Labshake search
Citations for Promega :
251 - 300 of 410 citations for Recombinant Human Metadherin GST tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... 60,000 nuclei were sorted using an SH800 sorter (Sony) into 50 μl of collection buffer [1.0U/μL Recombinant RNAsin (Promega, PAN2515), 5% Fatty-acid-free BSA in PBS (Proliant ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Initial P0 virus was obtained by addition of ∼3 µg recombinant bacmid DNA mixed with 3 µl FuGENE HD Transfection reagent (Promega) in 100 µl Sf900 II media (Invitrogen ...
-
bioRxiv - Biochemistry 2023Quote: ... then alkylated (100 mM iodoacetamide in 100 mM ammonium bicarbonate at room temperature for 45 minutes) prior to in-gel trypsin digestion (30 μL of 6 ng/μL recombinant sequencing-grade trypsin (Promega) in 100 mM ammonium bicarbonate per cubed band ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... 100 ng of either XeARPP19 or ClyARPP19 were incubated with 200 μM ATP and 25 units of recombinant bovine PKA (Promega) in PKA Buffer for 3 hours at 37°C under 1200 RPM stirring ...
-
bioRxiv - Cancer Biology 2023Quote: ... recombinant methylated BCAR3 (see methylation assay method) was sliced from the gel and digested with modified trypsin (sequencing grade, Promega). The resulting peptides were analyzed by online nano liquid chromatography (LC)-MS/MS (UltiMate 3000 RSLCnano and Q-Exactive HF ...
-
bioRxiv - Microbiology 2024Quote: ... Recombinant pNZ8037 plasmid carrying wild-type or mutated ptnCD genes was isolated using PureYieldTM Plasmid Mini-prep System (Promega, USA). Targeted changes in the ptnCD genes were introduced following the Site Directed Mutagenesis Cornell iGEM 2012 Protocol (http://2012.igem.org/wiki/images/a/a5/Site_Directed_Mutagenesis.pdf) ...
-
bioRxiv - Developmental Biology 2024Quote: Substrate titration kinase reactions were performed on the indicated recombinant LIN-42 proteins (CK1BD + Tail WT or mutants) using the ADP-Glo kinase assay kit (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... 2 µg of anti-DNA-PKcs antibody or the corresponding IgG control was incubated with 70 ng of recombinant DNA-PKcs protein (Promega) in 500 µL of lysis buffer (20 mM Tris–HCl [pH 7.4] ...
-
bioRxiv - Molecular Biology 2020Quote: ... and pooled human male DNA (G3041, Promega and 4312660, Life Technologies) were used to generate calibration curves and serve as reaction controls ...
-
bioRxiv - Biochemistry 2020Quote: SETD2-HaloTag® human ORF in pFN21A was procured from Promega. Deletion mutants of SETD2 were constructed by PCR (Phusion polymerase ...
-
bioRxiv - Biochemistry 2020Quote: SETD2-HaloTag® human ORF in pFN21A was procured from Promega. Deletion mutants of SETD2 were constructed by PCR (Phusion polymerase ...
-
bioRxiv - Cancer Biology 2022Quote: ... Human 26S proteasome purified from HEK293 cells was purchased from Promega. The proteasome chaperoning reaction tests were carried out as follows ...
-
bioRxiv - Cell Biology 2021Quote: ... Human normal genomic DNA was obtained from Promega (Madison, WI, USA) and the values are averages of three replicates ...
-
bioRxiv - Cancer Biology 2020Quote: ... in a background of 130ng of normal human genomic DNA (Promega) was used as a positive control ...
-
bioRxiv - Cancer Biology 2024Quote: ... 830bp or 800bp human STIM1 promoter into pGL3 basic vector (Promega) between XhoI and HindIII ...
-
bioRxiv - Cancer Biology 2024Quote: ... 800bp or 300bp human HNF4α promoter into pGL3 basic vector (Promega) between XhoI and HindIII ...
-
bioRxiv - Neuroscience 2020Quote: ... after which beads were washed with 1X High Salt Wash Buffer(50mM Tris-HCl pH 7.4, 350mM NaCl, 1%NP-40 and 1 unit/ul Promega recombinant RNAsin). In the last wash ...
-
bioRxiv - Biochemistry 2021Quote: ... RRL were incubated for 5 min at 30°C then 10 min on ice with buffer containing 1U/μl of recombinant RNasin (Promega®), 75 mM KCl 0.5 mM MgCl2 ...
-
bioRxiv - Developmental Biology 2024Quote: ... The katna1 digoxigenin-labelled sense and antisense riboprobes were synthetized from the linearised recombinant TOPO® TA cloning vector using T7 and T3 RNA Polymerase (Promega) according to the supplier’s instructions ...
-
bioRxiv - Biophysics 2023Quote: ... The initial recombinant baculovirus was generated by transfecting adherent Sf9 cells with the bacmid DNA using the FuGENE HD reagent (Promega, USA) and used to infect suspension cells for virus amplification ...
-
bioRxiv - Systems Biology 2020Quote: ... albicans as well as of a commercially available human protein digest (Promega), which was exclusively used in this experiment ...
-
bioRxiv - Cell Biology 2023Quote: ... human BLTP2/KIAA0100 ORF was amplified from pFN21A-Halo-KIAA0100 (FHC00016, Promega). An unstructured linker (sequence ...
-
bioRxiv - Biochemistry 2023Quote: MS-Compatible Human Protein Extract Digest (K562) was purchased from Promega (V6951), MassPREP E ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... The recombinant plasmid was digested with Sac1 and Nde1 restriction enzyme and sub-cloned in pGL3 Basic luciferase vector (Promega, Madison, USA).
-
bioRxiv - Biochemistry 2020Quote: ... Moloney murine leukemia virus reverse transcriptase (MMLV-RT) enzyme and recombinant RNasin® ribonuclease inhibitor were purchased from Promega (Madison, WI, USA). Evagreen 2X qPCR MasterMix was purchased from Applied Biological Materials Inc ...
-
Berberine is a novel mitochondrial calcium uniporter (MCU) inhibitor that disrupts MCU-EMRE assemblybioRxiv - Cell Biology 2024Quote: ... biotin or biotin-labeled berberine (2 μg) was pre-incubated with either recombinant protein (2 μg) or in vitro transcribed/translated protein (Promega, Cat# L1170) for 8 hours at 4°C ...
-
bioRxiv - Genomics 2022Quote: ... The primers were commercially synthesized (IDT) and tested on human genomic DNA (Promega) to confirm generation of only one amplicon product at the expected size ...
-
bioRxiv - Genetics 2020Quote: ... Deidentified healthy donor DNA was obtained from Promega (Human Genomic DNA: Female, G152A). DNA from ALS cases (ND11836 and ND13803 ...
-
bioRxiv - Cancer Biology 2020Quote: 2000bp human lncRNA-TANAR(ENST00000425110.1) promoter was cloned into PGL3 basic vectors (Promega). By mutating the crucial site of AR binding site in the lncRNA-TANAR 5’ promoter to EcoRI cutting site (-GAATTC) ...
-
bioRxiv - Genomics 2022Quote: ... diluted to 1% in purified human genomic DNA (Promega, female, catalog No. G1521). Briefly ...
-
bioRxiv - Biochemistry 2023Quote: A whole-cell protein extract from human K562-cells (Promega, V6941, lot 444583) was dissolved in 50 mmol/L Tris and 6.5 mol/L urea ...
-
LRBA balances antigen presentation and T-cell responses via autophagy by binding to PIK3R4 and FYCO1bioRxiv - Immunology 2024Quote: Human LRBA was cloned in seven different fragments into pCIneo FLAG vectors (Promega). These plasmids were kindly provided by Dr ...
-
bioRxiv - Biochemistry 2021Quote: ... Pull down assays were performed utilizing myc-tagged proteins that were generated from recombinant pGBKT7-derivatives through in vitro transcription/translation using wheat germ extract (cat# L4330, Promega, Madison, WI, USA)(Stephan et al. ...
-
bioRxiv - Microbiology 2023Quote: ... As a qPCR standard the recombinant plasmid pBELX-1 (Bellanger et al., 2014) was extracted using the Wizard plus SV Miniprep DNA Purification System (Promega, Madison, WI, USA) according to the manufacturers’ instructions and linearized by restriction enzyme BamHI (Promega ...
-
The absence of C-5 DNA methylation in Leishmania donovani allows DNA enrichment from complex samplesbioRxiv - Molecular Biology 2020Quote: ... we used a 1/1500 artificial mix of promastigote DNA and human DNA (Promega) to reflect the median ratio found in clinical samples ...
-
bioRxiv - Neuroscience 2022Quote: Open reading frames of human OR genes were subcloned into pCI (Promega, WI, USA) with a Rho-tag (the sequence encoding the first 20 amino acids of rhodopsin ...
-
bioRxiv - Biochemistry 2024Quote: ... 24 hours after transient transfection with mouse or human ENPP3 via Fugene 6 (Promega), the media was gently removed and replaced with serum-free DMEM supplemented with 1% insulin-transferrin-selenium-sodium pyruvate (ThermoFisher ...
-
bioRxiv - Immunology 2023Quote: ... tailed with complete TRAC and TRBC1 gene segments amplified from human genomic DNA (Promega), respectively ...
-
bioRxiv - Systems Biology 2024Quote: ... Cells were transfected with the human FOXM1 promoter (19) and Renilla (pRL-TK, Promega) as an internal transfection control using Lipofectamine LTX (#15338100 ...
-
bioRxiv - Genomics 2024Quote: ... The human promoters HSPA1A and DHDH were cloned into the pGL4.12 vector (Promega, #E6671) at the BglII and HindIII restriction sites ...
-
bioRxiv - Plant Biology 2020Quote: ... The recombinant Halo-His-gene was expressed using a 30 μL TNT®SP6 High-Yield Wheat Germ Protein Expression System (Promega, USA, Catalog No L3261). Expression levels were determined by SDS-PAGE and western blot ...
-
bioRxiv - Cancer Biology 2021Quote: ... lentiviral packaging vectors into human HEK-293T cells via calciumphosphate transfection (Promega; Madison, WI, USA), according to manufacturer’s directions ...
-
bioRxiv - Cancer Biology 2021Quote: The 3’UTR of human PAQR3 was cloned into pmirGLO vector (Promega, Madison, WI, USA), and then validated by sequencing ...
-
bioRxiv - Molecular Biology 2021Quote: ... Human SMARCB1 was translated in vitro using TNT Quick Coupled Transcription/Translation System (L1170, Promega). 1 µg of pcDNA3.1-FLAG-SMARCB1 was incubated at 30°C for 90 minutes with 20 µM methionine and TNT T7 Quick Master Mix ...
-
bioRxiv - Cancer Biology 2020Quote: ... Human cell lines were DNA fingerprinted for provenance using the Power-Plex 1.2 kit (Promega) and confirmed to be the same as the DNA fingerprint library maintained by ATCC ...
-
bioRxiv - Bioengineering 2021Quote: The open reading frame for human SOX17 was PCR amplified using GoTaq Master Mix (Promega) from the PB-TRE3G-SOX17 plasmid (Table S5) ...
-
bioRxiv - Genetics 2022Quote: ... human POPDC1 and POPDC2 cDNA sequences were cloned into the pFC14K or pFC32K plasmids (Promega), which contain C-terminus sequences for HaloTag and NanoLuc tags ...
-
bioRxiv - Immunology 2024Quote: ... supernatant was removed for immediate analysis using the LumitTM HMGB1 (Human/Mouse) Immunoassay (Promega, #W6110). Luminescent samples were read in opaque white 96-well plates using a CLARIOstar plate reader (BMG Labtech).
-
bioRxiv - Cancer Biology 2024Quote: Lentivirus was produced by transfecting human embryonic kidney–293 T cells using FuGENE HD (Promega) as described (Gargiulo 2013) ...
-
bioRxiv - Bioengineering 2024Quote: ... and codon optimized human PAH exon 2-13 coding sequence with HiBit reporter tag (Promega) and cloned into plasmid backbone with Gibson assembly ...