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Citations for Promega :
2551 - 2600 of 3357 citations for Propane 1 2 Diyl Diacetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Cells were then incubated with CellTiter 96 Aqueous One Solution Proliferation Solution for 1 hour (Promega, Fitchburg, WI). Absorbance of the solution was then read at 490 nm ...
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bioRxiv - Biochemistry 2020Quote: ... The eluent was equally divided into three for digestion with either: 10:1 (w/w) Trypsin Gold (Promega), 7.5:1 (w/w ...
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bioRxiv - Immunology 2020Quote: ... the membrane was incubated with HRP-conjugated goat anti-rabbit IgG diluted 1:2000 (Promega, Madison, Wisconsin, USA) for 2 h at RT ...
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bioRxiv - Immunology 2021Quote: ... qRT-PCR was performed in 12 μL reactions using GoTaq Probe 1-step RT-qPCR System (Promega, US) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ATP release from THP-1 cells was determined by the ENLIGHTEN ATP Assay System Bioluminescence Detection Kit (Promega). Cells were grown in 6-well plates and starved overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... and sequentially digested with Lys-C (Wako) (protein:enzyme ratio 1:50, o/n at RT) and trypsin (Promega) (protein:enzyme ratio 1:50 ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 μg of DNA-free RNA was then used for reverse transcription using M-MLV Reverse Transcriptase (Promega) in the presence of RiboLock ...
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bioRxiv - Cell Biology 2021Quote: ... was then used to transcribe 1 µg of total RNA to cDNA using Oligo(dT)15 primers (Promega). KAPA Probe Fast qPCR Master Mix (2X ...
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bioRxiv - Molecular Biology 2021Quote: 1 uL of pUG cDNA was then input into a 20 uL GoTaq PCR reaction (Promega; Cat # M7123) with the first adapter specific primer (Shukla et al. ...
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bioRxiv - Immunology 2020Quote: ... the indicated NLRP1 construct (0.02 μg) and RFP (to 1 µg) with FuGENE HD according to manufacturer’s instructions (Promega). At 24 h cells were treated with DMSO or VbP (10 µM) ...
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bioRxiv - Cancer Biology 2020Quote: pET20b-6xHis-ARF5D17 or pGEX-5x-1-hGGA3 [VHS-GAT] were transformed into BL21 (DE3) pLysS bacteria (Promega). Single colonies were grown in lysogeny broth (LB ...
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bioRxiv - Cell Biology 2020Quote: ... Isolated RNA (1 μg) was used to synthesize cDNAs using Murine Moloney Leukemia Virus reverse transcriptase (MMLV, Promega). Subsequently ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were transfected with the Piggybac plasmid plus transposase at a 3:1 ratio using Fugene HD (Promega) and selected with G418 (300 µg/mL ...
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bioRxiv - Developmental Biology 2022Quote: ... were transfected into NCCIT cells (ATCC) at ∼70% confluency using a 6:1 ratio of Fugene HD (Promega) for 48 hours in ATCC-formulated RPMI media ...
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bioRxiv - Biochemistry 2022Quote: ... Elution was conducted in 3 beads volume of proteasome buffer containing 1 μL of TEV protease (Promega, PRV6101) for 1 hr at 37°C.
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bioRxiv - Neuroscience 2022Quote: ... Protein bands were detected with horseradish peroxidase (HRP)-conjugated rabbit or mouse secondary antibody [1:1,000 dilution] (Promega). The chemiluminescence signal was imaged using an Odyssey XF Imager (LI-COR ...
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bioRxiv - Neuroscience 2023Quote: ... Digestion reaction for proteins was initially set up with 1 μg of MS grade LysC (Promega, Cat# PI90307) for 3 hours at RT and then continued overnight with addition of 2 μg of trypsin (Promega ...
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bioRxiv - Plant Biology 2024Quote: ... and incubated for 1 h at room temperature before measuring luminescence using a GloMax Explorer plate reader (Promega). A standard curve was used for quantification of the UDP produced ...
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bioRxiv - Systems Biology 2024Quote: ... followed by a 3:1 dilution with 100mM ammonium bicarbonate and addition of 2µg sequence-grade trypsin (Promega). Samples were digested at room temperature overnight and acidified with formic acid (final concentration 1%) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 μg of total RNA was used for first strand cDNA synthesis with M-MLV reverse transcriptase (Promega) and oligo (dT ...
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bioRxiv - Cell Biology 2024Quote: 20×106 cells were collected in cold PBS by scraping and lysed in RIP buffer (251mM Tris-HCl pH 7.4, 1501mM KCl, 5mM EDTA, 0.5% Nonidet P-40, 201U1ml−1 RNAsin (Promega), 0,51mM dithiothreitol (DTT ...
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bioRxiv - Systems Biology 2024Quote: ... RNA was refolded by adding 88.8 μL 300 mM sodium cacodylate and 1 μL RNasin Plus (Promega N2611) and 10 min incubation at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... Relative quantification of viral RNA was performed using the GoTaq® 1-Step RT-qPCR System kit (Promega). The mixture contained 5 μL of 2x Master Mix ...
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bioRxiv - Molecular Biology 2024Quote: ... The samples were diluted with 17 µL of 50 mM HEPES (pH 8.5) and digested with 1 µL of 0.2 mg/mL trypsin (Promega) in 0.2 mM HCl at 37°C with interval mixing at 2000 RPM for 30 sec every 15 min.
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bioRxiv - Neuroscience 2024Quote: ... Specific amplicons were prepared using several primer pairs (Table 1) in combination with GoTaq© DNA Polymerase (Promega).
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bioRxiv - Cancer Biology 2024Quote: ... then washed with 1 ml of PBS and lysed using 60 μl of Cell Culture Lysis Reagent (Promega). 20 μl of cell lysate was added to 20 μl of Luciferase Substrate (Promega) ...
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bioRxiv - Microbiology 2024Quote: ... 50 to 100 ng of template were used per reaction with 1.5 µl rRNasin 40 U.ml-1 (Promega), 5 µl 5x optimized transcription buffer (Promega) ...
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bioRxiv - Cell Biology 2024Quote: ... Tryptic digestion was performed by incubating the beads with 1 µg MS-grade TPCK trypsin (Promega, Madison, WI) dissolved in 200 μL of 50 mM ammonium bicarbonate (pH 8.3 ...
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bioRxiv - Genetics 2024Quote: ... 1 μL cDNA was used in 10-μL reactions containing Brilliant III SYBR Green qPCR Master Mix (Promega). Biological replicates were analyzed as duplicates ...
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bioRxiv - Cell Biology 2024Quote: ... HaloTag-receptor expressing mESCs were fluorescently labeled with either 1 µM of HaloTag AlexaFluor 488 ligand (Promega, G1001) or 3.5µM of HaloTag AlexaFluor 660 ligand (Promega ...
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bioRxiv - Biochemistry 2024Quote: The amount of intracellular Glu was measured in 1×106 cells using the kit Glutamate-Glo (J7021; Promega) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... then reverse transcribed for 1 hr at 37 °C using M-MLV reverse transcriptase (Promega, Madison, WI; M1705) in the presence of RNase inhibitor (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: Real-time PCR was carried out using the Promega GoTaq® Probe 1-Step RT-qPCR System (Promega) with Sarbecovirus-specific envelope gene primers from bat RNA samples as published by (Corman ...
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bioRxiv - Biochemistry 2024Quote: ... were digested overnight at 37 °C using trypsin with a 1:20 trypsin to sample ratio (Promega, #V5117). The next day ...
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bioRxiv - Microbiology 2023Quote: ... the supernatants were aspirated from the wells and 50μl of a 1:8000 concentration of ViviRen (Promega, #E6491) in viral media were added to each well ...
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bioRxiv - Biophysics 2023Quote: The activity of the masked antagonists was analyzed using a PD-1/PD-L1 Blockade Bioassay (Promega, J1250) according to a modified manufacturer’s protocol as described previously(32) ...
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bioRxiv - Cell Biology 2023Quote: ... the gel plugs were digested by using 1:50 (Trypsin: Protein amount ratio) Sequencing Grade Modified Trypsin (Promega) at 37 °C overnight ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested off the beads by adding 6 μL of 1 μg/μL sequencing grade Trypsin (Promega), diluted in 50 mM acetic acid ...
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bioRxiv - Immunology 2022Quote: ... Cell pellets were solubilized with 10 μL of 8M urea and 10 μL of 1% Protease MAX (Promega) in 100 mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: Real-time PCR was carried out using the Promega GoTaq® Probe 1-Step RT-qPCR System (Promega) with Sarbecovirus-specific envelope gene primers from bat RNA samples as published by (Corman ...
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bioRxiv - Microbiology 2023Quote: ... and 1:10000) were established using the genes previously cloned in pGEM-T Easy Vector (Promega, Madison, USA). The data were determined in terms of copies of the nisA and nisRK genes / μL of qPCR reaction ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were directly digested by incubation with sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin) overnight at 37°C ...
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bioRxiv - Systems Biology 2023Quote: ... Samples were then digested by a 1:50 (trypsin to protein) ratio of sequencing grade modified trypsin (Promega) for 16 hours at 600 rpm and 25°C ...
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bioRxiv - Microbiology 2023Quote: ... The pellet was resuspended in 0.1M TEAB and overnight (∼16 h) digestion with trypsin/Lys-C mix (1:25 protease:protein; Promega) at 37° C was performed with constant mixing using a thermomixer ...
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bioRxiv - Microbiology 2023Quote: ... Trypsin digest was allowed overnight at 37°C by the addition of 1 μg of trypsin (#V5111, Promega) to each sample ...
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bioRxiv - Genomics 2023Quote: ... 1 μL of each primer (10 μM) and 12.5 μL of GoTaq Green Master mix (Promega®, USA). The PCR was run in a thermocycler (BioRad ...
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bioRxiv - Microbiology 2023Quote: ... dissolved in 2 volumes of 0.1 M Tris-HCl (pH 8.0) with the addition of 1 μg of trypsin (Sequencing Grade Modified, Promega) and incubated for 12 hours at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... After that pancreatic enzyme was added at a ratio of 1:50 (Trypsin Gold, Promega, Fitchburg, WI, USA), and incubated overnight at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... Total proteome peptide pools were generated by sample digestion overnight with 1:100 sequencing grade trypsin (V5113; Promega). Generated peptide pools were quantified by Nanodrop ...
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bioRxiv - Plant Biology 2023Quote: ... 1 μg protein was used for dual luciferase transactivation assay using Dual-Luciferase® Reporter Assay System (Promega) following manufacturer’s protocol ...