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Citations for Promega :
2151 - 2200 of 6143 citations for Cortisone Chemiluminescent ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Real time quantitative PCR was performed using GoTaq qPCR Master mix kit (Promega) in a CFX Connect Real-Time PCR thermocycler (BioRad) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Bacterial DNA was purified using the Maxwell 16-cell DNA purification kit (Promega) and sequenced by Illumina NovaSeq technology (paired-end) ...
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bioRxiv - Genomics 2024Quote: DNA was extracted from blood samples with Wizard Genomic DNA Purification kit (Promega) and quality was verified in 1% agarose gel electrophoresis ...
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bioRxiv - Genomics 2024Quote: ... The reactions were purified with the ProNex Size-Selective Purification System kit (Promega) and the concentration was rechecked by fluorometry in the Qubit 3.0 instrument (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... cell viability assays were performed using the Cell Titer Glo 3D kit (Promega) according to manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... Cultures showing mOrange expression were subsequently miniprepped using a Wizard miniprep kit (Promega). To remove the mOrange insert and generate and empty cloning vector ...
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bioRxiv - Genetics 2024Quote: ... cell viability was determined using CellTiter-Glo Luminescence Cell viability Assay kit (Promega) by measuring luminescence (ATP level ...
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bioRxiv - Biochemistry 2024Quote: RNA isolation was accomplished utilizing an RNA extraction kit (Promega, Madison, WI, USA). The production of cDNA was performed through reverse transcription utilising the cDNA Synthesis Kit (Yeasen ...
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bioRxiv - Systems Biology 2023Quote: ... LentivirusL titer was quantified before the screens using CellTiter-Glo kit (Promega G7570).
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bioRxiv - Zoology 2024Quote: For RNA extraction we used the ReliaPrep RNA Cell Miniprep System (Promega Kit). For the preparation of the Column Wash Solution ...
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bioRxiv - Microbiology 2023Quote: ... and firefly luciferase activity was determined using the Luciferase Assay Kit (Promega #E1501) according to the manufacturer’s instructions on an Orion microplate luminometer (Berthold).
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bioRxiv - Microbiology 2023Quote: ... RNA was extracted with the SV Total RNA isolation kit (Promega, Wisconsin, USA). Growth curves and RNA extraction were repeated three times.
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bioRxiv - Cancer Biology 2024Quote: ... Cytotoxicity was assessed CytoTox 96® Non-Radioactive Cytotoxicity Assay kit (Promega, G1780) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Infectivity was measured either by luciferase using the Luciferase Assay System Kit (Promega) or by flow cytometry ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA conversion was performed using the GoScript™ Reverse Transcriptase kit (#A5001, Promega). Quantitative real-time PCR was conducted on a Bio-rad CFX96 Real-time system ...
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bioRxiv - Cancer Biology 2024Quote: RNA was extracted from cells with the Maxwell RSC simplyRNA Cells Kit (Promega) on a Maxwell RSC instrument and mRNA reverse transcribed with the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... Total RNA was extracted using an SV Total RNA Isolation System Kit (Promega). The integrity of isolated RNA was evaluated on an Agilent 2100 Bioanalyzer ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was isolated with the Maxwell 48 RSC simply RNA Tissue Kit (Promega) on a Maxwell RSC Instrument (Promega) ...
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bioRxiv - Biophysics 2024Quote: CaMKIIα activity was assessed using the ADP-Glo Kinase Assay kit (#V9101, Promega) with CaMKIIα holoenzyme (#PR4586C ...
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bioRxiv - Cancer Biology 2023Quote: ... cell viability was measured by CellTiter-Blue® Cell Viability Assay kit (Promega). Endogenous UDP release was detected by UDP-GloTM Glycosyltransferase Assay kit (Promega).
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bioRxiv - Plant Biology 2023Quote: ... GTPase assays were performed using the GTPase-Glo Assay Kit (Promega, cat. V7681) basically following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and RNA was quantified by Quantifluor RNA kit (Promega, Charbonnières-les-bains, France). First ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were then digested with 1 μg LysC (Wako) overnight at room temperature and then with 1 μg sequencing grade trypsin (Promega) for 6 hours at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: The pGL-AP-1 plasmid containing 6 consensus AP-1 binding sites was co-transfected with the pRL-CMV plasmid (Promega) into cells using TransIT 2020 transfection reagent (Mirus Bio ...
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bioRxiv - Cell Biology 2019Quote: ... The reaction was diluted 1:4 with 50 mM ammonium bicarbonate and incubated with 1 µg of Trypsin Gold (Promega) at 37°C for more than 18 h before desalting ...
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bioRxiv - Cell Biology 2021Quote: ... Anti-mouse HRP conjugate (W4021, 1:10,000) and Anti-Rabbit HRP Conjugate (W4011, 1:10,000) were purchased from Promega (Madison, WI). Anti-COPI antibody was a gift from Charles Barlowe (Dartmouth Univ ...
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bioRxiv - Cell Biology 2020Quote: ... HRP Conjugate (W4011, 1:5000), and anti-Mouse IgG (H+L), HRP Conjugate (W4021, 1:5000) were purchased from Promega.
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bioRxiv - Cell Biology 2021Quote: ... Trypsin digestion was carried out overnight at 37°C with 1:50-1:100 enzyme-protein ratio of sequencing grade-modified trypsin (Promega) in 50 mM NH4HCO3 pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... The samples were diluted with 225 μL of 57.6 mM NH4HCO3 containing 1 mM TCEP and the mixtures treated with 1 μg of sequencing grade modified trypsin (Promega), incubated at 37 °C overnight and then acidified by addition of formic acid to 0.1 % v/v and stored at −20 °C ...
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bioRxiv - Cancer Biology 2019Quote: The activity of the inhibitors of hPD-1/hPD-L1 immune checkpoint was examined using the hPD-1/hPD-L1 Blockade Bioassay (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Cells for gene targeting were transfected with 500 ng of donor and gRNA plasmids in ratio 1:1 using ViaFect (Promega) transfection reagent according to the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2020Quote: ... the cells for the luciferase assay were exposed to 100 μL of a 1:1 dilution of DMEM and luciferase substrate (ONE-Glo Luciferase Assay System, Promega), and luminescence from each well measured in a GloMax-96 Microplate Luminometer (Promega) ...
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bioRxiv - Cell Biology 2020Quote: ... cells were seeded on 24 mm glass coverslips and transfected with 2 µg plasmid DNA (1:1.3:3 ratio LAMP1:ER:opto-kinesin) and Fugene6 transfection reagent (Promega 1:3) for 20-30 h prior to imaging ...
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bioRxiv - Cancer Biology 2019Quote: ... and Jurkat T cells overexpressing hPD-1 and carrying a luciferase reporter gene under the control of Nuclear Factor of Activated T-cells Response Element (NFAT-RE) (hPD-1 Effector Cells, hPD-1 ECs, Promega) were cultured in RPMI-1640 medium (Biowest ...
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bioRxiv - Immunology 2020Quote: ... pCAG-Flag or pCMV-HA-N expression vectors using standard molecular cloning methods as described in our previous publications.30–32 The IFN-β luciferase reporter plasmid pGL3-IFN-β-Luc vector was constructed in our previous study.33,34 The IFNλ1 luciferase reporter plasmid pGL3-IFNλ1-Luc was constructed by inserting the 1000-bp promoter region of human IFNλ1 (nucleotides −1000 to +1, with the translation start site set as 1) into pGL3-Basic (Promega, USA) according to methods outlined in previous studies.13,34 The ISG luciferase reporter plasmid pISRE-Luc vector was purchased from Clontech (USA) ...
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bioRxiv - Molecular Biology 2020Quote: ... and proteins were sequentially digested with LysC (1:50, w/w; FUJIFILM Wako) for 3 h at 37 °C and trypsin (50:1, w/w; Promega) overnight at 37 °C ...
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Intrarenal B cells integrate in situ innate and adaptive immunity in human renal allograft rejectionbioRxiv - Immunology 2020Quote: ... Trypsin digestion was carried out overnight at 37 °C with 1:50 - 1:100 enzyme-protein ratio of sequencing grade-modified trypsin (Promega) in 50 mM NH4HCO3 pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The sample was combined with 69 μL of ammonium bicarbonate along with 1 μL of 1% ProteaseMax surfactant (Promega V2072) for a total volume of 100 μL ...
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bioRxiv - Biophysics 2022Quote: ... Then the samples were diluted to 1 M urea with 50 mM (NH4)HCO3 (AB) and trypsinized (1:50 m/m, Promega) at 37 °C (overnight ...
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bioRxiv - Immunology 2022Quote: ... plates were equilibrated at room temperature for at least 30 minutes before 100 µL of 1:1 diluted PBS and CellTiter Glo reagent (Promega) was added to 100 µL of media ...
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bioRxiv - Molecular Biology 2023Quote: Cell pellets were lysed in cell lysis buffer (20 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% Triton-X with protease inhibitor cocktail (Promega)) and subjected to Western blotting ...
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bioRxiv - Cancer Biology 2022Quote: ... Urea was added to a final concentration of 1.6 M in 50 mM ammonium bicarbonate and 1 mM calcium chloride prior to digestion with 1 µg sequencing grade porcine trypsin (Promega) for 16 h at 37°C (74) ...
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bioRxiv - Immunology 2023Quote: ... samples were diluted 1:1 with water and digested for 1.5 hours at 37 °C with 1 µg of LysC and overnight at 37 °C with 1 µg trypsin (Promega). The peptide mixture was acidified with trifluoroacetic acid (Merck ...
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bioRxiv - Cancer Biology 2023Quote: ... 58 °C for 45 seconds and 72 °C for 1 minute (32 cycles) and 72 °C for 10 minutes (1 cycle) using GoTaq G2 DNA polymerase (Promega). Additionally ...
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bioRxiv - Biochemistry 2022Quote: ... Purified AaEnc and BSA were individually buffer exchanged into TBS pH 7.5 and mixed in a 40:1:1 molar ratio with commercially purchased trypsin (Promega, USA) and chymotrypsin (Promega ...
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bioRxiv - Neuroscience 2023Quote: ... urea concentration for each sample was diluted to <1 M using ammonium bicarbonate (ABC) and digested for an additional ∼16 hours using 1:50 (w/w) trypsin (Promega). Following digestion ...
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bioRxiv - Neuroscience 2023Quote: ... UDP (at concentrations ranging from 1 nM to 1 mM) was then applied with a final concentration of 5 μM furimazine (NanoLuc Luciferase Assay, Promega). Luminescence was subsequently measured using a Victor X5 multi-label plate reader (PerkinElmer).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the coding sequence of HIV-1 Nef (SF2 variant) was amplified by PCR and inserted into the plasmid pNLF-1-C (Promega) for expression of NanoLuc at the Nef C-terminus ...
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bioRxiv - Cell Biology 2023Quote: ... 100 mM KCl, 5 mM MgCl2, 2 mM DTT, 100 μg ml-1 cycloheximide, and 20 U ml-1 RNase inhibitor [Promega].) Gradients were centrifuged 36,000 rpm for 2.5 h in a SW41 Ti rotor (Beckman Coulter ...
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bioRxiv - Molecular Biology 2023Quote: ... cellular ATP concentrations were measured by adding 20 μL of 1x CellTiterGlo 2.0 (CTG) solution (1 part PBS, 1 part CellTiterGlo2.0 stock solution; Promega Cat# G9241) using a MultidropTM Combi liquid dispenser and measuring the luminescence on a SpectraMax iD3 (Molecular Devices).