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Citations for Promega :
2951 - 3000 of 3879 citations for 6 Bromo 2 trifluoromethylimidazo 1 2 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... viral RNA was quantified by real-time RT-qPCR (GoTaq 1 step RT-qPCR kit, Promega). Quantification was provided by serial dilutions of an appropriate RNA standard ...
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bioRxiv - Zoology 2024Quote: One-step RT-qPCR was conducted using GoTaq 1-Step RT-qPCR System kit (Promega, France) in total reaction volume of 20 µl containing 2 µl of RNA extracts and 300 nM of the same forward and reverse primers as above ...
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bioRxiv - Neuroscience 2024Quote: ... 0.1-1 µg of RNA was reverse transcribed into cDNA using M-MLV reverse transcriptase (Promega) and oligo(dT ...
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bioRxiv - Molecular Biology 2023Quote: ... One-step RT-qPCR was performed using the GoTaq 1-Step RT-qPCR System (Promega, #A6020) and MicroAmp Fast Optical 96 Well Reaction Plates (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... After the translation reaction was quenched by adding 30 μl of 1× Passive Lysis Buffer (Promega), 10 μl of the mixture was placed on a 96-well white assay plate (Coster) ...
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bioRxiv - Microbiology 2023Quote: RT-qPCR was performed using the GoTaq 1-Step RT-qPCR kit (Promega Cat. No. A6020) using nifH and 16S rRNA (reference gene ...
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bioRxiv - Molecular Biology 2023Quote: ... to the 1x PBS. PD-1/PD-L1 blockade bioassay (cat. J1250) was purchased from Promega.
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bioRxiv - Neuroscience 2023Quote: ... At DIV12 cells are lysed in Passive lysis buffer (diluted 1:5 in MilliQ-water, Promega) for 15min and dual luciferase assay performed using home-made reagents according to Baker and Boyce (63 ...
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bioRxiv - Biochemistry 2023Quote: ... 10 µg of reconstituted protein was digested in 0.05 N HCl with 1 µg pepsin (Promega) without prior reduction/alkylation at a volume of 200 µl 50 °C for 3 h ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were resuspended in 1 mL of the same buffer containing protease inhibitor cocktail (Promega, USA), and then transferred to 2 mL lysing matrix B tubes containing 0.1 mm diameter silica beads (MP Biomedicals ...
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bioRxiv - Bioengineering 2024Quote: ... RT-qPCR was performed using the GoTaq Probe 1-Step RT-qPCR System (Promega Cat# A6120). RT-qPCR primers and probes were listed in Supplementary Data 2.
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bioRxiv - Microbiology 2023Quote: ... Complimentary DNA was prepared from 1 µg of RNA using a Reverse Transcription Systems Kit (Promega). Quantitative PCR (qPCR ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was synthesized from 1 µg of total RNA using M-MLV reverse transcriptase (Promega, #M1701) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... we applied 100 μl of 1:5 diluted cell culture lysis reagent (Promega, Cat. No.: E1531) to each well ...
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bioRxiv - Molecular Biology 2024Quote: ... The solution was diluted four times and digested with Trypsin at 1:50 (w/w; Promega).
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bioRxiv - Cancer Biology 2024Quote: ... qPCR reactions were performed using the GoTaq 1-Step RT-qPCR System (Promega GmbH, Walldorf, Germany). 100 ng of isolated mRNA were used per reaction ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were digested with Lys-C (Wako Chemicals) and trypsin (1:40 ratio, Trypsin Gold, Promega). After complete digestion had been confirmed by nanoLC-MS/MS (<7% missed cleavage sites) ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were diluted to 1 M urea with 50mM ammonium bicarbonate and digested with chymotrypsin (Promega) at 25°C overnight ...
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bioRxiv - Immunology 2024Quote: ... and samples were digested overnight with a 1:100 (w/w) Trypsin/Lys-C Mix (Promega) at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... The resuspended proteins were digested in trypsin/Lys-C mix (1:25 protease: protein ratio; Promega) for 16 hours at 37 °C in a thermomixer ...
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bioRxiv - Immunology 2024Quote: ... caspase-1 biosensor mice were weighed and injected intraperitoneally with 150 mg/kg VivoGlo Luciferin (Promega). Mice were anesthetized with 2% isoflurane/air mixture and imaged 10 minutes following administration of the luciferase substrate using the IVIS 100 Imaging system (Xenogen) ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were diluted to 1 M urea with 50 mM ambic and digested with Trypsin (Promega #V5111) for 10 hours at 1:125 enzyme:protein at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... ribosome positioning was determined by primer extension/reverse transcription using AMV reverse transcriptase (1 U/μl) (Promega) in the presence of 125 μM of each of dTTP ...
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bioRxiv - Microbiology 2019Quote: ... The pellet was dissolved in 1% (w/v) RapiGest (Waters) (10 µL) and trypsin (Sequencing grade, Promega) solution was added (90 µL ...
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bioRxiv - Molecular Biology 2021Quote: ... lysed in 1 × passive lysis buffer and analyzed using the Dual-Luciferase Reporter Assay System (Promega Corporation) and a SpectraMax L Microplate Reader (Molecular Devices ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated for 1 h with the secondary antibody anti-mouse IgG HRP conjugate (W4021, Promega, U.S.A.) diluted at 1/3000 in 10% Blocking One/TTBS ...
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bioRxiv - Microbiology 2020Quote: ... the blots were incubated for 1 hour with secondary antibody (goat anti-rabbit IgG-HRP, Promega W4018) that was diluted 1:10000 in blocking buffer ...
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bioRxiv - Microbiology 2019Quote: ... The reaction mixture (25 μL) used for cDNA synthesis contained 1⋅25 μM random hexamer primers (Promega), 250 μM of each deoxynucleoside triphosphate (dNTP) ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein digestion on beads was carried out using a ratio 1:50 sequencing grade modified trypsin (Promega): protein extract in 100 mM ammonium bicarbonate at 37 °C overnight ...
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bioRxiv - Immunology 2019Quote: ... In vitro pmel-1 T cell-mediated cytotoxicity assay with CytoTox-ONE Homogeneous Membrane Integrity Assay (Promega). protocols.io dx.doi.org/10.17504/protocols.io.6j4hcqw
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bioRxiv - Developmental Biology 2021Quote: ... the reaction mixtures for in vitro transcription were treated with RQ-1 RNase-free DNase I (Promega). Synthesized mRNAs were treated with phenol-chloroform to remove protein components ...
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bioRxiv - Microbiology 2020Quote: ... cells were transfected with 500 ng of either HDM-SARS-Spike-delta21 or HDM_Spike_RBD_B7-1 plasmids and 1.5 ng of Transfection Carrier DNA (Promega, E4881) using BioT reagent (Bioland Sci ...
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bioRxiv - Microbiology 2020Quote: ... RNA (1 μg) was converted to cDNA using the GoScript™ Reverse Transcription System (Promega, Madison, WI). The cDNA was synthesised was used for qPCR using the iQTM SYBR® Green Supermix (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2021Quote: ... Dilutions of antibodies applied were: rabbit anti-ferritin 1:10000 (primary) and anti-rabbit HRP conjugated (Promega) 1:10000 (secondary).
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bioRxiv - Microbiology 2021Quote: ... At 1 × s after injection of 20 µl of the substrate solution (Renilla Luciferase Assay System, Promega), relative light units (RLUs ...
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bioRxiv - Neuroscience 2020Quote: ... Tissues were then stained using the following primary antibodies overnight: mouse anti-β Galactosidase (1:1000; Promega), rabbit anti-Er81 (1:5000 ...
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bioRxiv - Cell Biology 2020Quote: 1 µg of total RNAs were reverse transcribed with Moloney murine leukemia virus Reverse Transcriptase (M170B, Promega). 100 ng cDNA and FastStart Universal SYBR Green Master (04913850001 ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were digested in-gel using trypsin (Gold Promega, 1 μg per band, overnight at 30°C,) as previously described (Thouvenot et al. ...
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bioRxiv - Microbiology 2022Quote: ... Larvae were immobilized in the dish using a drop of 1% low-melting-point agarose (V2111, Promega) solution in 80% glycerol ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were then incubated with 1:50 enzyme to protein ratio of sequencing-grade trypsin (Promega) overnight at 37 °C ...
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bioRxiv - Biophysics 2022Quote: ... The embryos were then incubated in a 1:5,000 dilution of TMR HaloTag Ligand (5 mM; Promega) in Danieau’s medium ...
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bioRxiv - Plant Biology 2022Quote: ... Protein expression was confirmed by Western blot with Anti-Halo Tag monoclonal antibody (1:2000) (G9211, Promega).
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bioRxiv - Biochemistry 2022Quote: ... the samples were incubated with trypsin (trypsin to substrate ratio of 1:50, Promega, cat. no. V5111) and 1 mM CaCl2 for 16 h at 37°C ...
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bioRxiv - Plant Biology 2021Quote: ... For multiple day measurements plants were sprayed daily with 1 mM D-luciferin (Promega, Fitchburg, Wl, USA) at 10 am ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were incubated with cell-permeant TMR red Halo-tag ligand (1:2,000 for 15 min, Promega) 24 hours post-transfection ...
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bioRxiv - Microbiology 2020Quote: ... and 1 μg of a plasmid expressing the VSV G glycoprotein using the FuGene HD reagent (Promega). Forty-eight hours later ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were then incubated with 1:50 enzyme to protein ratio of sequencing-grade trypsin (Promega) overnight at 37 °C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Bacterial DNA was extracted from 1 ml of culture using the Wizard genomic DNA purification kit (Promega). Evolved population DNAs were sequenced at the GeT-PlaGe core facility (https://get.genotoul.fr/) ...
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bioRxiv - Developmental Biology 2019Quote: ... Immuno-signals were visualized with an alkaline-phosphatase (AP)-conjugated anti-rabbit IgG antibody (1:5000, Promega). Western blot revelation was performed using a cheminoluminescence digital imaging system (ImageQuant Las-4000 Mini ...
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bioRxiv - Immunology 2019Quote: ... at 37ºC for 1 hr followed by treatment with 20 µg proteinase K (Promega, Madison, WI, USA) at 45ºC for 1 hr to remove protein ...