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Citations for Promega :
2551 - 2600 of 2952 citations for Mono 2 Ethyl 5 Hydroxyhexyl Phthalate 13C4 99% Dehp Metabolite Ix 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... the eluted proteins were digested with 10 μg/mL modified trypsin (Sequencing grade, Promega, USA) at 37 °C for 16 h ...
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bioRxiv - Biochemistry 2020Quote: ... at a cell density of 1×106 cells ml-1 using FuGENE HD reagent (Promega), grown in Sf900 II SFM media (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2020Quote: ... Each well was added with 20 mL of CellTiter 96R AQueous One Solution Reagent (Promega) and absorbance was measured in a microplate reader at 490 nm ...
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bioRxiv - Cell Biology 2022Quote: ... proteins in the plugs where digested overnight with Trypsin (10μg/ml) (Promega, Madison, WI, USA) at 37°C in a 25-mM NH4HCO3 buffer (0.2 µg trypsin in 20 µL) ...
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bioRxiv - Biochemistry 2021Quote: ... 1x protease inhibitor mix (Benzamidine & PMSF)) supplemented with 7 U/ml DNase I (M610A, Promega) and lysed by Dounce homogenization and incubation on ice for 30 min ...
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bioRxiv - Microbiology 2022Quote: ... Reactions were stopped by adding a buffer containing 50 μg/mL of proteinase K (Promega) in a buffer of 3 mM CaCl2 ...
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bioRxiv - Neuroscience 2019Quote: ... Proteins captured on magnetic beads were digested with 1.5 mg/mL trypsin (Promega Cat# V5111) in 50 mM ammonium bicarbonate overnight at 37 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... 2.5ng ml-1 recombinant human fibroblast growth factor (rhFGF) (Promega, Cat#G5071, Madison, WI, USA), and 1% chicken embryonic extract (Seralab ...
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bioRxiv - Immunology 2020Quote: ... Mice were tested individually in duplicates by stimulation with recombinant luciferase (13μg/ml, Promega, #E1701), SARS-CoV-2 spike protein (10μg/ml) ...
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bioRxiv - Biophysics 2022Quote: ... at a cell density of 1 × 106 cells ml−1 using FuGENE HD reagent (Promega), grown in Sf900 II SFM media (Thermo Fisher) ...
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bioRxiv - Biophysics 2022Quote: ... and incubated with a 20 µg/ml solution of the HaloTag amine (O4) ligand (Promega) overnight ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins captured on magnetic beads were digested with 1.5 mg/mL trypsin (Promega Cat# V5111) in 50 mM ammonium bicarbonate overnight at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... 200 ml of media was treated with 4 U of RQ1 RNase-free DNase (Promega) for 1 hour at 37°C ...
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bioRxiv - Immunology 2023Quote: ... mice were injected with 5µl/g body weight of 15mg/ml VivoGlo™ Luciferin (Promega) and imaged 10 minutes after injection in an IVIS machine (PerkinElmer ...
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HIVtat Alters Epithelial Differentiation State and Increases HPV16 Infectivity in Oral KeratinocytesbioRxiv - Microbiology 2023Quote: ... 200 ml of media was treated with 4 U of RQ1 RNase-free DNase (Promega) for 1 hour at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... The pellet was then suspended in 1.5 mL Fast-Break lysis buffer (Promega, Madison, WI) with 1 mM PMSF ...
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bioRxiv - Neuroscience 2023Quote: ... the luminescent signal was measured weekly by adding 1.5 mg/ml Beetle luciferin (E1601, Promega) for 48h to the neurospheroid cultures ...
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bioRxiv - Molecular Biology 2023Quote: ... A portion of the sample was further treated with 0.2 mg/ml RNase A (Promega) at 37°C for 10 min ...
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bioRxiv - Molecular Biology 2024Quote: ... and then placed in a 50 mL solution of 1 ×Diamond Nucleic Acid Dye (Promega) in 0.2 ×TBE buffer pH 7.6 to rock in the dark for 30 min ...
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bioRxiv - Physiology 2020Quote: ... The beads were resuspended in 10 μl of reverse transcriptase mix (100□U, SuperScript II, Invitrogen; 10 U, RNAsin, Promega, Madison, Wisconsin, USA), 1□×□Superscript II First-Strand Buffer ...
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bioRxiv - Genomics 2019Quote: ... To this was added: 1 µl of EcoRV-digested pBlueScript KS II+ (∼100 ng) that had been treated with calf intestinal phosphatase (Promega Corp., Madison, WI), and 0.1 µl of T4 DNA ligase (NEB) ...
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bioRxiv - Systems Biology 2020Quote: ... TFE was then diluted to 25% with 50mM ABC, LysC (Wako Chemicals, 1:100 lysC:protein ratio) and sequencing-grade modified trypsin (Promega, 1:50 trypsin:protein ratio) was added and incubated overnight at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... TFE was then diluted to 25% with 50 mM ABC, LysC (Wako Chemicals, 1:100 lysC:protein ratio) and sequencing-grade modified trypsin (Promega, 1:50 trypsin:protein ratio) was added and incubated overnight at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... Gel pieces were resuspended in 20 μL l of trypsin solution (25 ng/ μL trypsin in 100 mM NH4HCO3) (Promega, Cat. No V5111) and incubated at 4°C for 20 min ...
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bioRxiv - Bioengineering 2019Quote: ... beads were equilibrated in 100 mM ammonium bicarbonate with 3 washes prior to on-bead digestion with trypsin (800 ng; Promega cat. nr. V5280) overnight at 37°C 800 rpm ...
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bioRxiv - Pathology 2021Quote: ... Transient knockdowns were performed with mycoplasma free cells using 100 nM siRNAs comprising of three different probes (Gene Pharma, Shanghai, China) and FugeneHD (Promega, Madison, WI, USA) for 24 hours ...
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bioRxiv - Biochemistry 2021Quote: ... Tryptic samples were subsequently diluted to 2M urea in 100mM Tris/HCl pH 8 and digested over night at 37°C (Promega, enzyme:protein 1:100). Samples were adjusted to pH 2 using 10% trifluoroacetic acid (TFA ...
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bioRxiv - Immunology 2021Quote: ... The plates were washed three times and the reaction was developed with 100 µL ready-to-use TMB solution (Promega, Madison, WI, USA) at RT for 20 min in the dark ...
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bioRxiv - Molecular Biology 2023Quote: ... containing 300 ng of the recombinant T/F LTR-pGL3 plasmid and 100 ng of recombinant control insert DNA-pTarget plasmid (Promega Corporation, Madison, WI) for the Tat minus transfection or 100 ng of recombinant HIV-1C consensus or autologous Tat-pTarget plasmid (obtained from the same sample from which the LTR was derived ...
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bioRxiv - Molecular Biology 2022Quote: ... Then wells were washed again three times with PBS-T (100 μl per well) and incubated with a secondary mouse-HRP antibody (goat, Promega, Madison, WI, USA) (50 μl per well ...
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bioRxiv - Molecular Biology 2023Quote: ... or stimulated for 10 minutes or 1 hour by BRET through activation of NanoLuc’s bioluminescence with its substrate furimazine (1:100 dilution of Promega nano-Glo Live Assay). After treatment ...
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bioRxiv - Biochemistry 2023Quote: ... each sample was hydrolyzed to 100 µg of protein in a 50: 1 ratio using trypsin (trypsin Gold, MassSpec Grade, Promega, Fitchburg, WI, USA). After enzymolysis at 37 for 14 h ...
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bioRxiv - Molecular Biology 2024Quote: ... and digested with Lys-C (1/200 weight of protein; Fujifilm Wako) and sequencing grade modified trypsin (1/100 weight of protein; Promega, Madison, WI, USA) at 37 °C overnight in 0.1 % Rapigest ...
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bioRxiv - Cancer Biology 2022Quote: ... The organoids were incubated for 5 days at 37C and assayed using the CellTiter-Glo® 3D Cell Viability Assay (Promega Cat # G9681) and read for luminescence using the EnVision 2105 plate reader ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 µl of the RT product (diluted 1:5) was used for semi-quantitative PCR or qPCR reactions with Promega PCR Mix (Promega, Madison, Wisconsin, USA) and SYBR Green PCR Mix (Applied Biosystems ...
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bioRxiv - Genetics 2019Quote: Buccal cell samples were collected and prepared as for the standard processing method with the exception that 3 μl 5 x AmpSolution™ (Promega Corporation, USA), was added to the PCR reagents in place of low TE buffer ...
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bioRxiv - Genomics 2020Quote: ... Cell pellets were allowed to thaw for 5 minutes in ice and RNA was purified using the SV total RNA isolation system (Promega Corporation, Wisconsin, USA). An additional DNAse treatment was added by using the rigorous DNAse treatment with Turbo DNA-free (Ambion ...
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bioRxiv - Bioengineering 2021Quote: ... Barcodes were amplified using forward primer MWV 486 and a 5’-biotinylated reverse primer MWV 358 (Table S1) using standard PCR conditions with Gotaq G2 flexi DNA polymerase (Promega, Leiden, the Netherlands). The biotinylated PCR products were then hybridised to a pool of MagPlex-TAG microspheres (Table S3 ...
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bioRxiv - Microbiology 2020Quote: ... followed by a reverse transcription using a universal degenerated oligo specific to all 5′ non-coding sequences of BTV-1 segments (BTV/Uni1; 5′ GTTAAAWHDB 3′) and the GoScript Reverse Transcription (RT) System (Promega, Madison, WI, USA). The cDNAs obtained for the segment 7 were then quantified with a real time quantitative PCR (qPCR) ...
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bioRxiv - Microbiology 2020Quote: Faecal DNA was extracted and purified using a combination of repeated bead-beating (method 5) (Costea et al, 2017) and the Maxwell 16 Tissue LEV Total RNA Purification Kit (Promega, Maddison, WI, USA), with STAR (Stool transport and recovery ...
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Farnesyltransferase inhibition overcomes the adaptive resistance to osimertinib in EGFR-mutant NSCLCbioRxiv - Cancer Biology 2022Quote: Viability of untreated cells or treated cells for 5 days was assessed using CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) (Promega®, ref:G3580) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... relative luminescent units) was detected from the ROS signals after 5 min incubation using a GloMax® 96 Microplate Luminometer (Promega, Madison, WI).
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bioRxiv - Immunology 2020Quote: Activity of the inflammatory caspases 1/4/5 was measured using a commercially available Caspase-Glo® 1 Inflammasome Assay (Promega, WI, USA) from HFFs seeded in 96-well plates (2×104 cells per well) ...
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bioRxiv - Microbiology 2021Quote: ... knowlesi elongation factor-1 alpha (pkef1-alpha) 5’ UTR were isolated from agarose gels and cloned into the pGEM-T vector (Promega, Madison, WI, USA). To replace the pfcam 5’ UTR sequence that drives luciferase transcription in the pD-pfcam-Luc ...
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bioRxiv - Cell Biology 2019Quote: ... containing T7 promoter at the 5’ end at 37 °C using the T7 RiboMax Express Large-Scale RNA Production System (Promega Corp., Madison, WI), following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... CDC was measured after incubation for 3 hours at 37 °C 5% CO2 with a luminometer using the CytoTox-Glo Cytotoxicity Assay (Promega; Cat. Nr.: G9291) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... Immunoprecipitated protein–DNA complexes and 5% input were analyzed by qRT-PCR using GO taq QPCR master mix (Promega, A6002, lot no. 0000385100) in triplicate using primers specific for PFKFB3 HREs ...
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bioRxiv - Plant Biology 2022Quote: ... benthamiana leaf discs were ground to a fine powder in liquid nitrogen in 2 ml Eppendorf Safe-Lock tube (1 leaf disc in each tube) and subsequently incubated 5 min at room temperature (RT) with 200 µl of the Passive lysis buffer (Promega catalog number E1910). The resulting crude leaf extract was centrifuged at 20,000 g for 2 min at RT ...
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bioRxiv - Immunology 2024Quote: ... 5-GGATCCACACGGTGCAAAGAGAGACCC-3’ and 5′-TCGGCCTTTCAGACTAATCTTATCAGC-3’ The PCR products were gel purified and cloned into the pGEM-T vector (Promega; Madison, WI, USA). The inserted PCR fragments of individual clones were sequenced by Tsing KE Biological Technology ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were then solubilized in 50 mM ammonium bicarbonate for a reduction-alkylation step (dithiothreitol 5 mM – iodoacetamide 10 mM) and an overnight digestion with 300ng of sequencing-grade porcine trypsin (Promega, Fitchburg, MA, USA). Digested peptides were resuspended in 0.1% formic acid (solvent A ...