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Citations for Promega :
201 - 250 of 1815 citations for Ribonuclease P Protein Subunit p38 RPP38 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Aliquots were then transferred to SAM2 biotin capture membrane (Promega), which was washed ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were detected with secondary peroxidase-conjugated antibodies (Promega, Madison, WI, USA) and ECL ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Stimulation of P-gP ATPase activity was utilized to indicate the interaction between peptides and P-gP using the P-gP-Glo Assay System (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Antibodies were obtained in carrier/ protein-free buffer or were purified using the Magne Protein A or G Beads (Promega) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... then transfected with pRK5-based constructs of iGluR subunits and Neto variants (1 µg total DNA/ ml cells) using ViaFect transfection reagent (Promega). The cells were incubated at 37°C for 3 hours ...
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bioRxiv - Immunology 2022Quote: ... and p MD.2G using Fugene 6 (Promega, PAE2693) in HEK293T cells ...
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bioRxiv - Microbiology 2020Quote: ... Promega)/ 0.33 mg/ml NBT (p-nitroblue tetrazolium chloride, Promega) containing solution (100 mM Tris pH 9.5 ...
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bioRxiv - Systems Biology 2023Quote: ... + P/S + 8 ng/mL rhFGF-basic (Promega #G5071) + 2 ug/mL doxycycline ...
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bioRxiv - Cell Biology 2023Quote: ... Anti-p-JNK (pTPpY, Promega, USA-1:100 dilution); 8 ...
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bioRxiv - Plant Biology 2021Quote: ... Protein production was confirmed by western blot using an anti-HaloTag antibody (Promega). HaloTag-ligand conjugated magnetic beads (Promega ...
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bioRxiv - Physiology 2022Quote: ... 1.15 μg of purified AQP2 or AQP2 S256A COOH-tail peptide were mixed with either RSK (16 nmol) or PKA catalytic subunit (5.6 nmol) (Promega, Madison, WI) in a total volume of 12 μl reaction buffer containing 40 mM Tris pH 7.5 ...
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Noradrenergic alpha-2A receptor activation suppresses courtship vocalization in male Japanese quail.bioRxiv - Animal Behavior and Cognition 2021Quote: ... or p-GEM-t easy vectors (Promega, Madison, WI, USA). The plasmids were digested with the XhoI ...
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bioRxiv - Neuroscience 2020Quote: ... the RNA-bound beads were washed four times in 900 μL of 0.35 M KCl (10 mM HEPES [pH 7.4], 350 mM KCl, 10 mM MgCl2, 1% NP40, 2 mM DTT, 100 U/mL RNasin® Ribonuclease Inhibitors [Promega, N2111], and 100 μg/mL cycloheximide). During the final wash ...
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bioRxiv - Neuroscience 2020Quote: ... the RNA-bound beads were washed four times in 900μL of 0.35M KCl (10mM HEPES [pH 7.4], 350 mM KCl, 10 mM MgCl2, 1% NP40, 2 mM DTT, 100 U/mL RNasin® Ribonuclease Inhibitors [Promega, N2111], and 100 μg/mL cycloheximide). During the final wash ...
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bioRxiv - Neuroscience 2020Quote: ... the RNA-bound beads were washed four times in 900μL of 0.35M KCl (10mM HEPES [pH 7.4], 350 mM KCl, 10 mM MgCl2, 1% NP40, 2 mM DTT, 100 U/mL RNasin® Ribonuclease Inhibitors [Promega, N2111], and 100 μg/mL cycloheximide). During the final wash ...
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bioRxiv - Microbiology 2023Quote: ... Biotin-oligo d(T) (Promega, #Z5261, 0.2 μmol/L for mRNA FISH); Diethyl Pyrocarbonate (DEPC)-treated water (Invitrogen™ ...
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bioRxiv - Biochemistry 2020Quote: ... the biotin-streptavidin roadblock was prepared by adding streptavidin monomer (Promega, Madison, WI) to 100 nM ...
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bioRxiv - Molecular Biology 2020Quote: ... Beads-biotin-probes-RNA adducts were captured by magnets (Promega, Madison, WI, USA) and washed five times with the Wash buffer (2X SSC ...
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bioRxiv - Genetics 2022Quote: ... and directionally cloned into the pGL3-Promoter plasmid (pGL3-P) (Promega) upstream of the SV40 promoter using restriction enzymes ...
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bioRxiv - Systems Biology 2020Quote: ... + P/S + 0.5μg/mL Puromycin + 16ng/mL rhFGF-basic (Promega G5071).
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bioRxiv - Microbiology 2020Quote: ... and cloned into a P-Gem T plasmid (Promega Corporation, U.S.A.). The plasmids were linearized with the restriction enzyme SacII ...
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bioRxiv - Microbiology 2022Quote: ... FLAG-tagged or HA-tagged protein was detected using a commercially available mouse monoclonal antibody (Promega).
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bioRxiv - Microbiology 2020Quote: ... The peptides were spotted onto streptavidin-coated filter sheets (Promega SAM2 biotin capture membrane) and visualized by phosphorimaging on Typhoon FLA 7000 ...
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bioRxiv - Immunology 2021Quote: ... RBD and NTD antigens were site-specifically biotinylated using Halotag PEG biotin ligand (Promega), following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... cells were subjected to a pulse with 5 µM biotin-HaloTag ligand (G828A, Promega) diluted in complete medium for 3 hours ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HaloTag Alexa Fluor 488 ligand (G1001) and HaloTag Biotin ligand (G8281) were from Promega, USA ...
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bioRxiv - Systems Biology 2023Quote: ... + P/S + 0.5ug/mL puromycin + 16 ng/mL rhFGF-basic (Promega #G5071). When passaging hiF-T cells ...
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bioRxiv - Plant Biology 2022Quote: ... Protein expression was confirmed by Western blot with Anti-Halo Tag monoclonal antibody (1:2000) (G9211, Promega).
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bioRxiv - Biophysics 2024Quote: ... we also performed a control experiment by blocking the HaloTag with HaloTag Biotin Ligand (Promega). Bacteria in 100 μL sucrose buffer were first incubated with 2.5 μM HaloTag Biotin Ligand (stock stored at -20 °C in DMSO ...
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bioRxiv - Immunology 2024Quote: ... env (P-COLT-GALV) and pBu-CD20–CAR using FugeneHD transfection reagent (Promega). Human peripheral blood mononuclear cells (PBMCs ...
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bioRxiv - Microbiology 2024Quote: ... 1% P/S (post-infection DMEM) and 6µM Renilla luciferase substrate (EnduRen, Promega). The mean relative light units (RLUs ...
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bioRxiv - Neuroscience 2022Quote: ... Protein bands were detected with horseradish peroxidase (HRP)-conjugated rabbit or mouse secondary antibody [1:1,000 dilution] (Promega). The chemiluminescence signal was imaged using an Odyssey XF Imager (LI-COR ...
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bioRxiv - Biophysics 2021Quote: ... Biotin-containing handles were made with a PCR on pBluescript SK+ (stratagene) with GoTaqG2 (Promega, M7845), in the presence of 1/5 biotin-16-dUTP (JenaBioscience ...
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bioRxiv - Microbiology 2022Quote: ... Reactions were performed at 1/5th volume with the inclusion of Transcend Biotin-Lysyl-tRNA (Promega) to allow antibody independent detection of all translated products ...
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bioRxiv - Microbiology 2020Quote: ... A-tailed PCR amplicons were cloned into p-GEM-T Easy Vector System (Promega) per manufacturer instructions and ligation reactions introduced directly into electrocompetent XL1-Blue E ...
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bioRxiv - Developmental Biology 2021Quote: ... The gel-purified fragment was cloned into the p-GEM-T Easy vector (Promega) and digoxigenin-labelled probes generated by in vitro transcription using a DIG RNA labelling kit (Roche).
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bioRxiv - Immunology 2021Quote: ... The antibodies were purified from the culture supernatant 4 – 6 days later using protein G magnetic beads (Promega #G7472). Purified antibodies and antibody elution buffer (5 parts elution buffer (100 µM glycine-HCl ...
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bioRxiv - Cell Biology 2022Quote: ... 4 μl of each reaction was spotted onto a streptavidin-coated SAM2 Biotin Capture Membrane (Promega, #TB547). The membrane was air-dried ...
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bioRxiv - Bioengineering 2024Quote: ... with the luciferin conjugated specific CYP3A (1:40) substrate (P450 P-Glo Luminescence Kit, Promega) at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Proteins separated on the second gel were transferred to a PVDF membrane and DivJ levels were measured using Western blots by probing the protein with anti-HaloTag antibody (Promega). Due to the requirement of growing cells in low phosphate media ...
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bioRxiv - Synthetic Biology 2021Quote: ... Proteins were transferred by semi-dry Western Blot onto PVDF membranes visualised with polyclonal antibody and BCIP/NBT (Promega, S3771).
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bioRxiv - Molecular Biology 2022Quote: ... Expression of Halo-bZIP and SBP-bZIP proteins were confirmed by western blotting using anti-HaloTag monoclonal antibody (Promega, G9211) and anti-SBPTag mouse anti-human antibody (MilliporeSigma ...
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bioRxiv - Biochemistry 2023Quote: ... The expression of FgSte3-NLuc donor protein produced was also validated performing immunoblotting using anti-Nluc antibody (Promega cat#7000), see details below.
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bioRxiv - Immunology 2024Quote: ... 2 µg of anti-DNA-PKcs antibody or the corresponding IgG control was incubated with 70 ng of recombinant DNA-PKcs protein (Promega) in 500 µL of lysis buffer (20 mM Tris–HCl [pH 7.4] ...
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bioRxiv - Biochemistry 2024Quote: ... The luminescence NAD(P)H-Glo™ Detection System (assay kit) was purchased from Promega (USA). Protein Thermal Shift™ Dye Kit (no ...
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bioRxiv - Molecular Biology 2023Quote: ... Western blots were run according to standard procedure and probed with streptavidin IRDye680RD (Licor 925-68079) to track biotinylated HaloTag protein and anti-HaloTag antibody (Promega G928A) to track total HaloTag protein ...
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bioRxiv - Molecular Biology 2023Quote: Western blots were run according to standard procedure and probed with streptavidin IRDye680RD (Licor 925-68079to track biotinylated HaloTag protein and anti-HaloTag antibody (Promega G928A) to track total HaloTag protein ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were then digested with LysC at 1ug/100ug protein (Promega) for 3 h at room temperature (RT ...
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bioRxiv - Plant Biology 2020Quote: ... Fusion proteins were purified using MagnetHis™ protein purification system (Promega) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2024Quote: ... Proteins were precipitated through the addition of Protein Precipitation Solution (Promega) followed by vigorous vortexing ...