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Citations for Promega :
2901 - 2950 of 3500 citations for Rat Bile Salt Activated Lipase CEL ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: The ability of T cells to lyse organoids was also measured using LDH-Glo™ Cytotoxicity Assay Kit (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was extracted from about 35 mg of tissue using the Maxwell™ 16 Tissue DNA purification kit (Promega) and total DNA concentrations were normalized to 20 ng/µl ...
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bioRxiv - Microbiology 2021Quote: ... Cells were harvested day 1 and day 5 post-infection using a CellTiter-Glo Luminescent Cell Viability Assay Kit (Promega) in accordance with the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... pancreatic sections were submitted to terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay using a DeadEnd Fluorometric TUNEL system kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Media was removed and cells were washed in PBS before re-suspension in 75 μL 1X Passive Lysis Buffer (PLB; Dual-Luciferase Reporter Kit, Promega). Cells were incubated in PLB for 15 min at RT and further homogenised by pipetting ...
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bioRxiv - Immunology 2020Quote: ... The plate reader was programmed to inject 40 μl of Luciferase Assay Substrate re-suspended in Luciferase Assay Buffer II and 35 μl of 1X Stop&Glo Reagent (Dual-Luciferase Reporter Kit, Promega) per well ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by a DNase I treatment and a subsequent reverse transcription with oligo d[T]18 using the GoScript RT kit (Promega). One-step RT-qPCR was performed using the SYBR FAST Mix optimized for LightCycler 480 (KAPA ...
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bioRxiv - Immunology 2020Quote: ... 293T cells were co-transfected with envelope-expressing plasmid and an env-deleted HIV-1 backbone plasmid (pSG3ΔEnv) using a FuGENE6 transfection kit (Promega Inc.). Cell supernatants containing pseudotyped viruses were harvested 48 h post transfection and used for infection in TZM-bl cells using DEAE-dextran (25 μg/ml ...
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bioRxiv - Microbiology 2021Quote: ... HEK293T cells were transiently transfected with plasmid DNA pHIV-1 NL4·3Δenv-Luc and Spike-Δ19-D614G by using Profection mammalian transfection kit (Promega Inc) following the instructions in the kit manual ...
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bioRxiv - Immunology 2020Quote: ... Total DNA from blood and urine (around 50 μL) was extracted using a Maxwell 16 automat with the Maxwell blood DNA and cell LEV DNA purification kits (Promega), respectively ...
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bioRxiv - Genomics 2021Quote: Total genomic DNA of bacterial isolates was extracted using the Wizard® Genomic DNA Purification Kit (Promega, Madison, WI, USA) as per the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Tris(2-carboxyethyl)phosphine (TCEP) and BCA protein assay kit were from Pierce and sequence-grade trypsin was from Promega. Rapigest and Sep-Pak C18 columns were from Waters and C18 Zip tips were from Millipore ...
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Glycogen Synthase Kinase 3 regulates the genesis of the rare displaced ganglion cell retinal subtypebioRxiv - Neuroscience 2021Quote: ... Apoptosis was detected by terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling (TUNEL) assays using in situ cell death detection kit (Promega). All images were acquired using a Zeiss LSM710 confocal microscope and Zen software (Zeiss).
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bioRxiv - Genetics 2020Quote: ... Colorimetric Cell Viability Kit I (WST-8 reagent, PromoKine, Heidelberg, Germany) and Caspase-Glo® 8 Assay (Promega, Madison, WI) were used for cell viability assay and caspase-8 assay ...
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bioRxiv - Microbiology 2022Quote: ... We extracted genomic DNA from whole blood using a Wizard® Genomic DNA Purification Kit (#A1120, Promega Corp., Madison, USA) and then performed IPATS-BLV ...
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bioRxiv - Developmental Biology 2022Quote: ... Luciferase assays were conducted 8 hours after stimulation using a dual luciferase kit (Dual-Luciferase® Reporter Assay System, Promega) according to manufacturer’s protocol.
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bioRxiv - Genetics 2022Quote: ... we added 25 uL Dulbecco’s PBS and 25 uL of reagent 1 from the Dual-Glo luciferase kit (Promega Inc.) and mixed well to lyse the cells ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA was purified from the gel fragments using the Wizard SV Gel and PCR Clean Up kit (Promega, Fitchburg, USA) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2022Quote: ... sequence was amplified from human genomic DNAs and used as in vitro transcription template in the reaction using Riboprobe System-T7 Kit (P1440, Promega) in the presence of 0.4μL 5-(3-Aminoallyl)-uridine-5’-triphosphate labeled with ATTO 680(Aminoallyl-UTP-ATTO-680 ...
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bioRxiv - Developmental Biology 2022Quote: ... the T7-VEE constructs were digested with Mlul and then in vitro transcribed using RiboMAX Large Scale RNA Production System Kit (Promega). The transcripts were 2’-O-methylated ...
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Zbtb14 regulates monocyte and macrophage development through inhibiting pu.1 expression in zebrafishbioRxiv - Developmental Biology 2022Quote: ... cells were harvested 48 hours after transfection and analyzed using the Dual Luciferase Reporter Assay Kit (Promega, Maddison, WI, USA), according to the manufacturer’s protocols ...
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bioRxiv - Genomics 2022Quote: ... A single colony was used to inoculate LB Broth and genomic DNA extracted from 1 mL using a Promega Wizard kit according to the manufacturer’s instructions (Promega, USA). DNA was quantified using the Qubit 3 and Nanodrop (Thermofisher ...
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bioRxiv - Physiology 2022Quote: ... cDNA was synthesized by using qScript cDNA Synthesis Kit (QuantaBio, Beverly, MA) and PCR was performed with GoTaq qPCR Master Mix (Promega) with HPRT as the control gene ...
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bioRxiv - Microbiology 2022Quote: ... were measured in 10 μL of each supernatant mixed with 10 μL 2X Renilla lysis buffer (RLB) by Renilla Luciferase Assay Kit (Promega) in white 96 well plates on a Berthold plate reader ...
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bioRxiv - Microbiology 2022Quote: ... Approximately 4 µg of the extracted material was then used as template to in vitro synthesized viral genomic RNA transcripts using the Ribomax T7 RNA transcription Kit (Promega) and Ribo m7G Cap Analogue (Promega ...
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bioRxiv - Molecular Biology 2022Quote: ... The firefly luciferase activity was detected in homogenates of organs by luminescence measurements using Synergy H4 hybrid reader (Bio-Tek) and Bright-Glo Luciferase Kit (Promega).
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bioRxiv - Synthetic Biology 2022Quote: ... and plasmids were extracted from individual cultures outgrown in LB/ampicillin (50 μg/mL) using the PURE Yield Plasmid Miniprep kit (Promega). Individual clones were screened and confirmed by Sanger sequencing at Macrogen-Europe B.V ...
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bioRxiv - Plant Biology 2022Quote: ... The DAP-seq gDNA library was prepared using KAPA HiFi HotStart ReadyMix with unique index primers. ZmEREB57 was fused to HaloTag using the kit from pFN19K HaloTag T7 SP6 Flexi Vecto (cat. no. G184A) (Promega). ZmEREB57 fused to HaloTag was expressed using the TnT SP6 High-Yield Wheat Germ Protein Expression System (L3260 ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was then synthesized using 2 μg of mRNA and a Transcriptase first-strand cDNA synthesis kit (Promega, Madison, WI). Real-Time qPCR to detect the indicated targets was performed on a LightCycler® 480 System (Roche Life Science ...
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bioRxiv - Microbiology 2023Quote: ... HEK293T cells were transiently transfected with plasmid DNA pHIV-1 NL4·3Δenv-Luc and Spike-Δ19-D614G by using profection mammalian transfection kit (Promega Inc.). After 48 hours ...
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bioRxiv - Genomics 2022Quote: ... DNA used for microarray was isolated from frozen cell pellets (3×106-7×106 cells) using the Maxwell RSC Cultured Cells DNA Kit on a Maxwell RSC 48 instrument (Promega). DNA was genotyped at the Children’s Hospital of Philadelphia’s Center for Applied Genomics using the Infinium Omni2.5-8 v1.3 BeadChip (Illumina ...
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bioRxiv - Neuroscience 2022Quote: Glucose uptake by cultured primary astrocytes was studied using a bioluminescent method with the Glucose Uptake Assay Kit (Promega, J1341) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Hybridization probes were generated by PCR amplifi cation using gene specifi c primers (Table S1) and radiolabeled with the Prime A Gene labeling kit (Promega) following the recommendations of the manufacturer.
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bioRxiv - Microbiology 2022Quote: Cell viability was measured using a commercially available kit [CellTiter 96 Aqueous One Solution Cell Proliferation Assay (Promega, Madison, USA)] ...
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bioRxiv - Cell Biology 2022Quote: Three major proteasome activities (chymotrypsin-like trypsin-like, caspase-like) were measured using the Proteasome-Glo Cell-Based Assay Kit (G1180, Promega) as described by the manufacturer ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were passaged every 3–4 days and regularly checked for mycoplasma infections using a GoTaq G2 Hot Start Taq Polymerase kit from Promega.
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bioRxiv - Microbiology 2022Quote: In vitro generated RNA transcripts above were used templates for in vitro translation assays using a Rabbit Reticulocyte Lysate kit (Promega) at the indicated concentrations ...
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bioRxiv - Zoology 2022Quote: ... The sperm suspension was then divided into two samples: one for determining ATP using a CellTiter-Glo Luminescent Cell Viability Assay kit (Promega) and the other for assessing DNA content (to calibrate the ATP content by the number of sperm cells ...
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bioRxiv - Molecular Biology 2022Quote: For Lytic endpoint measurements cells were seeded as mentioned above and NanoLuc abundance was determined via the Nano-Glo HiBit lytic detection kit (Promega) following manufacturers recommendations ...
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bioRxiv - Plant Biology 2024Quote: ... RNA extraction was performed using an RSC 48 RNA extraction robot (Maxwell) and the Maxwell RSC Plant RNA kit (Promega). Although the RNA yield for some of the 64 samples was low ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted using E.Z.N.A.® Plant RNA Kit (R6827-01, OMEGA) following the manufacturer’s instructions and treated with RNase-Free DNase (M6101, Promega) to remove genomic DNA contamination ...
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bioRxiv - Systems Biology 2024Quote: ... The assay was repeated three times and was conducted using the Caspase Glo® 3/7 assay kit (Promega, USA) based on the manufacturer’s recommendations.
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was isolated from 30 mg of ground plant tissue using the SV Total RNA Isolation System Kit (Promega) and its concentration and integrity were assessed using the RNA 6000 Nano Kit on a Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Microbiology 2024Quote: Lytic cell death was indirectly studied by measuring lactate dehydrogenase (LDH) release in cell culture medium using the CytoTox 96 nonradioactive cytotoxicity assay kit (G1780, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: Cell growth endpoint analyses were performed using CellTiter96 Aqueous Non-Radioactive Cell Proliferation MTS Assay kit (Promega, Madison, WI, USA) per manufacturer specifications ...
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bioRxiv - Microbiology 2023Quote: ... Total genomic DNA was extracted from the mid-log culture using the Wizard® Genomic DNA Purification Kit from Promega following the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was assayed starting at Day 1 for 6 days according to the CellTiter-Glo 3D kit (Promega G9683). For the antiandrogen response assay ...
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bioRxiv - Molecular Biology 2024Quote: ... The -1 PRF efficiency was assayed in cultured HEK293 as described previously (Kelly et al. 2020) using dual-luciferase reporter assay system kit (Promega). 24 hr post transfection ...
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bioRxiv - Microbiology 2024Quote: Genomic DNA was extracted from overnight cultures on GCB agar plates with appropriate components using Wizard Genomic DNA purification kit (Promega) following manufacturer instructions.
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bioRxiv - Molecular Biology 2024Quote: ... Tail DNA from offspring was genotyped for the presence of the BAC transgene by analyzing 100 ng of tail DNA with the GoTaq PCR reaction kit (Promega) according to the manufacturer’s instruction by adding forward primer 5′-GAGGCTCGGGTGCGGCAGCTC −3′ and reverse primer 5′-GTGCAGTGTGAGCACCTGCTGTC-3′ for PCR with an initial denaturation for 3 min at 95 °C ...