Labshake search
Citations for Promega :
2351 - 2400 of 2907 citations for TREM 1 Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2021Quote: Pulldowns with cellular extracts were carried out as described for GFP-immunoprecipitations using 1-2 μg of GST-fused proteins bound to glutathione magnetic beads (Promega). For immunoprecipitations of recombinant proteins ...
-
bioRxiv - Plant Biology 2021Quote: ... 1 μg of RNA was converted into cDNA with M-MLV RNase H–Point Mutant reverse transcriptase (Promega Corp., USA) and an anchored oligo dT21 primer (Metabion ...
-
bioRxiv - Plant Biology 2020Quote: ... and then reverse-transcribed with a cox1 specific primer (Atcox1-1; table S1) using M-MLV (Moloney Murine Leukemia Virus) Reverse Transcriptase (RNase H minus, point mutant; Promega). The RNA template was degraded by adding 1/5 volume of 1 M NaOH to the reaction mixture and incubating at room temperature for 10 minutes ...
-
bioRxiv - Plant Biology 2020Quote: ... The soluble fraction was then digested for 20 h at 37°C using a 1:50 ratio of trypsin (Promega) to extracted protein while gently shaking ...
-
bioRxiv - Microbiology 2020Quote: Replication competent HIV-1 and VSV-G pseudotyped HIV-1 GFP vectors were produced by transfection of HEK293T cells in T150 flasks using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: A random-primers based reverse transcription of 1 μg of RNA was performed with the A3500 Reverse Transcription System Kit (Promega), followed by quantitative real-time PCR on cDNA using the FastStart Essential DNA Green Master kit (Roche ...
-
bioRxiv - Plant Biology 2020Quote: ... Proteins were extracted from protoplasts after a 16h incubation in WI medium using 100 μl of 1 × passive lysis buffer (Promega). Luciferase activities were measured using the Dual Luciferase Reporter Assay kit (Promega ...
-
bioRxiv - Microbiology 2020Quote: ... and LgBiT Protein (1:100)/HiBiT Lytic Substrate (1:50) in Nano-Glo HiBiT Lytic Buffer (25 μl) (Nano-Glo HiBiT Lytic Detection System; Promega) were mixed and incubated for ten minutes at room temperature according to the modified manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2020Quote: ... 1 μg of DNase treated RNA was used for cDNA synthesis by oligo-dT primers and MLV reverse transcriptase (Promega) enzyme ...
-
bioRxiv - Microbiology 2021Quote: ... and LgBiT Protein (1:100)/HiBiT Lytic Substrate (1:50) in Nano-Glo HiBiT Lytic Buffer (25 μl) (Nano-Glo HiBiT Lytic Detection System; Promega) were mixed and incubated for ten minutes at room temperature according to a modified version of the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2020Quote: ... Quantitative real time RT-PCR (qPCR) was performed using gene-specific primers (Supplemental Table 1) and the fluorescent dye SYBR Green (Promega). All quantifications were normalized to the TIP41 (AT4G34270 ...
-
bioRxiv - Microbiology 2021Quote: ... A series of concentrations from 50-600nM in symmetric and asymmetric forward and reverse primer concentrations were added to the RT-qPCR reaction using the GoTaq 1-Step RT-qPCR System (Promega) according to the manufacturer’s instructions using the standard annealing temperature of 60°C ...
-
bioRxiv - Genomics 2021Quote: ... Inserts were cut out with appropriate restriction enzymes (Suppl. Table 1) and ligated with pre-digested pGL4.23[luc2/minP] (Promega, # E8411) plasmid upstream of a minimal promoter and a firefly luciferase gene ...
-
bioRxiv - Microbiology 2021Quote: ... A 10µl of sample was mixed with a 50µl portion of GAR-1 reagent (targeting Systems) and then measured in the luminometer (Promega, USA)36.
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... To the first pool 1 µl/mL DMEM/F12 (no phenol red) of HaloTag® NanoBRET™ 618 Ligand (Promega) was added and to the second pool DMSO ...
-
bioRxiv - Systems Biology 2020Quote: ... pH 8.0 and digested for 16 hours on a shaker at 37 °C with a 1:40 ratio of Trypsin/Lys-C mix (Promega). Each sample was de-salted using HLB plates (Oasis HLB 30μm ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1 ug (for cellular RNA) and 20 ng (for EV RNA) was treated with 1 U of DNAse I RNAse-free (#EN0521 PROMEGA) according to manufacturer instructions ...
-
bioRxiv - Microbiology 2019Quote: HIV-1 and lentiviral particles were produced by transfection of HEK293T cells in T150 flasks using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... and the pSARP-12R4-9 input library with a 3:1 transfection reagent:DNA ratio using Fugene 6 transfection reagent (Promega #E269A). At 72 hrs ...
-
bioRxiv - Molecular Biology 2021Quote: ... RNA pellets were resuspended in 8µl of DEPC water and incubated with µl (1U DNAse) and 1 µl of 10X DNAse buffer (Ref. M6101, Promega) for 30 min at 37°C ...
-
bioRxiv - Microbiology 2019Quote: ... Cross-links of input and IP material were reversed by adding 10 μl 5 M NaCl and 4 μl 10 mg ml−1 Proteinase K (Promega), and incubated for 4 hours at 65°C ...
-
bioRxiv - Microbiology 2020Quote: ... This was followed by in-gel digestion using 1:100 ratio enzyme:sample (w:w) using as enzyme sequencing grade modified trypsin (Promega, Madison, WI) overnight at 37°C ...
-
bioRxiv - Cancer Biology 2020Quote: Lentiviral and retroviral particles were produced by co-transfecting HEK293T cells with viral vectors and packaging plasmids at a mass ratio of 1:0.75:0.3 using FuGENE®6 (Promega, E2691). Virus-containing medium was filtrated (0.45 μm ...
-
bioRxiv - Cell Biology 2021Quote: ... Washed beads were re- suspended in 150 µl digestion buffer and incubated for 4 hours with 1 µg trypsin (Promega) at 37 °C ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were again washed with DPBS and incubated with 90 μL of a 1/1000 dilution of furimazine stock solution (Promega) in 0.1 % BSA/HBSS ...
-
bioRxiv - Cell Biology 2020Quote: ... and digested overnight at 37°C with 1:50 enzyme:substrate ratio (wt/wt) of sequencing grade trypsin (Promega, Madison, WI) as described 59 ...
-
bioRxiv - Genomics 2021Quote: ... Cell number was normalized before seeding by measuring ATP levels in a 1:2 dilution series of digested organoids with CellTiter-Glo (Promega). The number of cells matching 10,000 photons (Berthold Technologies ...
-
bioRxiv - Biophysics 2020Quote: ... for 2 hours at 37° C and subsequently diluting to 1 M urea with 50 mM NH4HCO3 and finally adding 2% (w/w) trypsin (Promega) for 14 hours at 37° C ...
-
bioRxiv - Biophysics 2020Quote: ... as well as expression construct of interest in a 9:8:1 mass ratio into HEK293T cells using FuGENE HD Transfection Reagent (Promega) per manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were resuspended at 2 x 106 cell mL-1 in complete media and HaloTag substrate conjugated to TMR (Promega) was added at a final concentration of 5 μM ...
-
bioRxiv - Cell Biology 2020Quote: ... Membranes were washed 3 times for 15 min in TBST and subsequently incubated with species-specific HRP-conjugated secondary antibodies (1:10000; Promega) in 3% BSA-TBST for 1h at room temp ...
-
bioRxiv - Cell Biology 2021Quote: ... for 1 hour at 37°C and DNA was extracted using the Wizard SV gel and PCR cleanup system (Promega). Input and immunoprecipitated DNA was subjected to quantitative PCR using the oligonucleotides shown in Table 1 ...
-
bioRxiv - Cancer Biology 2021Quote: ... The desired PCR fragments were isolated from a 1% agarose gel and purified using the Wizard Genomic DNA Purification Kit (Promega). In order to identify the specific edition ...
-
bioRxiv - Biophysics 2022Quote: ... 60 µm wide (diameter at 1/e2 peak intensity) in the sample plane as characterised using a sample of immobilised standard fluorescent microbeads (Promega). The source power was 30 mW ...
-
bioRxiv - Molecular Biology 2022Quote: ... The SELENOP 3’UTR and the control plasmid were linearized using Bsb 1 and in vitro transcribed using the Ribomax kit (Promega). The RNA was then purified using p30 size exclusion columns (Bio-Rad ...
-
bioRxiv - Biochemistry 2022Quote: ... Samples were diluted with ddH2O 1:5 and proteins were digested for 20 h at 37°C using trypsin (Trypsin Gold, Promega) at an enzyme/protein ratio of 1:50 ...
-
bioRxiv - Biochemistry 2022Quote: ... proteins were washed three times with 150 μL S-Trap binding buffer and subsequently digested by addition of digestion buffer (500 mM ABC) containing 1:25 (w/w) trypsin (Trypsin Gold, Promega) and incubation at 37°C for three hours ...
-
bioRxiv - Genomics 2022Quote: ... The p-dCas9-KRAB and p-PB-Transposase plasmids were co-transfected into NCCIT cells (ATCC) at 70% confluency using a 6:1 ratio of Fugene HD (Promega) for 48 hours ...
-
bioRxiv - Genomics 2022Quote: ... Then the gel beads were washed with DPBS three times and 200 μL of proteinase K solution (1 mg/mL proteinase K [Promega, Madison ...
-
bioRxiv - Molecular Biology 2022Quote: ... samples were dissolved in 50 μL of 50mM TEAB and treated with 1 μL of recombinant PNGaseF (Promega, Madison, WI) overnight at 37 °C ...
-
bioRxiv - Molecular Biology 2022Quote: ... DNA was eluted from the beads using the elution buffer (1% SDS and 100 mM NaHCO3) and purified by PCR purification kit (Promega). The final products were analyzed by quantitative real-time PCR with the primers listed below:
-
bioRxiv - Microbiology 2022Quote: ... Both cassettes were built by first cloning the wild-type locus and 1 kb flanking region in the pGEM-T plasmid (Promega) and subsequently modifying the plasmid to add the HA-tag coding sequence using the Q5 Site-Directed Mutagenesis kit (NEB ...
-
bioRxiv - Biochemistry 2022Quote: ... The gels were dehydrated with acetonitrile and rehydrated with 10 mM ammonium bicarbonate containing 13 ng µl-1 trypsin (Promega). The protease digestion was carried out for 12 h at 37 °C following which the peptides were extracted from the gels using 1:2 (v/v ...
-
bioRxiv - Microbiology 2022Quote: ... the HSV-1 ICP0 3’UTR was amplified from pICP0(HSV-1) using psiV1ICP0UTRf and psiV1ICP0UTRr primers and inserted into psiCHECK-2 (Promega). psiChHVICP03UTR was constructed by inserting the synthesized ChHV ICP0 3’ UTR into the same position ...
-
bioRxiv - Cell Biology 2022Quote: ... for 2 h followed by a second digest with trypsin at 35°C overnight at 1200 rpm (1:50 ratio w/w, Promega). Proteolysis was stopped by the addition of 1% (v/v ...
-
bioRxiv - Molecular Biology 2022Quote: ... the concentrated sample was removed from the ultra-centrifugal filter and a 1:50 ratio of mass spectrometry grade Trypsin Gold (Promega) added to the sample which was incubated with shaking for > 12 hours at 37 °C in a thermal heat block ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... the proteins were alkylated with iodoacetamide (100 mM for 1 h at room temperature in a dark chamber) and digested with trypsin (Promega) diluted in ammonium bicarbonate (0.1 M for 24 h at 37 °C) ...
-
bioRxiv - Cancer Biology 2022Quote: ... The lysate was washed in Nuclei wash/resuspension buffer (1xPBS completed with 1% BSA and 0.2U/ul RNAsin Plus (Promega, #N2611) and passed through a 40 μm cell strainer ...
-
bioRxiv - Cell Biology 2022Quote: ... synthesis was done by using 1 μg RNA as template and the Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase (Promega), in 20 μl reaction volume according to the instructions manual ...
-
bioRxiv - Cell Biology 2022Quote: ... and dried in at 37 °C before overnight digestion with 30 μL trypsin (1:100 g/g, sequencing grade modified trypsin V5111, Promega) at 37 °C ...