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Citations for Promega :
251 - 300 of 870 citations for Anti Myc magnetic beads since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... antibodies before probing with horseradish peroxidase (HRP)-conjugated anti-rabbit (for H3) or anti-mouse (for anti-GFP and anti-TUB) as the secondary antibody (1:5000; Promega, Madison ...
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bioRxiv - Cell Biology 2020Quote: ... and finally eluted from the beads with an overnight digest performed at room temperature using Trypsin/LysC (Promega) and 0.02% ProteaseMax (Promega) ...
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Differential turnover of Nup188 controls its levels at centrosomes and role in centriole duplicationbioRxiv - Cell Biology 2019Quote: ... beads were washed twice with 600 µl of 50 mM ammonium bicarbonate (pH 8.0) and then trypsin (Promega) was added (200 ng in 200 µl of 25 mM ammonium bicarbonate ...
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bioRxiv - Genomics 2020Quote: ... Beads were resuspended in 50μL 100mM AMBIC and incubated overnight at 37°C with 400ng Trypsin (Promega V5280). Beads were supplemented with an additional 400ng Trypsin and incubated for a further 4 hours at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... Tryptic digestion was performed by incubating the beads with 1 µg MS-grade TPCK trypsin (Promega, Madison, WI) dissolved in 200 μl of 50 mM ammonium bicarbonate (pH 8.3 ...
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bioRxiv - Biochemistry 2020Quote: ... The diluted lysate was added to 100 µl of pre-equilibrated Magne® HaloTag® Beads (Promega, G7282) and incubated overnight on a rotator at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... the bead-bound protein was incubated with either 6.7 μM HaloTag-TMR or HaloTag-PEG-biotin ligand (Promega) for 15 minutes at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... the beads with bound recombinant proteins were blocked for 1 h at 4°C using reticulocyte lysate (Promega). Next ...
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bioRxiv - Cell Biology 2022Quote: ... The beads were collected by centrifugation at 8000 × g for 30 s or using a magnet separator (Promega), and washed three times with lysis buffer ...
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bioRxiv - Biophysics 2019Quote: ... the beads were incubated in a PBS solution containing 25 μg/mL of the HaloTag ligand O4 (Promega) for at least 4 h at constant rotation (room temperature) ...
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bioRxiv - Biochemistry 2022Quote: ... Elution was conducted in 3 beads volume of proteasome buffer containing 1 μL of TEV protease (Promega, PRV6101) for 1 hr at 37°C.
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bioRxiv - Plant Biology 2022Quote: ... the bead bound protein complexes were digested directly on beads by addition of 0.5 μg (1μg/μl) of trypsin (sequencing grade, Promega) in 50mM NaHCO3 buffer and incubated at 37°C for 18 h ...
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bioRxiv - Cell Biology 2024Quote: ... Tryptic digestion was performed by incubating the beads with 1 µg MS-grade TPCK trypsin (Promega, Madison, WI) dissolved in 200 μL of 50 mM ammonium bicarbonate (pH 8.3 ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested off the beads by adding 6 μL of 1 μg/μL sequencing grade Trypsin (Promega), diluted in 50 mM acetic acid ...
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bioRxiv - Biochemistry 2023Quote: ... Tryptic digestion was performed by incubating the beads with 1 µg MS-grade TPCK trypsin (Promega, Madison, WI) dissolved in 200 µL of 50 mM ammbic (pH 8.3 ...
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bioRxiv - Cell Biology 2020Quote: Proteins on beads were washed twice with 100 μL of 25 mM NH4HCO3 and we performed on-beads digestion with 0.2 μg of trypsine/LysC (Promega) for 1 h in 100 µL of 25 mM NH4HCO3 ...
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bioRxiv - Immunology 2021Quote: ... After 8 days of transfection mAbs were purified from culture supernatant using Magna Protein A beads (Promega, WI, USA).
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bioRxiv - Cancer Biology 2020Quote: ... proteins on the affinity pulldown beads were digested overnight at room temperature with 100 ng sequencing grade trypsin (Promega) and peptides analyzed by nano-liquid tandem MS as described in102 using an 75 μm × 150 mm analytical column (C18 ...
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bioRxiv - Plant Biology 2021Quote: ... On bead digestion was performed in 50 µl of ammonium bicarbonate solution containing 4 µl of Trypsin/LysC (Promega) solution and incubated at 37°C for 3 h in a thermomixer at 1,000 RPM ...
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bioRxiv - Molecular Biology 2021Quote: Proteins on beads were washed twice with 100 μl of 25 mM NH4HCO3 and subjected to on-beads digestion with 0.2 μg of trypsine/LysC (Promega) for 60 min in 100 μl of 25 mM NH4HCO3 ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant without beads was transferred to a new tube and digested with 150 ng trypsin (Trypsin Gold, Promega) in 1.5 µL 50 mM ammonium bicarbonate at 37°C for 5 hours ...
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bioRxiv - Genetics 2022Quote: ... Protein-bound beads were washed in 80% ethanol and proteins were digested off the beads by addition of 0.8ug sequencing grade modified trypsin (Promega) in 100mM Ammonium bicarbonate ...
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bioRxiv - Cancer Biology 2022Quote: ... The beads were finally added with 2.5 μl of 200 ng/µl Trypsin/Lys-C Mix (Cat. No. V5073, Promega) at 37°C with shaking overnight ...
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bioRxiv - Genomics 2019Quote: ... samples were transferred to pre-cooled 1.5mL screw-tubes with 300 µL glass beads and supplemented with 100 units of RNAse inhibitor (RNAsin plus, Promega). Cells were lysed using a FastPrep-24 shaker (6.0m/s for 15 seconds ...
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bioRxiv - Biophysics 2019Quote: ... the fluid chambers were exposed to a PBS pH 7.4 solution containing 1% glutaraldehyde to subsequently immobilize the reference beads (Spherotec) and the HaloTag ligand (Promega).
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bioRxiv - Genetics 2020Quote: ... beads were resuspended in 100 μL of ABC buffer and digested by adding 0.2 μg of trypsine/LysC (Promega) in 100 µL of ABC buffer for 1 hour at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... beads were incubated in 400 μl 50 mM ammonium bicarbonate containing 4 μl of sequencing-grade modified trypsin (Promega) at 37 °C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... Each sample was digested on beads in 100 µl of 50 mM ammonium bicarbonate with 1 µg trypsin (Promega) in spin cap tubes sitting in fresh mass-spectrometry grade Eppendorf tubes ...
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bioRxiv - Cancer Biology 2023Quote: ... and digested on-bead using filter-aided sample preparation47 with sequencing grade modified porcine trypsin (Cat. No. V5111, Promega). Nano-liquid chromatography tandem mass spectrometry (Nano-LC/MS/MS ...
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bioRxiv - Cell Biology 2023Quote: ... and digested on-bead using filter-aided sample preparation (Wiśniewski, Zougman, Nagaraj, & Mann, 2009) with sequencing grade modified porcine trypsin (Promega). Tryptic peptides were separated by reverse phase XSelect CSH C18 2.5 µm resin (Waters ...
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bioRxiv - Biophysics 2023Quote: ... In the first round of selection 400 nM of Xl-HAS-1-loaded nanodiscs diluted in the selection buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 1% BSA) was immobilized on streptavidin paramagnetic beads (Promega). Beads were washed three times in the selection buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... Beads were washed 6 times with 50 mM ammonium bicarbonate and then treated with TPCK-treated modified trypsin (Promega) for 16 hours at 37°C on an end-over-end rotator ...
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bioRxiv - Cancer Biology 2023Quote: ... beads were reconstituted in 5 μL 50 mM HEPES pH 8.0 buffer containing trypsin/rLys-C enzyme mix (Promega) at a 1:25 enzyme to protein ratio ...
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bioRxiv - Cell Biology 2021Quote: ... HRP-conjugated anti-rabbit and anti-mouse secondary antibodies (Promega) were used at dilution 1:10.000 in PBS-T supplemented with 5% instant nonfat dry milk for 1 hour at RT ...
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bioRxiv - Cell Biology 2021Quote: ... anti-ARC (SantaCruz Biotechnology, #sc-17839, anti-HaloTag (Promega, #G9211), anti-SNAP-tag (New England Biolabs ...
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bioRxiv - Plant Biology 2023Quote: ... Horseradish peroxidase-conjugated antibodies (anti-rabbit and anti-mouse; Promega) were applied followed by chemiluminescent ECL detection (Amersham ...
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bioRxiv - Microbiology 2022Quote: ... Indexed libraries were then cleaned using AMPure XP beads and quantified on the Quantus Fluorometer using the QuantiFluor ONE dsDNA System (Promega). Amplicons were purity-checked and sized on a TapeStation using D1000 ScreenTape System (Agilent) ...
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bioRxiv - Neuroscience 2021Quote: IP and pull-down probes of DG NSCs were subjected to on-bead digestion (Hubner et al., 2010) by trypsin (5 µg/ml, Promega) in 1.6 M Urea / 0.1 M Ammonium bicarbonate buffer at 27 C for 30 minutes ...
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bioRxiv - Genomics 2020Quote: ... A double-stranded DNA adapter with a T-overhang was ligated for 1 h at 20°C and the resulting products were bead purified (ProNex, Promega), eluted and then split into two identical aliquots ...
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bioRxiv - Biochemistry 2019Quote: ... The washed beads were resuspended in 200 µl of digestion buffer and incubated for 5 h with 1 µg of trypsin (Promega) at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... which were washed with a series of harsh washes before cross-linker reversion and on bead digest with Trypsin Lys-C (Promega). Peptides were then desalted and prepared for mass spectrometry ...
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bioRxiv - Cell Biology 2021Quote: ... Washed beads were re- suspended in 150 µl digestion buffer and incubated for 4 hours with 1 µg trypsin (Promega) at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... On-bead digestion was performed by overnight incubation at 37°C with 2 μg of mass spectrometry grade trypsin (Promega). The following morning an additional 2 μg of trypsin was added to each sample and incubated at 37°C for 4-6 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... mix was used at a concentration of 100 μg/μl for overnight “on-bead” digestion using LysC-trypsin mix (Promega)2,3 ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins on beads were digested overnight at 37°C by sequencing grade trypsin (12.5 μg/ml; Promega Madison, Wi, USA) in 20 μl of NH4HCO3 25 mmol/l ...
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bioRxiv - Genomics 2022Quote: ... Then the gel beads were washed with DPBS three times and 200 μL of proteinase K solution (1 mg/mL proteinase K [Promega, Madison ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was eluted from the beads using the elution buffer (1% SDS and 100 mM NaHCO3) and purified by PCR purification kit (Promega). The final products were analyzed by quantitative real-time PCR with the primers listed below:
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bioRxiv - Cell Biology 2020Quote: ... and the proteins were on-bead digested using 5 µl of Sequencing Grade Trypsin (100 ng/µl in 10 mM HCl, Promega). The digestion was carried out at 37°C over night ...
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bioRxiv - Molecular Biology 2019Quote: ... mRNA was isolated from ∼1 mg total RNA using oligo(dT) and streptavidin paramagnetic beads (PolyATtract mRNA Isolation Systems, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2020Quote: The cellular ATP levels were quantified in 100 μL ATP assay buffer or from 20-30 mg wound skin harvested in 200 μL assay buffer and lysed with the bead shocker following the manufacturer’s instructions (CellTiter-Glo luminescent ATP assay kit, Promega, Madison). The protein content from identical treated cells or tissues were determined by BCA Protein Assay kit for normalization (Young ...