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Citations for Promega :
201 - 250 of 5242 citations for 3 4 Hydroxy 5 isopropyl 6 oxo 1 6 dihydro pyrimidin 2 ylsulfanyl propionic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... The transfection mix was prepared using Fugene 6 (Promega) at 3µl/ug DNA in 500µl (total volume ...
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bioRxiv - Bioengineering 2020Quote: ... and 42 μL FuGENE 6 (Promega, Cat. No. E2691). The tube was gently flicked to mix the plasmids before and after the addition of FuGENE 6 ...
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bioRxiv - Biochemistry 2020Quote: ... Transfections were carried out by using Fugene 6 (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... or FLAG-KrascomQ61R using FuGene 6 reagent (Promega, E2691) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... HEK293 cells were transiently transfected using Fugene 6 (Promega) with GFP-Tnf 3’UTR reporter constructs and a pGL3-mCherry control construct ...
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bioRxiv - Cell Biology 2021Quote: ... Digestion was with 6 ng/μl trypsin (Promega, UK) overnight at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were transfected using FuGENE® 6 reagent (Promega) combined with 12 μg of the indicated plasmid cDNA construct ...
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bioRxiv - Microbiology 2023Quote: ... 6 washes were performed before adding the trypsin (Promega) at 1/20 ratio for 2h at 47°C ...
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bioRxiv - Molecular Biology 2023Quote: Plasmid DNA transfections were performed using FuGENE 6 (Promega), Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transfected using FuGENE 6 (Promega, Madison, WI) or lipofectamine 3000 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... and 18 μl of FuGENE 6 transfection reagent (Promega), according to manufacturer’s recommendations ...
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bioRxiv - Evolutionary Biology 2023Quote: ... was combined with 9.6 μL FUGENE 6 (Promega #E2691) and incubated for 5 minutes ...
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bioRxiv - Immunology 2024Quote: ... and 18 μL of FuGENE 6 transfection reagent (Promega), according to manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... approximately 1×106 cells were plated and transfected with 1 μg of “driver” plasmid (pTMO2F3, p5UThORF2 or other) + 3 μg of “reporter” plasmid (pJBM560) using 12 μL FuGENE® 6 (Promega). Transfection efficiency determination ...
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bioRxiv - Molecular Biology 2024Quote: ... and transfected with 1 μg of “driver” plasmid (pTMO2F3, p5UThORF2 or other “driver”) + 3 μg of “reporter” plasmid (pAluneoTet, or other “reporters”) using 12 μL FuGENE® 6 (Promega); the following day ...
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bioRxiv - Microbiology 2021Quote: ... and the SARS-CoV-2 spike protein (23) into HEK 293T cells using the FuGENE 6 Transfection Reagent (Promega). Spike sequences from the following global strains REF were used ...
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bioRxiv - Cell Biology 2022Quote: ... were transfected with 2 μg of fresh bacmid DNA and 6 μL of FuGene HD transfection reagent (Promega, E2311) according to the manufacture’s instruction ...
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bioRxiv - Bioengineering 2024Quote: ... 10μM of 2-(4-Morpholinyl)-8-phenyl-4 H-1-benzopyran-4-one (Ly294002: Promega, #V120A), 50ng/ml of Activin A (Stemcell Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... These cells were transfected with the pcDNA6.2-GW /EmGFPmiR plasmids containing miR sequences (miR-NRF2 #1, #2, #3, #4 and miR-ctl 79 using the FUGENE HD transfection reagent (Promega #E2311). Seven days later GFP positive cells were isolated by flow cytometry (Biorad S3e cell sorter) ...
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bioRxiv - Cell Biology 2024Quote: ... U2OS cells were transfected with 1 μg of pX330 plasmid and 1 μg of the HDR donor plasmid using 1 ug FuGene 6 (Promega). Approximately 2-3 days post-transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 and 6 d using a CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) (G3582; Promega, Madison, WI). Cells were incubated with the MTS reagent 3-4 h ...
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bioRxiv - Molecular Biology 2024Quote: About 5 μg empty pcDNA3 vector or ZNF410 full-length and ZNF410-ZF plasmids was transiently transfected with FuGENE 6 (Promega) into 10 cm plates of COS-7 cells ...
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bioRxiv - Cell Biology 2022Quote: ... anti-goat alkaline phosphatase-linked antibody and alkaline phosphatase substrate (5-bromo-4-choloro-3-indolyl 1-phosphate and nitroblue tetrazolium) from Promega (Southampton, UK); a hydroxamate-based MMP inhibitor CT-1746 (N1-[2-(S)-(3,3-dimethylbutanamidyl)]-N4-hydroxy-2-(R)-[3-(4-chlorophenyl)-propyl]-succinamide ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were collected 4- and 6-hours post-transfection (hpt) for Luciferase measurement using Dual-Luciferase® Reporter Assay System (Promega) and RNA extraction for RT-qPCR assay using Luna® Universal One-Step RT-qPCR (New England Biolabs ...
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bioRxiv - Bioengineering 2022Quote: ... The DNA transfection reagents were prepared by incubating 3 µg of plasmid DNA encoding the Bxb1 recombinase and 6 µL of Fugene6 reagent (Promega Cat # E2693) in 300 µL of Opti-MEM for 15 minutes and then added to the cell suspension ...
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bioRxiv - Developmental Biology 2022Quote: HEK293T cells were simultaneously transfected with 1 μg pCS2Cachd1-eGFP or pCS2Cachd1_V1122D-eGFP and 1 μg KDEL-tRFP plasmid using Fugene 6 transfection reagent (Promega). eGFP/tRFP expression was confirmed 24 hours post transfection and the cells fixed at 42 hours post transfection and imaged on a spinning disk microscope (see below).
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bioRxiv - Biochemistry 2021Quote: ... HEK293T cells and C3H10T1/2 cells were transfected in 6-well plates with various constructs of pmirGlo vector (Promega®). Transfection was performed using Turbofect transfection reagent (Thermo Fischer scientific® ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293T cells were grown to 80% confluency before transfection with pCMV3-SARS-CoV-2-spike (kindly provided by Dr. Peihui Wang, Shandong University, China) using FuGENE 6 (Promega). Cells were cultured overnight at 37 °C with 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... HEK293T cells were grown to 80% confluency before transfection with pCMV3-SARS-CoV-2-spike (kindly provided by Dr. Peihui Wang, Shandong University, China) using FuGENE 6 (Promega). Cells were cultured overnight at 37°C with 5% CO2 ...
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bioRxiv - Biochemistry 2023Quote: ... 1 × 106 Sf9 cells in 2 mL of media in a 6-well dish were transfected with up to 2 µg of fresh bacmid DNA using FuGene HD transfection reagent (Promega) at a ratio of 3:1 (Fugene reagent:DNA ...
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bioRxiv - Immunology 2023Quote: ... and the corresponding SARS-CoV-2 spike construct were co-transfected in HEK293-T cells using FuGENE 6 Transfection Reagent (Promega) in Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Developmental Biology 2024Quote: ... Transfection for each well was performed with a mixture of 2 µg of DNA and 6 µL of Fugene HD reagent (Promega). The DNA/fugene mixture was incubated for 15 minutes at room temperature prior to its drop-wise addition to the infected culture ...
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bioRxiv - Cell Biology 2024Quote: ... Bacmid DNA was extracted from overnight cultures using isopropanol precipitation as described.72 About 1 × 106 Sf9 cells in 2 mL of media in a 6-well dish were transfected with up to 2 μg of fresh bacmid DNA using FuGene HD transfection reagent (Promega) at a ratio of 3:1 (Fugene reagent:DNA ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... COS-7 cells were plated on 10 cm plates and transfected 24h prior to lysis using FuGENE 6 (Promega; 6-12 µg total DNA). For siRNA tests ...
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bioRxiv - Cell Biology 2023Quote: ... COS-7 cells were plated on 10 cm plates and transfected 24h prior to lysis using FuGENE 6 (Promega; 6-12 µg total DNA). Cells were routinely tested for mycoplasma using a MycoAlert detection kit (Lonza ...
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bioRxiv - Cell Biology 2021Quote: ... per 10 cm dish using Fugene 6 (Catalog# Promega# E2691), and the medium was changed after 8 hours using (IMEDM+10%FBS) ...
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bioRxiv - Microbiology 2020Quote: ... Retroviral particles were generated using the Fugene 6 reagent (Promega) to simultaneously transfect subconfluent monolayers of 293T cells with 1μg pBABE (vector ...
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bioRxiv - Cell Biology 2021Quote: Plasmid and siRNA transfections were done with Fugene 6 (Promega) and Lipofectamine RNAiMAX (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were digested with 6 ng/µL trypsin (Promega, UK) overnight at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa cells were transfected using FuGENE 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Transfections were performed with Fugene 6 transfection reagent (Promega, USA) according to manufacturer’s instructions with minor modifications ...
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bioRxiv - Microbiology 2020Quote: ... and 0.25 μl of the transfection reagent Fugene 6 (Promega). After 20 min incubation at room temperature ...
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bioRxiv - Immunology 2022Quote: ... and luciferase-IRES-ZsGreen using FuGENE 6 Transfection Reagent (Promega). Culture supernatants were exchanged with fresh medium (high glucose DMEM supplemented with 2 mM L-glutamine ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were digested with 6 ng/µL Trypsin (Promega, UK) overnight at 37 °C ...
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bioRxiv - Biophysics 2021Quote: ... Cells were transfected using the FuGENE 6 Transfection Reagent (Promega) at a ratio of 1 μg DNA/3 μL FuGENE 6 according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Cells were transfected with cDNA using FuGene 6 (Promega, E2691) following manufacturer’s guidelines and a ratio of 0.5 µg of plasmid:1.5 µL FuGene 6 (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... 1.5 μl of Fugene 6 transfection reagent (Promega, Madison, WI) and 100 μl of OptiMEM (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were digested with 6 ng/µL Trypsin (Promega, UK) overnight at 37 řC ...
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bioRxiv - Developmental Biology 2023Quote: ... the cells were transfected using FuGENE 6 transfection reagent (Promega). Transfection mixture was prepared in serum-reduced OptiMEM medium (Thermo ...
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bioRxiv - Molecular Biology 2022Quote: ... and 6 ng of Renilla luciferase vector (Promega, Cat. E6921) were co-transfected into HEK 293 cells ...