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Citations for Promega :
2851 - 2900 of 3110 citations for Recombinant Human High Mobility Group Box 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Real-Time PCR Diagnostic Panel17 and a protocol for quantifying the SARS-CoV-2 subgenomic E gene RNA (E SgRNA)18 using the GoTaq® Probe 1-Step RT-qPCR System (Promega). For quantification of SARS-CoV-2 using the nCoV assay ...
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bioRxiv - Genomics 2022Quote: ... Membranes were washed in TBST and probed with anti-mouse HRP secondary antibody (Promega; 1:2,000 in 5% BSA in TBST) for 1 hour at room temperature followed by development in ECL (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: Cell pellets were thawed in Lysis buffer (50mM Tris pH 7.5, 150mM NaCl, 1% NP-40 supplemented with protease inhibitor cocktail (Promega cat #G6521)) and incubated on ice for 15min ...
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bioRxiv - Cancer Biology 2022Quote: ... Pellets were thawed with lysis buffer (50mM Tris pH 7.5, 150mM NaCl, 1% Triton X100, 1mg/ml Na-deoxycholate) supplemented with protease inhibitor (Promega cat #G6521). The bait is covalently bound through HaloTag to the beads and therefore cannot be detected by blotting.
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bioRxiv - Microbiology 2021Quote: ... Virus-mAb-containing media was then aspirated from cells and 100 μL of a 1:4 dilution of Bio-glo (Promega, G7940) in PBS was added to cells ...
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bioRxiv - Neuroscience 2020Quote: ... total RNA was placed in 4 µl of lysis buffer (0.1% vol/vol Triton X-100, 2.5 mM dNTPs, 2.5 µM oligo-dT, 1 U/µl Promega RNasin Plus RNase inhibitor). Reverse transcription was performed followed by cDNA amplification ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Real-Time PCR Diagnostic Panel17 and a protocol for quantifying the SARS-CoV-2 subgenomic E gene RNA (E SgRNA)18 using the GoTaq® Probe 1-Step RT-qPCR System (Promega). For quantification of SARS-CoV-2 using the nCoV assay ...
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bioRxiv - Cell Biology 2021Quote: ... and 40 μg of proteins/sample was loaded onto S-trap columns (Protifi) and digested overnight with 1 μg sequencing grade modified trypsin (Promega, V5111) at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were digested overnight at room temperature with a 1:50 ratio of sequencing grade modified trypsin (0.4ug, V511A, Promega, Fitchburg WI) in 130ul TEAB (0.05M Triethylammoniumbicarbonate in water) ...
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bioRxiv - Biochemistry 2021Quote: ... and the “on-bead-digestion” plate (100 μl 20 mM Tris pH 8.5, Sigma-Aldrich 10708976001, 1 μg/mL LysC, Wako 129-02541, 2 μg/mL Trypsin, Promega V5111). The programmed sequence is ...
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bioRxiv - Biochemistry 2020Quote: ... The RNA was added to the 100 µl SA Sepharose slurry together with 1 µl RNasin Plus RNase inhibitor (40 U/µL, Promega, N261A). 10 µl of the supernatant was saved for extraction of input RNA using TRIzol (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... then diluted 1:5 with nuclease-free water before 2 µL was used as template in a 10 µL GoTaq 1-Step RT-qPCR (Promega, A6020) reaction (69) ...
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bioRxiv - Systems Biology 2022Quote: ... The urea concentration was then diluted to 1 M with 50 mM ABC and the proteins digested with trypsin (Promega, V511c) at 1:100 mass-ratio at 37 °C overnight.
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bioRxiv - Immunology 2022Quote: ... Samples were diluted two-fold with 100 mM of triethylammonium bicarbonate (TEAB) and proteins were digested during 3 hours with 1 µg of trypsin (Promega V5111) and 1 µg of LysC (Wako 129-02541 ...
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bioRxiv - Immunology 2022Quote: ... HEK293 cells were transfected with the ORF8 plasmid together with the transposase coding plasmid (1/10) with Fugene HD (Promega, Germany) and after one day selected with 3 µg/ml puromycin for three days ...
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bioRxiv - Cancer Biology 2022Quote: ... These cells were transfected with the pcDNA6.2-GW /EmGFPmiR plasmids containing miR sequences (miR-NRF2 #1, #2, #3, #4 and miR-ctl 79 using the FUGENE HD transfection reagent (Promega #E2311). Seven days later GFP positive cells were isolated by flow cytometry (Biorad S3e cell sorter) ...
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bioRxiv - Cancer Biology 2022Quote: ... 20µg of chromatin was subjected to buffer exchange through SP3 protein clean up protocol (87) and resuspended in AmBic-10mM DTT before proteins digestion with 1:50 trypsin (Promega V5280) for 18h ...
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bioRxiv - Systems Biology 2019Quote: ... The samples were then diluted with 0.1 M NH4HCO3 to a final concentration of 1 M urea and the proteins were digested with sequencing-grade porcine trypsin (Promega, Switzerland) at a final enzyme:substrate ratio of 1:100 (w/w) ...
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bioRxiv - Microbiology 2019Quote: The following commercial antibodies and dilutions were used for immunofluorescence staining: rabbit polyclonal antibody to HaloTag (Promega; G9281; 1:500 dilution), mouse monoclonal antibody to lamin A/C (Cell Signaling ...
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bioRxiv - Physiology 2020Quote: ... Larvae with intestinal tracts clearly outlined with Nile Red dye were embedded in 1% low melting point agarose (Promega, Madison, WI) in E3 supplemented with 0.16 mg/mL tricaine in 6-well glass bottom plates (#1.5 high performance cover glass ...
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bioRxiv - Microbiology 2020Quote: ... After 24 hours of growth luciferase activity was visualized by spraying one spray of 1:100 reconstituted NanoGlo Luciferase substrate (Promega N1110) per agar plate using a disposable spray flask ...
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bioRxiv - Microbiology 2021Quote: ... 2 mL aliquots of phage lysate with a titer of approximately 109 PFU mL-1 was digested with DNase I (Thermo, CA, USA) and RNase A (Promega, USA) to remove any residual bacterial genomic DNA and RNA according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... using 1 μg of total Arabidopsis or cauliflower mtRNA and 250 ng of a random hexanucleotide mixture (Promega, Cat no. C1181) and incubated for 50 min at 50°C ...
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bioRxiv - Neuroscience 2021Quote: ... reverse transcription of viral RNA followed by quantitative PCR was performed with GoTaq® Probe 1-Step RT-qPCR System (Promega) on a 7500 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Amplification reactions were run in a 20 μL volume using the GoTaq 1-Step RT-qPCR System (Promega, Madison WI, USA) and the recommended manufacturer’s instructions as described in Cervera et al ...
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bioRxiv - Cell Biology 2021Quote: ... 2 μl) were mixed with the master mixes containing 1 μl primers (10 μM each) and reagents of GoTaq G2 (Promega #M7845) and the final volume was 25 μl ...
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bioRxiv - Biochemistry 2021Quote: ... The membrane was washed with TBS-T and imaged using 500 µl of 1:500 NanoGlo substrate (Promega, catalogue number: N1120) diluted in 10 mM sodium phosphate buffer pH 7.0.
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bioRxiv - Developmental Biology 2022Quote: ... for the duration of imaging (approximately 2 minutes per embryo) with the yolk supported in a shallow well of solidified 1% low-melting agarose (Promega, V2111). Adult fish were photographed using a Panasonic DMC GX7 camera with a Panasonic Lumix G 20mm pancake lens ...
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bioRxiv - Microbiology 2020Quote: ... samples were heated at 80 °C for 15 min and cooled to RT before addition of 1 μg of trypsin (Promega, V511A). Samples were digested at 37 °C overnight ...
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bioRxiv - Cancer Biology 2020Quote: ... Viability measurements were read after 1 hour (for baseline) and after 24-96 hours with RealTime-Glo™ MT Cell Viability Assay (Promega).
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bioRxiv - Cell Biology 2020Quote: ... and Gaussia luciferase was assayed from 1:40 diluted cell culture supernatant using either the Stop & Glo reagent from the DualLuciferase® Reporter Assay System (Promega) or BioLux® Gaussia Luciferase Assay Kit (New England Biolabs ...
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bioRxiv - Immunology 2021Quote: ... RNA concentration was determined by NanoDrop 1000 Spectrophotometer (ThermoScientific).1 µg of total RNA was reverse transcribed into cDNA with M-MLV Reverse Transcriptase 200 units (Promega, #M1701), Random Primer 0.5 μg (Promega ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were diluted 8 times with 0.1M ammonium bicarbonate and sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin) was added ...
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bioRxiv - Neuroscience 2022Quote: ... First strand of cDNA was synthesized from 0.5-1 μg of total RNA using Im-Prom-II system (Promega, Cat. A3800). Real-Time PCR was performed using iTaq qPCR master mix according to manufacturer’s instructions (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were alkylated using 15 mM chloroacetamide at 37° C for 30 min and further digested using sequencing-grade modified trypsin (1/50 w/w, ratio trypsin/protein; Promega, USA) at 37° C for 12 hours ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... The urea concentration was reduced to <2M and the protein sample was digested overnight at 37°C with 1:100 Trypsin Gold (Promega V5280). The following day ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA (1 μg) was reverse-transcribed using oligo(dT)18 and M-MLV reverse transcriptase II (Promega, Fitchburg, WI, USA).
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bioRxiv - Plant Biology 2022Quote: ... Membranes were washed 3 × 10 min in TBS-T (0.1%) before 1hr incubation in secondary antibodies (anti-rabbit or anti-mouse HRP or AP conjugate (Promega; 1: 5.000)) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... media was removed and replaced with 50 µL per well 0.01 mM Furimazine or NanoGlo® Lytic Buffer (diluted 1 in 500, Promega, UK). After a 10-minute shaking incubation protected from light ...
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bioRxiv - Plant Biology 2022Quote: ... 6.5 μl of 1:100 diluted cDNA per reaction were used with the GoTaq® qPCR Mastermix (Promega GmbH, Madison, USA) in a total volume of 15 μl ...
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bioRxiv - Physiology 2022Quote: ... Protein concentration in the supernatant was measured using a NanoDrop and 1:200 (w/w) of trypsin (Promega, Mass Spectrometry grade) was added to 200 µg of sample ...
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bioRxiv - Synthetic Biology 2022Quote: To measure luciferase activity on plaque containing LB-agar plates, 5 μl of the diluted (1:50, in PBS) NanoLuc® luciferase substrate 2-furyl methyl-deoxy-coelenterazine (Furimazine; Promega) were dropped onto the respective plaques ...
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bioRxiv - Microbiology 2022Quote: ... RNA samples and the negative extraction controls were screened in duplicate to investigate the presence of YFV RNA by RT-qPCR (GoTaq Probe 1-Step RT-qPCR System-Promega, USA), with primers and probes targeting the 5’-noncoding regions of the YFV genome (39) ...
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bioRxiv - Cell Biology 2024Quote: ... After PCR pre-amplified cDNA were clean-up using 22% home-made PEG beads at a ratio of 0.7 beads:1 sample before concentration quantification using QuantiFluor® dsDNA System (Promega, #E2671). cDNA was normalised and diluted to 100pg µl-1.
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bioRxiv - Plant Biology 2024Quote: ... Reverse transcription was achieved from 1 𝜇g of total RNA with M-MLV reverse transcriptase (RNase H minus, Point Mutant, Promega, USA) using an anchored oligo(dT)20 primer ...
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bioRxiv - Bioengineering 2024Quote: ... were taken for overnight digestion with mass spec grade Trypsin/Lys-C Mix (Promega, Madison, WI, cat. No. V5071, 1:20). Samples were reduced with TCEP (final concentration 5 mM ...
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bioRxiv - Biochemistry 2024Quote: ... when the temperature was dropped to 22°C and isopropyl β-d-1-thiogalactopyranoside (IPTG) and L-rhamnose (Promega, Madison, WI) was added at 0.2 mM and 0.1% (w/v ...
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bioRxiv - Biochemistry 2024Quote: HEK293T cells were transfected in a 6-well plate with 1 μg ADGRG6 DNA and 3 μL transfection reagent Fugene 6 (Promega, PRE2693) at 60-70% confluency and incubated for 48 h ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected the following day using 0.6–1 µg of plasmid and Viafect reagent (Promega E4981, 6 µl/µg plasmid). The medium was exchanged the following day to regular culture medium ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were then washed 3x with TBS-T and incubated for 2 hours at room temperature with either anti-rabbit (1:5000, Promega W4011) or anti-mouse HRP (1:5000 ...