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Citations for Promega :
2201 - 2250 of 6667 citations for Osteopontin Human OPN ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Porcine trypsin (1:50, w/w) (Promega) was added ...
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bioRxiv - Developmental Biology 2023Quote: ... and 0.5 U μl-1 RNAsin (Promega)] ...
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bioRxiv - Neuroscience 2023Quote: ... and 1:1000 RNaisin Plus (Promega #N2615) before use] ...
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bioRxiv - Biochemistry 2024Quote: ... 1 μg of sequencing-grade trypsin (Promega) and 125 μL of digestion buffer (50 mM TEAB ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 U/μl RNAsin Plus (Promega) and flash frozen in LN2 (Hopes et al ...
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bioRxiv - Synthetic Biology 2024Quote: ... The BenchTop 1-kb DNA Ladder (Promega) was used to estimate the size of DNA.
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bioRxiv - Systems Biology 2024Quote: ... followed by 1:100 of trypsin (Promega) overnight at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg of sequencing grade trypsin (Promega), diluted in 50 mM ammonium bicarbonate ...
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bioRxiv - Cancer Biology 2021Quote: ... the samples were diluted to 1 M Urea with 10mM EPPS pH 8.2 and incubated overnight with 1:50 LysC (Wako Chemicals) and 1:100 Sequencing grade trypsin (Promega). Digests were acidified using TFA and tryptic peptides were purified by tC18 SepPak (50 mg ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was diluted either 1:5 or 1:10 for all qPCR experiments and GoTaq qPCR Master Mix (Promega) was used to amplify cDNA ...
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bioRxiv - Cell Biology 2022Quote: ... Beads were washed three times for 10 min with gentle rotation in high-salt buffer (50 mM Tris, pH 7.5, 300 mM KCl, 12 mM MgCl2, 1% Nonidet P-40, 1 mM DTT, 100U/mL Promega RNasin ...
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bioRxiv - Cell Biology 2022Quote: ... polysome were stabilised by washing cells in 20 ml ice-cold water containing 0.1 mg/ml CHX before being resuspended in 1 ml RiboTag Lysis Buffer (50 mM Tris, pH 7.5, 100 mM KCl, 12 mM MgCl2, 1% Nonidet P-40, 1 mM DTT, 100U/mL Promega RNasin ...
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bioRxiv - Biophysics 2020Quote: ... All samples were brought to room temperature and mixed with a 1:1 volume of nGlow solution (Promega, #N1110). Samples (90% of total volume ...
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bioRxiv - Cancer Biology 2021Quote: ... the samples were diluted to 1 M Urea with 10 mM EPPS pH 8.2 and incubated overnight with 1:50 LysC (Wako Chemicals) and 1:100 Sequencing grade trypsin (Promega). Digests were acidified using TFA and tryptic peptides were purified by Oasis Prime HLB columns (30 mg ...
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bioRxiv - Biophysics 2021Quote: ... All samples were brought to room temperature and mixed with a 1:1 volume of nGlow solution (Promega, #N1110). Samples (90% of total volume ...
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bioRxiv - Developmental Biology 2022Quote: HEK293T cells were simultaneously transfected with 1 μg pCS2Cachd1-eGFP or pCS2Cachd1_V1122D-eGFP and 1 μg KDEL-tRFP plasmid using Fugene 6 transfection reagent (Promega). eGFP/tRFP expression was confirmed 24 hours post transfection and the cells fixed at 42 hours post transfection and imaged on a spinning disk microscope (see below).
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bioRxiv - Molecular Biology 2019Quote: ... Cell pellets were lysed in 300µl of Lysis Buffer (50mM Tris-HCl pH 7.5, 150mM NaCl, 1% Triton® X-100, 0.1% deoxycholate, Protease Inhibitor and RNasin Plus [1:500, Promega]),diluted to a final concentration of ~1µg/µl with 100mM Tris-HCl pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cell media was then replaced with a 1:1 mixture of fresh media and Nano-Glo Luciferase reagent (Promega) and luminescence was measured using a SpectroMax M5e (Molecular Devices) ...
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bioRxiv - Immunology 2021Quote: ... The media from infected cells was then removed and 100 μl of 1:1 diluted PBS:Bio-Glo (Promega, G7940) luciferase substrate was added to each well ...
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bioRxiv - Cancer Biology 2020Quote: ... the samples were diluted to 1 M Urea with 10mM EPPS pH 8.2 and incubated overnight with 1:50 LysC (Wako Chemicals) and 1:100 Sequencing grade trypsin (Promega). Digests were acidified using TFA and tryptic peptideswere purified by tC18 SepPak (50 mg ...
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bioRxiv - Microbiology 2021Quote: ... the cells were lysed with a 1:1 ratio of virus to Bright-Glo luciferase assay media (Promega, #E2610) and the contents were analyzed on a luminometer (LUMISTAR Omega ...
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bioRxiv - Microbiology 2022Quote: ... cells were lysed by adding 100 µl of 1:1 (v:v) mixture of phenol red-free DMEM and Bright-Glo substrate (Promega) and luminescence detected using a Glomax Navigator (Promega) ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mM DTT) in presence of 1 mM Adenylyl-imidodiphosphate (AMP-PNP) and RNase inhibitor RNAsin (0.5 U, Promega) for 25 min at 4°C with head-over-end rotation ...
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bioRxiv - Immunology 2022Quote: ... caspase-1 activity was quantified in supernatants using a commercially available assay (Caspase-GLO 1 Assay; Promega, Madison, WI) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were incubated with the primary antibodies (1D4 1:1500, Anti-Calnexin Sigma c4731 1:600, Anti-LgBiT Promega N7100 1:100 ...
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Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... Orsay Virus RNA1 level was quantified by qPCR using 1 μl of 1/5 diluted cDNA (GoTaq Promega A6001) and run on QuantStudio 3 Real Time PCR system ...
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Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... Orsay virus RNA1 level was quantified by qPCR using 1 μl of 1/5 diluted cDNA (GoTaq Promega A6001) and run on QuantStudio 3 Real Time PCR system (RNA1 qPCR primers GW194 and GW195)23.
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bioRxiv - Molecular Biology 2023Quote: ... Two μL of reverse transcription buffer 5X were added and the samples were incubated at 20°C for 15 min before addition of 1.5 μL of dNTP mix (1 mM of dATP, dGTP, dCTP, dUTP) and 1 μL of AMV enzyme (PROMEGA). Reverse transcription was performed at 42°C for 45 min and synthesized cDNA was recovered by ethanol precipitation as described above ...
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bioRxiv - Immunology 2023Quote: ... Media were removed and 100 μl of CTG reagent (CellTiter-Glo®, Promega GmbH, Germany; 1:1 with PBS) per well was added ...
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bioRxiv - Microbiology 2023Quote: ... CM was then harvested by centrifugation and active caspase-1 measured with the Caspase-Glo 1 Inflammasome Assay (Promega) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The reaction was initiated by the addition of 1-250 nM acylated peptide (Vivitide) and a 1:10,000 dilution of LgBiT subunit of the NanoBiT luciferase (Promega) and read using an Envision plate reader (XCite 2105 ...
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bioRxiv - Plant Biology 2023Quote: ... Sample C was treated with 1% Triton X-100 (TX100) followed by treatment with 1μg mL-1 trypsin (Promega). For the detergent treatment ...
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bioRxiv - Genomics 2022Quote: ... Nuclei were resuspended in 200 μL resuspension buffer (1x PBS, 1% BSA and 0.2U μl/1 RNasin Plus RNase Inhibitor (Promega)) and transferred to a 1.5 mL Eppendorf tube tube ...
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bioRxiv - Neuroscience 2023Quote: ... and Dounce homogenized in 500 µL of ice-cold RNase-free buffer polysome buffer (50 mM Tris pH 7.5, 100 mM KCl, 12 mM MgCl2, 1% NP-40, 1 mM DTT, 200U/ml Promega RNasin ...
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bioRxiv - Developmental Biology 2023Quote: ... a minimum of 1 µg of RNA was combined with 1 µl of Oligo (dT)15 primer (Promega, C110B) and nuclease-free water to make up a total of 5 µl and incubated at 70°C and 4°C for 5 min each ...
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bioRxiv - Developmental Biology 2023Quote: ... A minimum of 1 µg of RNA was combined with 1 µl of Oligo (dT)15 primer (Promega, C110B) and nuclease-free water to make up a total of 5 µl and incubated at 70°C and 4°C for 5 min each ...
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bioRxiv - Molecular Biology 2023Quote: ... The secondary antibody was a horseradish peroxidase polyclonal goat anti-mouse (from 1:5,000 to 1:10,000, depending on the primary antibody; Promega, W4021). Proteins were detected with the ECL chemiluminescence reagent (GE Healthcare ...
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bioRxiv - Physiology 2024Quote: ... Protein digestion was performed with Lysyl Endopeptidase (enzyme:substrate 1:100, 125-05061, Wako Pure Chemical Industries) and trypsin (enzyme:substrate 1:50, Promega V5113) overnight at 37°C with agitation (1,000 rpm) ...
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bioRxiv - Cell Biology 2024Quote: ... 125 ng of which was the reporter plasmid mixture (1:1 ratio of 8xGli-nanoluc/pGl3b to pGL4.53[luc2/PGK] (Promega)) ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were then diluted to 1 M urea and subjected to digestion with trypsin (Promega, 1:50 w/w) overnight at 37⁰C ...
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bioRxiv - Biophysics 2024Quote: ... we stained the cells with 1 ml of a 1:2,000 TMR Halo-ligand solution (Promega, Cat. No.: G8251) to fluorescently label the Halo-tagged proteins ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 µL of HiBit buffer (LgBit 1:200, Promega, N112A; Furimazine 1:100 Promega, N113A in nuclease-free water) were added ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 1 nM of linear template DNA) and supplemented with 1 µL of BODIPY-Lys-tRNALys (FluoroTect GreenLys, Promega) to incorporate fluorescently labelled lysine residues in the synthesized proteins ...
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bioRxiv - Microbiology 2020Quote: ... Both NAD+ and NADH were quantified using NAD/NADH™-GLO kit (Promega) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... Genomic DNA was extracted from leaves using Wizard® Genomic Purification kit (Promega). Plant Tissue Protocol (Manufacturer protocol 3.E. ...
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bioRxiv - Cancer Biology 2021Quote: ... Glucose quantification was conducted using glucose bioluminescent assay kit (Glucose Glo Assay, Promega) as described per manufacturer instruction ...
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bioRxiv - Cancer Biology 2021Quote: ... and real-time PCR using GoTaq 2-Step RT-qPCR kit (Promega, A6110). All measurements were normalized against Actin as the internal control using the 2-ΔΔCt method (Figure 1—source data 3 and Figure 3—source data 2) ...
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bioRxiv - Cancer Biology 2021Quote: ... and DNaseI treated by Maxwell 16 LEV simplyRNA Cells Kit (Promega, Cat# AS1270) and Maxwell nucleic-acid extraction instrument (Promega) ...
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bioRxiv - Microbiology 2020Quote: ... Total GSH was measured from the supernatant using the GSH-Glo kit (Promega) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Relative NADPH levels were then determined using the NADPH-Glo assay kit (Promega) as per manufacturer’s instructions.