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Citations for Promega :
2551 - 2600 of 3835 citations for 5 Nitro 1H benzo de isoquinoline 1 3 2H dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... and digested overnight with a 1:50 ratio of protein:trypsin (Promega, sequencing grade). Detergents were removed through organic-aqueous phase extraction with ethyl acetate ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 1 µL RNase One (10 units/µL RNase ONE Ribonuclease solution, Promega) for 1-4 hours at 37 °C ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 1 µL RNase One (10 units/µL RNase ONE Ribonuclease solution, Promega) for 1 or 2 hours at 30 °C ...
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bioRxiv - Microbiology 2024Quote: ... horseradish peroxidase-conjugated anti-mouse IgG or anti-rabbit IgG (1:25000; Promega) was reacted for 3 h ...
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bioRxiv - Molecular Biology 2024Quote: ... Trypsin 1:50 (w/w) (Gold Mass Spectrometry grade, Promega, Madison, WI, USA) was added to each sample for proteolytic digestion and left incubating overnight at 37°C ...
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bioRxiv - Physiology 2024Quote: ... Digestion was performed using LysC & Trypsin (Promega, 1:20 protease to protein ratio) for 1 hour (37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... before loading each well with 40 µL NanoGlo substrate (1:100 dilution, Promega) and luminescence read on a Glomax plate reader at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... 1% P/S (post-infection DMEM) and 6µM Renilla luciferase substrate (EnduRen, Promega). The mean relative light units (RLUs ...
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bioRxiv - Neuroscience 2024Quote: ... The following antibodies were used: mouse anti–βIII-tubulin (1:2,000; Promega, G7121) and rabbit anti-synaptotagmin-2 (1:500 ...
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bioRxiv - Genomics 2024Quote: ... 100 ng of the fragmented DNA was spiked with 1% lambda DNA (Promega) and subjected to bisulfite conversion using EZ DNA Methylation Lighting Kit (Zymo Research Corp. ...
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bioRxiv - Synthetic Biology 2024Quote: ... The anti-HaloTag antibody, (G921A, 1:500 for WB) was purchased from Promega. The Alexa Fluor™ 488 Goat anti-Mouse IgG (A11029 ...
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bioRxiv - Cell Biology 2024Quote: ... protein extracts were digested overnight with 1 µg of trypsin (Promega, Madison, Wisconsin) in a solution containing 10 mM Tris-HCl at pH 8.0 and 2 mM CaCl2 ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 µg RNA was transcribed using the Reverse Transcription System kit (Promega, UK). Quantitative PCR (qPCR ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 1X GoScript RT Mix for 1-step RT-qPCR (Promega, Fitchburg, WI, USA) as well as 800 nM of each primer and 300 nM of probe ...
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bioRxiv - Genetics 2024Quote: ... 1 µg of total RNA was treated with RQ1 RNase-free DNase (Promega) and retrotranscribed using the iScript™ cDNA Synthesis Kit (Bio-Rad) ...
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bioRxiv - Cancer Biology 2021Quote: ... at 1:166 to Opti-MEM media in combination with extracellular NanoLuc Inhibitor (Promega) diluted 1:500 was combined to create a 3X stock solution ...
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bioRxiv - Cell Biology 2020Quote: ... We used the following primary antibodies: mouse anti-β-galactosidase (Promega, 1:1,000 dilution), rabbit anti-GFP (MBL,1:1,000 dilution) ...
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The key features of SARS-CoV-2 leader and NSP1 required for viral escape of NSP1-mediated repressionbioRxiv - Molecular Biology 2021Quote: ... 100 ng of Trypsin Gold and 1 μg of Trypsin sequenced grade (both Promega) were added to BioID eluates and total cell lysates ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and in bioluminescence mode while immersed in NanoGlo substrate (diluted 1:50, Promega #N1110). Tissues were homogenized in 1 ml N-PER (brain ...
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bioRxiv - Molecular Biology 2022Quote: ... The proteins were digested in 1 M urea with modified trypsin (Promega, Madison, WI) at a 1:50 enzyme-to-substrate ratio ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were then harvested and lysed with 25μL of 1×passive lysis buffer (Promega) for 30 min at room temperature ...
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bioRxiv - Genetics 2021Quote: ... 1 μg linearized vector was purified with the PureYield Plasmid Miniprep System (Promega, A1223) and used as template for in vitro synthesis of capped mRNA with the Ambion mMessage Machine T3 Transcription Kit (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... USA) and 10 μl of GoTaq 1-Step RT-qPCR (Promega, Madison, WI, USA). Thermal cycling was performed at 50°C for 15min for reverse transcription ...
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bioRxiv - Microbiology 2021Quote: ... diluted 1:2 with PBS and read on a GloMax Multi+ Detection System (Promega). CSV files were exported onto a USB flash drive for analysis.
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR was performed using the GoTaq® 1-Step RT-qPCR System (Promega). Knockdowns were verified using the primers ...
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bioRxiv - Biochemistry 2020Quote: ... Proteomic digestion was performed by the addition of 1 μg proteomics-grade Trypsin (Promega) and incubated overnight at 37 °C ...
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bioRxiv - Biophysics 2021Quote: ... 20μL of a 1/5th diluted solution of Bright-Glo reagent (Promega, Madison, WI) was added directly to the wells ...
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bioRxiv - Neuroscience 2021Quote: ... USA) at 1:10 dilution and polyclonal HaloTag antibody (Promega Corp. Madison, WI, USA) at 1:500 dilution and FITC-conjugated donkey anti-mouse secondary antibody and Cy3-conjugated goat anti-rabbit secondary antibody (Invitrogen).
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bioRxiv - Cell Biology 2021Quote: ... 9 μl purified RNA was incubated with 1 μl Oligo(dT)15 primers (Promega) at 80°C for 3 min ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by an overnight trypsin digestion (ratio 1:33 enzyme:lysate, Promega, Madison, WI, USA).
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bioRxiv - Cancer Biology 2020Quote: ... Plates were incubated for 1 h at RT and luminescence recorded (Glowmax Luminometer, Promega) and then compared to Glucose/Lactate standards to determine relevant concentrations.
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bioRxiv - Plant Biology 2021Quote: ... ground into powder and dissolved in 200 μL 1 × Cell Culture Lysis Reagent (Promega), followed by centrifugation for 15 min at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... 40 ng ml-1 of Renilla luciferase under a constitutive promoter (pRL-TK, Promega), and 40 ng ml-1 of pLEXm vector (34 ...
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bioRxiv - Systems Biology 2020Quote: ... The transfections were performed with 1 μg DNA: 2 μl Fugene HD (Promega E2311) ratio ...
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bioRxiv - Genetics 2021Quote: ... 1 μg linearized vector was purified with the PureYield Plasmid Miniprep System (Promega, A1223) and used as template for in vitro synthesis of capped mRNA with the Ambion mMessage Machine T3 Transcription Kit (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... Afterwards 40 µL digestion buffer (1:10 diluted Rapid Digest buffer (Promega, Fitchburg, WI) in water ...
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bioRxiv - Microbiology 2021Quote: ... incubated for 1 h at RT and analysed using the GloMax multidetection system (Promega). Parasitaemia was used to normalise parasite density between samples and replicates ...
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bioRxiv - Microbiology 2021Quote: ... cDNAs were synthesized from 1 μg of total RNA using AMV reverse transcriptase (Promega) and used directly for quantitative PCR (qPCR ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 μg of mass spectrometry grade trypsin/Lys-C mix (Promega CAT# V5071) in 60 μl of 50 mM ammonium bicarbonate was added to each sample ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were digested at 37 °C overnight with trypsin (Promega; 1:10, enzyme/substrate) in the presence of 10% acetonitrile ...
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bioRxiv - Neuroscience 2020Quote: ... overnight at 4°C and HRP-conjugated anti-rabbit antibody (1:2,000, W4011, Promega) for 1 hr at room temperature followed by ECL signal amplification and chemiluminescence detection (SuperSignal West Pico Chemiluminescent Substrate ...
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bioRxiv - Microbiology 2022Quote: ... diluted 1:2 with PBS and read on a GloMax Multi+ Detection System (Promega).
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bioRxiv - Systems Biology 2022Quote: ... and RNA (1.5μg) was treated with RQ1 Dnase I (Promega; 1 U/µg RNA). cDNA was synthesized with a qPCRBIO high-quality cDNA synthesis kit ...
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bioRxiv - Microbiology 2022Quote: ... cDNAs were synthesized from 1 μg of total RNA using AMV reverse transcriptase (Promega) and used directly for quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2022Quote: ... Digestion was performed with trypsin in a ratio of 1:20 m:m protein:trypsin (Promega, P/N V5072 for the pilot study ...
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bioRxiv - Microbiology 2020Quote: ... cDNAs were synthesized from 1 μg of total RNA using AMV reverse transcriptase (Promega), and used directly for quantitative PCR (qPCR ...
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bioRxiv - Molecular Biology 2020Quote: ... Transfected cells were lysed in 12.6 μl of 1 × Passive Lysis Buffer (PLB, Promega) and light emission was measured following injection of 25 μl of either Renilla or Firefly luciferase substrate ...
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bioRxiv - Neuroscience 2021Quote: ... Cells used for growth cone analyses were stained for βIII-tubulin (1:1000, Promega), Phalloidin 568 conjugate (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μg cellular RNA was treated with DNase (RQ1 Rnase-Free DNase kit Promega) and reverse-transcribed following the GoScript ™ Reverse Transcriptase Kit (Promega ...
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bioRxiv - Microbiology 2020Quote: ... which were destained and overnight-digested with trypsin (1 μg/mL, sequencing grade, Promega), before MS analysis of the peptide mixes ...