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Citations for Promega :
2401 - 2450 of 5375 citations for Human Guanylate Binding Protein 6 GBP6 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: The TNT® Quick Coupled Transcription/Translation Kit from Promega was for synthesis of 35S-methionine labeled proteins in reticulocyte lysate ...
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bioRxiv - Cancer Biology 2022Quote: ATP was measured using the Cell Titer Glo kit (Promega). Eight thousand cells were plated into a 96-well plate (Corning ...
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bioRxiv - Evolutionary Biology 2022Quote: ... with the Wizard Genomic DNA Purification Kit (Promega, Madison, WI), according to the procedure provided by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... used for in vitro transcription with the RiboMAX kit (Promega) and the resulting RNA was purified using the NucleoSpin RNA mini kit (Machery-Nagel ...
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Acinar-to-ductal metaplasia in the pancreas requires a glycolytic switch and functional mitochondriabioRxiv - Cancer Biology 2022Quote: ... The isolation was done using the Maxwell Kit (all Promega) according to the manufacture’s protocol.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 20] using the T7 RiboMAXTM Express RNAi kit (Promega, USA) according to manufacturer’s instructions (Supplementary Table 2) ...
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bioRxiv - Biophysics 2023Quote: ... CellTiter-Glo® 3D Cell viability kit (Promega, Madison, WI) was used according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2023Quote: ... The CellTiter-Glo luminescent cell viability assay kit (G9242, Promega) was used to measure cell viability after treatment ...
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bioRxiv - Cancer Biology 2023Quote: ... and with fluorescence-based method using QuantiFluor Dye kit (Promega) and Quantus (Promega ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lines were authenticated using the GenePrint 24 kit (Promega) and DSMZ Online STR Analysis database at the DNA Sequencing Core at the University of Utah ...
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bioRxiv - Plant Biology 2023Quote: ... Luciferase assay was performed by luciferase assay kit (Promega; E152A) as manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... the double luciferase Reporter gene kit was purchased from Promega company ...
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bioRxiv - Cancer Biology 2023Quote: ROS was measured using ROS-Glo H2O2 assay kit (Promega). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... The Dual-Luciferase Reporter Assay System kit was from Promega. Coelenterazine 400a was from Nanolight Technology (Cat# 340).
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bioRxiv - Molecular Biology 2023Quote: ... ATP hydrolysis was then measured using ADP-Glo kit (Promega) by adding 5uL ADP-Glo reagent for 40 minutes followed by 10uL Kinase Detection Reagent for 1hour ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was obtained using an ImPROM II kit (Promega, A3800) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... extracted using the Wizard® Genomic DNA Purification Kit (Promega) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... the BacTiter-Glo microbial cell viability assay kit (Promega, USA) was employed for this purpose ...
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bioRxiv - Cancer Biology 2024Quote: ... Luciferase assays wered performed using the Luciferase System Kit (Promega) and luminescence was measured ...
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bioRxiv - Developmental Biology 2024Quote: ... RiboMAX™ Large Scale SP6 RNA polymerase kit (Promega, #P1280) was used for in vitro transcription on XbaI linearized pCS2+ vectors ...
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bioRxiv - Cancer Biology 2024Quote: ... CellTiter 96 non-radioactive cell proliferation assay kit (Promega, G4000) was used to determine cell growth and the manufacturer’s instructions were followed ...
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bioRxiv - Biochemistry 2024Quote: ... the TNT® Quick Coupled Transcription/Translation Kit from Promega was used ...
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bioRxiv - Neuroscience 2024Quote: ... Luminescence was measured using the Dual Glo Luciferase Kit (Promega) following the manufacturer’s protocol in an ELISA plate reader (Tecan ...
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bioRxiv - Neuroscience 2024Quote: ... using the CytoTox 96® Non-Radioactive assay kit (Promega), according to the manufactureŕs instructions ...
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bioRxiv - Immunology 2024Quote: ... LDH release was assessed using the Cytotoxicity Detection Kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: The biomass recovered from isolate incubation in broth medium was subjected to DNA extraction using the commercial kit Wizard® Genomic DNA Purification Kit (Promega Corporation, Madison, WI, USA), following the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was then incubated at 37°C for overnight trypsin digestion (trypsin 1:50 protein w/w ratio; Promega, Madison, WI). The digestion reaction was quenched by adding formic acid (FA ...
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bioRxiv - Biochemistry 2020Quote: ... Trypsinisation was carried out by incubating the gel pieces overnight at 37 °C in 50 µl of trypsin (1: 25:: trypsin: protein, 20 µg/ml, sequencing grade trypsin V5111, Promega, Madison, WI) prepared in ammonium bicarbonate (50 mM) ...
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bioRxiv - Immunology 2021Quote: ... and bound proteins were subjected to on-column digestion using mass spec grade Trypsin/Lys-C Rapid digestion enzyme (Promega, Madison, WI) at 70 °C for 2 h ...
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bioRxiv - Cell Biology 2019Quote: Full-length BUB-1 and ULP-1 cDNAs were cloned into pF3 WG (BYDV) Flexi® Vectors and expressed using the TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega). BUB-1 reactions included 35[S]-labelled methionine to allow for further detection ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were first digested for 5 h at 37 °C with 500 ng rLys-C Mass Spec Grade (Promega, Madison, WI, USA) before being diluted 4-fold with 100 mM Tris HCl pH 8.5 to reach a concentration below 2 M urea ...
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bioRxiv - Microbiology 2019Quote: ... In-gel digestion of selected protein bands or spots was performed overnight at 37 °C by incubation with trypsin (Sequencing grade, Promega, Madison, USA). Afterwards peptides were extracted as previously described [24] and samples were vacuum-dried using CentriVap Vacuum Concentrator (Labconco) ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were digested with 1 μg lysyl endopeptidase (LysC) (FUJIFILM Wako Pure Chemical Corporation) and 1 μg trypsin (Promega, Tokyo, Japan) overnight at 37°C on a shaking incubator ...
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bioRxiv - Molecular Biology 2021Quote: ... IPs were performed by incubating lysates equivalent to 20 mg total protein supplemented with RNase inhibitor - RNasin (Promega, Germany, Cat. No. N2511) and 1 mM DTT with 80 μl of anti-GFP magnetic beads GFP-Trap (Chromotek ...
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bioRxiv - Systems Biology 2022Quote: ... 102.2 µL of 100 mM ABC was added and proteins were digested for 16 hours at 37°C using 5 µg of trypsin (dissolved in trypsin resuspension buffer, Promega, Walldorf, Germany). Tryptic digestion was stopped by addition of 2.5 µL 100% formic acid ...
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bioRxiv - Cell Biology 2020Quote: ... The medium was then replaced with fresh medium in the incubator and protein release was induced by biotin addition (100 µM final) and following Furmazin (Promega, 1/500) addition ...
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bioRxiv - Molecular Biology 2020Quote: ... The band was cut and washed before the in-gel digestion of the proteins overnight at 37°C with a solution of modified trypsin (sequence grade, Promega, Charbonnières, France). The resulting peptides were extracted from the gel by one round of incubation (15 min ...
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bioRxiv - Synthetic Biology 2020Quote: ... and the bound proteins were subjected to on-cartridge digestion with mass spec grade Trypsin/Lys-C Rapid digestion enzyme (Promega, Madison, WI) at 70°C for 2h ...
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bioRxiv - Developmental Biology 2019Quote: ... the expression of the fusion protein was initiated by washing out Dox and changing the medium into CDMi containing protected furimazine (Promega; 1 μM). After the NLuc signal was confirmed 5-8 hours later ...
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bioRxiv - Biochemistry 2019Quote: ... A total of 100 µg of each protein sample was digested with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, Wisconsin, USA) by using filter-aided sample preparation (FASP ...
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bioRxiv - Molecular Biology 2021Quote: ... and incubated overnight at 25 °C or endoproteinase GluC in a GluC/protein ratio of 1/20 (w/w) (Promega Cat. No.V1651) and incubated overnight at 37 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... protein in the gel pieces was digested using 20 ng/μl of Trypsin solution made in 25mM ammonium bicarbonate (Promega, Madison, WI) by incubating at 37°C overnight ...
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bioRxiv - Plant Biology 2022Quote: ... The pCMX-PL2-NterHA/MED14 vector was constructed as described above and expressed as HA-MED14 proteins using the TNT T7 Coupled Reticulocyte Lysate System (Promega, Madison, WI). HA-MED14 prey proteins were incubated with the affinity-purified GST-PIF4 or GST-HMR bait proteins immobilized on glutathione Sepharose 4B beads (GE Healthcare ...
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bioRxiv - Plant Biology 2022Quote: ... Protein complexes were then expressed in vitro using the TNT SP6 Coupled Wheat Germ Extract System (Promega, Madison, WI, USA, Cat #L4130) and purified using Magne HaloTag Beads (Cat #G7282) ...
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bioRxiv - Physiology 2022Quote: ... protein samples were digested by incubated overnight at 37°C with sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin). Samples were acidified using 5% TFA and peptides cleaned up using the Phoenix 96× kit (PreOmics ...
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bioRxiv - Neuroscience 2022Quote: ... Protein digestion was processed for overnight by adding LysC and trypsin in a 1:50 ratio (µg of the enzyme to µg of protein; Promega; Cat#: V5071)) at 37° C and 1400 rpm ...
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bioRxiv - Systems Biology 2022Quote: ... 102.2 µL of 100 mM TEAB was added and proteins were digested for 16 hours at 37°C using 5 µg of trypsin (dissolved in trypsin resuspension buffer, Promega, Walldorf, Germany). Tryptic digestion was stopped by addition of 2.5 µL 100% formic acid ...
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bioRxiv - Developmental Biology 2022Quote: ... the expression of the fusion protein was initiated by washing out Dox and changing the media into CDMi containing protected furimazine (Promega; 1 μM). After the NLuc signal was confirmed 5-8 hours later ...
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bioRxiv - Neuroscience 2024Quote: ... or shRNA constructs with viral enzymes and envelope proteins (pMDLg/RRE, pRSV-REV and pVSV-G) using FuGENE6 transfection reagent (Promega Cat# E2692). HEK293T media was replaced with Neurobasal media (Thermo Fisher Scientific Cat# 21103049 ...
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bioRxiv - Systems Biology 2023Quote: ... proteins were precipitated with isopropanol50 and digested overnight at 37°C with 1:50 enzyme : protein ratio by Trypsin/Lys-C Mix (Promega GmbH, Germany). Resulting peptides were desalted on MicroSpin column (The Nest Group ...