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Citations for Promega :
2251 - 2300 of 5345 citations for 6H Imidazo 4 5 1 de acridin 6 one 5 3 diethylamino propyl amino 8 hydroxy since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... the cleaned PCR product was eluted from the beads in 10 µL of buffer solution containing 10 mM Tris-HCl pH 8.0 with 50 mM NaCl and the DNA concentration was quantified on a Quantus Fluorometer using Quantifluor ONE dsDNA dye (Promega #E4891).
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bioRxiv - Bioengineering 2023Quote: ... cells were transfected at > 80% confluence with all-in-one PiggyBac plasmids containing different Cas13d orthologs using FuGENE® HD Transfection Reagent (E2311, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... low passage cells were plated at a density of 15,000 cells per well in a 96-well plate and transfected at > 80% confluence with all-in-one PiggyBac plasmids containing CasRx or DjCas13d using ViaFect™ Transfection Reagent (E4981, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... cells were transfected at > 80% confluence with all-in-one PiggyBac plasmids containing different Cas13d orthologs using FuGENE® HD Transfection Reagent (E2311, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... low passage cells were plated at a density of 15,000 cells per well in a 96-well plate and transfected at > 80% confluence with all-in-one PiggyBac plasmids containing CasRx or DjCas13d using FuGENE® HD Transfection Reagent (E2311, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10 μL of the dilution was mixed with 20 μL of passive buffer which was then mixed with 30 μL ONE-Glo™ EX Luciferase Assay Reagent (Promega) and incubated at room temperature for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: ... cell viability was assessed using the MTS assay (CellTiter 96® AQueous One Solution Cell Proliferation, G3580, Promega, Madison, WI, USA). In brief ...
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bioRxiv - Genomics 2023Quote: ... TRs were amplified using the forward and reverse primers plus an M13 primer with one of the four fluorophores with GoTaq polymerase (Promega #PRM7123) using PCR program ...
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bioRxiv - Biophysics 2023Quote: To investigate CBD influence on the cells viability and metabolic activity the cells were tested using CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega) with tetrazolium compound ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell viability was measured 72 hr after drug treatment using the CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: Cytotoxic activity of the CAR T cells was evaluated with the Promega ONE-Glo™Luciferase Assay (Promega Corporation; cat# E6120) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The MTS assay was carried out at certain time points using The CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega #G3580) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... cells were gently washed once with 1X DPBS and MTS reagent of 20 μL (Cell Titer-96 Aqueous One Solution, Promega, USA) in 80 μL of cell culture media for 3 h (37°C and 5% CO2) ...
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bioRxiv - Molecular Biology 2020Quote: ... Plasmid transfection of U2OS cells with PIF1 variants was carried out using FuGENE 6 Transfection Reagent (Promega) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2021Quote: HeLa cells were plated at 100,000cells in 2 ml media onto 35mm MatTek glass bottom dishes and transfected with 0.9µg RUSH plasmid and 0.1µg eGFP-GalT or LAMP1-GFP using 100µl Opti-MEM and 3µl Fugene 6 transfection reagent (Promega) one day before imaging ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were washed again and 6 µg of plasmid DNA was added with FugeneHD transfection reagent (Promega). Supernatants were collected at 24 ...
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bioRxiv - Developmental Biology 2020Quote: ... 6 uL of RNA was used for each cDNA synthesis (GoScript™ Reverse Transcription System kit, Promega). qPCR was performed using Fast SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... following manufacturer’s guidelines and a ratio of 0.5 µg of plasmid:1.5 µL FuGene 6 (Promega, E2691) in Opti-MEM (Gibco) ...
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bioRxiv - Biochemistry 2020Quote: ... HeLa cells were transiently transfected with the PX459–sgRNF213-exon3 vector using FuGENE 6 Transfection Reagent (Promega). Forty-eight hours post-transfection ...
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bioRxiv - Cell Biology 2021Quote: ... Transfection was done on pre-seeded HeLa cells (70% confluency) with the FuGENE 6 Transfection Reagent (Promega) following standard protocol ...
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bioRxiv - Cell Biology 2022Quote: eGFP-HSP27 immunoprecipitations were performed in p62KO HeLa cells 24hrs following transient transfection using Fugene 6 (Promega). HeLa cells were treated with 750µM LLOMe for 2hrs prior to immunoprecipitation ...
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bioRxiv - Neuroscience 2022Quote: ... mouse Piezo2 (Lewis et al., 2017): 2.5 µg + 0.5 µg YFP) with Fugene 6 (Promega, Madison, WI) 48 hours prior to recording at a transfection ratio of 10 µL Fugene6:3 µg total DNA ...
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bioRxiv - Biochemistry 2023Quote: ... 2-4μg bacmid was transfected into Sf9 cells in 6 well plates using Insect-XPRESS (Promega E2311). Cells were incubated at 2°C for 5 days and bacmid YFP expression assessed by Leica DM IL LED Fluo microscope using a green fluorescent protein filter cube ...
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bioRxiv - Microbiology 2023Quote: ... and 0.25 μg vesicular stomatitis virus G glycoprotein expression plasmids using the Fugene 6 transfection reagent (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2022Quote: ... in 6-well plates with pActin-Gal4 and pUAS-Lgr4 vector using Fugene-HD (Cat. # E2311, Promega). Twenty-four hours after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... and then they were transfected with the expression plasmid DNAs by using FuGENE 6 (Promega, Madison, WI).
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bioRxiv - Cancer Biology 2023Quote: ... into HEK293T cells in 57 µL Opti-MEM and 6 µL FuGENE HD (Promega, Fitchburg, WI, USA) transfection reagent as described (Gerstmeier et al ...
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bioRxiv - Systems Biology 2024Quote: ... FuGene 6 (cat#: E2692) transfection reagent and sequencing grade modified trypsin (cat#: V5111) were purchased from Promega. Cas9 endonuclease was purchased from Integrated DNA Technologies ...
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A CRISPR activation screen identifies FBXO22 as an E3 ligase supporting targeted protein degradationbioRxiv - Biochemistry 2023Quote: ... FuGene 6 (cat#: E2692) transfection reagent and sequencing grade modified trypsin (cat#: V5111) were purchased from Promega. Cas9 endonuclease was purchased from Integrated DNA Technologies.
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bioRxiv - Cell Biology 2024Quote: ... Transfection with FCHSD2 constructs and RAB5 Q79L constructs was achieved using the FuGENE 6 (Promega, Madison, WI) transfection reagents and protocol ...
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bioRxiv - Immunology 2024Quote: ... PCR was done on gDNA from at least 6 individual larvae using GoTaq Green Master Mix (Promega) (Supp Fig 1A ...
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bioRxiv - Plant Biology 2020Quote: ... following the manufacturer’s instructions (eluted into 8 μl of TLE) before A-tailing in a reaction containing 2 μl of GoTaq buffer (Promega), 0.2 μl 10 mM ATP ...
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bioRxiv - Microbiology 2020Quote: ... post-transfection with or without OSM (10 ng/mL for 8 h) or IFN-α (1,000 U/ml for 16 hours) before lysis using Passive Lysis Buffer (Promega). Firefly and Renilla luciferase activity was then determined in a dual luciferase assay ...
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bioRxiv - Cell Biology 2022Quote: ... The bound proteins were digested by resuspending the beads in 200 μL freshly prepared digestion buffer (2M Urea, 100 mM Tris-HCl pH 8, 100 mM DTT) with 0.5 ug trypsin (Promega, V5111) and overnight incubation at room temperature while shaking ...
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bioRxiv - Immunology 2019Quote: 8 nM cap0 or 20 ng/μL VPg-linked RNA was translated using the Flexi Rabbit Reticulocyte Lysate system (Promega) in the presence or absence of 1.5 μM Ifit proteins ...
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bioRxiv - Molecular Biology 2020Quote: ... RL and FL activities were measured on 96-well plates using 2 µl of cell extract containing 8 µl of 1x PLB (Promega) and the dual-luciferase assay kit (Promega) ...
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bioRxiv - Systems Biology 2021Quote: ... In 18 hours cells were stimulated for 8 hours with a corresponding inducer and harvested in the passive lysis buffer (Promega). Luminescence of Firefly and Renilla luciferases was measured using dual-luciferase-reporter assay (Promega ...
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bioRxiv - Immunology 2019Quote: ... Samples were diluted 10-fold with 50 mM Triethylammonium bicarbonate buffer at pH 8 and incubated overnight at 37°C with sequencing grade trypsin (Promega) added at a 1:50 enzyme:substrate ratio (wt/wt).
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bioRxiv - Cell Biology 2019Quote: ... Samples were diluted 10-fold with 50 mM triethylammonium bicarbonate buffer at pH 8 and incubated overnight at 37°C with sequencing grade trypsin (Promega) at a 1:50 enzyme:substrate ratio (wt/wt).
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bioRxiv - Cancer Biology 2021Quote: ... Cell lysates were prepared by addition of 0.3N hydrochloric acid then 450nM Tris (pH=8) and metabolites were analyzed using the Glutamine/Glutamate Glo Assay (Promega; J8021) per manufacturer’s instruction.
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bioRxiv - Molecular Biology 2021Quote: ... the coding sequence of REST/NRSF ZF1-8 were amplified by PCR using cDNA as templates and cloned into the pTNT vector (Promega) between the EcoRI and XbaI sites ...
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bioRxiv - Developmental Biology 2022Quote: ... Luciferase assays were conducted 8 hours after stimulation using a dual luciferase kit (Dual-Luciferase® Reporter Assay System, Promega) according to manufacturer’s protocol.
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bioRxiv - Neuroscience 2022Quote: ... the absorbance was measured at 450 nm (CCK-8) or 560 nm (MTT) using a microplate reader (GloMax explorer multimode, Promega).
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bioRxiv - Biochemistry 2022Quote: ... Luciferase expression was measured at 8 hr (for translation) and 72 hr (for replication) using the Renilla Luciferase Assay System (Promega). Renilla luciferase luminescence was measured using a Clariostar Microplate Reader (BMG Labtech ...
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bioRxiv - Cell Biology 2022Quote: ... and the -1680 bp to +8 bp and -1525/-1495 element mutant were constructed in the pGL3-Basic luciferase reporter vector (Promega) using standard molecular biology techniques ...
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bioRxiv - Neuroscience 2022Quote: ... Digestion of proteins in the gel pieces was done by covering them with a trypsin solution containing (8 ng/µL sequencing-grade trypsin (Promega), dissolved in 50 mM NH4HCO3with 10% ACN) ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCR Tag analysis was performed either by adding 1.8 μL of yeast lysate used as template DNA to 6.25 μL of 2X GoTaq® Green Master Mix (Promega), 4.75 μL of nuclease-free water and 400 nM of each primer or by adding 1 µl of yeast lysate to 5 µl of DreamTaq Green PCR Master Mix (2X ...
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bioRxiv - Immunology 2022Quote: ... Ectopic Flag-tagged Galectin 8 or HA-tagged LILRB4 plasmid were transfected into the Mc38/B16/HEK294 cells with Fugene (Promega). A blank vector control was applied ...
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bioRxiv - Cancer Biology 2022Quote: ... CaspaseGlo assays were performed in white-walled 96-well plates to measure the activity of caspase 9 (cat. G8210) or caspase 8 (cat. G8200) (both from Promega) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... 8 μl of the terminated reaction mixture were then applied to a streptavidin biotin capture membrane (SAM2, Promega, Cat.# V2861) and further treated following the manufacturer’s recommendations ...