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Citations for Promega :
201 - 250 of 546 citations for IL 12 Mouse HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... and anti-mouse IgG HRP conjugate (Promega, W4021) as the secondary antibody ...
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bioRxiv - Biochemistry 2023Quote: ... anti-mouse-IgG-HRP (W402B, 1:8000, Promega); anti-mouse-IgM-HRP (SC2064 ...
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bioRxiv - Genetics 2023Quote: ... mouse anti-β-galactosidase (Promega Z378A; 1:500), rabbit anti-pH3 (Millipore #06-570 ...
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bioRxiv - Cell Biology 2023Quote: ... goat anti-mouse (Promega, W4021, dilution 1:3000) and rabbit anti-goat (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... mouse anti-1-gal (1:1000, Promega Z378B), mouse anti-1-gal (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... mouse monoclonal antibIII tubulin (1:500, Promega, #G712A), mouse anti-tubulin bIII (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... mouse anti-beta galactosidase (Promega Z378825580610; 1:500), guinea pig anti-brk (provided by from Gines Morata ...
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bioRxiv - Physiology 2023Quote: ... HRP-conjugated anti-mouse antibodies (Promega, Cat#W4021) in TBST was added to membranes and rocked on a rotator for 1 hour at the room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... or anti-mouse IgG (Promega, W4021, 1:5000) and developed using enhanced chemiluminescence (ECL ...
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bioRxiv - Immunology 2024Quote: ... Anti-Mouse IgG HRP (1:20,000 Promega W402B), Anti-Rabbit IgG HRP (1:20,000 Promega W401B) ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-βIII Tubulin (1: 1,000, Promega, G712A), chicken anti-βIII Tubulin (1:1,000 ...
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bioRxiv - Molecular Biology 2024Quote: ... and anti-mouse-HRP 1:10000 (S3721, Promega). The guinea-pig anti-MCTS1 antibodies (used at 1:500 ...
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bioRxiv - Developmental Biology 2021Quote: ... media was collected at a single timepoint (12 hours) and the Promega Glutamate/Glutamine-Glo kit (Promega cat. no. J8021) was used ...
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bioRxiv - Molecular Biology 2019Quote: ... Nanoluciferase was immunoprecipitated by addition of 12 μg of rabbit polyclonal anti-Nluc IgG (a generous gift from Lance Encell, Promega) or normal rabbit IgG as a negative control (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2019Quote: ... Transfections were performed with 4 μg of DNA, 800 μL of OptiMEM (Invitrogen, and 12 μL of FuGENE 6 transfection reagent (Promega) per 10 cm dish ...
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bioRxiv - Biochemistry 2019Quote: JAK2-deficient ϒ2A human fibrosarcoma cells were transfected with different human JAK2-hemagglutinin (HA) constructs in pCIneo vector (100 ng per 12-well plate well) with FuGENE HD (Promega). After 48 hours ...
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bioRxiv - Molecular Biology 2019Quote: ... qPCR amplifications (12 μl) were done in duplicates using 1 μl of cDNA and the GoTaq Probe 2X Master Mix (Promega) on a LightCycler 480 (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... The sequences ligated by the T4 ligase at 4 °C for 12 h were cloned in the pGEM T-easy vector (Promega). The competent Escherichia coli X10-Gold (Stratagene ...
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bioRxiv - Molecular Biology 2020Quote: ... phaffii cells were cultured for 12-14 h and total RNA was isolated using RNA isolation kit (Cat. # Z3100, Promega) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... air-dried and treated by 10 mkl of 12 mg/mL solution of trypsin (Trypsin Gold, Mass Spectrometry Grade, Promega) in 50 mM ammonium bicarbonate for 15 h at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... were stacked in the top of a 12% SDS-PAGE resolving gel before in-gel digestion using modified trypsin (sequencing grade, Promega), as previously described (Casabona et al. ...
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bioRxiv - Genomics 2020Quote: ... Viral RNA was extracted using the silica guanidinium isothiocyanate binding method (12) adapted for the ThermoFisher Kingfisher using paramagnetic silica particles (Magnesil, Promega).
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bioRxiv - Cell Biology 2021Quote: ... BOSC 23 retroviral packaging cells were ∼70% confluent when transfected with 12 µg of pBABE-mPPARγ2 or pBABE-EGFP plasmid DNA using Fugene 6 transfection reagent (Promega, E2311). Media containing secreted retrovirus was collected 72 hours post-transfection and filtered through 0.45 µm syringe filters ...
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bioRxiv - Microbiology 2021Quote: ... from 12-72 hpt and luciferase activity was measured using the Dual Luciferase reagent kit and GloMax multi detection system (Promega). Firefly luciferase readings from the TBEV replicons were normalized to the Renilla values (pTK-Ren ...
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bioRxiv - Biochemistry 2021Quote: ... Digestion was performed by adding 50 mM NH4HCO3 with 12 ng/μl sequencing-grade modified trypsin (Promega, Madison, WI, USA), incubation on ice for 4 h before overnight incubation at 37 °C ...
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bioRxiv - Genomics 2019Quote: Neutrophils were plated at the desired cell numbers and treated with 25nM phorbol 12-myristate 13-acetate (PMA, Promega V1171) or co-cultured in the presence of E ...
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bioRxiv - Genomics 2021Quote: ... 8 μg of RNA were treated for 1 h at 37°C with 12 units of RQ1 DNase (Promega®) followed by phenol-chloroform extraction and ethanol precipitation of RNA which was subsequently dissolved in water ...
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bioRxiv - Immunology 2022Quote: Samples were resuspended in 100µl to a final concentration of 200mM HEPES and 12 ng/µl of trypsin gold (Promega, V5280). Samples were then placed in a bioshaker (Bulldog Bio ...
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bioRxiv - Evolutionary Biology 2022Quote: ... or RepNDK-RNA was incubated at 37 °C for 12 h in a translation system and FluoroTect GreenLys tRNA (Promega), with neither CTP nor CDP to preclude RNA replication ...
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bioRxiv - Developmental Biology 2022Quote: ... in a 12 µl mixture consisting of 6 µl Blue SYBR Green mix (Biozym) or GOTaq qPCR Master Mix (Promega), 2 µl primer pairs (2.5 µM each ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Wells containing single cell colonies were duplicated into 12-well plates and screened for HiBit expression by mixing crude lysates with purified LgBit protein (Promega) and measuring luminescence in the presence of 5 μM furimazine ...
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bioRxiv - Microbiology 2022Quote: ... The plates were incubated at 37°C for ~12 hours before luciferase activity was quantified using the Luciferase Assay System (Promega) using SoftMax Pro v.7.0.2 (Molecular Devices) ...
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bioRxiv - Biochemistry 2023Quote: Approximately 0.1 million cells/well of a 12-well plate were seeded with HEK293T cell line and transfected after 70% confluency using a Lipofectamine reagent (Promega). For the cycloheximide assay ...
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bioRxiv - Biochemistry 2023Quote: ... The gel pieces were dehydrated using 100% ACN before 25 µl of 12 ng/µl sequence grade modified trypsin porcine (Promega) in 25 mM ABC was added ...
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bioRxiv - Molecular Biology 2023Quote: ... gel pieces were dried and subsequently rehydrated in solution containing 12 ng/μL Trypsin Gold (mass spectrometry grade from Promega), 0.01% ProteaseMAX surfactant (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells was grown at 12-well plates for 18h and transfected with MCHR1 constructs and the cAMP biosensor GloSensor-22F (Promega) at a ratio of 3:2 ...
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bioRxiv - Cell Biology 2023Quote: ... cells grown to ∼70% confluence in 6-well plates and co-transfected with a gRNA to TBC1D23 (12) and pmaxGFP using Fugene6 (Promega) according to manufacturer’s instruction ...
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bioRxiv - Cell Biology 2023Quote: ... cells were plated on three 10 cm tissue culture dishes per condition and transfected 24 h before lysis using FuGENE 6 (6-12 μg total plasmid DNA; Promega). Published protocol can be found on Protocols.io (dx.doi.org/10.17504/protocols.io.kxygx3zeog8j/v1).
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bioRxiv - Neuroscience 2024Quote: Nuclear fractions from Con (n=13) and DLB (n=12) were pretreated with DNAse (50 u/ml, 15 mins RT, RQ1 DNAse, Promega), were adjusted to 80 ng /µl with LDS sample buffer (Fisher Scientific) ...
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bioRxiv - Genetics 2024Quote: ... HEK293T cells cultured in a 12-well plate were harvested and processed according to the instruction of Dual-Glo Luciferase Assay System (Promega) with minor modification ...
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bioRxiv - Cell Biology 2024Quote: ... then transfected with 4 μg pLenti-CMV-PuroDEST-ERK-KTR-mClover DNA plus 12 μL FuGENE 6 (Promega Cat. #E2691) in 800 μL of Opti-MEM (Thermo-Fisher Cat ...
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bioRxiv - Genetics 2021Quote: ... cell supernatant from untransfected and cells transfected with IL2 constructs was diluted 1:12 with complete media and used to perform the IL2 Bioassay (Promega, JA2201). Luminescence (RLU ...
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bioRxiv - Cancer Biology 2021Quote: ... treated with LD50 concentrations of 10058-F4 or CB-839 for 12 h in DMEM without FBS then analyzed by Glutamate-Glo™ Assay (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Media was refreshed on day 6 and plates collected on day 12 with cell viability quantified by CellTiter-Glo 3D (Promega #G9681). Dose response assay analysis was performed as described above.
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bioRxiv - Microbiology 2020Quote: ... transfection experiments) or 4×105 cells (12 well format; IFN experiments) were lysed in 120 μl passive lysis buffer (Promega #E1941). Half the lysate was used to quantify luciferase protein (Promega Luciferase Assay System #E1501 ...
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bioRxiv - Immunology 2020Quote: ... Single CD19+IgG+RBD+ cells were sorted into a 96 well plate containing 4 µL lysis buffer (12 units of RNasin Ribonuclease Inhibitor (Promega, N2511), 10 mM DTT and PBS x0.5 ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was diluted 5-fold with 50 mM Tris-HCl pH 8.0 and digested for 12 h at 37°C using sequencing grade trypsin (Promega cat#: V511A) at 1/100 the mass of total protein ...
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bioRxiv - Genomics 2023Quote: ... The concentration of 12 wells (diagonal) of the plate containing the diluted cDNA was checked using the QuantiFluor dsDNA System (Promega, E2670) fluorometric assay as the initial cDNA concentration is a critical step to obtain libraries with adequate fragment length ...
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bioRxiv - Immunology 2023Quote: ... cells were transfected with 12 µg of plasmid expressing the spike from various variants using Fugene-6 (Promega; catalog number: E2691) according to the manufacturer’s instructions and incubated at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... and P5Solexa and P3Solexa primers (Supplementary Data 12) on the whole eluted volume and a second ProNEX purification step (Promega, NG2001) to remove residual primers ...