fusion (Nanoluc Lytic Detection Kit, Promega), followed by additional characterization of SMO agonist-induced ciliary accumulation via microscopy ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The plasmid DNA was purified from 4-mL overnight cultures by use of the Wizard Plus MiniPrep kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... The ground material was used for genomic DNA extraction with the RSC Plant DNA Kit (Promega, Madison, WI, USA) using the Maxwell® RSC device according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Samples were prepared according to the directions from the Wizard HMW DNA Extraction kit for Gram-negative Bacteria (Promega).56 The genomic DNA was rehydrated in DNA Rehydration Solution and Illumina sequenced (SeqCoast) ...
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bioRxiv - Molecular Biology 2024Quote: ... The linearized plasmid was then purified using the Wizard® SV gel and PCR clean-up system kit (Promega) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... The sequences of all the plasmids were verified using a SupreDye v3.1 Cycle Sequencing Kit (M&S TechnoSystems, Osaka, Japan, Cat# 063001) with a Spectrum Compact CE System (Promega).
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bioRxiv - Microbiology 2024Quote: Bacterial DNA of both wildtype and mutants were purified with a Wizard genomic DNA purification kit (Promega, Wisconsin, US). DNA was then tagged and multiplexed with the Nextera XT DNA kit (Illumina ...
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bioRxiv - Cell Biology 2024Quote: ... and equal amounts of RNA per sample were transcribed into cDNA using the GoScriptTM Reverse Transcription Kit (Promega #A5000). Quantitative PCR (qPCR ...
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bioRxiv - Cancer Biology 2024Quote: ... tumoral area was macrodissected prior DNA extraction using Maxwell 16 FFPE LEV DNA Purification Kit (Promega, Madison, WI, USA). DNA from the 60 non-CCHD carotid bodies were previously extracted and passed internal quality control at the VHIO’s laboratory before sequencing ...
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bioRxiv - Microbiology 2024Quote: ... Twenty-four hours after transfection cells were harvested for luciferase assay using the Dual-Glo Reporter Assay Kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: cDNA levels were measured by quantitative PCR using the GoTaq® qPCR kit (Promega Ref A6002, Madison, WI, USA) according to the manufacturer’s instructions under standard cycling conditions ...
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bioRxiv - Cell Biology 2024Quote: ATP levels in conditioned media and serum were measured by RealTime-Glo Extracellular ATP Assay kit (Promega, Madison, WI).
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bioRxiv - Physiology 2024Quote: Chymotrypsin-like 20S Proteasome activity was measured via a commercially available kit (Promega, Madison, WI, USA; Cat No. G8622) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: The concentration of NO in the cell culture medium was measured using a Griess Assay Kit (Promega, Mannheim, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... followed by gel purification of the products with a Wizard SV Gel and PCR Clean-Up System kit (Promega). Transcription products were separated on a 6% denaturing polyacrylamide gel (AccuGel 19:1 ...
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bioRxiv - Biochemistry 2024Quote: ... I1 Intracellular luciferase activity (Relative light units, RLU) was measured using the Bright-Glo Luciferase Assay Kit (Promega, E2620) and detected with a GloMax 20/20 Luminometer (Promega ...
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bioRxiv - Immunology 2024Quote: ... DY401-05) and/or relative LDH levels (as a surrogate for pyroptosis) using a CytoTox 96 Kit (Promega; G1780).
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bioRxiv - Biochemistry 2024Quote: ... Colonies were pooled with LB and samples were diluted to an OD600 of ∼4.0 and 1 mL of concentrated cells underwent genomic DNA preparation using the Wizard Genomic DNA Purification Kit (Promega). DNA was quantified using the QuantiFluor ONE dsDNA System (Promega) ...
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bioRxiv - Biochemistry 2024Quote: ... Each library was diluted to an OD600 of ∼4.0 and 1 mL of concentrated cells underwent genomic DNA preparation using the Wizard Genomic DNA Purification Kit (Promega). DNA was quantified using the QuantiFluor ONE dsDNA System (Promega) ...
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bioRxiv - Systems Biology 2024Quote: ... the amount of total ATP produced was measured using a luminescence assay kit according to the manufacturer’s instructions (Promega). Fold change was calculated for the relative luminescence against non-antibiotic treated control.
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bioRxiv - Biophysics 2024Quote: ... Complementary DNAs were obtained with the SuperScript II Reverse Transcriptase kit (18064022, Thermofischer Scientific) and random hexamers (C1181, Promega) according to the manufacturer’s instructions ...