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Citations for Promega :
2901 - 2944 of 2944 citations for DIRAS Family GTP Binding RAS Like 1 DIRAS1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... The primer pairs used in dsRNA synthesis are shown in Supplemental Table 1.1 μg PCR product was used as the template for dsRNA synthesis utilizing the T7 Ribomax Express RNAi System (Promega, Madison, WI, USA). The dsRNA was isopropanol-precipitated ...
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bioRxiv - Genomics 2021Quote: ... We added tagmentation buffer (1x TD buffer, 0.015 µL TTE Mix V50 [Vazyme, #TD501], 0.625x protease inhibitor cocktail [Promega, #G6521], and 1 mM MgCl2) to reach a volume of 10 µL per well and then incubated the plates at 55 °C for 1 h ...
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bioRxiv - Systems Biology 2024Quote: ... the samples were diluted with 50 mM HEPES (pH 8.5) to reduce urea concentration to 2 M and further digested overnight with trypsin (Promega, 1:50 w/w) at RT ...
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bioRxiv - Developmental Biology 2024Quote: ... was amplified by PCR using tnnc1a-CDS primers (Table 1) and cloned into the pGEM®-T Easy Vector Systems (Promega, catalog #: A1360). A tnnc1a cDNA clone was confirmed by sequencing and used to make digoxigenin-labeled riboprobes with the Ambion™ MAXIscript™ SP6/T7 In Vitro Transcription Kit (Thermofisher ...
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bioRxiv - Bioengineering 2023Quote: ... Reverse transcription was performed at 70 °C for 5 min and 42 °C for 60 min with 1 µg total RNA using ImProm-II reverse transcriptase (Promega, Madison, WI, USA) and 250 ng oligo(dT)12-18 primers (ThermoFisher ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by 85℃ heat inactivation for 1 hour and PCR-based genotyping (GoTaq Green Master Mix, Promega, and C1000 Touch Cycler, Bio-rad). Tcf12 [34] ...
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bioRxiv - Developmental Biology 2024Quote: ... buffer (containing 10mM Tris pH 7.5, 10mM NaCl, 3mM MgCl2, 1% BSA, 0.1% Tween, 1mM DTT, 1U/ul RNaseIn from Promega, 0.1% NP40, and 0.01% Digitonin) and incubated on ice for 4 minutes ...
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bioRxiv - Microbiology 2024Quote: ... Six presumptive transconjugant colonies were randomly selected and grown overnight in 1 mL of LB prior to DNA extraction using the Wizard® Genomic DNA Purification kit (Promega, Madison, WI). The AMR genotypes of transconjugant colonies were determined by polymerase chain reaction (PCR ...
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bioRxiv - Immunology 2024Quote: ... cells was transfected with 250ng of each of 2 pX330 constructs (containing Cas9 and 1 sgRNA) and 500 ng of the plasmid template with FuGene HD transfection reagent (Promega Corporation, Madison, WI) in the recommended conditions ...
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Mucociliary Clearance Augmenting Drugs Block SARS-Cov-2 Replication in Human Airway Epithelial CellsbioRxiv - Microbiology 2023Quote: The cytotoxicity of all compounds (except ARINA-1 that interferes with the assay) was measured using the CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, Cat. # G1780), which measures LDH enzyme release ...
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bioRxiv - Microbiology 2023Quote: DNA from 1 ml of harvested phage stock was extracted using Wizard PCR DNA Purification Resin and Minicolumns (both Promega, Madison, WI, USA) as described previously [49] ...
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bioRxiv - Immunology 2023Quote: ... 1.5 μL of N2 primers and probe (2019-nCov CDC EUA Kit, Integrated DNA Technologies) and 10 μl of GoTaq 1-Step RT-qPCR (Promega, Madison, WI, USA). Thermal cycling was performed at 50°C for 15min for reverse transcription ...
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bioRxiv - Cell Biology 2023Quote: ... 35S-methionine-labeled Ctb or its derivatives were produced in coupled in vitro transcription and translation reaction (IVTT, at 30 °C for 1 h) using TNT Quick Coupled Transcription/Translation System (cat#L1170, Promega, Madison, WI, USA). Prey proteins were diluted in binding buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Genomics 2023Quote: ... The resulting DNA was resuspended in 1× IDTE buffer (Integrated DNA Technologies, Coralville, IA, USA) and quantified using the QuantiFluor One dsDNA kit (Promega, Madison, WI, USA). The HiFi reads were obtained using the SMRT Cell 8M and the PacBio Sequel II instrument ...
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bioRxiv - Biochemistry 2023Quote: ... each sample was hydrolyzed to 100 µg of protein in a 50: 1 ratio using trypsin (trypsin Gold, MassSpec Grade, Promega, Fitchburg, WI, USA). After enzymolysis at 37 for 14 h ...
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bioRxiv - Systems Biology 2023Quote: ... at the ratio of 1:50 for 3 h at 37°C and then using trypsin (sequencing grade modified trypsin; Promega, Fitchburg, WI, USA) at the ratio of 1:50 at 37°C overnight (for 15 h to 18 h ...
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bioRxiv - Biochemistry 2023Quote: ... and 20 µL of 1:1000 (diluted in phenyl red-free DMEM with 4% FBS) HaloTag™ NanoBRET™ 618 Ligand (Promega G9801) was added to each well ...
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bioRxiv - Microbiology 2023Quote: ... As a qPCR standard the recombinant plasmid pBELX-1 (Bellanger et al., 2014) was extracted using the Wizard plus SV Miniprep DNA Purification System (Promega, Madison, WI, USA) according to the manufacturers’ instructions and linearized by restriction enzyme BamHI (Promega ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was discarded and pelleted nuclei were resuspended in 3 ml blocking buffer (1xPBS [Sigma-Aldrich, P4417-50TAB, US], 1% BSA [Sigma-Aldrich, A2153-10G, US], 0.2 U/µl RNAsin Plus RNase inhibitor [Promega, N2615, US]).
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bioRxiv - Cell Biology 2024Quote: ... membranes were washed 3 times for 5 minutes in TBST or TBST-XL for mucin-2 and incubated with anti-mouse IgG HRP Conjugate (1:10000, Promega, Cat no. W4021) or anti-rabbit IgG HRP Conjugate (1:10000 ...
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bioRxiv - Biochemistry 2024Quote: ... and tumors were briefly washed with FluoroBrite DMEM prior to submerging in 200 µL furimazine-FluoroBrite (diluted 1:200 from the Promega Nano-Glo kit). L713F-nLuc signals were then immediately measured using nLuc-specific filter settings (470 ± 40 nm) ...
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bioRxiv - Neuroscience 2024Quote: ... For experiments cells were plated onto poly-D-lysine coated glass coverslips supplemented with 1 μM all-trans retinal and transfected using FuGENE® HD (Promega, Madison, USA) the next day ...
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bioRxiv - Biophysics 2020Quote: ... samples were blocked in 20% normal goat serum (NGS) for 1 h and incubated with the HaloTag® Biotin Ligand (Promega, 200-fold dilution) in PBS supplemented with 5% NGS overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... The gel piece was vacuum dried and treated with trypsin (15 ng/μl in 25 mM ammonium bicarbonate, 1 mM CaCl2; Promega or Roche sequencing grade) at 37°C for 16 hours ...
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bioRxiv - Cell Biology 2020Quote: ... samples were blocked in 20% normal goat serum (NGS) for 1 h and incubated with the HaloTag® polyclonal anti-rabbit (Promega, 100-fold dilution) in PBS supplemented with 2% NGS overnight at 4°C ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... 24h prior to imaging cells were co-transfected with human PEX31-42-GFP-Halo and either human HAP1-mCherry-eDHFR or mouse BICD21-572-mCherry-eDHFR using FuGENE 6 (Promega; 1 µg total DNA) and 48h prior to imaging with control siRNA using Lipofectamine RNAiMAX (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2024Quote: ... and pellets were washed twice with ice-cold ethanol followed by overnight incubation at 37°C in 100 mM ABC containing 1 mM CaCl2 and trypsin (Sequencing grade, modified, Promega Co, Madison, WI, USA). Digested proteins were evaporated and each sample was freed from salts and buffers by solid-phase extraction on C18 resin using cartridges prepared in-house ...
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bioRxiv - Microbiology 2023Quote: ... Samples were diluted in 50 mM ammonium bicarbonate and normalized to 50 μg protein for overnight LysC/trypsin digestion (Promega, protein:enzyme ratio, 50:1). Trifluoroacetic acid (TFA ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary HRP-conjugated antibodies were added at a dilution of 1:10,000 in 5% milk in TBST and incubated for 60 minutes at room temperature (Anti-Mouse: Promega #W402B; Anti-Rabbit: Promega #W401B). The Pierce SuperSignal West Pico PLUS chemiluminescent substrate (Thermo Scientific #34577 ...
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bioRxiv - Cell Biology 2023Quote: ... pH 8.5 to a final urea concentration of 2 M for Trypsin/Lys-C based overnight protein digestion at 37 °C (40 µg of protein used for global proteomics and the remainder for phosphoproteomics, 1:70 protease: substrate ratio, Mass Spectrometry grade, Promega Corporation, Cat No: V5072.) Digestions were acidified with trifluoroacetic acid (TFA ...
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bioRxiv - Immunology 2023Quote: ... stable HEK293 EBNA cell lines were generated using the sleeping beauty transposon system.91 Expression constructs were co-transfected with a transposase plasmid (10:1) into the HEK293 EBNA cells using FuGENE® HD transfection reagent (Promega GmbH, Madison, USA) in DMEM/F12 supplemented with 6% FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... diluted with ABC to a final concentration of 1 M urea and digested using trypsin (Promega, V5113; 3 μg/mL, 37°C, 16 h). After digestion ...
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bioRxiv - Microbiology 2023Quote: ... the presence of other main circulating arboviruses was evaluated by RT-qPCR using GoTaq® 1-Step RT-qPCR System (Promega, Madison, WI, USA). Specific diagnosis primers were used for Zika [30] ...
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bioRxiv - Biochemistry 2024Quote: ... the amplified PCR products were digested using the corresponding restriction enzymes (Supplementary Table 1) and then ligated into similarly digested pYES2 using LigaFast Rapid Ligation System (Promega Corporation, Madison, WI, USA) following the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2020Quote: ... the RNA-bound beads were washed four times in 900 μL of 0.35 M KCl (10 mM HEPES [pH 7.4], 350 mM KCl, 10 mM MgCl2, 1% NP40, 2 mM DTT, 100 U/mL RNasin® Ribonuclease Inhibitors [Promega, N2111], and 100 μg/mL cycloheximide). During the final wash ...
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bioRxiv - Cell Biology 2021Quote: ... 30 min at 60°C) an alkylation (IAA 0.5M, 30 min RT) microtubule-associated protein enriched fractions were digested using trypsin (Gold, Promega, 1 μg / sample, overnight at 30°C). Peptide clean-up was done using OMIX C18 (Agilent ...
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bioRxiv - Neuroscience 2020Quote: ... the RNA-bound beads were washed four times in 900μL of 0.35M KCl (10mM HEPES [pH 7.4], 350 mM KCl, 10 mM MgCl2, 1% NP40, 2 mM DTT, 100 U/mL RNasin® Ribonuclease Inhibitors [Promega, N2111], and 100 μg/mL cycloheximide). During the final wash ...
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bioRxiv - Neuroscience 2020Quote: ... the RNA-bound beads were washed four times in 900μL of 0.35M KCl (10mM HEPES [pH 7.4], 350 mM KCl, 10 mM MgCl2, 1% NP40, 2 mM DTT, 100 U/mL RNasin® Ribonuclease Inhibitors [Promega, N2111], and 100 μg/mL cycloheximide). During the final wash ...
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bioRxiv - Microbiology 2020Quote: ... alkylation was performed using 20 mM 2-chloroacetamide at room temperature in HEPES buffer for 30 min under exclusion of light. Samples were prepared according to the SP3 protocol (Hughes et al. 2019) and trypsinized (sequencing grade, Promega, enzyme to protein ratio 1:50) overnight at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Each organ was homogenized with a lysis solution containing a 1:5 dilution of Luciferase Cell Culture Lysis 5X Reagent (Promega, Madison, WI, USA, cat. no. E1531) in Millipore water supplemented with 1 % (v/v ...
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bioRxiv - Microbiology 2020Quote: ... for 3 hours before being diluted with 5 volumes of 40 mM NH4HCO3 and digested overnight with sequencing grade modified trypsin (Promega, Madison, WI, USA; 1/50 w/w). Digested samples were acidified to a final concentration of 0.5% formic acid and desalted with 50 mg tC18 Sep-Pak columns (Waters corporation ...
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bioRxiv - Biochemistry 2021Quote: ... The dried resin was resuspended with 50 mM Tris pH 7.5 (2x resin volume) and 200 ng of trypsin was added (Promega; made up to 100 ng·μL-1 in H2O), the samples were digested overnight shaking at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Light signals derived from reconstituted HiBiT/LgBiT association were measured using Tristar multiwell luminometer (Berthold Technology, Bad Wildbad, Germany) for 2.5 seconds by adding 20 µl of Nano-Glo® substrate (Promega Inc, WI, USA; diluted 1:40 in PBS). Mock-infected cells served as controls to determine basal luciferase activity for obtaining normalized relative light units in infected samples ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then incubated at 37 °C in tissue culture incubator for 4 hours or 24 hours before measuring reconstituted HiBiT/LgBiT activity using a Tristar multiwell Luminometer (Berthold Technology, Bad Wildbad, Germany) for 2.5 seconds by adding 20 µl of Nano-Glo® substrate (Promega Inc, WI, USA; diluted 1:40 in PBS). Individual unmixed populations of cells were treated identically and served as controls to determine basal luciferase activity to obtain normalized relative light units ...