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Citations for Promega :
2451 - 2500 of 8541 citations for Nitrite Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... and luciferase expression data (ONE-Glo Luciferase Assay, Promega) were collected 24 h post-treatment using a microplate reader ...
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bioRxiv - Biochemistry 2024Quote: BacTiter-Glo Microbial Cell Viability Assay (Promega catalog #G8230) was used to compare total cellular ATP in WT and GndA mutant lines between growth at 37 °C and heat shock conditions ...
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bioRxiv - Microbiology 2024Quote: ... 2 μl of Nano-Glo Luciferase Assay Reagent (Promega) and 18 μl ddH2O were added to induce luminescence development ...
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bioRxiv - Microbiology 2024Quote: ... the CellTiter-Glo luminescent cell viability assay (Promega, #G7571) according to the manufacturer’s instructions was performed and the luminescent was measured by using an Orion II microplate Luminometer (Berthold ...
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bioRxiv - Cancer Biology 2024Quote: ... Cellular viability was determined using CellTiter-Glo assay (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... cells were lysed and subjected to luciferase assays (Promega). Results were expressed as NLRs calculated as follows ...
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bioRxiv - Microbiology 2024Quote: ... using Bright-Glo™ Luciferase Assay System (E2610, Promega) according to the manufacturer’s instruction.
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bioRxiv - Immunology 2024Quote: ... cells were lysed in Steady-Glo Luciferase Assay (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Lysates were loaded in triplicate to 96-well microtiter plates and luminescence was measured in a GloMax 96 microplate luminometer (Promega). Gene expression was measured by firefly luciferase activity (LAR II ...
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bioRxiv - Synthetic Biology 2021Quote: ... The 10 μl of crude extract were mixed with 40 μl of LARII and the FLuc activity was measured by a GloMax 96 Microplate Luminometer (Promega), setting a 2-seconds delay and a 10-seconds measurement ...
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bioRxiv - Cancer Biology 2020Quote: ... 20 μL of cell lysate was transferred to 96-well plates and placed in a 96-well microplate luminometer (GloMax-Multi, Promega). 100 μL Luciferase Assay Reagent II and 100 μL Stop & Glo Reagent (both provided in the Dual-Luciferase Reporter kit ...
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bioRxiv - Microbiology 2022Quote: ... Fluorescent signal (excitation at 475 nm, emission at 500-550 nm) was measured with a microplate reader (Glomax Discover, Promega).
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bioRxiv - Microbiology 2022Quote: ... both OD600nm and fluorescent signal (excitation at 475 nm, emission at 500-550 nm) were measured with a microplate reader (Glomax Discover, Promega).
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bioRxiv - Microbiology 2022Quote: ... was mixed 1:1 with cells seeded in 96-well plates for three consecutive days and the luminescence signal was quantified using the GloMax-Multi microplate reader (Promega). A list of all used sgRNA sequences and genotyping primers can be found in Table S4.
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bioRxiv - Microbiology 2022Quote: ... and the Gaussia princeps luciferase enzymatic activity was measured on a Centro XS LB960 microplate luminometer (Berthold Technologies, reading time 10 s after injection of 50 µl Renilla luciferase reagent (Promega). For the minigenome assays ...
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bioRxiv - Microbiology 2020Quote: ... NanoLuc luciferase activity was measured using a Modulus II Microplate Multimode reader (Turner BioSystem) or a Glowmax Navigator luminometer (Promega), using 0.1s integration time ...
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bioRxiv - Microbiology 2021Quote: ... and the luminescent signal was measured using a CentroXS3 plate reader (Berthhold Technologies) or GloMax explorer multimode microplate reader 3500 (Promega).
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bioRxiv - Cancer Biology 2021Quote: ... lactate assays were performed with culture media collected from each sample according to the manufacturer’s protocol and the optical density was measured at 450 nm using a microplate reader (GloMax®-Multi E7031, Promega)
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bioRxiv - Synthetic Biology 2021Quote: ... 10 μl of crude extract were mixed with 40 μl of LARII and Firefly luciferase (FLuc) activity was determined using a GloMax 96 microplate luminometer (Promega) with a 2-s delay and a 10-s measurement time ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were harvested 48 hours post transfection and Firefly and Renilla luciferase intensities were measured using Glomax 96 microplate luminometer (Promega) and dual-Luciferase Reporter Assay System (Promega).
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bioRxiv - Genetics 2020Quote: ... Cell lysates were assayed in triplicate in a 96-well plate and luciferase activity was quantified using a GloMax microplate luminometer (Promega). Percent frameshift was calculated by averaging the three firefly or Renilla luciferase technical replicate reads per sample then forming a ratio of firefly to Renilla luminescence per sample ...
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bioRxiv - Microbiology 2021Quote: Luminescence measurements were performed using percoll-enriched infected erythrocytes in 384 well microplates using the Nano-Glo HiBiT extracellular detection system (Promega). Cells were resuspended at 0.2% hematocrit in culture medium diluted with two volumes of 200:1:50 buffer:LgBiT:Furimazine ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were seeded in triplo in opaque 96-well plates and cell viability was measured 96h later using the GloMax Discover Microplate reader (Promega). Luciferase reporter assay (Promega ...
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bioRxiv - Neuroscience 2022Quote: ... the absorbance was measured at 450 nm (CCK-8) or 560 nm (MTT) using a microplate reader (GloMax explorer multimode, Promega).
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bioRxiv - Microbiology 2022Quote: ... Cell lysates were analyzed 24 h after transfection to measure firefly and renilla luciferase activities using GloMax 96 microplate luminometer (Promega).
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bioRxiv - Neuroscience 2024Quote: ... were calculated as the ratio of the fluorescence intensities between the initial (F0) and the last (F) 100 seconds by R-CEPIA1er in the Glomax Discover Microplate Reader (Promega). Caffeine-induced Ca2+ transients were also measured with Fura2 AM at room temperature using an ECLIPSE Ti/L100 epifluorescence microscope (Nikon ...
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bioRxiv - Neuroscience 2023Quote: ... washed with PBS and fluorescence intensity was measured by using the microplate reader GloMax fluorimeter (Promega Corporation, Madison, WI, USA) at the excitation wavelength of 475 nm and emission wavelength 555 nm ...
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bioRxiv - Microbiology 2023Quote: ... princeps luciferase enzymatic activity due to luciferase reconstitution was measured on a Centro XS LB960 microplate luminometer (Berthold Technologies) using a reading time of 10 s after injection of 50 µl Renilla luciferase reagent (Promega). Mean relative light units (RLUs ...
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bioRxiv - Immunology 2023Quote: ... The reaction was stopped by KPL TBM Stop Solution (SeraCare, #5150-0021) and plates were read at 450nm on GloMax Explorer Multimode Microplate Reader (Promega).
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bioRxiv - Cell Biology 2023Quote: ... Intracellular fluorescence was monitored after 2 and 3 h of nanoalgosome incubation by spectrofluorimetric analysis using a microplate reader (Glomax, Promega). As vehicle-control ...
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bioRxiv - Microbiology 2023Quote: ... and the luminescent signal produced by firefly luciferase reaction was measured using a GloMax explorer multimode microplate reader 3500 (Promega) or CentroXS3 LB960 (Berthhold Technologies) ...
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bioRxiv - Microbiology 2024Quote: ... The transfected cells were then lysed and analyzed for firefly and renilla luciferase activities using a GloMax 96 microplate luminometer (Promega). The function of ANP32 was examined using a polymerase assay by co-transfection of DKO cells with different ANP32 proteins or empty vector (20 ng ...
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bioRxiv - Molecular Biology 2024Quote: ... EGFP and dsRed accumulation was measured by western blot (see below) and by fluorescence emission of extracts using a GloMax microplate reader (Promega). In both cases ...
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bioRxiv - Microbiology 2021Quote: ... The kinase activity assay was performed with 4 µl beads in a 384-well plate using the ADP-Glo™ Kinase Assay (Promega, Madison, WI). The luminescence of each well was read using a Synergy™ H1 Hybrid Multi-Mode Microplate Reader (BioTek Instruments ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: The evaluation of the cytotoxicity of NBCoV small molecules in the different cell types was performed in parallel with the antiviral activity assay and measured by using the colorimetric CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) (Promega, Madison, WI) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... Cell viability at 72-h incubation was measured by MTS cell proliferation colorimetric assay (CellTiter 96® AQueous One Solution Cell Proliferation Assay system, Promega, G3582, Italy). Cell numbers at 0 ...
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bioRxiv - Cancer Biology 2019Quote: ... a proliferation rate was calculated using the data obtained from MTT colorimetric assays (CellTiter 96® AQueous One Solution Cell Proliferation Assay; Promega; Madison, WI). Data from five replicates for at each time point were analyzed using Graphpad Prism software with a p value less than 0.05 considered significant.
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bioRxiv - Cancer Biology 2019Quote: ... 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay (Celltiter 96 Aqueous One-solution Cell Proliferation Assay, Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... the viability of HAEs was assessed by a MTS assay (CellTiter 96® AQueous One Solution Cell Proliferation Assay; Promega, Madison, WI, USA) by addition of 20 µl of MTS solution with 180 µl of Opti-MEM on the apical pole of HAEs ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The cytotoxicity of NBCoV small molecules in the different cell types was performed in parallel with the neutralization assay and evaluated using the colorimetric CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) (Promega, Madison, WI) following the manufacturer’s instructions ...
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Mucociliary Clearance Augmenting Drugs Block SARS-Cov-2 Replication in Human Airway Epithelial CellsbioRxiv - Microbiology 2023Quote: The cytotoxicity of all compounds (except ARINA-1 that interferes with the assay) was measured using the CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, Cat. # G1780), which measures LDH enzyme release ...
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bioRxiv - Cell Biology 2023Quote: ... Amounts of LDH in conditioned media were assessed by using an ultrasensitive bioluminescent plate-based assay (LDH-Glo™ Cytotoxicity Assay, Promega, Madison, WI). We followed the manufacturer’s instructions in their entirety and used a Molecular Devices SpectraMax M5 (San Jose ...
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bioRxiv - Cancer Biology 2023Quote: Cell viability assays were performed using the RealTime-Glo™ MT Cell Viability Assay according to the manufacturer’s protocol (Promega, Madison, WI, USA). Cells were seeded in standard media in white-walled 96-well plates at a density of 5000 cells/well and were allowed to attach overnight ...
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bioRxiv - Immunology 2023Quote: ... prepared by combining ONE-Glo™ EX Luciferase Assay Buffer with ONE-Glo™ EX Luciferase Assay Substrate in 1:1 ratio (Promega, USA). After measuring the signal of the Firefly luciferase in the GloMax® 20/20 Luminometer (Promega ...
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bioRxiv - Immunology 2023Quote: ... cells were further cultivated for 36 h and harvested for luciferase assay using the Nano-Glo luciferase assay system (Promega; cat. no. N1120) and the GloMax Navigator Microplate Luminometer (Promega) ...
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bioRxiv - Immunology 2024Quote: Cytokine or chemical inhibitor doses were selected based on available EC50 data and toxicity profiles with untreated macrophages (Trypan Blue staining or MTS assay; CellTiter 96 Aqueous One Solution Cell Proliferation Assay Promega, Madison, WI, USA). Only inhibitor doses resulting in >90% cell viability were utilized ...
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bioRxiv - Microbiology 2019Quote: ... Relative growth was determined using luciferase levels measured at 0 h (initial setup values) and after 72 h using the GloMax® Discover Multimode Microplate Reader (Promega). Luminescence values were normalized to samples treated with a lethal dose of chloroquine (200 nM ...
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bioRxiv - Cell Biology 2024Quote: ... fluorescence intensity was measured using a fluorescence plate reader with an excitation wavelength of 485 nm and an emission wavelength of 538 nm (GloMax® Discover Microplate Reader, Promega). The relative percentage of intracellular ROS was normalized respect to untreated cells (control).
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bioRxiv - Microbiology 2023Quote: ... In Ghent the optical density was measured by diluting the culture in phosphate-buffered saline in a 96-well microtiter plate and measuring the turbidity in a plate reader at 600 nm (GloMax® Explorer Multimode Microplate Reader, Promega).
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bioRxiv - Genetics 2023Quote: ... The activity of the firefly and renilla for each sample were measured with a Promega GloMax® 96 Microplate Luminometer (Promega). Per well ...