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Citations for Promega :
2551 - 2600 of 2721 citations for 6 N CARBETHOXY 3 CHLORO 7 8 DIHYDRO 5H PYRIDO 4 3 C PYRIDAZINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... On bead digestion was performed in 50 µl of ammonium bicarbonate solution containing 4 µl of Trypsin/LysC (Promega) solution and incubated at 37°C for 3 h in a thermomixer at 1,000 RPM ...
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bioRxiv - Cancer Biology 2022Quote: The 4T1-mScarlet and 67NR-GFP cell lines were generated by transfection using a 1:4 ratio of plasmid DNA:FugeneHD reagent (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Single CD3−CD8−CD14−CD16−CD20+Ova−Gamma NTD-PE+Wuhan-Hu-1 NTD-AF647+ B cells were sorted into individual wells of 96-well plates containing 4 μl of lysis buffer (0.5× PBS, 10 mM DTT, 3,000 units/ml RNasin Ribonuclease Inhibitors (Promega, N2615) per well using a FACS Aria III and FACSDiva software (Becton Dickinson ...
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bioRxiv - Cell Biology 2022Quote: ... 4 hours post-transfection media was replaced with fresh complete DMEM containing 646 HALO Dye (20 nM; GA112A; Promega). The next day ...
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bioRxiv - Developmental Biology 2019Quote: ... Ark -/- MEFs were transfected with 4 guide pairs in order to create the desired deletion using FUGENE (Promega, E2311) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... or day 4 in TZM-bl cells by measuring luciferase activity using the luciferase assay system (Promega, Madison, WI) and the microplate luminometer Monolight 3096 (BD Biosciences ...
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bioRxiv - Plant Biology 2020Quote: ... template cDNA samples were prepared using 4 κg of total RNA and the Improm-II reverse transcription system (Promega). Primers were designed to have a Tm of ~60°C and to produce PCR products of ~200−400 base pairs ...
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bioRxiv - Developmental Biology 2020Quote: ... Tryptic digests were performed overnight after addition of 46 μl of 100 mM Hepes pH 7.6 and 4 μl of 1 μg/μl Trypsin Gold (Promega).
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were pelleted at 20,000 × g and washed with ice cold 10 mM HCl / 90% acetone before resuspension in 60 μL 4 M Urea and 0.5 × ProteaseMax (Promega), vortexed ...
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bioRxiv - Cell Biology 2021Quote: ... at room temperature (r.t.) for 4 hours and then further digested overnight with 1:50 (w/w) trypsin (Promega) at r.t ...
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bioRxiv - Biochemistry 2021Quote: ... per well of a 96-well plate 4×104 Drosophila SR2+ cells were seeded and transfected using Fugene (Promega) according to the manufacturer’s instructions with the following plasmids ...
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bioRxiv - Immunology 2021Quote: ... Single CD3−CD8−CD14−CD16−CD20+Ova−RBD-PE+RBD-AF647+ B cells were sorted into individual wells of 96-well plates containing 4 μl of lysis buffer (0.5× PBS, 10 mM DTT, 3,000 units/ml RNasin Ribonuclease Inhibitors (Promega, N2615) per well using a FACS Aria III and FACSDiva software (Becton Dickinson ...
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bioRxiv - Microbiology 2019Quote: ... Digestion product was cooled and transferred to a pre-chilled 15 ml tube containing 4 ml 1.1X Ligase Buffer (Promega) and 1% Triton X-100 ...
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bioRxiv - Cell Biology 2020Quote: ... were added alone or in combination at the indicated concentrations (0.25-4 pmol) to in vitro translation reactions (Promega SP6-TNT Quick rabbit reticulocyte lysate system ...
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bioRxiv - Molecular Biology 2022Quote: ... beads were incubated in 400 μl 50 mM ammonium bicarbonate containing 4 μl of sequencing-grade modified trypsin (Promega) at 37 °C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... Viability of MV-4-11 cells was assessed using the CellTiterGlo 2.0 kit from Promega (as per manufacturer instructions); viability of HPAFII cells was assessed using PrestoBlue reagent from Thermo Fisher (as per manufacturer instructions) ...
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bioRxiv - Neuroscience 2024Quote: ... the medium was removed and substituted with OptiMEM media supplemented with 4 µM coelenterazine-H (Promega; Cat. No. S2001), and incubated for a period of 2 to 3 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... The released peptides were then transferred over to a new tube and a 4 h digest using 0.5 ug mass spectrometry-grade trypsin (V5111, Promega) at 37 C for 4 hours was conducted ...
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bioRxiv - Neuroscience 2023Quote: ... after which the slice pieces were fixed in 4% PFA in PBS (with 0.1 U/ml RNase inhibitor, Promega) for 15 mins and dissociated into single cells through careful trituration ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cell viability was assessed in uninfected PBMC incubated with each PROTAC under the same conditions (1.0 µM, 4 d) using the CellTiter-Blue assay (Promega).
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bioRxiv - Cell Biology 2023Quote: The Foxo1/3/4 responsive luciferase reporter plasmid was constructed by inserting six FoxO REs (FoxO-responsive elements) into the pGL4.23 (Promega). The following sequence was used for the FoxO RE ...
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bioRxiv - Immunology 2022Quote: ... Live single Zombie-NIR−CD14−CD16−CD3−CD8−CD20+Ova−N-loop-PE+N-loop- AF647+ B cells were single-cell sorted into 96-well plates containing 4 μl of lysis buffer (0.5× PBS, 10 mM DTT, 3,000 units/ml RNasin Ribonuclease Inhibitors [Promega, N2615]) per well using a FACS Aria III ...
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bioRxiv - Cell Biology 2023Quote: ... 4 hours post-transfection media was replaced with fresh complete DMEM containing JF646 HaloTag ligand (20 nM; GA112A; Promega) when required ...
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bioRxiv - Immunology 2022Quote: ... Live single Zombie-NIR−CD14−CD16−CD3−CD8−CD20+Ova−peptide-PE+peptide-AF647+ B cells were single-cell sorted into 96-well plates containing 4 μl of lysis buffer (0.5× PBS, 10 mM DTT, 3,000 units/ml RNasin Ribonuclease Inhibitors [Promega, N2615]) per well using a FACS Aria III ...
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bioRxiv - Biochemistry 2023Quote: ... 10 µL of 1:100 (diluted in phenyl red-free DMEM with 4% FBS) Nano-Glo substrate (Promega N1572) was added in each well ...
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bioRxiv - Plant Biology 2023Quote: ... Two µl of cDNA (1:4 dilution) was added to a 20µl qPCR reaction using the GoTaq qPCR Master Mix (Promega) and ran on a BioRad CFX Opus96 thermocycler ...
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bioRxiv - Neuroscience 2024Quote: ... Proteolytic digestion was performed by consecutive digestion with LysC (0.1 µg; 4 h) and trypsin (0.1 µg; 16 h) at room temperature (Promega, Germany).
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bioRxiv - Molecular Biology 2024Quote: ... pH 8.0 to an urea concentration of 4 M and proteins were digested with 40 µg endopeptidase LysC (Promega) (1/250 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The plasmid DNA was purified from 4-mL overnight cultures by use of the Wizard Plus MiniPrep kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was then incubated at 37°C for overnight trypsin digestion (trypsin 1:50 protein w/w ratio; Promega, Madison, WI). The digestion reaction was quenched by adding formic acid (FA ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μg of pCS2-HA-NFATc3 was incubated for 2 h at 30°C in 50 μl of the TNT® SP6 coupled wheat germ extract system (Promega, #L5030), according to the instructions of the manufacturer ...
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bioRxiv - Biochemistry 2020Quote: ... Trypsinisation was carried out by incubating the gel pieces overnight at 37 °C in 50 µl of trypsin (1: 25:: trypsin: protein, 20 µg/ml, sequencing grade trypsin V5111, Promega, Madison, WI) prepared in ammonium bicarbonate (50 mM) ...
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bioRxiv - Molecular Biology 2021Quote: ... protein ratio of 1:50 w/w for 2 hours at 37°C and further digested with 30 µL 50 mM TEAB containing trypsin (Sequencing Grade Modified, Promega, Madison, WI) at a trypsin ...
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bioRxiv - Immunology 2021Quote: ... and bound proteins were subjected to on-column digestion using mass spec grade Trypsin/Lys-C Rapid digestion enzyme (Promega, Madison, WI) at 70 °C for 2 h ...
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bioRxiv - Genomics 2019Quote: ... cells were incubated at 37°C with MTS for 90 min following the instruction of CellTiter 96 AQueous One Solution Reagent (Promega, Madison, USA). The absorbance was measured at the wavelength of 490 nm by a Synergy H1 plate reader (BioTek ...
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bioRxiv - Microbiology 2019Quote: ... In-gel digestion of selected protein bands or spots was performed overnight at 37 °C by incubation with trypsin (Sequencing grade, Promega, Madison, USA). Afterwards peptides were extracted as previously described [24] and samples were vacuum-dried using CentriVap Vacuum Concentrator (Labconco) ...
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bioRxiv - Microbiology 2021Quote: ... Growth curves were obtained in 96 wells microplates at 37°C and automatic recording of OD600 every 30 minutes using a plate reader (Promega GloMax Explorer).
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bioRxiv - Cancer Biology 2019Quote: Mitochondrial activity of C-33A cells was measured with the CellTiter 96 Aqueous One Solution Cell Proliferation Assay (MTS) (Promega, Madison, WI). Cells were seeded in 96-well plates (5000 cells/well ...
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bioRxiv - Microbiology 2022Quote: ... The light production was then monitored at 20-minute intervals at 37 °C for 2 hours using the Glomax 20/20 Luminometer (Promega, Madison, WI). A final measurement was also taken after the samples had been allowed to incubate overnight at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then incubated for 24 hours at 37°C in 5% CO2 and the luciferase activity was measured using the ONE-Glo™ luciferase assay (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The cells were incubated at 37°C in 5% CO2 for 24 hours and luciferase activity was measured using a ONE-Glo™ luciferase assay (Promega, USA) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... for 30 min at 37°C with shaking at 500 rpm in 500 μl of PBS with RNasin® Ribonuclease Inhibitors (Promega, #N261B). Negative control samples comprised 5 μg of RNA combined with HisPur Cobalt Resin (see details below) ...
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bioRxiv - Microbiology 2021Quote: ... The CaptureSelect Biotin anti-C-tag Conjugate (LifeTechnologies #7103252100; 1/4000) was mixed with Streptavidin Alkaline Phosphatase (Promega #V5591; 1/1000 dilution) for 10 minutes before use ...
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bioRxiv - Neuroscience 2022Quote: ... we transferred the beads to 100 µL 50 mM Tris-HCl buffer with mixed with 1 µg trypsin/Lys-C (Promega Cat# V5073) per replicate ...
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bioRxiv - Systems Biology 2022Quote: ... 102.2 µL of 100 mM ABC was added and proteins were digested for 16 hours at 37°C using 5 µg of trypsin (dissolved in trypsin resuspension buffer, Promega, Walldorf, Germany). Tryptic digestion was stopped by addition of 2.5 µL 100% formic acid ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were incubated with chemical treatments for 18 hours at 37°C prior to rinsing with PBS and lysing with 1x cell lysis buffer (Promega, Madison, WI). Luciferase activity was measured using a Berthold Centro XS3 LB 960 microplate luminometer with automatic injection of Luciferase Assay Reagent (Promega) ...
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bioRxiv - Molecular Biology 2020Quote: ... The band was cut and washed before the in-gel digestion of the proteins overnight at 37°C with a solution of modified trypsin (sequence grade, Promega, Charbonnières, France). The resulting peptides were extracted from the gel by one round of incubation (15 min ...
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bioRxiv - Microbiology 2019Quote: ... we replaced the C-terminal V5 tag with either HaloTag or NanoLuc (NLuc) from the NanoBRET Nano-Glo Detection System (Promega, Madison, WI). As a negative control ...
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bioRxiv - Synthetic Biology 2020Quote: ... and the bound proteins were subjected to on-cartridge digestion with mass spec grade Trypsin/Lys-C Rapid digestion enzyme (Promega, Madison, WI) at 70°C for 2h ...
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bioRxiv - Molecular Biology 2021Quote: ... cooled on ice to room temperature for 5 min and digested overnight at 37 °C with 0.5 μg of sequencing-grade trypsin (Promega Cat. No. V5111). Peptide mixtures were acidified to pH 3 with 1.5 µl 5% FA ...