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Citations for Promega :
2251 - 2300 of 5637 citations for hsa mir 185 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and the PCR products of 5’ RACE were cloned using pGEM®-T Easy Vector Systems (Promega, WI, USA). Purified PCR fragments and pGEM cloning 5’-terminal sequence of BToV were sequenced using primers designed for every ∼300 bp of the BToV genome ...
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bioRxiv - Microbiology 2020Quote: Illumina P5 and P7-barcoded adaptors were added by PCR on gDNA samples according to the GoTaq protocol (Promega). PCR amplicons were gel purified and sequenced on a HiSeq4000 (Illumina ...
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bioRxiv - Bioengineering 2020Quote: ... a 632 bp fragment of the 121-promoter [15] was amplified by PCR (Suppl. Table 1) and inserted between the BglII and HindIII sites of pGL4.10 (Promega) using NEBuilder HiFi DNA Assembly Master Mix.
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bioRxiv - Microbiology 2022Quote: ... The PCR product was cloned into a T Easy plasmid using the pGEM®-T Easy Vector (Promega, USA), following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... The 1400 bp long DNA fragments were purified (Wizard®SV Gel and PCR Clean-Up System, Promega, Germany) and sequenced using the same 27f and 1492r primer pair ...
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bioRxiv - Epidemiology 2019Quote: ... The 16S rDNA touchdown PCR was performed in 20 µL reactions containing 1× GoTaq® Green Mastermix (Promega, USA), 0.4 µM of each primer SpirRNAF and SpirRNAR (S2 Table ...
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bioRxiv - Plant Biology 2020Quote: ... The PCR product was subjected to adenine overhanging and inserted into pGEM T-easy vector (Promega, Madison, WI, USA) for sequencing ...
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bioRxiv - Biochemistry 2020Quote: ... pKS was nicked with Nt.BbvCI and purified using Wizard SV Gel and PCR Clean-Up System (Promega, Cat#A9281). The resulting short ssDNA fragment was then replaced with a 5-fluoro-2’-deoxycytidine (FdC)-modified oligonucleotide (400 nM ...
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bioRxiv - Neuroscience 2019Quote: ... and mouse Cbln2 E2 fragments were PCR-amplified from genomic DNA of individual species and inserted into pGL4.24 vector (Promega). The sequences of the PCR primers and synthetic oligonucleotides are listed in Supplementary Table 1.
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bioRxiv - Molecular Biology 2020Quote: ... The ssDNA product was used as a template for 25 cycles of PCR using a 50µl GoTaq reaction (Promega).
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bioRxiv - Genetics 2019Quote: ... genes were isolated from C57BL/6 mouse genomic DNA by standard PCR and inserted into the pGL4.10 vector (Promega).
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bioRxiv - Molecular Biology 2019Quote: ... The two fragments were then ligated using an overlap extension PCR reaction and ligated into pGEM/T-Easy (Promega). Positive clones were digested with AscI and SalI and the dropout was subsequently ligated into an AscI- and SalI-linearized pENTR-PpU6-sgRNA-NGG plasmid.
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bioRxiv - Bioengineering 2019Quote: ... Ligations of the digested plasmid backbones and PCR products occurred overnight at 16°C using T4 DNA ligase (Promega) prior to transformation into chemically competent Novablue E ...
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bioRxiv - Molecular Biology 2020Quote: Outward facing PCR reactions were performed on 6ng of random primed cDNA using the GoTaq G2 DNA polymerase (Promega) following the manufacturer’s recommendations in the Techne TC-3000 PCR thermal cycler (initial denaturation of 2 min 95 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... STR profiling was carried out by PCR amplification of nine STR loci plus amelogenin (GenePrint 10 System; Promega, #B9510), fragment analysis (3730XL DNA Analyzer ...
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bioRxiv - Pathology 2021Quote: ... The DNA concentration of each PCR product was determined using a QuantiFluor™-ST fluorescent quantitative system (Promega, USA), and mixed with the appropriate proportion based on sequencing requirements ...
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bioRxiv - Microbiology 2020Quote: The PCR conditions for 50 μL were 25 μl of GoTaq G2 Hot Start Green Master Mix (#M7423, Promega), 0.5 μM of each primer (Supplementary Table 3 ...
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bioRxiv - Microbiology 2020Quote: ... This amplified PCR product was then cloned into the pGEM-T Easy Vector using T4 ligase (Promega, Madison, WI), resulting in the construction of pCjNC110::cat which was then transformed into chemically competent E ...
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bioRxiv - Microbiology 2021Quote: ... Marker genes in isolated DNA were PCR-amplified using GoTaq Master Mix (Cat. No. M5133, Promega, Madison, WI, USA) as described in Caporaso et al.[24] PCR products were cleaned and normalized using the SequalPrepTM Normalization Plate Kit (Cat ...
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bioRxiv - Molecular Biology 2022Quote: ... The library was amplified for six PCR cycles with short primers followed by a ProNex size selection step (Promega) in a 1:2.95 (v/v ...
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bioRxiv - Molecular Biology 2022Quote: ... Semiquantitative PCR reactions were carried out with 1–2 μL of cDNA and 0.2 μL of GoTaq DNA Polymerase (Promega) in a total reaction volume of 30 μL according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Regions of interest were amplified in a PCR reaction with GoTaq G2 Flexi DNA polymerase (Promega, Madison, WI, USA), and PCR-amplified samples were run on a 1,2% TBE-agarose gel for size separation ...
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bioRxiv - Cancer Biology 2022Quote: ... a 3kb region of the promoter containing HIC1 binding sites was amplified by PCR from human genomic DNA and cloned into the pGl4.26-luc vector (Promega); primers are listed in supplementary Table S1 ...
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bioRxiv - Synthetic Biology 2022Quote: ... The presence and orientation of DNA fragments were confirmed by colony PCR using GoTaq Green Master Mix (Promega, USA), whereas the sequence of the genetic construct was checked by Sanger sequencing reactions (Macrogen Inc. ...
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bioRxiv - Neuroscience 2022Quote: Riboprobes for in situ hybridisation were generated by cloning transcript-specific PCR products into pGEM-T-Easy (Promega, A1360) using the TA-cloning method ...
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bioRxiv - Genetics 2022Quote: ... The corresponding bands were cut out and purified using wizard SV gel and PCR clean-up system (Promega, A9282). The concentrations of purified PCR products were measured by Nanodrop 2000c and then diluted into 70-100ng/uL with distilled water accordingly ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR fragments with expected size were purified and TA cloned in the pGEM-T Easy Vector (Promega, Madison, WI) for DNA sequencing ...
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bioRxiv - Molecular Biology 2022Quote: ... individual target sequences were PCR amplified and the amplicons were sub-cloned into separate pGEM-T Easy vector (Promega) at TA cloning site ...
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bioRxiv - Developmental Biology 2022Quote: ... These PCR products were subcloned downstream of the luciferase gene contained in the pGL3-Basic commercial vector (Promega – E1751). Mutation of the highly cross-species conserved miR-483-3p seed target sequence from AGGAGUG to AGGAACG (mouse ...
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bioRxiv - Molecular Biology 2024Quote: ... The PCR product was purified after migration on a 1% agarose TAE 0.5X gel using the Wizard PCR Clean-Up system (Promega) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product was digested with ApaI and XhoI and then purified (Wizard® SV Gel, Promega, WI, USA). The eluted fragment was ligated downstream of an inducible acetoacetate decarboxylase promoter (Padc ...
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bioRxiv - Neuroscience 2023Quote: ... DNA were purified using the Wizard® SV Gel and PCR Clean-Up System protocol (Promega, Madison, Wisconsin, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Purification and assembly of DNA fragments were performed using a Wizard SV Gel and PCR Clean-up System (Promega) and a NEBuilder HiFi DNA assembly cloning kit (New England Biolabs) ...
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bioRxiv - Microbiology 2023Quote: ... The FAM-labeled probes were purified using the Wizard® SV Gel and PCR Clean-Up System (Promega, USA) and quantified with a NanoDrop 2000C (Thermo ...
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bioRxiv - Microbiology 2023Quote: ... Each PCR product was digested with XhoI and BamHI and ligated with the T4 DNA ligase (Promega, Cat #: M1794) into a XhoI and BamHI pre-digested pcDNA3.1-mCherry-HIS vector ...
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bioRxiv - Molecular Biology 2023Quote: ... 1/20th of the resulting cDNA was used then analyzed by qRT-PCR using GoTaq qPCR master mix (Promega). The following primer pairs were used:
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bioRxiv - Molecular Biology 2023Quote: The human MEOX1 enhancer syntenic to the mouse Meox1 peak9/10 enhancer3 fragment along with the mouse Il1b peak5 and peak6 fragments were PCR amplified and cloned into the XhoI and HindIII sites in the Firefly luciferase plasmid pGL4.23 (Promega) using the Cold Fusion kit (System Biosciences Inc ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μl cDNA of WT and CRISPR cln3 mutant lines was used as PCR template and amplicons were cloned into the pGEMT vector following the manufacturer’s instructions (Promega). Selected clones were sent for sequencing.
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bioRxiv - Evolutionary Biology 2023Quote: ... Denaturated RNA (5μg) was retrotranscribed and used for quantitative PCR using GoTaq qPCR Master Mix according to the manufacturer’s instructions (Promega) at a dilution of 1/10 for mycelium and 1/7 for rice leaves ...
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bioRxiv - Cell Biology 2023Quote: ... Purified PCR products were in vitro transcribed using the T7 RiboMAX Express Large Scale RNA Production System (Promega, P1320). The transcripts were purified using the RNeasy Micro kit (QIAGEN ...
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bioRxiv - Microbiology 2023Quote: ... USA) was performed using primer 27F on a V1-V3 16S rRNA gene colony-PCR amplicon (GoTaq Green, Promega) of primers 27F and 519R ...
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bioRxiv - Microbiology 2023Quote: Approximately 100 ng of full-length PCR product was ligated into the pGEM-T Vector System (Promega, Madison, WI), at a 3:1 molar ratio of insert ...
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bioRxiv - Neuroscience 2023Quote: Adult worms were lysed following standard protocols and PCRs were performed using GoTaq Green Master Mix (Promega, REF-M7123). Mutant alleles were distinguished from wild-type by imaging Restriction Fragment Length Polymorphisms (RFLP ...
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bioRxiv - Pathology 2023Quote: ... PCRs were performed in a final volume of 20 µL containing 1X of Colorless GoTaq® Flexi Buffer (Promega), 0.0625 mM of each dNTP ...
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bioRxiv - Plant Biology 2023Quote: ... The size of the amplified PCR products was ascertained by utilizing a 100 bp GeneRulers™ DNA ladder (Promega). PCR products were sent to Genewiz/ Azenta company for DNA sequencing in both directions.
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bioRxiv - Neuroscience 2023Quote: ... For all animals universal PCR reaction was used as follows: 12.5 μl of GoTaq Hot Start Green Master Mix (Promega), 0.5 μl of each primer (25 μM ...
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bioRxiv - Plant Biology 2023Quote: ... Gene-specific and transposon multiplex primer PCR was performed under standard conditions with 2X GoTaq Green Master Mix (Promega) with 5% DMSO (v/v).
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bioRxiv - Cancer Biology 2023Quote: IDH1 and NNT promoter sequences were amplified from genomic DNA of human primary melanocytes by PCR and cloned into pGL4.12 (Promega). Mutagenesis of E-boxes was performed using a QuikChange Site-Directed Mutagenesis Kit (Stratagene) ...
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bioRxiv - Genomics 2023Quote: ... Reference allele enhancer sequences for hZRS and hs737 were obtained via PCR cloning from human genomic DNA (Promega, G304A). Primers used for each enhancer sequence are outlined in Table S2 ...
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bioRxiv - Neuroscience 2023Quote: ... The expression of reporter and reference genes were analyzed by quantitative PCR using GoTaq-qPCR Master Mix (Promega, #A6002), with the real-time PCR system (Light Cycler 480 ...