Labshake search
Citations for Promega :
2051 - 2100 of 4570 citations for Human Immunodeficiency Virus Tat Protein HIV 1 Clade C TV019 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... IMR90 exosome proteins trapped in the S-Trap columns were digested with trypsin (Promega, Madison, WI, # V5111) in digestion buffer (50 mM TEAB ...
-
bioRxiv - Developmental Biology 2024Quote: Abd-B proteins for EMSA were produced using the TNT coupled in vitro transcription/translation system (Promega). Protein production was estimated by labeling the proteins with 35S-methionine and found to be produced at similar amounts ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: Comparison of VUF15485-induced β-arrestin2 recruitment to human and mouse ACKR3 was also measured by NanoLuc complementation assay (NanoBiT, Promega Corporation, Madison, WI, USA) in HEK293T cells (Dixon et al. ...
-
bioRxiv - Molecular Biology 2019Quote: ... tissue was immediately incubated in 1% PFA (with 1 µL mL−1 RNasin Plus (Promega, cat. #N2611)) for 5 minutes at 4°C ...
-
bioRxiv - Cell Biology 2021Quote: ... A polyclonal goat anti-mouse conjugated to horseradish peroxidase (1:5000, 1:10000 or 1:20000; Promega) was used as secondary antibody ...
-
bioRxiv - Cell Biology 2020Quote: ... 1 μg of pCS2-HA-NFATc3 was incubated for 2 h at 30°C in 50 μl of the TNT® SP6 coupled wheat germ extract system (Promega, #L5030), according to the instructions of the manufacturer ...
-
bioRxiv - Microbiology 2019Quote: ... cells were pelleted at 1000 rcf for 5 min at 4 °C and resuspended in luciferase reporter lysis buffer (Promega, Cat. #E4530) with 1% protease inhibitor cocktail (Millipore Sigma ...
-
bioRxiv - Molecular Biology 2021Quote: ... protein ratio of 1:50 w/w for 2 hours at 37°C and further digested with 30 µL 50 mM TEAB containing trypsin (Sequencing Grade Modified, Promega, Madison, WI) at a trypsin ...
-
bioRxiv - Genomics 2019Quote: ... cells were incubated at 37°C with MTS for 90 min following the instruction of CellTiter 96 AQueous One Solution Reagent (Promega, Madison, USA). The absorbance was measured at the wavelength of 490 nm by a Synergy H1 plate reader (BioTek ...
-
bioRxiv - Microbiology 2021Quote: ... Growth curves were obtained in 96 wells microplates at 37°C and automatic recording of OD600 every 30 minutes using a plate reader (Promega GloMax Explorer).
-
bioRxiv - Cancer Biology 2019Quote: Mitochondrial activity of C-33A cells was measured with the CellTiter 96 Aqueous One Solution Cell Proliferation Assay (MTS) (Promega, Madison, WI). Cells were seeded in 96-well plates (5000 cells/well ...
-
bioRxiv - Cell Biology 2021Quote: ... The resulting protein samples were diluted to a final concentration of 2M Urea using 20 mM HEPES (pH 8) and digested overnight at 37°C using sequencing grade modified Trypsin (Promega, Madison, WI) in a 1 ...
-
bioRxiv - Cell Biology 2021Quote: ... Primary antibodies were incubated in PBDS for 3 days at 4°C followed by 4 washes over the next 24 hours with 0.2% Tween-20 (Promega UK Ltd; H5151) in PBS ...
-
bioRxiv - Microbiology 2022Quote: ... The light production was then monitored at 20-minute intervals at 37 °C for 2 hours using the Glomax 20/20 Luminometer (Promega, Madison, WI). A final measurement was also taken after the samples had been allowed to incubate overnight at room temperature ...
-
bioRxiv - Microbiology 2020Quote: ... The cells were then incubated for 24 hours at 37°C in 5% CO2 and the luciferase activity was measured using the ONE-Glo™ luciferase assay (Promega, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... The cells were incubated at 37°C in 5% CO2 for 24 hours and luciferase activity was measured using a ONE-Glo™ luciferase assay (Promega, USA) according to the manufacturer’s instructions.
-
bioRxiv - Molecular Biology 2020Quote: ... for 30 min at 37°C with shaking at 500 rpm in 500 μl of PBS with RNasin® Ribonuclease Inhibitors (Promega, #N261B). Negative control samples comprised 5 μg of RNA combined with HisPur Cobalt Resin (see details below) ...
-
bioRxiv - Cell Biology 2020Quote: To compare Promega’s NanoBiT system β-arrestin2 was inserted at the C-terminal end of LgBiT using Promegas pBiT1.1-N vector (Promega Cat. #N2014) and the doubly palmitolyated fragment of GAP43 was inserted at the N-terminal of SmBiT using the pBiT2.1-C vector.
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were incubated with chemical treatments for 18 hours at 37°C prior to rinsing with PBS and lysing with 1x cell lysis buffer (Promega, Madison, WI). Luciferase activity was measured using a Berthold Centro XS3 LB 960 microplate luminometer with automatic injection of Luciferase Assay Reagent (Promega) ...
-
bioRxiv - Microbiology 2019Quote: ... we replaced the C-terminal V5 tag with either HaloTag or NanoLuc (NLuc) from the NanoBRET Nano-Glo Detection System (Promega, Madison, WI). As a negative control ...
-
bioRxiv - Microbiology 2020Quote: ... Proteins were digested O/N at 37°C with mass spectrometry grade lysyl endopeptidase LysC and sequencing grade modified trypsin (Promega LTD, UK).
-
bioRxiv - Pathology 2021Quote: ... 30 μg of protein was digested with Lys-C (FUJIFILM Wako Chemicals Europe GmbH, Germany) for 4 h and subsequently with modified porcine trypsin (Promega, WI, USA) for 16 h at 37 °C.
-
bioRxiv - Molecular Biology 2021Quote: ... cooled on ice to room temperature for 5 min and digested overnight at 37 °C with 0.5 μg of sequencing-grade trypsin (Promega Cat. No. V5111). Peptide mixtures were acidified to pH 3 with 1.5 µl 5% FA ...
-
bioRxiv - Microbiology 2021Quote: Protein samples were alkylated with 10 mM iodoacetamide in the dark at 37°C for 30m and subsequently digested with trypsin (Promega, 50 ng) overnight at 37°C ...
-
bioRxiv - Plant Biology 2022Quote: ... and alkylation treatment (100 mM iodoacetamide / 45 min incubation at room temperature) were followed by 16 hr incubation at 37°C with sequencing grade trypsin (Promega, Madison WI). Tryptic peptides were acidified with formic acid ...
-
bioRxiv - Molecular Biology 2022Quote: ... Pelleted cells were disrupted by glass beads agitation at 4°C in 1x Passive Lysis Buffer provided in the Dual-Luciferase® Reporter Assay System (Promega, #E1910). Extracts were clarified by centrifugation ...
-
bioRxiv - Zoology 2022Quote: ... The mixture was diluted with 150 μL of 50 mM Tris-HCl pH8.0 and digested by adding 500 ng Trypsin/Lys-C mix (Promega, Madison, WI, USA) overnight at 37 °C ...
-
bioRxiv - Microbiology 2022Quote: Genomic DNA was extracted at the NCPHL from subcultures inoculated with single bacterial colonies and grown in nutrient agar (Oxoid, USA) at 37°C overnight according to the manufacturer instructions (Wizard® Genomic DNA Purification kit, Promega, UK). Genomic DNA samples (derived from 10 environmental and 250 clinical samples ...
-
bioRxiv - Molecular Biology 2024Quote: ... The clear supernatant was incubated overnight at 4°C with 100 μl of prewashed Magne® HaloTag® Beads (Promega, WI, USA). Post-incubation ...
-
bioRxiv - Microbiology 2024Quote: ... culture supernatants were frozen at −s80°C and macrophages were processed according to the Promega E1500 Luciferase Assay System protocol (Promega, Madison, WI). Cells were lysed and incubated with D-Luciferin substrate for 5 min in white Lumitrac™ plates (Greiner Bio-one ...
-
bioRxiv - Molecular Biology 2022Quote: ... cDNA was synthesized with primers specific for each strand separately at 50 °C (Supplemental Table S1) using the ImProm-II™ Reverse Transcription System (Promega, A3800) and subjected to qPCR with primers specific for the viral N-protein (Supplemental Table S1).
-
bioRxiv - Biochemistry 2023Quote: One-hundred microliters of 50 mM Tris-HCl (pH 8.0) and 500 ng of trypsin/Lys-C mix (Promega, cat. no. V5072) were added to the washed HaloTag ligand plate following the first-generation assay and mixed gently at 37 °C overnight to digest the proteins ...
-
bioRxiv - Cancer Biology 2024Quote: ... tissues were incubated with primary antibodies diluted in 10% DS in PB1 for 3 days at 4°C followed by four washes over the following day with 0.2% Tween-20 (Promega UK Ltd, H5151) in PBS ...
-
bioRxiv - Biophysics 2019Quote: ... DBNL(1-256) or DBNL(1-374) using ViaFect (Promega) and assayed 24 h later ...
-
bioRxiv - Immunology 2020Quote: ... and mixed 1:1 v:v with BacTiter-Glo Reagent (Promega). The reaction was incubated for 5 min at room temperature on an orbital shaker ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1 mM dNTP) and 1 μl of AMV RT (Promega) was added and the sample was incubated at 42 °C for 1 hour ...
-
bioRxiv - Molecular Biology 2022Quote: ... cells were lysed in 1:1 PBS:CellTiter-Glo 2.0 (Promega) reagent ...
-
bioRxiv - Cell Biology 2024Quote: ... cDNA was diluted 1:1 with nuclease-free water (Promega). Quantitative real-time PCR reactions were carried out using the SYBR Green PCR master mix and ROX Passive Reference Dye (Bio-Rad ...
-
bioRxiv - Microbiology 2023Quote: ... and a 1:1 mixture of CellTiter-Glo reagent (Promega) and PBS was added to each well ...
-
bioRxiv - Bioengineering 2022Quote: ... 3 mM CaCl2 at pH 6.4) 0.2 µg/μL protein was mixed with proteases trypsin (Promega sequencing grade), chymotrypsin (BD) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Reduced and alkylated gel pieces were processed for in-gel protein digestion by trypsin (Promega, Madison WI, USA) overnight at 37 °C adding ammonium bicarbonate buffer to cover gel matrix ...
-
bioRxiv - Microbiology 2020Quote: ... dengue protein NS2B/3/4A constructs were expressed in rabbit reticulocyte lysate (TNT coupled T7, Promega Bio Systems) programmed with 1 µg DNA/50 µL and labeled with 20 µCi of L-[35S]-methionine (EasyTag ...
-
bioRxiv - Neuroscience 2019Quote: ... The level of BDNF protein from tissue extracts was determined with the BDNF Emax® ImmunoAssay System (Promega) using the manusfacturer’s instruction ...
-
bioRxiv - Genomics 2019Quote: ... 35S labeled proteins were synthesized in vitro using the TNT T7 Quick for PCR DNA kit (Promega, L5540). Labeled proteins were incubated with MBP-tagged proteins in TNEN50 (50mM Tris pH7.5 ...
-
Multimodal tubulin binding by the yeast kinesin-8, Kip3, underlies its motility and depolymerizationbioRxiv - Biophysics 2021Quote: ... The protein was fluorescently labeled with 10uM TMR (dimer constructs) or 30uM TMR (monomer constructs, HALO-TMR; Promega) on beads for 1h at 4°C ...
-
bioRxiv - Neuroscience 2019Quote: ... Samples were further diluted to a final urea concentration of 2 M and proteins digested with trypsin (Promega) (1/100 ...
-
bioRxiv - Cell Biology 2021Quote: ... the bead-bound protein was incubated with either 6.7 μM HaloTag-TMR or HaloTag-PEG-biotin ligand (Promega) for 15 minutes at room temperature ...
-
bioRxiv - Cell Biology 2021Quote: ... and the amount of the HiBiT protein was measured using the Nano Glo HiBiT Lytic Detection System (Promega). As a quantification standard for HiBiT proteins ...
-
bioRxiv - Microbiology 2021Quote: ATPase activity of the purified proteins was determined using two kits – Enliten ATPase assay (FF2000, Promega, Moscow, Russia) and malachite green phosphate assay (MAK307 ...
-
bioRxiv - Microbiology 2022Quote: ... and SV40 late poly(A) signal was amplified from pNL1.1.CMV[Nluc/CMV] (19.1 kDa NanoLuc protein, Promega). The PCR product was blunt end ligated to pAD1 digested with EcoRV ...