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Citations for Promega :
2451 - 2500 of 4570 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade C TV019 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... mixed 1 to 1 (v / v) with 10 μg / ml trypsin (Sequencing Grade Modified Trypsin, Promega) in 50 mM ammonium bicarbonate and incubated for 2 hours at 60 °C for enzymatic hydrolysis ...
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bioRxiv - Immunology 2024Quote: ... organoids were collected and lysed in 100 µl of a 1:1 dilution containing CellTiterGlo (Promega) and PBS ...
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bioRxiv - Immunology 2022Quote: Caspase-1 activity was quantified using a Caspase-Glo 1 Inflammasome Assay kit (Promega, Madison, WI). THP-1 cells were treated and harvested as described above ...
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bioRxiv - Immunology 2023Quote: ... then combined in a 1:1 ratio with Nano-Glo® Luciferase solution (Promega, Ref N1150). A Promega GloMax plate reader was used to measure luminescence ...
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bioRxiv - Cell Biology 2023Quote: ... Top half was incubated in anti-Nup107 (1:1000; ab236634) and HaloTag (1:500; Promega G9211). Bottom half was incubated in anti-GAPDH (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... organoids were stained using Hoechst 33342 (1 μg/mL) and CellTox Green (Promega, 1/20 000) dyes for 8 h prior to imaging at Opera Phenix (Perkin Elmer ...
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bioRxiv - Cell Biology 2023Quote: ... For Non-SILAC samples, 1 µl trypsin (1 µg/µl Trypsin Gold, Mass Spectrometry Grade (Promega)) was added and samples were incubated at 37°C overnight ...
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bioRxiv - Immunology 2023Quote: ... then combined in a 1:1 ratio with Nano-Glo® Luciferase solution (Promega, Ref N1150). A Promega GloMax plate reader was used to measure luminescence in technical triplicate for each sample ...
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bioRxiv - Genetics 2023Quote: ... and for 1 hour at room temperature in secondary antibody (1:4,000 anti-Mouse-HRP, Promega). In order to detect the endogenous Ptiwi09 protein ...
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bioRxiv - Plant Biology 2024Quote: ... Aliquots #1 and #2 were then treated with 1 mg/mL trypsin (Promega, Madison, WI, USA) and incubated at 37 °C for 1 h followed by the addition of 1.5 mg/mL trypsin inhibitor (Worthington Biochemical Corp ...
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bioRxiv - Microbiology 2024Quote: Released caspase-1 enzymatic activity of was quantitated with the Caspase-Glo 1 inflammasome assay (Promega), following instructions from the supplier ...
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bioRxiv - Biochemistry 2021Quote: ... Pull down assays were performed utilizing myc-tagged proteins that were generated from recombinant pGBKT7-derivatives through in vitro transcription/translation using wheat germ extract (cat# L4330, Promega, Madison, WI, USA)(Stephan et al. ...
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bioRxiv - Plant Biology 2021Quote: ... In vitro transcription and translation to radiolabel the proteins with [35S]-methionine was performed with the TnT® Quick Coupled Transcription/Translation System (Promega, Walldorf, Germany) in reticulocyte lysate ...
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bioRxiv - Microbiology 2021Quote: ... HepG2 or sg25 cells were transfected with a control plasmid or protein expression plasmids together with the luciferase reporter plasmids using Viafect TM Transfection Reagent (Promega, Madison, WI, USA) or Lipofectamine TM 2000 (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... The protein mixture was diluted to 25mg/ml with 50mM ABC and 500ug of protein were digested with 2% w/w Trypsin (Promega, Cat. no. V5111) in 1mL of 50mM ABC overnight at 37°C ...
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bioRxiv - Biochemistry 2021Quote: The kinase activity of the FLT3-ITD protein was measured by using ADP-Glo™ Kinase Assay Kit (Promega, Madison, Wisconsin, the US). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunoprecipitated protein–DNA complexes and 5% input were analyzed by qRT-PCR using GO taq QPCR master mix (Promega, A6002, lot no. 0000385100) in triplicate using primers specific for PFKFB3 HREs ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting proteins with carbamidomethyl groups on their cysteines were digested with 300 ng of sequencing-grade porcine trypsin (Promega, Fitchburg, MA, USA) and injected on an Easy-nanoLC-1000 system coupled to a Q-Exactive+ mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: The cell free protein expression assays were performed by using the TnT® Quick Coupled Transcription/Translation System (Cat#L1171, Promega, Madison, USA), according to the manufacturer’s guideline with minor modification ...
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bioRxiv - Molecular Biology 2023Quote: SMC5/6 hexamer purified from E.coli with a C-terminal HALO tag on Nse2 was incubated with a 2-fold molar excess of Janelia-Fluor646 HaloTag Ligand (Promega; 12 µM Smc5/6 hexamer + 24 µM label). After incubation for 1 h at 25°C ...
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bioRxiv - Genetics 2021Quote: ... 0.5 μL 1% Digitonin (Promega, G9441), 22 μL H2O ...
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bioRxiv - Molecular Biology 2021Quote: ... Size markers were 1 kb (Promega) and λ-HindIII (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μM HaloTag-TMR substrate (Promega), 0.25 U/μL Benzonase (Merck) ...
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bioRxiv - Cell Biology 2022Quote: ... 1 U/μl RNasin Plus (Promega)) ...
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bioRxiv - Cell Biology 2022Quote: ... 1 U/μl RNasin Plus (Promega) in 2x Saline-sodium citrate (SSC ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of modified trypsin (Promega) was added ...
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bioRxiv - Genomics 2020Quote: ... 1% RNasin Plus RNase Inhibitor (Promega) was included in all buffers ...
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Domain-specific quantification of prion protein in cerebrospinal fluid by targeted mass spectrometrybioRxiv - Neuroscience 2019Quote: ... 1 µg of trypsin (Promega V5113) was added (final concentration of ∼1.4 ng/µL) ...
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bioRxiv - Immunology 2019Quote: ... 0.5 µl of 1% digitonin (Promega), 22 µl nuclease-free water ...
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bioRxiv - Molecular Biology 2021Quote: ... 1% NLuc substrate (Nano-Glo, Promega), 16 pg/μl NLuc-mRNA and 0.6 μM Nsp1 protein or its mutants/domains or eIF1A as negative control ...
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bioRxiv - Biochemistry 2021Quote: ... and 1 μl dNTP (Promega, U1515) in NEBuffer 3 in a 50 μl reaction at 37°C for 60 min (25) ...
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bioRxiv - Systems Biology 2020Quote: ... donor (1 mM UDP-GlcNAc (Promega) for B3GNT2 ...
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bioRxiv - Microbiology 2021Quote: ... and 1 U DNase RQ1 (Promega) and incubated at 37°C for 15 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μg of trypsin (Promega, # V5111) was then added to the samples and proteolysis was carried out overnight ...
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bioRxiv - Biochemistry 2022Quote: ... 25:1 (w/w) chymotrypsin (Promega), or 10:1 (w/w ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 μL Pfu DNA Polymerase (Promega), and 200 μM dNTP mix (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 µl dNTPs mix (Promega #U1515), 5 µl 10X DreamTaq Buffer (ThermoFisher #EP0703) ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM DTT and RNasin (Promega) 4U/ml ...
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bioRxiv - Microbiology 2019Quote: ... 1 mg/mL Proteinase K (Promega) with 0.5% SDS in DPBS at 40 °C overnight ...
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bioRxiv - Microbiology 2021Quote: ... 1 mg/mL Proteinase K (Promega) with 0.5% SDS in PBS at 40 °C overnight ...
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bioRxiv - Biochemistry 2021Quote: ... Halo (Promega G9211, dilution 1:10000), SETD2 (Abclonal A3194 ...
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bioRxiv - Cancer Biology 2022Quote: ... or AP 1-driven luciferase (Promega) reporter plasmids ...
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bioRxiv - Microbiology 2022Quote: ... 1 unit of GoTaq enzyme (Promega) and 25 pmoles of pan-coronavirus primers (10) ...
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bioRxiv - Developmental Biology 2019Quote: ... 1 U RNAse inhibitor (RNAsin, Promega) and 5 U reverse transcriptase for 60’ at 42°C ...
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bioRxiv - Biochemistry 2019Quote: ... Proteomics grade trypsin (1 µg; Promega) was added to each sample ...
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bioRxiv - Developmental Biology 2019Quote: ... mouse anti-LacZ (1/1000; Promega), mouse anti-βPS (1/10 ...
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bioRxiv - Biochemistry 2020Quote: ... 7.5:1 (w/w) Chymotrypsin (Promega) or 5:1 (w/w ...
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bioRxiv - Microbiology 2021Quote: ... 1× GoTaq reaction buffer (Promega, USA), deoxynucleoside triphosphate (dNTP ...
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bioRxiv - Biophysics 2019Quote: 50ml 1% agarose (analytical grade, Promega) gels pre-stained with SYBR safe (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: ... and 1 unit/mL RNAsin (Promega)) at room temperature for 30 minutes (unless otherwise noted) ...