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Citations for Promega :
2651 - 2700 of 3088 citations for 7 Bromo 6 chloro 3 3 3 hydroxy 2 piperidyl 2 oxopropyl quinazolin 4 3H one monohydrobromide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... by using 4 μL of FuGENE HD transfection reagent (Promega #E2311) according manufactured protocol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Then 4 μL/well of Bright-Glo Luciferase detection reagent (Promega) was added to assay plates and incubated for 5 min at room temperature ...
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bioRxiv - Genomics 2019Quote: RNA samples (4 ug) were mixed with random hexamers (Promega, #C118A) and heated at 70 °C for 5 minutes ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell death was measured after 4 days using CytoTox Glo (Promega).
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bioRxiv - Microbiology 2019Quote: Cells were harvested in 4 M guanidine thiocyanate (Promega, United States) in a 1% sodium lauryl sarcosine (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... Resin was then washed 4 times with Resin Wash Buffer (Promega). Complexed proteins were eluted in SDS Elution Buffer (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... and overnight ligation at 4°C (T4 DNA ligase, Promega, USA). Ligated chimeras were transformed into DH5α (ThermoScientific ...
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bioRxiv - Developmental Biology 2023Quote: ... 100 nM dT-primer and 4 U RNase Inhibitor (Promega, N2611). Reverse transcription was performed at 42°C for 90 min after filling up to 10 μl with RT buffer mix for a final concentration of 1x superscript II buffer (Invitrogen) ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were transfected with 750 ng of the pMXs-IP vectors with 2.3 μl FuGENE 6 (Promega, E2691). The culture medium was replaced with fresh DMEM with 10% FBS and C2C12 cells were seeded at a concentration of 1×105 cells/well in a 12-well plate on day 3 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with a control Flag or HA-GSK-3βWT human plasmid using Fugene 6 (Promega #E2693) in a 2:1 ratio and incubated for 3 hours in a CO2 incubator ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were then transfected with 1500 ng pcDNA-FLAG-HA-sscGAS plasmid complexed with FuGene 6 reagent (Promega) according to manufacturer’s instructions or treated with FuGene 6 alone as a negative control ...
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bioRxiv - Neuroscience 2020Quote: ... pCAG-DCC:TDTOMATO and pCAG-DCCkanga:TDTOMATO plasmids (1 µg) were transfected into the plated U251 cells using FuGENE 6 (Promega) in Opti-MEM (Gibco ...
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bioRxiv - Immunology 2020Quote: ... were transfected with 4.5 µg of the pBabe-puro-NCAM140 mApple construct (NCBI reference sequence: NM_000615.6, transcript variant 1) using Fugene 6 Transfection Reagent (Promega). Supernatant containing viral particles was collected and concentrated using PEG-IT (System Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... Generation of infectious lentiviral particles and subsequent cell transduction was performed as previously described.26 FuGENE 6 (Promega) was used to transfect select plasmids ...
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bioRxiv - Microbiology 2021Quote: ... 293T cells were co-transfected with 1.5 mg of spike expression plasmids in pCDNA3 and 0.5 mg pmCherry-N1 using Fugene 6 and following the manufacturer’s instructions (Promega). Vero cells were treated with CellTracker™ Green CMFDA (5-chloromethylfluorescein diacetate ...
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bioRxiv - Molecular Biology 2022Quote: ... the proteins were digested overnight at 37 °C with 6 ng μL-1 of Asp-N (Promega, UK). Peptides were extracted in 2 % v/v formic acid ...
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bioRxiv - Microbiology 2019Quote: ... were reverse transfected with 50 ng pCAGEN-HA plasmid and 12.5 ng pcDNA3.1+/C-(K)DYK protease-plasmid using Fugene 6 (Promega). After 24 h ...
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bioRxiv - Cell Biology 2021Quote: ... zyxin(-/-) MEFs were transfected with 5 μg total DNA using the Fugene® 6 transfection reagent (E2691, Promega) and plated on glass coverslips.
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bioRxiv - Cell Biology 2019Quote: ... or Atto 550 (MolBi Tech)) were delivered into the nucleus via transfection using the FuGENE 6 reagent (Promega), while EdU (Life Technologies) ...
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bioRxiv - Biochemistry 2019Quote: ... 0.2 μg pLEXm plasmid encoding WT or mutant KAP1 and 0.4 ng plasmid encoding Renilla luciferase using 1.5 μl of FuGENE 6 (Promega). Luciferase activity was measured 48 h post-transfection in cell lysates with the Dual Luciferase assay kit (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... 32μg SIV4+ (49)and with or without 1μg of pcDNA3.1 Vpx expression plasmid using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... were co-transfected with 0.5 μg of spike expression plasmids in pCDNA3 using Fugene 6 and following the manufacturer’s instructions (Promega). Cell-cell fusion was measured using an Incucyte and determined as the proportion of green area to total phase area ...
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bioRxiv - Cell Biology 2020Quote: ... and the solution was added to OMEM (75 μL) containing 6 μL Fugene HD reagent (Promega, Madison, WI). The mixture was incubated at room temperature for 10 minutes and gently added to the growth medium on the cultured cells ...
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bioRxiv - Microbiology 2021Quote: ... Plates were incubated for 24 h and viability was measured following a 6 h incubation with CellTiterBlue (Promega). Viability was quantified by fluorescent measurement (Ex ...
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bioRxiv - Developmental Biology 2022Quote: ... were transfected into NCCIT cells (ATCC) at ∼70% confluency using a 6:1 ratio of Fugene HD (Promega) for 48 hours in ATCC-formulated RPMI media ...
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bioRxiv - Cancer Biology 2022Quote: ... 1mg of the plasmid was transfected to 293T cells (ATCC #CRL-11268) using FuGENE 6 Transfection Reagent (Promega) to produce the retroviruses ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected with 8 µg of pcDNA3.1-GFP-mNLS-K145Q using Fugene 6 Transfection reagent (Promega, E2692) per manufacturer protocol ...
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bioRxiv - Microbiology 2024Quote: ... Cells were transfected with 500 ng of each plasmid using 0.4 µl of Fugene 6 according to the manufacturer’s instructions (Promega) for 40 hours ...
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bioRxiv - Microbiology 2023Quote: Lentiviral particles were produced by transfection of HEK293T cells in T150 flasks using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... DF1 cells were transfected with the different RCAS retroviral plasmids using FuGENE 6 Transfection reagent (Promega, Cat. E2691), accordingly to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested off the beads by adding 6 μL of 1 μg/μL sequencing grade Trypsin (Promega), diluted in 50 mM acetic acid ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with plasmid DNA by the calcium phosphate or lipofection method using FuGene 6 (Promega, WI) (Yoshida et al. ...
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bioRxiv - Neuroscience 2023Quote: HEK293T cells were transfected with either Tau-encoding or control constructs using Fugene 6 (Promega, cat. no. E2691) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was assessed 6 days post-treatment using a Cell-TiterGlo 3D Cell Viability assay (Promega, G9683), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were transfected with LDLR-GFP (WT, C75W, and C75W/C34W) for 48 h with Fugene 6 (Promega). Cells were starved in 5% LPDS media (Millipore Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... precipitated proteins were resuspended in 0.1% RapiGest with 6 μL of sequencing-grade trypsin (Promega, 0.5 μg/μL) added to each sample and digested overnight at 37°C ...
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bioRxiv - Plant Biology 2024Quote: ... methylase was amplified using M.SssI_HindIII forward and reverse primers (Sup Table 6) cloned into pGEMT-Easy (Promega, USA) and sequenced for verification ...
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bioRxiv - Genetics 2024Quote: ... Effects on endogenous FAS exon 6 inclusion were determined by PCR using GoTaq flexi DNA polymerase (M7806, Promega) and the following primers:
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bioRxiv - Molecular Biology 2024Quote: Cells were seeded in 12-well plates at a density of 200,000 per well and transfected using 1.5 µl/well FuGENE 6 (Promega) with 5 ng of CMV-Renilla plasmid (pTG888) ...
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bioRxiv - Cell Biology 2022Quote: Proliferation assays were performed in 96 well plates (2 × 103 NMCCs/well) and assayed at 48 h using the CellTiter96® AQueous One Solution Cell Proliferation Assay kit (MTS; Promega, Madison, WI) (38) ...
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bioRxiv - Immunology 2022Quote: ... Pseudovirus titers were measured by infecting 293T/TRCAT cells for 72 hours before measuring luciferase activity (ONE-Glo™ Luciferase Assay System, Promega, Madison, WI). Pseudovirus titers were expressed as relative luminescence units per milliliter of pseudovirus supernatants (RLU/ml) ...
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bioRxiv - Cancer Biology 2020Quote: MTS growth assays were performed on adherent cells by plating 1000-5000 cells per well in a 96-well plate and assaying using CellTiter 96® AQueous One Solution (Promega, Madison, WI) and incubating for 2 hours and reading at 490nm ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: The evaluation of the cytotoxicity of NBCoV small molecules in the different cell types was performed in parallel with the antiviral activity assay and measured by using the colorimetric CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) (Promega, Madison, WI) following the manufacturer’s instructions.
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bioRxiv - Plant Biology 2020Quote: ... and five early developmental buds (one early developmental sample) per individual using the Maxwell 16 LEV Plant RNA Extraction Kit (Promega Corporation, Madison, WI).
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bioRxiv - Cancer Biology 2020Quote: Cells were seeded in 96-well plates and then maintained in the presence of vehicle or increasing doses of GSK591 for six days before the addition of 20 μL CellTiter 96 AQueous One Solution per well (Promega Corporation, Madison, WI). Plates were incubated for 2 hours before absorbance at OD490 was measured with a Synergy H4 Hybrid microplate reader (Bio Tek ...
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bioRxiv - Cancer Biology 2021Quote: One microgram of total RNAs from cells and tissues were reversely transcribed into cDNAs using M-MLV reverse transcriptase (Promega, Madison, WI, USA). Quantitative PCR analysis was performed by using SYBR Green SuperMix (Yeasen Biotech ...
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bioRxiv - Cell Biology 2022Quote: ... Cell viability at 72-h incubation was measured by MTS cell proliferation colorimetric assay (CellTiter 96® AQueous One Solution Cell Proliferation Assay system, Promega, G3582, Italy). Cell numbers at 0 ...
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Farnesyltransferase inhibition overcomes the adaptive resistance to osimertinib in EGFR-mutant NSCLCbioRxiv - Cancer Biology 2022Quote: Viability of untreated cells or treated cells for 5 days was assessed using CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) (Promega®, ref:G3580) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2019Quote: ... a proliferation rate was calculated using the data obtained from MTT colorimetric assays (CellTiter 96® AQueous One Solution Cell Proliferation Assay; Promega; Madison, WI). Data from five replicates for at each time point were analyzed using Graphpad Prism software with a p value less than 0.05 considered significant.
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bioRxiv - Molecular Biology 2024Quote: Metabolic activity of CpdG or DMSO treated and oexORP7 or oexMYC cells was measured using CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega cat #: G358C), according to the manufacturer’s instructions ...