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Citations for Promega :
201 - 250 of 2706 citations for 8 N N Diethylamino octan 1 ol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Relative cell viability was determined using a Cell counting kit-8 (Dojindo) and read on a microplate reader (Promega).
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bioRxiv - Molecular Biology 2021Quote: ... Digestion was performed for 8 h at 37°C using 70 ng modified porcine trypsin (Promega, Fitchburg, WI, USA). Tryptic peptides were extracted using 70% acetonitrile and dried using a SpeedVac vacuum concentrator.
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bioRxiv - Neuroscience 2023Quote: ... Equilibrated cells were transfected with 0.8 μg of 5-HT1eR or 5-HT1FR and 8 μg of GloSensor plasmid (Promega), after mixing with 17.6 μl of PEI in OptiMEM (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were diluted using one volume of dilution buffer (10 mM Tris pH 8, 0.4% NP40, 5 mM CaCl2, 2 U/mL RQ1 DNAse (Promega)) and then incubated with anti-Flag or anti-p400 antibodies coupled to agarose beads (Sigma ...
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bioRxiv - Plant Biology 2020Quote: ... following the manufacturer’s instructions (eluted into 8 μl of TLE) before A-tailing in a reaction containing 2 μl of GoTaq buffer (Promega), 0.2 μl 10 mM ATP ...
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bioRxiv - Microbiology 2020Quote: ... post-transfection with or without OSM (10 ng/mL for 8 h) or IFN-α (1,000 U/ml for 16 hours) before lysis using Passive Lysis Buffer (Promega). Firefly and Renilla luciferase activity was then determined in a dual luciferase assay ...
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bioRxiv - Neuroscience 2020Quote: ... 8 min, 4 °C) and resuspended in staining buffer (PBS, pH 7.4, with 0.5% RNase-free BSA (Promega, Heidelberg, Germany) and 0.2 U/μl of RNasin Plus RNase inhibitor (Promega)) ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were then diluted to 6 M urea with 50 mM Tris-HCl pH 8 and Trypsin/Lys-C mix (Promega) was added to an enzyme:substrate ratio of 1:25 (w/w ...
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bioRxiv - Cell Biology 2022Quote: ... The bound proteins were digested by resuspending the beads in 200 μL freshly prepared digestion buffer (2M Urea, 100 mM Tris-HCl pH 8, 100 mM DTT) with 0.5 ug trypsin (Promega, V5111) and overnight incubation at room temperature while shaking ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were then diluted to 4 M urea with 50 mM Tris-HCl pH 8 and Trypsin/Lys-C mix (Promega) was added to an enzyme:substrate ratio of 1:250 (w/w ...
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bioRxiv - Immunology 2019Quote: 8 nM cap0 or 20 ng/μL VPg-linked RNA was translated using the Flexi Rabbit Reticulocyte Lysate system (Promega) in the presence or absence of 1.5 μM Ifit proteins ...
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bioRxiv - Bioengineering 2019Quote: ... and 8-oxo-2’-deoxyguanosine-5’-triphosphate (8-oxo-dGTP) respectively,52 both in combination with the non-proofreading Taq DNA polymerase (GoTaq, Promega). The primers used for this PCR reaction anneal outside the His6-SpAS1 open reading frame (Table S8) ...
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bioRxiv - Molecular Biology 2020Quote: ... RL and FL activities were measured on 96-well plates using 2 µl of cell extract containing 8 µl of 1x PLB (Promega) and the dual-luciferase assay kit (Promega) ...
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bioRxiv - Systems Biology 2021Quote: ... In 18 hours cells were stimulated for 8 hours with a corresponding inducer and harvested in the passive lysis buffer (Promega). Luminescence of Firefly and Renilla luciferases was measured using dual-luciferase-reporter assay (Promega ...
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bioRxiv - Immunology 2019Quote: ... Samples were diluted 10-fold with 50 mM Triethylammonium bicarbonate buffer at pH 8 and incubated overnight at 37°C with sequencing grade trypsin (Promega) added at a 1:50 enzyme:substrate ratio (wt/wt).
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bioRxiv - Cell Biology 2019Quote: ... Samples were diluted 10-fold with 50 mM triethylammonium bicarbonate buffer at pH 8 and incubated overnight at 37°C with sequencing grade trypsin (Promega) at a 1:50 enzyme:substrate ratio (wt/wt).
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bioRxiv - Cancer Biology 2021Quote: ... Cell lysates were prepared by addition of 0.3N hydrochloric acid then 450nM Tris (pH=8) and metabolites were analyzed using the Glutamine/Glutamate Glo Assay (Promega; J8021) per manufacturer’s instruction.
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bioRxiv - Molecular Biology 2021Quote: ... the coding sequence of REST/NRSF ZF1-8 were amplified by PCR using cDNA as templates and cloned into the pTNT vector (Promega) between the EcoRI and XbaI sites ...
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bioRxiv - Immunology 2021Quote: ... cells were transfected with one of the Gag-mCherry variants (8 µg) using FuGene HD according to the manufacturer’s instructions (Promega, USA) in a 3:1 ratio with DNA ...
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bioRxiv - Developmental Biology 2022Quote: ... Luciferase assays were conducted 8 hours after stimulation using a dual luciferase kit (Dual-Luciferase® Reporter Assay System, Promega) according to manufacturer’s protocol.
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bioRxiv - Neuroscience 2022Quote: ... the absorbance was measured at 450 nm (CCK-8) or 560 nm (MTT) using a microplate reader (GloMax explorer multimode, Promega).
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bioRxiv - Biochemistry 2022Quote: ... Luciferase expression was measured at 8 hr (for translation) and 72 hr (for replication) using the Renilla Luciferase Assay System (Promega). Renilla luciferase luminescence was measured using a Clariostar Microplate Reader (BMG Labtech ...
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bioRxiv - Cell Biology 2022Quote: ... and the -1680 bp to +8 bp and -1525/-1495 element mutant were constructed in the pGL3-Basic luciferase reporter vector (Promega) using standard molecular biology techniques ...
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bioRxiv - Neuroscience 2022Quote: ... Digestion of proteins in the gel pieces was done by covering them with a trypsin solution containing (8 ng/µL sequencing-grade trypsin (Promega), dissolved in 50 mM NH4HCO3with 10% ACN) ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCR Tag analysis was performed either by adding 1.8 μL of yeast lysate used as template DNA to 6.25 μL of 2X GoTaq® Green Master Mix (Promega), 4.75 μL of nuclease-free water and 400 nM of each primer or by adding 1 µl of yeast lysate to 5 µl of DreamTaq Green PCR Master Mix (2X ...
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bioRxiv - Immunology 2022Quote: ... Ectopic Flag-tagged Galectin 8 or HA-tagged LILRB4 plasmid were transfected into the Mc38/B16/HEK294 cells with Fugene (Promega). A blank vector control was applied ...
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bioRxiv - Cancer Biology 2022Quote: ... CaspaseGlo assays were performed in white-walled 96-well plates to measure the activity of caspase 9 (cat. G8210) or caspase 8 (cat. G8200) (both from Promega) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 8 × 105 HEK293T cells/well were seeded into 6-well plates and transfected the next day with ViaFect (Promega, E4981), using either 4 μg of plasmid expressing the G protein or 2 μg of plasmid expressing HA together with 2 μg of plasmid expressing NA ...
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bioRxiv - Microbiology 2023Quote: ... Viral titers were measured by infecting MDCK cells and measuring Nanoluciferase activity 8 h later with the Nano-Glo Luciferase Assay System (Promega)(52).
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bioRxiv - Microbiology 2023Quote: ... In solution digests were performed overnight at pH 8 and 37 °C with mass spectrometry grade trypsin (Promega, Madison, WI) at a 1:33 enzyme-to-protein ratio and quenched with formic acid (0.15%) ...
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bioRxiv - Bioengineering 2023Quote: ... The AAV production media were collected 72 h and 120 h post transfection and concentrated using a PEG-precipitation method with 8% PEG-8000 (wt/vol; Cat# V3011, Promega). The harvested cell pellets were re-suspended and lysed through sonication ...
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bioRxiv - Developmental Biology 2024Quote: ... To quantify the activity of reports groups of 5 embryos were collected 8 hours post-injection in triplicates and lysed in 100 mL of lysis buffer (Promega). Reporter expression was quantified with the Nano-Glo Dual-Luciferase Reporter Assay System (Promega ...
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bioRxiv - Biophysics 2024Quote: ... 8 μl of the terminated reaction mixture were then applied to a streptavidin biotin capture membrane (SAM2, Promega, Cat.# V2861) and further treated following the manufacturer’s recommendations ...
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Caspase-8 Modulates Angiogenesis By Regulating A Cell Death Independent Pathway In Endothelial CellsbioRxiv - Developmental Biology 2019Quote: The Caspase-8 Glo® assay was performed in combination with the CellTiter-Fluor™ Cell Viability Assay (both from Promega) according to the manual ...
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bioRxiv - Cell Biology 2021Quote: ... 30 min) and diluted to 1M urea with 200mM ammonium bicarbonate for trypsin digestion (1μg, 37°C, 8 h, Promega cat # V5113).
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bioRxiv - Synthetic Biology 2022Quote: ... Peptide digestion was initiated by adding 25 μl of 50 mM ammonium bicarbonate buffer (pH 8) containing 2 μg trypsin (Sequencing Grade Modified Trypsin, Promega #V5117) directly on top of the column and incubating overnight at 37 °C in a drying oven ...
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The human sperm basal body is a complex centrosome important for embryo pre-implantation developmentbioRxiv - Developmental Biology 2021Quote: ... The resulting protein extract was first diluted to 2M urea for overnight digestion with LysC (Wako, USA) at 37°C and then diluted 2-fold for 8 h digestion with trypsin (Promega, USA) at 37°C.
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bioRxiv - Neuroscience 2022Quote: To assess the effect of DA and DA-HA on cell viability, CCK-8 (DJDB4000X, Dojindo laboratory) or MTT (CellTiter 96® Non-Radioactive Cell Proliferation Assay, Promega) was used ...
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bioRxiv - Microbiology 2022Quote: Activity of caspases-3/7 and -8 were assessed using the corresponding Caspase-Glo® Assays in white-walled 96-well plates (Promega).
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bioRxiv - Neuroscience 2023Quote: ... was performed by incubating the proteins first with endoproteinase Lys-C (Wako, #125-05061,)) in 8 M urea for 4h at 37°C followed by an overnight trypsin digestion (Promega #V511A,) in 2 M urea at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... Peptide digestion was initiated by adding 25 µL of 50 mM ammonium bicarbonate buffer (pH 8) containing 2 µg trypsin (Sequencing Grade Modified Trypsin, Promega, # V5117) directly on top of the column and incubating overnight at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then resuspended in 30 μL of 8 M Urea and 30 μL of ProteaseMax surfactant (20 μg/mL in 100 mM ammonium bicarbonate, Promega, V2071).
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bioRxiv - Cell Biology 2021Quote: ... The resulting protein samples were diluted to a final concentration of 2M Urea using 20 mM HEPES (pH 8) and digested overnight at 37°C using sequencing grade modified Trypsin (Promega, Madison, WI) in a 1 ...
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bioRxiv - Biophysics 2020Quote: ... P1KO cells were transiently transfected in a 6-well plate (the cells were seeded 8 h before transfection) in the presence of ruthenium red (10 μM) and mouse Piezo1 (3 μg) using Fugene6 (Promega, Madison, WI). 20–30% of cells showed positive GFP expression indicating successful Piezo1 transfection.
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bioRxiv - Evolutionary Biology 2022Quote: ... The samples were diluted with 8 volumes of 25 mM Tris-HCl pH 8.0 followed by addition of sequencing-grade modified Trypsin (Promega Corp., Madison, WI) (0.4 μg/ sample ...
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bioRxiv - Molecular Biology 2023Quote: ... diluted with 4 volumes of 20 mM Tris-HCl pH 8 2 mM CaCl2 prior to overnight digestion with 100 ng sequencing grade trypsin (Promega, Cat# V5111) at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Pseudoviruses capable of single-cycle infection were prepared by transfecting 8×106 HEK293T/17 cells with a proviral backbone plasmid and an envelope expression vector by using FuGENE HD (Promega, Madison, WI) and following the manufacturer’s instructions as previously described46 ...
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bioRxiv - Immunology 2023Quote: ... Urea concentration was further adjusted to 2M using 50mM Tris pH 8 and Lysyl peptides were additionally digested with sequencing grade trypsin (Promega, Madison, WI) in an enzyme/substrate ratio of 1:20 at 37°C overnight ...
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bioRxiv - Microbiology 2024Quote: ... and the three randomly selected variants (AMA22_1 IHCA and AMA23 IHC AVI 4 μg/mL and R AVI 8 μg/mL) was performed following the manufacturer’s instructions using the Wizard Promega kit (Promega, Madison, WI, USA). For whole genome sequencing ...
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bioRxiv - Molecular Biology 2023Quote: HEK293 cells were seeded in 24-well plates at a density of 4 × 104 or 8 × 104 cells/well and co-transfected 24 hours later with 200 ng of the PsiCheck2 plasmid (Promega, cat #C8021) with the human dystrophin 3′ UTR or mutant 3′ UTR and with 50 nM miR-146a mimic (Life Technologies ...