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Citations for Promega :
2751 - 2800 of 5641 citations for ssc mir 411 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... or the region between abaR and the abaI (for the abaI fusion) were amplified by PCR and ligated in pGEM®-T Easy using the pGEM®-T Easy Vector System (Promega). The resulting plasmids and the promoterless luxCDABE operon were digested with KpnI and BamHI and ligated in order to introduce the lux operon downstream of the predicted promoter of abaM or abaI ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 µl of cDNA per sample were used and qRT-PCR was performed using the GoTaq® qPCR Master Mix (Promega, A6001) on a LightCycler 96 (Roche) ...
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bioRxiv - Cancer Biology 2020Quote: ... Recombination was verified by PCR with primers listed in Supplementary table 5 and genomic DNA isolated with the ReliaPrep™ gDNA Tissue Miniprep System (Promega, A2052) and peqGOLD Tissue DNA Mini Kit (Peqlab ...
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bioRxiv - Neuroscience 2022Quote: ... and mouse cholecystokinin (CCK, 3-688 of NM_031161) were PCR-amplified and subcloned into the pGEM-T Easy vector (Promega, Fitchburg, WI, USA). Digoxigenin (DIG)-labeled anti-sense probes were prepared using DIG RNA Labeling Mix (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... The PCRs were performed in a final volume of 20 μl of amplification mix containing 1 U of GoTaq polymerase (Promega, Madison, WI), 1× GoTaq buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... RiMCO1 and RiMCO3 full-length cDNAs were obtained by PCR amplification of the genes cloned in pGEM-T easy vector (Promega, Madison, USA), using the corresponding primer pairs (Table S1) ...
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bioRxiv - Cancer Biology 2022Quote: Synthesized cDNA as described above was used to measure levels of unspliced and spliced XBP1mRNA were measured by regular PCR performed with GoTaq Green Master Mix (Promega cat#: M7123) on a T100 Thermo Cycler (Bio-Rad cat# ...
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bioRxiv - Synthetic Biology 2022Quote: ... Amplified DNA products were purified using the Wizard® SV Gel and PCR Clean-Up System (Promega GmbH, Germany, Art. No. A9282). In all agarose gels ...
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bioRxiv - Physiology 2019Quote: An aliquot of cDNA obtained by reverse transcription was amplified in a PCR reaction with GoTaq Green Master Mix (Promega, Milan, Italy). For each pair of primers ...
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bioRxiv - Physiology 2019Quote: ... +1 corresponding to start of the first exon) was amplified by PCR and cloned into the pGL4.21-Luc vector (Promega, Madison, WI, USA). Systematic promotor truncations were generated using the Q5 Site-Directed Mutagenesis Kit according to manufacturer’s instructions (New England Biolabs ...
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bioRxiv - Genomics 2019Quote: ... All PCR products were checked on 2% agarose gels and then cleaned with the Wizard SV Gel and PCR Clean-up System from Promega (Madison, Wisconsin) and sent off for Sanger-sequencing along with the amplification primers to Molecular Cloning Laboratories (San Francisco ...
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bioRxiv - Immunology 2022Quote: ... that cover the promoter region and proximal portion of the first exon of the gene was PCR amplified with GoTaq DNA Polymerase (Promega, Madison, Wisconsin) from bisulfite-converted DNA using the primer listed in Table S3 ...
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bioRxiv - Physiology 2022Quote: ... agarose gel and the appropriate bands were cut out and purified according to the Wizard SV Gel and PCR Clean-Up System (Promega, Fitchburg, USA). A 20 min A-tailing step (72 °C ...
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bioRxiv - Genetics 2024Quote: ... The qRT-PCR reactions were conducted in a 10 μL volume using the GoTaq qPCR Master Mix chemistry (Promega, Madison, Wisconsin, USA) and a CFX384 Touch Real-Time PCR Detection System (BioRad ...
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bioRxiv - Molecular Biology 2022Quote: ... and purified using the Wizard SV Gel and PCR Clean-R19 purification systems following the instructions by the manufacturer (Promega, Madison, USA). The positive PCR amplicons were bidirectionally sequenced and gathered using BioEdit software (Hall ...
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bioRxiv - Neuroscience 2023Quote: ... the 5’-flanking region of mouse TrkA gene (79799-80637 of AC161454.3) was PCR-amplified from the genomic DNA of C57BL6J mice and subcloned into the pGL2-basic vector (Promega, Fitchburg, WI, USA). The following primers were used for PCR amplification ...
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bioRxiv - Microbiology 2023Quote: ... The 650 bp positive fragment was purified by Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, Madison, WI, USA).
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bioRxiv - Microbiology 2023Quote: ... The 1800 bp positive amplicons was purified using Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, Madison, WI, USA).
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bioRxiv - Cancer Biology 2023Quote: ... Promoter fragments corresponding to wild type and mutant p53 target genes were amplified by standard PCR using DNA from healthy donors and subsequently cloned into the pGL4.2 basic vector (Promega Corporation, Madison, USA) for promoter-luciferase assays ...
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bioRxiv - Immunology 2023Quote: ... 4 µl of cDNA per sample were used and qRT-PCR was performed using the GoTaq® qPCR Master Mix (Promega, #A6001) on a LightCycler 96 (Roche) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The identity of PCR products was verified by Sanger sequencing of amplicons cloned using the pGEM-T cloning system (Promega, Madison, WI) as per manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... The first-strand cDNA from a colonic sensory neuron was used as template in a PCR reaction containing 1×GoTaq reaction buffer (Promega Madison, WI), 20 mM outer primers ...
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bioRxiv - Neuroscience 2020Quote: ... DNA and RNA were extracted from formalin-fixed paraffin-embedded (FFPE) tissue using the Maxwell 16 FFPE Plus LEV DNA Kit and Maxwell 16 LEV RNA FFPE Purification Kit (Promega, Madison, WI, USA), according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... membrane integrity (CytoTox-OneTM homogeneous membrane integrity assay kit: lactate dehydrogenase (LDH release) and caspase activity/ apoptosis (Caspase-Glo® 3/7 assay system kit) (all from Promega, WI, USA), as described previously17 (see Supplementary data for detailed description).
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bioRxiv - Biophysics 2021Quote: ... The 2725-nt RNA molecules were synthesized by polymerase chain reaction (PCR) amplification of the plasmids followed by in vitro transcription using the T7 RNA polymerase (Promega, Fitchburg, WI, USA). The RNAs contain a 1206-nt upstream sequence ...
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bioRxiv - Cell Biology 2020Quote: ... Two microliters aliquots of the generated cDNA was amplified in 50 μl of PCR reaction mixture containing 2X GoTaq® green master mix (Promega, Madison, WI), 100 nM of each primer and ddH2O ...
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bioRxiv - Developmental Biology 2021Quote: ... Probes are synthetized from a PCR product or a linearized plasmid (see Table 2) with the T7 or SP6 RNA polymerase (Promega, P4074 or P4084) in presence of DIG-UTP nucleotides ...
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bioRxiv - Cell Biology 2019Quote: An attR3/attR4 MultiSite Gateway cassette was PCR-amplified from pK7m34GW (Karimi et al., 2005) using GoTaq DNA Polymerase (Promega UK, Southampton, UK) with primers NdeI-R3 and NdeI-R4 (Table S2) ...
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bioRxiv - Molecular Biology 2019Quote: The putative LINC00662 binding regions within the ELK4 gene were amplified by PCR and cloned into downstream of pmirGLO dual-luciferase vector (Promega, Madison, WI, USA) to form the wide-type plasmid (ELK4-3’UTR-Wt ...
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bioRxiv - Neuroscience 2020Quote: ... 40ng of each cDNA preparation (a quantity calculated from the RNA concentration on the assumption that reverse transcription of RNA into cDNA was complete) was used to perform PCR using GoTaq® DNA polymerase (Promega, Madison, USA). Each 25μL PCR reaction contained 0.2mM of deoxyribonucleotide triphosphates (dNTPs) ...
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bioRxiv - Developmental Biology 2020Quote: ... The purified PCR products were used as templates to synthesize dsRNA using the T7 RiboMAX Express RNAi System (Promega Corporation, Madison WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... Barcodes were amplified using forward primer MWV 486 and a 5’-biotinylated reverse primer MWV 358 (Table S1) using standard PCR conditions with Gotaq G2 flexi DNA polymerase (Promega, Leiden, the Netherlands). The biotinylated PCR products were then hybridised to a pool of MagPlex-TAG microspheres (Table S3 ...
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bioRxiv - Physiology 2022Quote: Capped cRNAs of the two Atlantic salmon PepT2 were synthesized by in vitro transcription using T7 RNA polymerase from cDNAs in pSPORT1 linearized with NotI and purified with Wizard SV Gel and PCR clean-up system (Promega Italia, Milan, Italy). The purified cRNAs were quantified by NanoDropTM 2000 Spectrophotomer (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting PCR reaction was then cloned into pGEM T-Vector system and selected for using the XGal/IPTG system (Promega, Madison, WI, USA). Sanger sequencing was performed at the University of Pennsylvania Genomic Analysis Core with the SP6 promoter/primer ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR products were purified by agarose gel electrophoresis and extracted from the gel using the Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA). Purified PCR products were cloned into a pCR2.1-TOPO vector (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... Supplementary Table 4) and DNA sequencing of the products following clean-up (Wizard SV Gel and PCR Clean-Up System, Promega, Southampton, Hampshire, UK) using dye terminator chemistry (Eurofins ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Full-length coding sequences were cloned into the transcription vector pTB-207 and RACE-PCR product were cloned into pGEM-T (Promega, Madison, WI, USA). Eurofins Genomics (Luxembourg ...
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bioRxiv - Microbiology 2019Quote: ... The primer pairs used in dsRNA synthesis are shown in Supplemental Table 1.1 μg PCR product was used as the template for dsRNA synthesis utilizing the T7 Ribomax Express RNAi System (Promega, Madison, WI, USA). The dsRNA was isopropanol-precipitated ...
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bioRxiv - Microbiology 2021Quote: ... The linearized vector and the slpMh-6x His fragment were gel-purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, Mannheim, Germany) and assembled via isothermal in vitro ligation according to Gibson et al ...
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Highly Versatile, Non-Invasive Method for Collecting Buccal DNA from Free-Ranging Non-Human PrimatesbioRxiv - Genetics 2021Quote: ... 750 µL of each supernatant was processed using a commercially available DNA clean-up system (Wizard SV Gel and PCR Clean-Up System; Promega, Madison, WI, USA), and the DNA was finally eluted with 50 µL pure water ...
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bioRxiv - Molecular Biology 2022Quote: For high-resolution DNA electrophoresis resolving the nucleosome repeats genomic DNA was purified from chromatin samples using the Wizard SV Gel and PCR Clean-Up System (Promega, catalog no. A9281) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunoprecipitated protein–DNA complexes and 5% input were analyzed by qRT-PCR using GO taq QPCR master mix (Promega, A6002, lot no. 0000385100) in triplicate using primers specific for PFKFB3 HREs ...
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bioRxiv - Biophysics 2022Quote: pHalo-H2B was generated by PCR amplification of the H2B coding region from an H2B-GFP template and cloned into a pFC14A backbone (Promega, Madison, WI, USA) to fuse the HaloTag to the C-terminus of H2B (56).
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bioRxiv - Microbiology 2022Quote: ... then 1 µL was transferred to a PCR tube containing 500 nM of each forward and reverse primer and 1X GoTaq mix (M7123, Promega, Madison, WI, USA) in 20 µL total volume ...
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bioRxiv - Developmental Biology 2024Quote: ... was amplified by PCR using tnnc1a-CDS primers (Table 1) and cloned into the pGEM®-T Easy Vector Systems (Promega, catalog #: A1360). A tnnc1a cDNA clone was confirmed by sequencing and used to make digoxigenin-labeled riboprobes with the Ambion™ MAXIscript™ SP6/T7 In Vitro Transcription Kit (Thermofisher ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by 85℃ heat inactivation for 1 hour and PCR-based genotyping (GoTaq Green Master Mix, Promega, and C1000 Touch Cycler, Bio-rad). Tcf12 [34] ...
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bioRxiv - Immunology 2024Quote: ... 5-GGATCCACACGGTGCAAAGAGAGACCC-3’ and 5′-TCGGCCTTTCAGACTAATCTTATCAGC-3’ The PCR products were gel purified and cloned into the pGEM-T vector (Promega; Madison, WI, USA). The inserted PCR fragments of individual clones were sequenced by Tsing KE Biological Technology ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The amplicons generated from the 5’ and 3’ race PCRs were subjected to purification and subsequent cloning into the pGEM-T easy vector (Promega Corp., WI, USA). The constructed vectors were sequenced (Plasmidsaurus ...
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bioRxiv - Microbiology 2023Quote: DNA from 1 ml of harvested phage stock was extracted using Wizard PCR DNA Purification Resin and Minicolumns (both Promega, Madison, WI, USA) as described previously [49] ...
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bioRxiv - Microbiology 2023Quote: ... were not screened for Wolbachia.All PCRs were performed in a final reaction volume of 20 μl including 4 μl of 5X reaction buffer (Promega, Madison, WI, USA), 1.5 μl of dNTPs (2.5 mM) ...