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Citations for Promega :
2201 - 2250 of 5396 citations for Rat Protein Glutamine Gamma Glutamyltransferase 2 TGM2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... and with fluorescence-based method using QuantiFluor Dye kit (Promega) and Quantus (Promega ...
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bioRxiv - Biochemistry 2023Quote: ... The Dual-Luciferase Reporter Assay System kit was from Promega. Coelenterazine 400a was from Nanolight Technology (Cat# 340).
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bioRxiv - Plant Biology 2023Quote: ... The Dual-Luciferase Reporter Assay System kit (Cat#E2940, Promega) was used to quantify fluorescence signals ...
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bioRxiv - Molecular Biology 2023Quote: ... ATP hydrolysis was then measured using ADP-Glo kit (Promega) by adding 5uL ADP-Glo reagent for 40 minutes followed by 10uL Kinase Detection Reagent for 1hour ...
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bioRxiv - Microbiology 2023Quote: ... extracted using the Wizard® Genomic DNA Purification Kit (Promega) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... synthesized using an AMV Reverse Transcriptase kit (Promega, Madison, WI), were amplified using SYBRGreen Power UP Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... RiboMAX™ Large Scale SP6 RNA polymerase kit (Promega, #P1280) was used for in vitro transcription on XbaI linearized pCS2+ vectors ...
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bioRxiv - Microbiology 2024Quote: ... the BacTiter-Glo microbial cell viability assay kit (Promega, USA) was employed for this purpose ...
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bioRxiv - Cancer Biology 2024Quote: ... Luciferase assays wered performed using the Luciferase System Kit (Promega) and luminescence was measured ...
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bioRxiv - Cancer Biology 2024Quote: ... CellTiter 96 non-radioactive cell proliferation assay kit (Promega, G4000) was used to determine cell growth and the manufacturer’s instructions were followed ...
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bioRxiv - Neuroscience 2024Quote: ... Luminescence was measured using the Dual Glo Luciferase Kit (Promega) following the manufacturer’s protocol in an ELISA plate reader (Tecan ...
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bioRxiv - Neuroscience 2024Quote: ... using the CytoTox 96® Non-Radioactive assay kit (Promega), according to the manufactureŕs instructions ...
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bioRxiv - Biochemistry 2024Quote: ... the TNT® Quick Coupled Transcription/Translation Kit from Promega was used ...
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bioRxiv - Immunology 2024Quote: ... LDH release was assessed using the Cytotoxicity Detection Kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: The biomass recovered from isolate incubation in broth medium was subjected to DNA extraction using the commercial kit Wizard® Genomic DNA Purification Kit (Promega Corporation, Madison, WI, USA), following the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was then incubated at 37°C for overnight trypsin digestion (trypsin 1:50 protein w/w ratio; Promega, Madison, WI). The digestion reaction was quenched by adding formic acid (FA ...
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bioRxiv - Biochemistry 2020Quote: ... Trypsinisation was carried out by incubating the gel pieces overnight at 37 °C in 50 µl of trypsin (1: 25:: trypsin: protein, 20 µg/ml, sequencing grade trypsin V5111, Promega, Madison, WI) prepared in ammonium bicarbonate (50 mM) ...
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bioRxiv - Immunology 2021Quote: ... and bound proteins were subjected to on-column digestion using mass spec grade Trypsin/Lys-C Rapid digestion enzyme (Promega, Madison, WI) at 70 °C for 2 h ...
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bioRxiv - Cell Biology 2019Quote: Full-length BUB-1 and ULP-1 cDNAs were cloned into pF3 WG (BYDV) Flexi® Vectors and expressed using the TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega). BUB-1 reactions included 35[S]-labelled methionine to allow for further detection ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were first digested for 5 h at 37 °C with 500 ng rLys-C Mass Spec Grade (Promega, Madison, WI, USA) before being diluted 4-fold with 100 mM Tris HCl pH 8.5 to reach a concentration below 2 M urea ...
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bioRxiv - Microbiology 2019Quote: ... In-gel digestion of selected protein bands or spots was performed overnight at 37 °C by incubation with trypsin (Sequencing grade, Promega, Madison, USA). Afterwards peptides were extracted as previously described [24] and samples were vacuum-dried using CentriVap Vacuum Concentrator (Labconco) ...
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bioRxiv - Bioengineering 2021Quote: ... Cell lysates were pre-cleared of any non-specific streptavidin-binding proteins by incubating with 60 µL of MagneSphere paramagnetic particles (Promega, Madison, WI) for 30 min with rotation ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were digested with 1 μg lysyl endopeptidase (LysC) (FUJIFILM Wako Pure Chemical Corporation) and 1 μg trypsin (Promega, Tokyo, Japan) overnight at 37°C on a shaking incubator ...
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bioRxiv - Molecular Biology 2021Quote: ... IPs were performed by incubating lysates equivalent to 20 mg total protein supplemented with RNase inhibitor - RNasin (Promega, Germany, Cat. No. N2511) and 1 mM DTT with 80 μl of anti-GFP magnetic beads GFP-Trap (Chromotek ...
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bioRxiv - Systems Biology 2022Quote: ... 102.2 µL of 100 mM ABC was added and proteins were digested for 16 hours at 37°C using 5 µg of trypsin (dissolved in trypsin resuspension buffer, Promega, Walldorf, Germany). Tryptic digestion was stopped by addition of 2.5 µL 100% formic acid ...
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bioRxiv - Cell Biology 2020Quote: ... The medium was then replaced with fresh medium in the incubator and protein release was induced by biotin addition (100 µM final) and following Furmazin (Promega, 1/500) addition ...
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bioRxiv - Molecular Biology 2020Quote: ... The band was cut and washed before the in-gel digestion of the proteins overnight at 37°C with a solution of modified trypsin (sequence grade, Promega, Charbonnières, France). The resulting peptides were extracted from the gel by one round of incubation (15 min ...
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bioRxiv - Synthetic Biology 2020Quote: ... and the bound proteins were subjected to on-cartridge digestion with mass spec grade Trypsin/Lys-C Rapid digestion enzyme (Promega, Madison, WI) at 70°C for 2h ...
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bioRxiv - Developmental Biology 2019Quote: ... the expression of the fusion protein was initiated by washing out Dox and changing the medium into CDMi containing protected furimazine (Promega; 1 μM). After the NLuc signal was confirmed 5-8 hours later ...
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bioRxiv - Biochemistry 2019Quote: ... A total of 100 µg of each protein sample was digested with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, Wisconsin, USA) by using filter-aided sample preparation (FASP ...
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bioRxiv - Molecular Biology 2021Quote: ... and incubated overnight at 25 °C or endoproteinase GluC in a GluC/protein ratio of 1/20 (w/w) (Promega Cat. No.V1651) and incubated overnight at 37 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... protein in the gel pieces was digested using 20 ng/μl of Trypsin solution made in 25mM ammonium bicarbonate (Promega, Madison, WI) by incubating at 37°C overnight ...
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bioRxiv - Plant Biology 2022Quote: ... The pCMX-PL2-NterHA/MED14 vector was constructed as described above and expressed as HA-MED14 proteins using the TNT T7 Coupled Reticulocyte Lysate System (Promega, Madison, WI). HA-MED14 prey proteins were incubated with the affinity-purified GST-PIF4 or GST-HMR bait proteins immobilized on glutathione Sepharose 4B beads (GE Healthcare ...
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bioRxiv - Plant Biology 2022Quote: ... Protein complexes were then expressed in vitro using the TNT SP6 Coupled Wheat Germ Extract System (Promega, Madison, WI, USA, Cat #L4130) and purified using Magne HaloTag Beads (Cat #G7282) ...
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bioRxiv - Physiology 2022Quote: ... protein samples were digested by incubated overnight at 37°C with sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin). Samples were acidified using 5% TFA and peptides cleaned up using the Phoenix 96× kit (PreOmics ...
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bioRxiv - Neuroscience 2022Quote: ... Protein digestion was processed for overnight by adding LysC and trypsin in a 1:50 ratio (µg of the enzyme to µg of protein; Promega; Cat#: V5071)) at 37° C and 1400 rpm ...
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bioRxiv - Systems Biology 2022Quote: ... 102.2 µL of 100 mM TEAB was added and proteins were digested for 16 hours at 37°C using 5 µg of trypsin (dissolved in trypsin resuspension buffer, Promega, Walldorf, Germany). Tryptic digestion was stopped by addition of 2.5 µL 100% formic acid ...
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bioRxiv - Developmental Biology 2022Quote: ... the expression of the fusion protein was initiated by washing out Dox and changing the media into CDMi containing protected furimazine (Promega; 1 μM). After the NLuc signal was confirmed 5-8 hours later ...
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bioRxiv - Neuroscience 2024Quote: ... or shRNA constructs with viral enzymes and envelope proteins (pMDLg/RRE, pRSV-REV and pVSV-G) using FuGENE6 transfection reagent (Promega Cat# E2692). HEK293T media was replaced with Neurobasal media (Thermo Fisher Scientific Cat# 21103049 ...
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bioRxiv - Cancer Biology 2023Quote: T cell cytotoxicity induced by BiTEs and BiTE-sialidase fusion proteins was measured by lactate dehydrogenase (LDH) release using CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, G1780). 10000 tumor cells and 50000 hPBMCs per well in 100 μL media were exposed to different treatments and incubated in 96 well plates at 37 °C (unless different ratio was specified elsewhere) ...
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bioRxiv - Systems Biology 2023Quote: ... proteins were precipitated with isopropanol50 and digested overnight at 37°C with 1:50 enzyme : protein ratio by Trypsin/Lys-C Mix (Promega GmbH, Germany). Resulting peptides were desalted on MicroSpin column (The Nest Group ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein solution was spun onto an S-Trap device (Protifi, Farmingdale, NY) and digested by 750 ng trypsin (Promega, Madison, WI) in 100 mM TEAB buffer at 37°C overnight ...
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bioRxiv - Systems Biology 2023Quote: ... and the bound proteins were subjected to on-cartridge digestion with mass spec grade Trypsin/Lys-C Rapid digestion enzyme (Promega, Madison, WI) at 70°C for 2h ...
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bioRxiv - Cancer Biology 2023Quote: ... and the protein pellet was resuspended in 50 mM ammonium bicarbonate and digested with sequencing-grade modified trypsin (Promega, Madison, WI, USA) overnight at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... the heated plates were allowed to cool down on ice and proteins digested by adding 1µl of 10ng Trypsin Gold (Promega, catalog number V5280) in 100mM TEAB and incubating at 37°C overnight ...
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bioRxiv - Biochemistry 2024Quote: Purified polar flagellin proteins at a concentration of 0.5 µg/µL in 25 mM ammonium bicarbonate were digested with trypsin (Promega, Madison, WI, USA) using a protein to enzyme ratio of 30:1 (w/w ...
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bioRxiv - Neuroscience 2024Quote: ... stable cell line expressing the human MC4-R and a split luciferase cAMP genetic sensor based on the circularized fusion of firefly luciferase and protein kinase A regulatory cAMP binding domain (clone 22F, Promega, Madison WI) was used to determine the α-MSH-elicited cAMP responses in live cells as previously described 32 ...
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bioRxiv - Cell Biology 2020Quote: ... One 10-cm plate of HEK-293T cells at 90% confluency was transfected with 6.5 μg of the pBMN-ARHGAP36 (isoform 2)-mCherry mutant library and 4 μg of pCL-ECO using the FuGene HD transfection reagent (Promega). The medium was replaced after 24 hours with DMEM containing 1.8 mM L-glutamine ...
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bioRxiv - Molecular Biology 2021Quote: ... were inserted using XhoI and NotI sites in 3’ UTR of Renilla gene in the psiCheck-2 dual luciferase reporter vector (Promega). The reporter in the amount of 25 ng and plasmids with different NORAD variants in the amount of 200 ng were introduced per 30,000 cells in 24-well plates ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were subjected to digestion of Lys-C (Wako Chemicals) (1:50) for 2 h at RT and then to trypsin (Promega) (1:50 ...