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Citations for Promega :
2701 - 2750 of 2907 citations for TREM 1 Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... We used 1 ml of cultures and isolated the genomic DNA using the Wizard Genomic DNA Purification Kit (Promega; Madison, WI). We used the DNA with the Illumina DNA Prep kit (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... DNA was isolated from 1 ml concentrated supernatant using Wizard PCR DNA Purification Resin and Minicolumns (both Promega, Madison, WI, USA) as previously described [74] ...
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bioRxiv - Genomics 2023Quote: ... The reaction was stopped with the addition of 150 μL IP Elution Buffer (1% SDS, 0.1 M NaHCO3) and 2 μL Proteinase K (Promega, Cat # MC5005), then incubated at 65 °C overnight to reverse crosslinks ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were washed thoroughly in PBS-T before incubation with secondary antibodies (anti-rabbit horseradish peroxidase, Promega WB401B 1:2,500 dilution) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... 20 ng of cDNA of each sample was used for the qPCR reaction (duplicate/sample) with ENG or α-tubulin specific primers (Supp. Table 1) and the GoTaq qPCR Master mix (Promega), in presence of CXR reference dye ...
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bioRxiv - Molecular Biology 2023Quote: ... at an enzyme-to-substrate ratio of 1:100 and subsequently digested overnight at 37°C with sequencing grade trypsin (Trypsin Gold, Mass Spec Grade, Promega, V5280) at an enzyme-to-substrate ratio of 1:50 ...
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bioRxiv - Immunology 2023Quote: ... and then diluted 2-fold with 200 mM ammonium bicarbonate for trypsin digestion (1:10 w:w, 37°C, 8h, Promega cat # V5113). After digestion ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA (1 μg) was reverse-transcribed using oligo(dT)18 and M-MLV reverse transcriptase II (Promega, Fitchburg, WI, USA).
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bioRxiv - Microbiology 2023Quote: ... Nanoluciferase bioluminescence was measured from the Hyp1-Nluc parasites by lysing the parasite culture 1:5 with 1x Nano-Glo Lysis Buffer (Promega, USA), and the addition of 1:1000 Nano-Glo (Promega) ...
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bioRxiv - Cell Biology 2023Quote: ... samples were resuspended in 50 µl of 50 mM ABC buffer and digested at 37°C for 16 h using 1 µg of trypsin (Trypsin Gold, Promega, V5280) and 1 µg of LysC (Wako Pure Chemical Corporation ...
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bioRxiv - Genomics 2023Quote: ... The reaction was stopped with the addition of 150 µL IP Elution Buffer (1% SDS, 0.1 M NaHCO3) and 2 µL Proteinase K (Promega, Cat # MC5005), then incubated at 65 °C overnight to reverse crosslinks ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were alkylated using 15 mM chloroacetamide at 37° C for 30min and further digested using sequencing-grade modified trypsin (1/50 w/w, ratio trypsin/protein; Promega, USA) at 37°C for 12h ...
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bioRxiv - Biochemistry 2023Quote: ... nLuc luciferase signal was measured by mixing 25 μL of Nano-Glo Luciferase Assay System (prepared 1:50 as recommended; Promega # N1120) with 25 μL of diluted reactions (diluted 1:5 in Glo Lysis Buffer ...
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bioRxiv - Biochemistry 2023Quote: ... 25 μL diluted lysate was mixed with an equal volume of Renilla-Glo Luciferase Assay System (prepared 1:100 as recommended; Promega # E2710) for 10 min at room temperature.
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bioRxiv - Cell Biology 2023Quote: ... 30 min) and diluted to 1M Urea with 200 mM ABC for trypsin digestion (1 μg, 37°C, overnight shaking, Promega, #V5113). On the next day ...
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bioRxiv - Molecular Biology 2023Quote: ... transfected cells grown for 48 hours in 96-well plates were washed with Hanks′ Balanced Salt solution (HBSS) and incubated with 1/1,000 dilution of furimazine stock solution (Promega, WI, USA). After incubation for 2 minutes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Membranes were washed in TBST and probed with anti-mouse HRP secondary antibody (Promega; 1:2000 in 5% BSA in TBST) for 1 h at room temperature followed by development in ECL (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were washed in TBST and probed with anti-mouse HRP secondary antibody (Promega; 1:5000 in 2% BSA in TBST) for 1 h at room temperature followed by development in ECL (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... The semisoft pellet of worms was weighed to ensure an equal number of worms in each sample (100 µg of worm pellet per sample) and mixed with 800 µL of 1× passive lysis buffer (Promega, #E1910). This was followed by eight cycles of sonication with 3 s on and 30 s off at an amplitude of 40% ...
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bioRxiv - Immunology 2023Quote: ... for 90 min at 37°C (enzyme-to-substrate ratio of 1:100) and subsequently digested overnight at 37°C with trypsin (Trypsin Gold, Mass Spec Grade, Promega, V5280) at an enzyme-to-substrate ratio of 1:50 ...
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bioRxiv - Biophysics 2023Quote: ... from the access handles by running this on a 1% agarose gel and gel purify the right DNA fragment with a gel purification kit (Promega, A9282).
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bioRxiv - Biochemistry 2023Quote: ... The reaction was quenched with DTT and urea was diluted to <1 M with 50 mM NH4HCO3 before adding MS-grade trypsin (Promega, V5113) in a ratio of 1:25 (trypsin:protein ...
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bioRxiv - Microbiology 2023Quote: The viral RNA derived from the lung and nasal turbinate samples was quantified using a protocol for quantifying the SARS-CoV-2 sub-genomic E gene RNA (sgE)29 using the GoTaq® Probe 1-Step RT-qPCR System (Promega).
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bioRxiv - Cancer Biology 2022Quote: ... to 10.5mL of 2.0 x 10^5 of Hek293 Cells (ATCC CRL1573) in growth media (DMEM + 10% FBS) was added 525uL of a 9:1 ratio of carrier DNA (Promega E4881) to a CLK nLuc construct-CLK1 CLK2 or CLK4 (Promega – NV1131 ...
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bioRxiv - Cancer Biology 2023Quote: ... Reactions were incubated for 1 hr at 30 °C and luciferase signal was measured using Dual-Glo Luciferase Assay System (Promega E2920).
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bioRxiv - Neuroscience 2023Quote: ... Homogenates were diluted 1/100 with the extraction buffer and mixed with the luminescent solution (CellTiter-Glo Luminescent Cell Viability Assay (Promega, RRID:SCR_006724)) ...
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were washed 3 × 10 min in TBS-T (0.1%) before 1hr incubation in secondary antibodies (anti-rabbit HRP or AP conjugate (Promega; 1: 5.000)) ...
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bioRxiv - Cell Biology 2023Quote: HeLa FlpIn were co-transfected with pCDNA5 constructs with gene of interest and construct of pOG44 recombinase in a 1:9 ratio with Fugene HD (Promega, E2311) under the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then diluted 50% with PBS before addition of sequencing grade trypsin (1 μg per sample, Promega Corp, V5111) and incubated overnight at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... The culture was diluted 1:100 in fresh LB supplemented with chloramphenicol and kanamycin including a 1:5000 dilution of the Nano-Glo® Luciferase Assay Substrate (Promega). Preliminary growth curves in the presence of the substrate showed no impact on growth at this concentration ...
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bioRxiv - Cell Biology 2024Quote: ... 5 µL of the schizont culture was aliquoted into white 96 well luminometer plates to which 45 µL of 1x NanoGlo Lysis buffer with 1:1000 NanoGlo substrate (Promega, USA) was added ...
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bioRxiv - Cancer Biology 2024Quote: ... The reaction mixture was then transformed at a ratio of 1:10 (reaction volume/volume competent cells) into HB101 competent cells (Promega #L2011). Spectinomycin-resistant colonies were screened by restriction digestion of miniprep DNA and subsequently validated by gel electrophoresis in a 0.8% agarose gel and by DNA sequencing ...
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bioRxiv - Systems Biology 2024Quote: ... protein digestion was performed overnight by addition of 50 nL of 0.01 ng nL-1 Lys-C (MS grade, Promega, Madison, USA) and 50 nL of 0.04 ng nL-1 trypsin (Promega ...
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bioRxiv - Genomics 2023Quote: ... cells were washed once with cold 1× PBS and the luciferase activities were measured with Perkin Elmer Envision using Promega Dual Luciferase Assay kit (Promega, E1960). The firefly luciferase activity was normalized to renilla luciferase activity for each well ...
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bioRxiv - Biochemistry 2023Quote: ... samples were resuspended in 6 M guanidine hydrochloride.250 µg of protein were digested overnight at 37 °C using trypsin with a 1:20 trypsin to sample ratio (Promega, #V5117). The next day ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then diluted 50% with PBS before addition of sequencing grade trypsin (1 μg per sample, Promega Corp, V5111) and incubated overnight at 37 °C ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were lyophilized and dissolved in digestion buffer (50 mM ammonium bicarbonate [ABC], 1 mM calcium chloride) and digested overnight using trypsin (Promega, #V5117) at trypsin ...
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bioRxiv - Biochemistry 2023Quote: ... cells were washed with 1 mL of 1X PBS and incubated in 100 μL of 1X Passive Lysis Buffer (Promega E1941) at room temperature for at least 30 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... and loaded on a micro S-trap column (Protifi, NY) and digested according to vendor protocol for 1 hour at 50 C by Trypsin Platinum (Promega, WV). Digested material was then eluted from the column by centrifuge at 1000 rpm for 2 minutes and dried in speedvac (Eppendorf ...
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bioRxiv - Systems Biology 2024Quote: ... and an aliquot of approximately 25 µg of protein was digested with Trypsin (Promega, Trypsin to protein ratio of 1:50) at 37°C overnight ...
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bioRxiv - Physiology 2024Quote: ... Protein (50 μg per sample) was digested overnight at 37°C with LysC (Wako Chemicals) at 1:50 (w/w) ratio and trypsin (Promega, V5113) at 1:100 (w/w ...
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bioRxiv - Biochemistry 2024Quote: ... 20 µg of protein was diluted 20 times with 50 mM ABC (no guanidine) and trypsin was added with a 1:20 trypsin to sample ratio (Promega, V5117). Protein were digested overnight at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were washed 3 times in TBST for 15’ per wash and incubated 1 hour in HRP-conjugated anti-rabbit (Promega, W4011) or anti-mouse (Promega ...
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bioRxiv - Neuroscience 2024Quote: ... The homogenate was centrifuged at 16,000 x g for 10 min and the supernatant was collected and diluted to 1:200 with deiodinized water for ATP measurement using the Cell Titer-Glo luminescent cell viability assay kit (Promega, G7571) and 1:10 for determining the protein concentration ...
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bioRxiv - Biochemistry 2024Quote: ... The final reduced and alkylated flow through from the column was mixed with 1 mg/mL solution of trypsin enzyme (Promega, V511A) in ratio 1:10 (w/w ...
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bioRxiv - Biochemistry 2024Quote: ... 1 µg of protein was diluted 5 times with 50 mM ABC and trypsin was added with a 1:20 trypsin to sample ratio (Promega, V5117). Protein were digested 24 h at 37 °C ...
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bioRxiv - Biochemistry 2024Quote: ... 20 µg of protein was diluted 10 times with 50 mM ABC (no Guanidine) and trypsin was added with a 1:20 trypsin to sample ratio (Promega, V5117). Protein were digested overnight at 37 °C ...
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bioRxiv - Immunology 2024Quote: HEK293T cells (∼6 × 105 cells per 6-well) were transfected with 1-3 μg of the indicated constructs using FuGENE® HD Transfection Reagent (#E2311, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... 20 µg of protein was diluted 20 times with 50 mM ABC (no guanidine) and trypsin was added with a 1:20 trypsin to sample ratio (Promega, V5117). Protein were digested overnight at 37 °C ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was then incubated at 37°C for overnight trypsin digestion (trypsin 1:50 protein w/w ratio; Promega, Madison, WI). The digestion reaction was quenched by adding formic acid (FA ...