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Citations for Promega :
2401 - 2450 of 3460 citations for 6H Purin 6 imine 1 ethoxy 7 ethyl 1 7 dihydro 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Guides targeting 5’ and 3’ regions (see primer sequences in Supplementary File 6) of the PIP5Pase coding sequence were produced by in vitro transcription with T7 polymerase (Promega), and co-transfected with recombinant saCas9 Nuclease NLS Protein (Applied Biological Materials Inc.) ...
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bioRxiv - Developmental Biology 2024Quote: ... Transfection for each well was performed with a mixture of 2 µg of DNA and 6 µL of Fugene HD reagent (Promega). The DNA/fugene mixture was incubated for 15 minutes at room temperature prior to its drop-wise addition to the infected culture ...
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bioRxiv - Cell Biology 2024Quote: ... Oligonucleotides encoding guide RNAs targeting M18BP1 (5’- TTGTACTGAAAAAATCATCA-3’) were cloned into pX459-v2 and co-transfected using FuGENE 6 (Promega) with pUC19 containing a 1528 base pair stretch containing the mutated sequence of the locus of interest and homology arms ...
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bioRxiv - Cell Biology 2024Quote: ... then transfected with 4 μg pLenti-CMV-PuroDEST-ERK-KTR-mClover DNA plus 12 μL FuGENE 6 (Promega Cat. #E2691) in 800 μL of Opti-MEM (Thermo-Fisher Cat ...
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bioRxiv - Cell Biology 2024Quote: ... mammalian expression vectors containing the fusion gene under the control of a weak L30 promoter and containing a Neomycin resistance marker were transfected into U2OS cells at 70% confluence using FuGENE 6 (Cat. No. E2691, Promega). Transfected cells were selected with G418 (Cat ...
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bioRxiv - Cancer Biology 2024Quote: ... 500 ng of reporter vector plus 50 ng of the renilla luciferase plasmid was transfected to cells using FuGENE 6 (Promega); cells were then treated with DMSO ...
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bioRxiv - Molecular Biology 2024Quote: ... the cDNA library is pre-amplified in a first PCR (6 cycles) followed by size selection with ProNex beads (Promega) to remove short cDNAs and primer dimers ...
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bioRxiv - Cell Biology 2024Quote: ... Bacmid DNA was extracted from overnight cultures using isopropanol precipitation as described.72 About 1 × 106 Sf9 cells in 2 mL of media in a 6-well dish were transfected with up to 2 μg of fresh bacmid DNA using FuGene HD transfection reagent (Promega) at a ratio of 3:1 (Fugene reagent:DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... EGFP-IRES-BlastR + 3 μg pMDLg/pRRE-Gag & Pol + 1.5 μg pRSV-Rev + 1.5 μg VSV-G envelope + 42 μL Fugene 6 (Promega cat#E2691) + 600 μL serum-free DMEM ...
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bioRxiv - Molecular Biology 2024Quote: ... EGFP-IRES-BlastR + 3 μg pMDLg/pRRE-Gag & Pol + 1.5 μg pRSV-Rev + 1.5 μg VSV-G envelope + 42 μL Fugene 6 (Promega cat#E2691) + 600 μL serum-free DMEM ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were diluted to 1 M urea with 50 mM ambic and digested with Trypsin (Promega #V5111) for 10 hours at 1:125 enzyme:protein at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... ribosome positioning was determined by primer extension/reverse transcription using AMV reverse transcriptase (1 U/μl) (Promega) in the presence of 125 μM of each of dTTP ...
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bioRxiv - Microbiology 2019Quote: ... The pellet was dissolved in 1% (w/v) RapiGest (Waters) (10 µL) and trypsin (Sequencing grade, Promega) solution was added (90 µL ...
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bioRxiv - Molecular Biology 2021Quote: ... lysed in 1 × passive lysis buffer and analyzed using the Dual-Luciferase Reporter Assay System (Promega Corporation) and a SpectraMax L Microplate Reader (Molecular Devices ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated for 1 h with the secondary antibody anti-mouse IgG HRP conjugate (W4021, Promega, U.S.A.) diluted at 1/3000 in 10% Blocking One/TTBS ...
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bioRxiv - Microbiology 2020Quote: ... the blots were incubated for 1 hour with secondary antibody (goat anti-rabbit IgG-HRP, Promega W4018) that was diluted 1:10000 in blocking buffer ...
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bioRxiv - Microbiology 2019Quote: ... The reaction mixture (25 μL) used for cDNA synthesis contained 1⋅25 μM random hexamer primers (Promega), 250 μM of each deoxynucleoside triphosphate (dNTP) ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein digestion on beads was carried out using a ratio 1:50 sequencing grade modified trypsin (Promega): protein extract in 100 mM ammonium bicarbonate at 37 °C overnight ...
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bioRxiv - Plant Biology 2019Quote: ... using 1 - 2 µg of total RNA and 250 ng of a mixture of random hexanucleotides (Promega) and incubated for 50 min at 50°C ...
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bioRxiv - Immunology 2019Quote: ... In vitro pmel-1 T cell-mediated cytotoxicity assay with CytoTox-ONE Homogeneous Membrane Integrity Assay (Promega). protocols.io dx.doi.org/10.17504/protocols.io.6j4hcqw
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bioRxiv - Developmental Biology 2021Quote: ... the reaction mixtures for in vitro transcription were treated with RQ-1 RNase-free DNase I (Promega). Synthesized mRNAs were treated with phenol-chloroform to remove protein components ...
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bioRxiv - Biophysics 2021Quote: ... the cells were transfected with 1 – 2 μg EphA2-mTurquoise plasmid DNA using FuGene HD (Promega, #E2311) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... cells were transfected with 500 ng of either HDM-SARS-Spike-delta21 or HDM_Spike_RBD_B7-1 plasmids and 1.5 ng of Transfection Carrier DNA (Promega, E4881) using BioT reagent (Bioland Sci ...
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bioRxiv - Microbiology 2020Quote: ... RNA (1 μg) was converted to cDNA using the GoScript™ Reverse Transcription System (Promega, Madison, WI). The cDNA was synthesised was used for qPCR using the iQTM SYBR® Green Supermix (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2021Quote: ... Dilutions of antibodies applied were: rabbit anti-ferritin 1:10000 (primary) and anti-rabbit HRP conjugated (Promega) 1:10000 (secondary).
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bioRxiv - Microbiology 2021Quote: ... At 1 × s after injection of 20 µl of the substrate solution (Renilla Luciferase Assay System, Promega), relative light units (RLUs ...
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bioRxiv - Neuroscience 2020Quote: ... Tissues were then stained using the following primary antibodies overnight: mouse anti-β Galactosidase (1:1000; Promega), rabbit anti-Er81 (1:5000 ...
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bioRxiv - Cell Biology 2020Quote: 1 µg of total RNAs were reverse transcribed with Moloney murine leukemia virus Reverse Transcriptase (M170B, Promega). 100 ng cDNA and FastStart Universal SYBR Green Master (04913850001 ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were digested in-gel using trypsin (Gold Promega, 1 μg per band, overnight at 30°C,) as previously described (Thouvenot et al. ...
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bioRxiv - Microbiology 2022Quote: ... Larvae were immobilized in the dish using a drop of 1% low-melting-point agarose (V2111, Promega) solution in 80% glycerol ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were then incubated with 1:50 enzyme to protein ratio of sequencing-grade trypsin (Promega) overnight at 37 °C ...
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bioRxiv - Biophysics 2022Quote: ... The embryos were then incubated in a 1:5,000 dilution of TMR HaloTag Ligand (5 mM; Promega) in Danieau’s medium ...
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bioRxiv - Plant Biology 2022Quote: ... Protein expression was confirmed by Western blot with Anti-Halo Tag monoclonal antibody (1:2000) (G9211, Promega).
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bioRxiv - Biochemistry 2022Quote: ... the samples were incubated with trypsin (trypsin to substrate ratio of 1:50, Promega, cat. no. V5111) and 1 mM CaCl2 for 16 h at 37°C ...
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bioRxiv - Plant Biology 2021Quote: ... For multiple day measurements plants were sprayed daily with 1 mM D-luciferin (Promega, Fitchburg, Wl, USA) at 10 am ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were incubated with cell-permeant TMR red Halo-tag ligand (1:2,000 for 15 min, Promega) 24 hours post-transfection ...
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bioRxiv - Microbiology 2020Quote: ... and 1 μg of a plasmid expressing the VSV G glycoprotein using the FuGene HD reagent (Promega). Forty-eight hours later ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were then incubated with 1:50 enzyme to protein ratio of sequencing-grade trypsin (Promega) overnight at 37 °C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Bacterial DNA was extracted from 1 ml of culture using the Wizard genomic DNA purification kit (Promega). Evolved population DNAs were sequenced at the GeT-PlaGe core facility (https://get.genotoul.fr/) ...
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bioRxiv - Developmental Biology 2019Quote: ... Immuno-signals were visualized with an alkaline-phosphatase (AP)-conjugated anti-rabbit IgG antibody (1:5000, Promega). Western blot revelation was performed using a cheminoluminescence digital imaging system (ImageQuant Las-4000 Mini ...
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bioRxiv - Immunology 2019Quote: ... at 37ºC for 1 hr followed by treatment with 20 µg proteinase K (Promega, Madison, WI, USA) at 45ºC for 1 hr to remove protein ...
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bioRxiv - Developmental Biology 2019Quote: ... according to manufacturer’s instructions with the addition of 1 µl RNAse inhibitor (RNAsin Plus RNase Inhibitor, Promega).
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bioRxiv - Systems Biology 2019Quote: ... Proteins were digested on-filter (16 hr, 37 °C) with sequencing-grade modified trypsin (50:1, Promega). Proteolysis was terminated and peptides eluted with 20 μL of 10% trifluoroacetic acid followed by centrifugation (13,000 ×g ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were resuspended in 400μL FACS buffer (1.5% BSA, 2.5mM HEPES, 1mM EDTA in PBS) containing 2.5% RNAsin and 1/10000 DAPI (Promega). Cells for re-injection experiments were isolated as above and subjected to a further two centrifugation steps (1min at 200xg ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... at a ratio of 1:166 to Opti-MEM media in combination with extracellular NanoLuc Inhibitor (Promega) diluted 1:500 (10 μL [30 mM stock] per 5 mL Opti-MEM plus substrate) ...
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bioRxiv - Genomics 2021Quote: ... cells were transfected in a 3:1 ratio of FuGENE® HD Transfection Reagent (Promega cat # E2311) and plasmid DNA (pcDNA3.1-HMGB1P1-3xHA ...
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bioRxiv - Physiology 2020Quote: ... proteins were digested by incubation with sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin) for 12 h at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Nluc and BRET-FL signals were sequentially acquired by IV-injection with 1/40 diluted furimazine (Promega) in 100 μL sterile PBS and applying open (for Nluc ...
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bioRxiv - Microbiology 2021Quote: ... proteins were digested overnight with sequencing-grade porcine trypsin (1:25, w/w, Promega, Fitchburg, MA, USA). The resulting vacuum-dried peptides were resuspended in water containing 0.1% (v/v ...
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bioRxiv - Immunology 2019Quote: ... were transfected with indicated 1.33 μg HIV-1 env DNA and 2.66 μg Q23gΔenv using Fugene6 (Promega), harvested 48 hr post-transfection ...